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The map label for this gene is miaA

Identifier: 182680630

GI number: 182680630

Start: 88318

End: 89271

Strand: Reverse

Name: miaA

Synonym: XfasM23_0059

Alternate gene names: 182680630

Gene position: 89271-88318 (Counterclockwise)

Preceding gene: 182680631

Following gene: 182680629

Centisome position: 3.52

GC content: 53.25

Gene sequence:

>954_bases
ATGCCGGCCGACACGAGACCAGCCGCAATTGTACTCATGGGGCCAACCGCAAGCGGCAAAAGCCAACTGGCCATTGATAT
TGCCAAGCGTTGGGGAGGGGAGGTCATTAGTGTTGACTCGGTTCTGGTCTATCGTGGCTTAGACATCGGTACTGCAAAGC
CGAACGCGGCAATGCGTGCATCAGTACCGCATCATTTGATCGACATTTGTGAACCTTGGGAAACCTATTCTGCTGCCGAT
TTCGCGCATGATGCACGCGCCGCAATCGACATGATCGTTAGACGTGGCGCATTACCAATCCTCACTGGCGGTACTGGACT
GTACTTCCGTGCGTTACTGGCAGGTCTTTCGGATATGCCTCCAGCTCATCCTGAAATACGCGCGATGATCGCGGCTGAAG
CCAAGAGAGACAGTTGGGCAGCACTACACACACGTTTGGCCGAAGTCGATGCAATCACGGCTGCACGTATCCACGCGACC
GACCCACAGCGAATTCAGCGTGCATTGGAAGTCTATCTAGTGAGCGGACAATCAATGAGTGATTGGCAAAATCAGCCACC
CAAACAACGATTACCTTTGCGGGTTCTTAAGCTGGTGCTCGCACCGACCCATCGCAAAGTACTGCACTTCAGGATCGCGC
AGCGCTTCAAGGCAATGTTGGATAACGGCCTACTTGCCGAGGTGAATGCGTTACGTACACACCCATCCATCCACGCAATG
GCTCGACCACTGGATCTTCCAGCAATGCGTGCGGTTGGCTACCGCCAATGTTGGGAACACTTGGACGGCATGTACACTGC
CGAAATGCTGTATCAGCGCAGCGTTGCCGCCACACGTCAACTGGCCAAACGTCAGTTAACGTGGCTACGTGGCGAGCTGG
ATGCCCTTTGGTTCGATCCAGAACATGATCAATCCAGAATAGAGAAAGTCATGGAAGCATTTTTAAACAGATAA

Upstream 100 bases:

>100_bases
CTATGGTCGATGCCCTCAAAGTTTGGCAGGCAGTTTCAGCAGCGGCTGCACCTGCAACACATACCAAAACTACAGTGGCT
CGTTCGCCCCATGACGACTG

Downstream 100 bases:

>100_bases
AAACATGTCGACTGCGTCTCAGCGGTGTAACATCATGGCTCCGGAGCCGATTAGGAAAACAAACCGGCTGACCGGAGTAA
TAATAAAAATAAAGGAGTTT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MPADTRPAAIVLMGPTASGKSQLAIDIAKRWGGEVISVDSVLVYRGLDIGTAKPNAAMRASVPHHLIDICEPWETYSAAD
FAHDARAAIDMIVRRGALPILTGGTGLYFRALLAGLSDMPPAHPEIRAMIAAEAKRDSWAALHTRLAEVDAITAARIHAT
DPQRIQRALEVYLVSGQSMSDWQNQPPKQRLPLRVLKLVLAPTHRKVLHFRIAQRFKAMLDNGLLAEVNALRTHPSIHAM
ARPLDLPAMRAVGYRQCWEHLDGMYTAEMLYQRSVAATRQLAKRQLTWLRGELDALWFDPEHDQSRIEKVMEAFLNR

Sequences:

>Translated_317_residues
MPADTRPAAIVLMGPTASGKSQLAIDIAKRWGGEVISVDSVLVYRGLDIGTAKPNAAMRASVPHHLIDICEPWETYSAAD
FAHDARAAIDMIVRRGALPILTGGTGLYFRALLAGLSDMPPAHPEIRAMIAAEAKRDSWAALHTRLAEVDAITAARIHAT
DPQRIQRALEVYLVSGQSMSDWQNQPPKQRLPLRVLKLVLAPTHRKVLHFRIAQRFKAMLDNGLLAEVNALRTHPSIHAM
ARPLDLPAMRAVGYRQCWEHLDGMYTAEMLYQRSVAATRQLAKRQLTWLRGELDALWFDPEHDQSRIEKVMEAFLNR
>Mature_316_residues
PADTRPAAIVLMGPTASGKSQLAIDIAKRWGGEVISVDSVLVYRGLDIGTAKPNAAMRASVPHHLIDICEPWETYSAADF
AHDARAAIDMIVRRGALPILTGGTGLYFRALLAGLSDMPPAHPEIRAMIAAEAKRDSWAALHTRLAEVDAITAARIHATD
PQRIQRALEVYLVSGQSMSDWQNQPPKQRLPLRVLKLVLAPTHRKVLHFRIAQRFKAMLDNGLLAEVNALRTHPSIHAMA
RPLDLPAMRAVGYRQCWEHLDGMYTAEMLYQRSVAATRQLAKRQLTWLRGELDALWFDPEHDQSRIEKVMEAFLNR

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=308, Percent_Identity=29.2207792207792, Blast_Score=125, Evalue=4e-29,
Organism=Escherichia coli, GI1790613, Length=306, Percent_Identity=49.3464052287582, Blast_Score=288, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI25144712, Length=237, Percent_Identity=28.6919831223629, Blast_Score=88, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6324848, Length=293, Percent_Identity=29.6928327645051, Blast_Score=125, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24649830, Length=261, Percent_Identity=34.0996168582375, Blast_Score=135, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_XYLF2 (B2I692)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001828790.1
- ProteinModelPortal:   B2I692
- SMR:   B2I692
- GeneID:   6202337
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0059
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 35362; Mature: 35231

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPADTRPAAIVLMGPTASGKSQLAIDIAKRWGGEVISVDSVLVYRGLDIGTAKPNAAMRA
CCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCEEEECCEEEEECCCCCCCCCCCCCCC
SVPHHLIDICEPWETYSAADFAHDARAAIDMIVRRGALPILTGGTGLYFRALLAGLSDMP
CCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCC
PAHPEIRAMIAAEAKRDSWAALHTRLAEVDAITAARIHATDPQRIQRALEVYLVSGQSMS
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHCCCCCHH
DWQNQPPKQRLPLRVLKLVLAPTHRKVLHFRIAQRFKAMLDNGLLAEVNALRTHPSIHAM
HHHCCCCHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHH
ARPLDLPAMRAVGYRQCWEHLDGMYTAEMLYQRSVAATRQLAKRQLTWLRGELDALWFDP
HCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
EHDQSRIEKVMEAFLNR
CCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
PADTRPAAIVLMGPTASGKSQLAIDIAKRWGGEVISVDSVLVYRGLDIGTAKPNAAMRA
CCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCEEEECCEEEEECCCCCCCCCCCCCCC
SVPHHLIDICEPWETYSAADFAHDARAAIDMIVRRGALPILTGGTGLYFRALLAGLSDMP
CCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCC
PAHPEIRAMIAAEAKRDSWAALHTRLAEVDAITAARIHATDPQRIQRALEVYLVSGQSMS
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHCCCCCHH
DWQNQPPKQRLPLRVLKLVLAPTHRKVLHFRIAQRFKAMLDNGLLAEVNALRTHPSIHAM
HHHCCCCHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHH
ARPLDLPAMRAVGYRQCWEHLDGMYTAEMLYQRSVAATRQLAKRQLTWLRGELDALWFDP
HCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
EHDQSRIEKVMEAFLNR
CCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA