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The map label for this gene is hmp [H]

Identifier: 182680608

GI number: 182680608

Start: 49987

End: 51180

Strand: Direct

Name: hmp [H]

Synonym: XfasM23_0036

Alternate gene names: 182680608

Gene position: 49987-51180 (Clockwise)

Preceding gene: 182680606

Following gene: 182680609

Centisome position: 1.97

GC content: 47.4

Gene sequence:

>1194_bases
ATGTCTGCATCATTTTCGCCACATACAATTACACTTATAAAATCTACAGTGCCATTATTGGCCGAACATGGCACAACTAT
CATCGAAGCCATGTACCATCGATTGTTCGAAGATCCGCAGATTGAGGCGCTGTTCAATCAGGCAAACCAGAAGAACGGCA
CTCAAATCCATGCACTTGCTGGAGCGATACTGGCGTACGCTCGCAATATTGATAACCCGGGTGTGCTTGCATCAGCGATT
GAAAGAATTTCACAAAAGCATGTTGGTTATGCAATCCATCCAGAACATTATCCTCATGTCGCAACCGCTTTACTGGGTGC
GATTAAGCAAGTCTTGGGTGACGTTGCCACATCAGAAGTGTTGGAAGCCTGGGGAGAAGCGTATTGGTTTATTGCAAATC
TTTTAAAAGATCGGGAAGCAGTGATTCGTGAGGGGATCATGACCAAGAACGGTGGGTGGATCCACTGGCGGCGTTTTGTC
ATTTCCAAGCGTATTCCTGAAAGTGAAACGATTACTTCGTTTATGCTGCATCCTGAGGATGGTGGGCCGGTTGTCCCCCA
TCAGGCCGGACAATATCTTACGTTTCGTTTCGATGCAGCCGGCATGCCGGGTATGAAACGCAATTACTCTATTTCCTGTG
GTCCTAATAGCGACCATTATCGGATTACCGTTAAACGTGAACACGGCACAGGGGCATCGGCTTTTCTACACGATCAAGCG
AAAGTTGGAACGATTATCGAATGTACGCCGCCTGTGGGTGATTTTTTCCTTCCCAGTGTGATTGAAAGGCCAATCGTTCT
TTTATCTGGTGGTGTCGGATTAACACCAATGGTGAGCATGATGGAGCAGATCGCTGAAGCGTATCCTGATGCGCAGGTAT
GGTATGTCCATGGTACTCAGAATCGAGAAACACATGCGATGGATGCTCATATTCGTGCTTTAGTGAGTCGACACAAGCAT
ATGAAAGCCACGACGTTCTATACACAACGTAGTGAAGCCGATGATGCGGAAGCCGGGTTTATCACCATTGATTGGTTGCG
AGCCAATACACCGTTTCAAAAGGCCGATTTTTACCTTTGTGGCCCGCGTCCCTTCTTGCGCACTTTTGTTCGTGATCTGA
TCGGTGCTGGTGTGCCAGCAGCACAGGTTCATTATGAATTTTTCGGTCCGATGGATGAAGAAATGGCCGCATAA

Upstream 100 bases:

>100_bases
TGATGGGGTGTGTATAAAAATAATTTAAATGAATTTTGAATGAGGGGCGATGTATCATCCGTTCGCGGCATTCAAGAACT
CATGTCGAAGGAATTAATAG

Downstream 100 bases:

>100_bases
TGGTGTGATCCTACTAGATAAATTCAAGGCATTGCATTGTTTCATATCGTCTATTATTTCTATTAATAAATGCGCGGAAA
AAATTCCATTGTTGTCGTGG

Product: nitric oxide dioxygenase

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MSASFSPHTITLIKSTVPLLAEHGTTIIEAMYHRLFEDPQIEALFNQANQKNGTQIHALAGAILAYARNIDNPGVLASAI
ERISQKHVGYAIHPEHYPHVATALLGAIKQVLGDVATSEVLEAWGEAYWFIANLLKDREAVIREGIMTKNGGWIHWRRFV
ISKRIPESETITSFMLHPEDGGPVVPHQAGQYLTFRFDAAGMPGMKRNYSISCGPNSDHYRITVKREHGTGASAFLHDQA
KVGTIIECTPPVGDFFLPSVIERPIVLLSGGVGLTPMVSMMEQIAEAYPDAQVWYVHGTQNRETHAMDAHIRALVSRHKH
MKATTFYTQRSEADDAEAGFITIDWLRANTPFQKADFYLCGPRPFLRTFVRDLIGAGVPAAQVHYEFFGPMDEEMAA

Sequences:

>Translated_397_residues
MSASFSPHTITLIKSTVPLLAEHGTTIIEAMYHRLFEDPQIEALFNQANQKNGTQIHALAGAILAYARNIDNPGVLASAI
ERISQKHVGYAIHPEHYPHVATALLGAIKQVLGDVATSEVLEAWGEAYWFIANLLKDREAVIREGIMTKNGGWIHWRRFV
ISKRIPESETITSFMLHPEDGGPVVPHQAGQYLTFRFDAAGMPGMKRNYSISCGPNSDHYRITVKREHGTGASAFLHDQA
KVGTIIECTPPVGDFFLPSVIERPIVLLSGGVGLTPMVSMMEQIAEAYPDAQVWYVHGTQNRETHAMDAHIRALVSRHKH
MKATTFYTQRSEADDAEAGFITIDWLRANTPFQKADFYLCGPRPFLRTFVRDLIGAGVPAAQVHYEFFGPMDEEMAA
>Mature_396_residues
SASFSPHTITLIKSTVPLLAEHGTTIIEAMYHRLFEDPQIEALFNQANQKNGTQIHALAGAILAYARNIDNPGVLASAIE
RISQKHVGYAIHPEHYPHVATALLGAIKQVLGDVATSEVLEAWGEAYWFIANLLKDREAVIREGIMTKNGGWIHWRRFVI
SKRIPESETITSFMLHPEDGGPVVPHQAGQYLTFRFDAAGMPGMKRNYSISCGPNSDHYRITVKREHGTGASAFLHDQAK
VGTIIECTPPVGDFFLPSVIERPIVLLSGGVGLTPMVSMMEQIAEAYPDAQVWYVHGTQNRETHAMDAHIRALVSRHKHM
KATTFYTQRSEADDAEAGFITIDWLRANTPFQKADFYLCGPRPFLRTFVRDLIGAGVPAAQVHYEFFGPMDEEMAA

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=390, Percent_Identity=39.4871794871795, Blast_Score=278, Evalue=4e-76,
Organism=Saccharomyces cerevisiae, GI6321673, Length=406, Percent_Identity=32.7586206896552, Blast_Score=193, Evalue=4e-50,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.17 [H]

Molecular weight: Translated: 44090; Mature: 43959

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS01033 GLOBIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASFSPHTITLIKSTVPLLAEHGTTIIEAMYHRLFEDPQIEALFNQANQKNGTQIHALA
CCCCCCCCEEEEEHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHH
GAILAYARNIDNPGVLASAIERISQKHVGYAIHPEHYPHVATALLGAIKQVLGDVATSEV
HHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
LEAWGEAYWFIANLLKDREAVIREGIMTKNGGWIHWRRFVISKRIPESETITSFMLHPED
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHHEEEEEECCC
GGPVVPHQAGQYLTFRFDAAGMPGMKRNYSISCGPNSDHYRITVKREHGTGASAFLHDQA
CCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCCEEEEEEEECCCCCCHHEEECCC
KVGTIIECTPPVGDFFLPSVIERPIVLLSGGVGLTPMVSMMEQIAEAYPDAQVWYVHGTQ
CCCEEEEECCCCCCHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEECCC
NRETHAMDAHIRALVSRHKHMKATTFYTQRSEADDAEAGFITIDWLRANTPFQKADFYLC
CCCHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCCCCCEEEEEEEECCCCCCCCCEEEE
GPRPFLRTFVRDLIGAGVPAAQVHYEFFGPMDEEMAA
CCCHHHHHHHHHHHHCCCCHHHEEHHHCCCCHHHHCC
>Mature Secondary Structure 
SASFSPHTITLIKSTVPLLAEHGTTIIEAMYHRLFEDPQIEALFNQANQKNGTQIHALA
CCCCCCCEEEEEHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHH
GAILAYARNIDNPGVLASAIERISQKHVGYAIHPEHYPHVATALLGAIKQVLGDVATSEV
HHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
LEAWGEAYWFIANLLKDREAVIREGIMTKNGGWIHWRRFVISKRIPESETITSFMLHPED
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHHEEEEEECCC
GGPVVPHQAGQYLTFRFDAAGMPGMKRNYSISCGPNSDHYRITVKREHGTGASAFLHDQA
CCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCCEEEEEEEECCCCCCHHEEECCC
KVGTIIECTPPVGDFFLPSVIERPIVLLSGGVGLTPMVSMMEQIAEAYPDAQVWYVHGTQ
CCCEEEEECCCCCCHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEECCC
NRETHAMDAHIRALVSRHKHMKATTFYTQRSEADDAEAGFITIDWLRANTPFQKADFYLC
CCCHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCCCCCEEEEEEEECCCCCCCCCEEEE
GPRPFLRTFVRDLIGAGVPAAQVHYEFFGPMDEEMAA
CCCHHHHHHHHHHHHCCCCHHHEEHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]