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The map label for this gene is nifH [H]

Identifier: 182677469

GI number: 182677469

Start: 533912

End: 534784

Strand: Direct

Name: nifH [H]

Synonym: Bind_0473

Alternate gene names: 182677469

Gene position: 533912-534784 (Clockwise)

Preceding gene: 182677463

Following gene: 182677470

Centisome position: 12.8

GC content: 59.79

Gene sequence:

>873_bases
ATGCGCCAGATTGCGTTCTACGGTAAGGGTGGTATCGGCAAGTCCACCACCTCGCAAAACACCCTCGCCTGCCTCGCCCA
GATGGGCCACAAGATCCTGATCGTCGGCTGCGACCCCAAGGCTGACTCGACCCGCCTGATCCTTCACTCGAAGGCCCAAG
ACACCATCCTCAGCCTCGCCGCTGCCGCTGGTTCCGTCGAAGACCTCGAAATCGAAGACGTCATGAAGGTCGGCTACCTC
GACATCAAGTGCGTTGAGTCCGGTGGTCCGGAGCCGGGCGTCGGCTGCGCCGGTCGTGGCGTTATCACCTCGATCAACTT
CCTCGAGGAAAACGGCGCTTACGAAGACGTTGACTACGTTTCCTACGACGTGCTCGGCGACGTGGTCTGCGGTGGCTTCG
CCATGCCGATCCGTGAGAACAAGGCTCAGGAAATCTACATCGTCATGTCTGGCGAGATGATGGCCATGTATGCGGCCAAC
AACATCTCCAAGGGCATTCTGAAGTATGCCAACTCGGGCGGCGTGCGTCTCGGCGGTCTCGTCTGCAACGAGCGTCAGAC
CGACAAGGAACTCGAGCTGGCTGAAGAGCTTGCCAAGCGCCTCGGCACCAAGCTGATCCACTTCGTTCCCCGCGACAACA
TCGTGCAGCATGCCGAACTCCGTCGTATGACGGTTGTCGAATATGCTCCCGATTCCAAGCAGGCTGACGAGTATCGTACG
CTCGCCAACAAGGTCCATGCGAACAAGGGCAACGGCATCATCCCGACCCCGATCACCATGGACGAGCTCGAGGACATCCT
CATGGCTCACGGCATCATGAAGCAGGTTGACGAGTCGCAGGTCGGCCTCACCGCCGCTCAGCTCTCGGCCTAA

Upstream 100 bases:

>100_bases
CCGCCATGGCGTAGCGATGCCTGATGCAGGAACTGTGGCAGGAATCATGCGTCTCGGGTCCACGGTTAAATAGCAAAACG
GCACTAAGGAGATCTTTTAA

Downstream 100 bases:

>100_bases
TGCCCTTAAGCCGGCCCCTCTCAGGAGGGGCCGGTCTTTACTGCAAGACGAATACTTCAGGAGATTGGCATGAGCCTATC
GGCACCCGAAACAATCGAAG

Product: nitrogenase reductase

Products: NA

Alternate protein names: Nitrogenase Fe protein 1; Nitrogenase component II; Nitrogenase reductase [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MRQIAFYGKGGIGKSTTSQNTLACLAQMGHKILIVGCDPKADSTRLILHSKAQDTILSLAAAAGSVEDLEIEDVMKVGYL
DIKCVESGGPEPGVGCAGRGVITSINFLEENGAYEDVDYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMAMYAAN
NISKGILKYANSGGVRLGGLVCNERQTDKELELAEELAKRLGTKLIHFVPRDNIVQHAELRRMTVVEYAPDSKQADEYRT
LANKVHANKGNGIIPTPITMDELEDILMAHGIMKQVDESQVGLTAAQLSA

Sequences:

>Translated_290_residues
MRQIAFYGKGGIGKSTTSQNTLACLAQMGHKILIVGCDPKADSTRLILHSKAQDTILSLAAAAGSVEDLEIEDVMKVGYL
DIKCVESGGPEPGVGCAGRGVITSINFLEENGAYEDVDYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMAMYAAN
NISKGILKYANSGGVRLGGLVCNERQTDKELELAEELAKRLGTKLIHFVPRDNIVQHAELRRMTVVEYAPDSKQADEYRT
LANKVHANKGNGIIPTPITMDELEDILMAHGIMKQVDESQVGLTAAQLSA
>Mature_290_residues
MRQIAFYGKGGIGKSTTSQNTLACLAQMGHKILIVGCDPKADSTRLILHSKAQDTILSLAAAAGSVEDLEIEDVMKVGYL
DIKCVESGGPEPGVGCAGRGVITSINFLEENGAYEDVDYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMAMYAAN
NISKGILKYANSGGVRLGGLVCNERQTDKELELAEELAKRLGTKLIHFVPRDNIVQHAELRRMTVVEYAPDSKQADEYRT
LANKVHANKGNGIIPTPITMDELEDILMAHGIMKQVDESQVGLTAAQLSA

Specific function: The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components:the iron protein and the molybdenum-iron protein [H]

COG id: COG1348

COG function: function code P; Nitrogenase subunit NifH (ATPase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NifH/BchL/ChlL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000392
- InterPro:   IPR005977 [H]

Pfam domain/function: PF00142 Fer4_NifH [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 31269; Mature: 31269

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00746 NIFH_FRXC_1 ; PS00692 NIFH_FRXC_2 ; PS51026 NIFH_FRXC_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQIAFYGKGGIGKSTTSQNTLACLAQMGHKILIVGCDPKADSTRLILHSKAQDTILSLA
CCEEEEECCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEEEECCHHHHHHHHH
AAAGSVEDLEIEDVMKVGYLDIKCVESGGPEPGVGCAGRGVITSINFLEENGAYEDVDYV
HHCCCCCCCCHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCEEEEEEHHCCCCCCCCHHH
SYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMAMYAANNISKGILKYANSGGVRLGGL
HHHHHHHHHHCCEECCCCCCCCCEEEEEECCCEEEEEECCCHHHHHHHHCCCCCEEEEEE
VCNERQTDKELELAEELAKRLGTKLIHFVPRDNIVQHAELRRMTVVEYAPDSKQADEYRT
EECCCCCCHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHEEEEEECCCCCCHHHHHH
LANKVHANKGNGIIPTPITMDELEDILMAHGIMKQVDESQVGLTAAQLSA
HHHHHCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCHHHHCEEHHHHCC
>Mature Secondary Structure
MRQIAFYGKGGIGKSTTSQNTLACLAQMGHKILIVGCDPKADSTRLILHSKAQDTILSLA
CCEEEEECCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEEEECCHHHHHHHHH
AAAGSVEDLEIEDVMKVGYLDIKCVESGGPEPGVGCAGRGVITSINFLEENGAYEDVDYV
HHCCCCCCCCHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCEEEEEEHHCCCCCCCCHHH
SYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMAMYAANNISKGILKYANSGGVRLGGL
HHHHHHHHHHCCEECCCCCCCCCEEEEEECCCEEEEEECCCHHHHHHHHCCCCCEEEEEE
VCNERQTDKELELAEELAKRLGTKLIHFVPRDNIVQHAELRRMTVVEYAPDSKQADEYRT
EECCCCCCHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHEEEEEECCCCCCHHHHHH
LANKVHANKGNGIIPTPITMDELEDILMAHGIMKQVDESQVGLTAAQLSA
HHHHHCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCHHHHCEEHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA