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The map label for this gene is truA

Identifier: 182677494

GI number: 182677494

Start: 555948

End: 556730

Strand: Direct

Name: truA

Synonym: Bind_0498

Alternate gene names: 182677494

Gene position: 555948-556730 (Clockwise)

Preceding gene: 182677493

Following gene: 182677499

Centisome position: 13.33

GC content: 62.2

Gene sequence:

>783_bases
ATGCCCCGCTATAAATTAACTGTCGAATATGACGGCGCGTCCTTTGTGGGTTGGCAGAGGCAGAAGAATGGGCTCTCGGT
TCAGGAAGTGATCGAGACGGCTCTCTTGGCCATCAATGGCGCGCCGGTTGGCATTCGCGGAGCGGGCCGCACGGATGCCG
GCGTTCATGCGAGTGCTCAAGTGGCCCATGCCGATCTTGCGCGGGAATGGCGTCCCGATGTCCTGCGCGATGCCTTGAAC
GCGCATATGCGTCCGGCTTTGGTCGCTGTCGTGGCGGCGGAACCTGTCCCGGAAACGTTCGACGCGCGCTTTTCAGCCAT
CCGCCGGCATTATCTCTACCGTATCATCAATCGCCGTGCGCCTCTGACCTTTGAGGCGGGGCGCGCCTGGCTGGTTAAAC
GCCGGCTCGATGCGCGAGCCATGCATGAAGCGGCGCAAGTCCTGACCGGACGCCATGATTTTTCGACCTTTCGGGCGGCG
GAATGCCAGGCGGCGAGCCCAATCCGCACGCTGGAGCGTTTGGACGTGCAAGCCGTGGGCGATCTCATCGAAATCCGGGC
GAGCGCGCGATCCTTCCTGCATCATCAGGTGCGCTCGATGGTGGGGTCGCTCGAAATGGTCGGTTCCGGCAAATGGAGCG
CAGGGGATCTGCGGGCGGCTCTGGAGGCCTGTGATCGGCGCCGTTGTGGCATGGTCGCTCCGGCCGCCGGCCTTTATCTG
ACCGGGGTCGATTATCCTGAAGAAGGAGAAGAAAAAGGGGCTCAGCCCTTTTTCGGCGAATAG

Upstream 100 bases:

>100_bases
CTCGATATTCAACGGTCCGGCAAAGCACCCATGAGCGCCGAGGCCTTTTTGCGCGGCACGAAACTGCCTGCCAGCACCCG
ATTGCCGCTGCCCGATCTCC

Downstream 100 bases:

>100_bases
CCGATCTGCAGGGATTCCTGCGCGTTCTGAGTCAGGACATTCTGGATCTTGCCGGAGAGCGTTGCGAGAATCCAGGGCAA
TTGCACTTCGAGCCGAATGA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MPRYKLTVEYDGASFVGWQRQKNGLSVQEVIETALLAINGAPVGIRGAGRTDAGVHASAQVAHADLAREWRPDVLRDALN
AHMRPALVAVVAAEPVPETFDARFSAIRRHYLYRIINRRAPLTFEAGRAWLVKRRLDARAMHEAAQVLTGRHDFSTFRAA
ECQAASPIRTLERLDVQAVGDLIEIRASARSFLHHQVRSMVGSLEMVGSGKWSAGDLRAALEACDRRRCGMVAPAAGLYL
TGVDYPEEGEEKGAQPFFGE

Sequences:

>Translated_260_residues
MPRYKLTVEYDGASFVGWQRQKNGLSVQEVIETALLAINGAPVGIRGAGRTDAGVHASAQVAHADLAREWRPDVLRDALN
AHMRPALVAVVAAEPVPETFDARFSAIRRHYLYRIINRRAPLTFEAGRAWLVKRRLDARAMHEAAQVLTGRHDFSTFRAA
ECQAASPIRTLERLDVQAVGDLIEIRASARSFLHHQVRSMVGSLEMVGSGKWSAGDLRAALEACDRRRCGMVAPAAGLYL
TGVDYPEEGEEKGAQPFFGE
>Mature_259_residues
PRYKLTVEYDGASFVGWQRQKNGLSVQEVIETALLAINGAPVGIRGAGRTDAGVHASAQVAHADLAREWRPDVLRDALNA
HMRPALVAVVAAEPVPETFDARFSAIRRHYLYRIINRRAPLTFEAGRAWLVKRRLDARAMHEAAQVLTGRHDFSTFRAAE
CQAASPIRTLERLDVQAVGDLIEIRASARSFLHHQVRSMVGSLEMVGSGKWSAGDLRAALEACDRRRCGMVAPAAGLYLT
GVDYPEEGEEKGAQPFFGE

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=276, Percent_Identity=36.5942028985507, Blast_Score=127, Evalue=7e-30,
Organism=Escherichia coli, GI1788657, Length=249, Percent_Identity=40.5622489959839, Blast_Score=169, Evalue=1e-43,
Organism=Drosophila melanogaster, GI116007752, Length=307, Percent_Identity=29.9674267100977, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_BEII9 (B2IEW0)

Other databases:

- EMBL:   CP001016
- RefSeq:   YP_001831640.1
- GeneID:   6198728
- GenomeReviews:   CP001016_GR
- KEGG:   bid:Bind_0498
- HOGENOM:   HBG621020
- OMA:   AIKRHYL
- ProtClustDB:   PRK00021
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 28586; Mature: 28455

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRYKLTVEYDGASFVGWQRQKNGLSVQEVIETALLAINGAPVGIRGAGRTDAGVHASAQ
CCCEEEEEEECCCCEECCHHCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCHHH
VAHADLAREWRPDVLRDALNAHMRPALVAVVAAEPVPETFDARFSAIRRHYLYRIINRRA
HHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCC
PLTFEAGRAWLVKRRLDARAMHEAAQVLTGRHDFSTFRAAECQAASPIRTLERLDVQAVG
CCEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHH
DLIEIRASARSFLHHQVRSMVGSLEMVGSGKWSAGDLRAALEACDRRRCGMVAPAAGLYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEE
TGVDYPEEGEEKGAQPFFGE
ECCCCCCCCHHCCCCCCCCC
>Mature Secondary Structure 
PRYKLTVEYDGASFVGWQRQKNGLSVQEVIETALLAINGAPVGIRGAGRTDAGVHASAQ
CCEEEEEEECCCCEECCHHCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCHHH
VAHADLAREWRPDVLRDALNAHMRPALVAVVAAEPVPETFDARFSAIRRHYLYRIINRRA
HHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCC
PLTFEAGRAWLVKRRLDARAMHEAAQVLTGRHDFSTFRAAECQAASPIRTLERLDVQAVG
CCEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHH
DLIEIRASARSFLHHQVRSMVGSLEMVGSGKWSAGDLRAALEACDRRRCGMVAPAAGLYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEE
TGVDYPEEGEEKGAQPFFGE
ECCCCCCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA