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The map label for this gene is nifB [H]

Identifier: 182677392

GI number: 182677392

Start: 456878

End: 458461

Strand: Direct

Name: nifB [H]

Synonym: Bind_0396

Alternate gene names: 182677392

Gene position: 456878-458461 (Clockwise)

Preceding gene: 182677391

Following gene: 182677393

Centisome position: 10.96

GC content: 59.47

Gene sequence:

>1584_bases
ATGGATTTGACAGAACGCGAAGTCGCGGAAGCGGCGATCAAGGCGGGCGCGAACGCCGACGAAATCATGAAGAAAATGGC
CGAGCACAAGGGCTGCGAGACCGACAGCGACGGCGGCAAGAAGGCCAGCTGCGGTACGGCTGCTGGTGAGAACGATCTTC
CGCCGGAAATCTGGGAAAAGGTCAAGAACCATCCCTGCTACAGCGAAGAAGCGCATCATCACTATGCTCGTATGCACGTT
GCCGTGGCTCCGGCCTGCAACATTCAGTGCAACTATTGCAACCGCAAATATGATTGCGCCAACGAGTCCCGTCCGGGCGT
CGTCTCCGAGCGTCTGACGCCGGAACAAGCCGCTAAGAAGGTTCTCGCGGTTGCTTCCACCATCCCGCAAATGACCGTTC
TCGGCATTGCCGGCCCCGGCGACCCACTCGCCAATCCGGAAAAGACCTTCAAGACCTTCGACTTGATCGCGAAGACCGCT
CCGGACATCAAGCTCTGCCTGTCCACCAACGGCCTGACGCTGCCCGACCATGTCGACACGATCGCCCGTTACAATGTCGA
TCACGTCACGATCACCGTGAACATGGTTGATGCGGAAGTCGGCGCCAAGATCTATCCCTGGATCTTCTACAAGCATAAAC
GCTACACCGGCAAGGAGGCCGCCCAGATCCTGACCGACCGTCAGATGCAGGGCCTTGAAATGCTGACCGAGCGCGGCATT
CTCTCCAAGATCAACTCGGTGATGATCCCCGGCATCAACGACGAGCATCTGAAGGAAGTCAACAAGGCTGTCAAAGGCCG
TGGCGCCTTCCTGCACAACATCATGCCGCTGATCTCCGCCGCCGAGCACGGCACGGTCTTCGGTCTGACCGGCCAGCGCG
GTCCGACGGCGCAAGAACTGAAGCGCCTGCAGGACAGCTGCGAAGGTGAGATGAACATGATGCGTCACTGCCGTCAGTGC
CGCGCGGATGCCGTCGGCCTCCTCGGTGAGGACCGCAGCGCCGAGTTCACCACCGAAAAGATCATGGAGATGGACGTCAA
CTACGATCTCGCGACCCGTCAGGCCTATCAAGCCCGCGTCGAAGAAGAGCGTAATGCCGTTGCCGGCGCCAAGGCTGCCG
AACTCCAGATCCTGGCCGATGTCGAAAGCGACATCAAGATCCTCGTCGCGGTTGCCACCAAGGGCAGCGGCCGCATCAAC
GAGCATTTCGGTCATGCGTCTGAATTCCAGATCTATGAGCTGAGCACAGGCAAGGGTGCCCACTTCATCGGTCACCGCCG
CGTCGACCTGTATTGCCAGGGCGGCTACGGCGAGGAAGACGCTCTCGAGACCGTCATCCGCGCCATCAAGGACTGCCATG
CGGTGTTCGTCGCCAAGATCGGCGGCTGCCCGAAGAAGGACCTTCTCGCGGCCGGTATCGAAGCGATCGACGAATTCGCT
CATGAGTTCATCGAGAAGTCCGCGATCACCTACTTCAAGCGTTATCTCGAAGGCGTGAAGGATGGTTCGATCACTCACGT
TGAAAAGGGTGATGCCGAAATCCGTCCCGGCGAAGTCGCGGTGGACGTGGTCGCTGCTGAATAA

Upstream 100 bases:

>100_bases
TTCGTTGAGCGGGATCGTAGACGAGCTTATCGGCGGCGGGGGGAGAGTGCGGTGCGCGCCCCTTTTAACCAATAGGTTCC
AGCCAGAAGCGAGGATCAAC

Downstream 100 bases:

>100_bases
TCCCTTAAGCGATCTCTATCGGGGCGCTTCGGCGCCCCGATATACGGGTTACCCGCACCTTTTCTCCTGATCCTTTTCAA
GGAGCGATTCAATGTCATTG

Product: nitrogenase cofactor biosynthesis protein NifB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MDLTEREVAEAAIKAGANADEIMKKMAEHKGCETDSDGGKKASCGTAAGENDLPPEIWEKVKNHPCYSEEAHHHYARMHV
AVAPACNIQCNYCNRKYDCANESRPGVVSERLTPEQAAKKVLAVASTIPQMTVLGIAGPGDPLANPEKTFKTFDLIAKTA
PDIKLCLSTNGLTLPDHVDTIARYNVDHVTITVNMVDAEVGAKIYPWIFYKHKRYTGKEAAQILTDRQMQGLEMLTERGI
LSKINSVMIPGINDEHLKEVNKAVKGRGAFLHNIMPLISAAEHGTVFGLTGQRGPTAQELKRLQDSCEGEMNMMRHCRQC
RADAVGLLGEDRSAEFTTEKIMEMDVNYDLATRQAYQARVEEERNAVAGAKAAELQILADVESDIKILVAVATKGSGRIN
EHFGHASEFQIYELSTGKGAHFIGHRRVDLYCQGGYGEEDALETVIRAIKDCHAVFVAKIGGCPKKDLLAAGIEAIDEFA
HEFIEKSAITYFKRYLEGVKDGSITHVEKGDAEIRPGEVAVDVVAAE

Sequences:

>Translated_527_residues
MDLTEREVAEAAIKAGANADEIMKKMAEHKGCETDSDGGKKASCGTAAGENDLPPEIWEKVKNHPCYSEEAHHHYARMHV
AVAPACNIQCNYCNRKYDCANESRPGVVSERLTPEQAAKKVLAVASTIPQMTVLGIAGPGDPLANPEKTFKTFDLIAKTA
PDIKLCLSTNGLTLPDHVDTIARYNVDHVTITVNMVDAEVGAKIYPWIFYKHKRYTGKEAAQILTDRQMQGLEMLTERGI
LSKINSVMIPGINDEHLKEVNKAVKGRGAFLHNIMPLISAAEHGTVFGLTGQRGPTAQELKRLQDSCEGEMNMMRHCRQC
RADAVGLLGEDRSAEFTTEKIMEMDVNYDLATRQAYQARVEEERNAVAGAKAAELQILADVESDIKILVAVATKGSGRIN
EHFGHASEFQIYELSTGKGAHFIGHRRVDLYCQGGYGEEDALETVIRAIKDCHAVFVAKIGGCPKKDLLAAGIEAIDEFA
HEFIEKSAITYFKRYLEGVKDGSITHVEKGDAEIRPGEVAVDVVAAE
>Mature_527_residues
MDLTEREVAEAAIKAGANADEIMKKMAEHKGCETDSDGGKKASCGTAAGENDLPPEIWEKVKNHPCYSEEAHHHYARMHV
AVAPACNIQCNYCNRKYDCANESRPGVVSERLTPEQAAKKVLAVASTIPQMTVLGIAGPGDPLANPEKTFKTFDLIAKTA
PDIKLCLSTNGLTLPDHVDTIARYNVDHVTITVNMVDAEVGAKIYPWIFYKHKRYTGKEAAQILTDRQMQGLEMLTERGI
LSKINSVMIPGINDEHLKEVNKAVKGRGAFLHNIMPLISAAEHGTVFGLTGQRGPTAQELKRLQDSCEGEMNMMRHCRQC
RADAVGLLGEDRSAEFTTEKIMEMDVNYDLATRQAYQARVEEERNAVAGAKAAELQILADVESDIKILVAVATKGSGRIN
EHFGHASEFQIYELSTGKGAHFIGHRRVDLYCQGGYGEEDALETVIRAIKDCHAVFVAKIGGCPKKDLLAAGIEAIDEFA
HEFIEKSAITYFKRYLEGVKDGSITHVEKGDAEIRPGEVAVDVVAAE

Specific function: Probably involved in the synthesis of the Fe-Mo cofactor [H]

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moaA/nifB/pqqE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003731
- InterPro:   IPR000385
- InterPro:   IPR005980
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02579 Nitro_FeMo-Co; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 57707; Mature: 57707

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLTEREVAEAAIKAGANADEIMKKMAEHKGCETDSDGGKKASCGTAAGENDLPPEIWEK
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
VKNHPCYSEEAHHHYARMHVAVAPACNIQCNYCNRKYDCANESRPGVVSERLTPEQAAKK
HHCCCCCCCHHHHHHHHEEEEECCCCCEEECCCCCCCCCCCCCCCCCHHHCCCHHHHHHH
VLAVASTIPQMTVLGIAGPGDPLANPEKTFKTFDLIAKTAPDIKLCLSTNGLTLPDHVDT
HHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHH
IARYNVDHVTITVNMVDAEVGAKIYPWIFYKHKRYTGKEAAQILTDRQMQGLEMLTERGI
HHHCCCCEEEEEEEEEEHHHCCEEECEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCHH
LSKINSVMIPGINDEHLKEVNKAVKGRGAFLHNIMPLISAAEHGTVFGLTGQRGPTAQEL
HHHHCCEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHH
KRLQDSCEGEMNMMRHCRQCRADAVGLLGEDRSAEFTTEKIMEMDVNYDLATRQAYQARV
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHH
EEERNAVAGAKAAELQILADVESDIKILVAVATKGSGRINEHFGHASEFQIYELSTGKGA
HHHHHHHCCCCHHHEEEEECCCCCEEEEEEEECCCCCCCHHHCCCCCCEEEEEECCCCCC
HFIGHRRVDLYCQGGYGEEDALETVIRAIKDCHAVFVAKIGGCPKKDLLAAGIEAIDEFA
EEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
HEFIEKSAITYFKRYLEGVKDGSITHVEKGDAEIRPGEVAVDVVAAE
HHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEEEEECC
>Mature Secondary Structure
MDLTEREVAEAAIKAGANADEIMKKMAEHKGCETDSDGGKKASCGTAAGENDLPPEIWEK
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
VKNHPCYSEEAHHHYARMHVAVAPACNIQCNYCNRKYDCANESRPGVVSERLTPEQAAKK
HHCCCCCCCHHHHHHHHEEEEECCCCCEEECCCCCCCCCCCCCCCCCHHHCCCHHHHHHH
VLAVASTIPQMTVLGIAGPGDPLANPEKTFKTFDLIAKTAPDIKLCLSTNGLTLPDHVDT
HHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHH
IARYNVDHVTITVNMVDAEVGAKIYPWIFYKHKRYTGKEAAQILTDRQMQGLEMLTERGI
HHHCCCCEEEEEEEEEEHHHCCEEECEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCHH
LSKINSVMIPGINDEHLKEVNKAVKGRGAFLHNIMPLISAAEHGTVFGLTGQRGPTAQEL
HHHHCCEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHH
KRLQDSCEGEMNMMRHCRQCRADAVGLLGEDRSAEFTTEKIMEMDVNYDLATRQAYQARV
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHH
EEERNAVAGAKAAELQILADVESDIKILVAVATKGSGRINEHFGHASEFQIYELSTGKGA
HHHHHHHCCCCHHHEEEEECCCCCEEEEEEEECCCCCCCHHHCCCCCCEEEEEECCCCCC
HFIGHRRVDLYCQGGYGEEDALETVIRAIKDCHAVFVAKIGGCPKKDLLAAGIEAIDEFA
EEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
HEFIEKSAITYFKRYLEGVKDGSITHVEKGDAEIRPGEVAVDVVAAE
HHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1840608 [H]