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The map label for this gene is nifA [H]

Identifier: 182677389

GI number: 182677389

Start: 453040

End: 454923

Strand: Direct

Name: nifA [H]

Synonym: Bind_0393

Alternate gene names: 182677389

Gene position: 453040-454923 (Clockwise)

Preceding gene: 182677388

Following gene: 182677390

Centisome position: 10.86

GC content: 59.18

Gene sequence:

>1884_bases
ATGAACGATCAGGATCATGCGGAAGCCGCCAAGACAATGCCCTCCCACCGCATTCAACTGAGTGAGATCGCCCTTATAGG
CGTCTATGAAATCTCTAAAATTCTGACTGCGCCGACCCGTCTTGAGACGACGCTCGCCAATGTCTTGAACCTGCTTTCGT
CCTTCCTGCAGATGCGGCATGGCACGATCGTGTTGCTAGCCGATGACGGCGCGCCGGAAGTGGCGGTCGGAGCCGGCTGG
ACGGAAGAGGCCATGCCGGCGCCACAACGCTATCCGGAAAGGGCGATCGGCCAGATCGTGGCGACGGCCGTGCCGCTTGT
CGTGCATAATGTCGCCGATCATGAACTCTTCGACGCGAATGATGTCGCGGCGCTCGCCTCAGGGGGCGCGAAGGTCTCCT
TCATGGGTGTGCCGATCCGGGCCGGCGATCGCGTTGTCGGCACGCTCACAGTCGATCGCATCTGGGATGGCGAAAGCGTT
TTCCGTTTCGATTCGGACGTGCGTTTCCTCGTGATGATCGCCAATCTGATCGGTCAAACGGTGAAATTGCATCGTGTCGT
CGCGCGTGACCGCGATCGTTTGATCGAGGAAAGCCATCGTCTGCAAAAGGAAATCACCAAATTGCAGCCGATGCCGGTCA
GCAAGGCGCGTGCTTCTGGTATCATTGGTGAAAGTCCGGCGATCCGTGCCGTTCTCGACAAGATTTCCATTGTCGCGCGT
TCCAATGCGACCATGCTGCTGCGCGGTGAATCAGGCACAGGCAAGGAACTCTTCGCGCGTGCCTTGCATGAAATGTCACC
GCGCGCCTCCCATGCCTTCGTCAAGGTCAATTGCGCGGCTCTTGCCGAGAGCGTTCTTGAATCGGAATTATTCGGTCATG
AGAAAGGCGCGTTTACTGGCGCCGTGGCGACCCGCAAGGGCCGTTTTGAACTGGCCGATGGTGGCACGTTATTTCTGGAT
GAAATTGGCGAAATTTCGCTCTCTTTCCAGGCGAAGCTTTTGCGTGTGCTGCAAGAGGGTGAATTCGAGCGGGTCGGCGG
CACCAAGACTCTCAAGGCCGATGTGCGTTTGATCACGGCCACCAACAAAAACCTTGAGGAAGCCGTGCGCAACGGCGAAT
TCCGCGCCGATCTTTATTATCGTATCAGCGTCGTGCCGGTGATATTGCCGCCCCTGCGGGAGCGCAGTAGCGATATTCCC
TTGCTCGCCGCGCGTTTCCTCGAACAATTCAACGAGGTCAACGGACGCGATCTGGTTTTCAGCAAACAGGCGATGGAAGT
GCTGAAATCCTGCTATTTTCCGGGCAATGTCCGAGAGCTCGAAAATTGCGTGCAACGGACCGCGACTTTCGCTGTGGGCG
AGTCCATCGTGGCAACCGATTTCGCCTGCGGCCAGGATCAATGCCTTTCAGCGACACTCTGGAAGGGCAAGACCTCGGCC
GAGGCCTGGCCGAGCAAAGCGATCGGTGGTCTTGGACCTTTCGGCTTGCCGGGCGGCCTGACGGAGCCTCCCCATCAGTC
GCATCCGCATCATTTGGAGGCGCAGCATTTGGCGCATCCGCAGGCCTATTCGGCGCCGCCACTCGCGCCGCCCCCCGTCG
CCGTGCCGCATATGCCGGCTTCCCCCGTGCCCGCCTCTTCTCATCCGCATGTCCCGCTTCCTCCGGTCACGCCGAAACCG
GCTCCGAATGTCCAGGATGTCTGGGATGAAGGTGGAGTCCTGGTGGATGTTGGTGGTGGCGGCGATACAGAGCGCGCGCG
GCTCCTGGAAGCGATGGAAAAAGCCGGTTGGGTTCAGGCCAAAGCCGCTCGCATCATGGGATTGACGCCGCGCCAGATCG
GCTATGCCCTGCGTAAGCACGGGATCGAGATCAAAAAATTCTAG

Upstream 100 bases:

>100_bases
GGTGAGCTCTTTAATCCAGTTTCAGCATAGCATCGGGAATGCGCCGAGACGATGCATCAAGACGATGCCATGCAGCCTTT
GAGGCTTTGGAGACCGCTCG

Downstream 100 bases:

>100_bases
TATCCACCTTCATTGAAGCGCGACTTGTCGCAGTTCAATGAGGATAAGTGGATAAGTTTAAAAAGCGACATATCGAGAAT
CTGTGATTCTCGATACTAGC

Product: transcriptional regulator NifA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 627; Mature: 627

Protein sequence:

>627_residues
MNDQDHAEAAKTMPSHRIQLSEIALIGVYEISKILTAPTRLETTLANVLNLLSSFLQMRHGTIVLLADDGAPEVAVGAGW
TEEAMPAPQRYPERAIGQIVATAVPLVVHNVADHELFDANDVAALASGGAKVSFMGVPIRAGDRVVGTLTVDRIWDGESV
FRFDSDVRFLVMIANLIGQTVKLHRVVARDRDRLIEESHRLQKEITKLQPMPVSKARASGIIGESPAIRAVLDKISIVAR
SNATMLLRGESGTGKELFARALHEMSPRASHAFVKVNCAALAESVLESELFGHEKGAFTGAVATRKGRFELADGGTLFLD
EIGEISLSFQAKLLRVLQEGEFERVGGTKTLKADVRLITATNKNLEEAVRNGEFRADLYYRISVVPVILPPLRERSSDIP
LLAARFLEQFNEVNGRDLVFSKQAMEVLKSCYFPGNVRELENCVQRTATFAVGESIVATDFACGQDQCLSATLWKGKTSA
EAWPSKAIGGLGPFGLPGGLTEPPHQSHPHHLEAQHLAHPQAYSAPPLAPPPVAVPHMPASPVPASSHPHVPLPPVTPKP
APNVQDVWDEGGVLVDVGGGGDTERARLLEAMEKAGWVQAKAARIMGLTPRQIGYALRKHGIEIKKF

Sequences:

>Translated_627_residues
MNDQDHAEAAKTMPSHRIQLSEIALIGVYEISKILTAPTRLETTLANVLNLLSSFLQMRHGTIVLLADDGAPEVAVGAGW
TEEAMPAPQRYPERAIGQIVATAVPLVVHNVADHELFDANDVAALASGGAKVSFMGVPIRAGDRVVGTLTVDRIWDGESV
FRFDSDVRFLVMIANLIGQTVKLHRVVARDRDRLIEESHRLQKEITKLQPMPVSKARASGIIGESPAIRAVLDKISIVAR
SNATMLLRGESGTGKELFARALHEMSPRASHAFVKVNCAALAESVLESELFGHEKGAFTGAVATRKGRFELADGGTLFLD
EIGEISLSFQAKLLRVLQEGEFERVGGTKTLKADVRLITATNKNLEEAVRNGEFRADLYYRISVVPVILPPLRERSSDIP
LLAARFLEQFNEVNGRDLVFSKQAMEVLKSCYFPGNVRELENCVQRTATFAVGESIVATDFACGQDQCLSATLWKGKTSA
EAWPSKAIGGLGPFGLPGGLTEPPHQSHPHHLEAQHLAHPQAYSAPPLAPPPVAVPHMPASPVPASSHPHVPLPPVTPKP
APNVQDVWDEGGVLVDVGGGGDTERARLLEAMEKAGWVQAKAARIMGLTPRQIGYALRKHGIEIKKF
>Mature_627_residues
MNDQDHAEAAKTMPSHRIQLSEIALIGVYEISKILTAPTRLETTLANVLNLLSSFLQMRHGTIVLLADDGAPEVAVGAGW
TEEAMPAPQRYPERAIGQIVATAVPLVVHNVADHELFDANDVAALASGGAKVSFMGVPIRAGDRVVGTLTVDRIWDGESV
FRFDSDVRFLVMIANLIGQTVKLHRVVARDRDRLIEESHRLQKEITKLQPMPVSKARASGIIGESPAIRAVLDKISIVAR
SNATMLLRGESGTGKELFARALHEMSPRASHAFVKVNCAALAESVLESELFGHEKGAFTGAVATRKGRFELADGGTLFLD
EIGEISLSFQAKLLRVLQEGEFERVGGTKTLKADVRLITATNKNLEEAVRNGEFRADLYYRISVVPVILPPLRERSSDIP
LLAARFLEQFNEVNGRDLVFSKQAMEVLKSCYFPGNVRELENCVQRTATFAVGESIVATDFACGQDQCLSATLWKGKTSA
EAWPSKAIGGLGPFGLPGGLTEPPHQSHPHHLEAQHLAHPQAYSAPPLAPPPVAVPHMPASPVPASSHPHVPLPPVTPKP
APNVQDVWDEGGVLVDVGGGGDTERARLLEAMEKAGWVQAKAARIMGLTPRQIGYALRKHGIEIKKF

Specific function: Required for activation of most nif operons, which are directly involved in nitrogen fixation [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=365, Percent_Identity=40.8219178082192, Blast_Score=270, Evalue=2e-73,
Organism=Escherichia coli, GI1788550, Length=262, Percent_Identity=51.1450381679389, Blast_Score=266, Evalue=4e-72,
Organism=Escherichia coli, GI1789087, Length=321, Percent_Identity=44.2367601246106, Blast_Score=261, Evalue=7e-71,
Organism=Escherichia coli, GI87082152, Length=416, Percent_Identity=41.5865384615385, Blast_Score=261, Evalue=7e-71,
Organism=Escherichia coli, GI1790437, Length=246, Percent_Identity=54.0650406504065, Blast_Score=260, Evalue=2e-70,
Organism=Escherichia coli, GI1790299, Length=248, Percent_Identity=50.4032258064516, Blast_Score=242, Evalue=5e-65,
Organism=Escherichia coli, GI1788905, Length=255, Percent_Identity=46.2745098039216, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI1786524, Length=244, Percent_Identity=48.7704918032787, Blast_Score=208, Evalue=9e-55,
Organism=Escherichia coli, GI87081872, Length=231, Percent_Identity=48.9177489177489, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI1789233, Length=229, Percent_Identity=45.4148471615721, Blast_Score=199, Evalue=6e-52,
Organism=Escherichia coli, GI1787583, Length=232, Percent_Identity=43.9655172413793, Blast_Score=183, Evalue=3e-47,
Organism=Escherichia coli, GI1789828, Length=249, Percent_Identity=38.5542168674699, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI87081858, Length=238, Percent_Identity=31.5126050420168, Blast_Score=123, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR010113
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 67838; Mature: 67838

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDQDHAEAAKTMPSHRIQLSEIALIGVYEISKILTAPTRLETTLANVLNLLSSFLQMRH
CCCHHHHHHHHCCCCCCEEEHHEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
GTIVLLADDGAPEVAVGAGWTEEAMPAPQRYPERAIGQIVATAVPLVVHNVADHELFDAN
CEEEEEECCCCCCEEECCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
DVAALASGGAKVSFMGVPIRAGDRVVGTLTVDRIWDGESVFRFDSDVRFLVMIANLIGQT
CHHHHHCCCCEEEEEEEEECCCCEEEEEEEEEEEECCCCEEEECCCCHHHHHHHHHHHHH
VKLHRVVARDRDRLIEESHRLQKEITKLQPMPVSKARASGIIGESPAIRAVLDKISIVAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHEEE
SNATMLLRGESGTGKELFARALHEMSPRASHAFVKVNCAALAESVLESELFGHEKGAFTG
CCCEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCEEE
AVATRKGRFELADGGTLFLDEIGEISLSFQAKLLRVLQEGEFERVGGTKTLKADVRLITA
HEECCCCCEEECCCCEEEEECCCCEEHHHHHHHHHHHHCCCCHHCCCCEEEEECEEEEEE
TNKNLEEAVRNGEFRADLYYRISVVPVILPPLRERSSDIPLLAARFLEQFNEVNGRDLVF
CCCCHHHHHHCCCEEEEEEEEEEEEEEECCCHHCCCCCCHHHHHHHHHHHHCCCCCEEEE
SKQAMEVLKSCYFPGNVRELENCVQRTATFAVGESIVATDFACGQDQCLSATLWKGKTSA
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHCCHHHCCCCCCC
EAWPSKAIGGLGPFGLPGGLTEPPHQSHPHHLEAQHLAHPQAYSAPPLAPPPVAVPHMPA
CCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCC
SPVPASSHPHVPLPPVTPKPAPNVQDVWDEGGVLVDVGGGGDTERARLLEAMEKAGWVQA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCHHH
KAARIMGLTPRQIGYALRKHGIEIKKF
HHHHHHCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNDQDHAEAAKTMPSHRIQLSEIALIGVYEISKILTAPTRLETTLANVLNLLSSFLQMRH
CCCHHHHHHHHCCCCCCEEEHHEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
GTIVLLADDGAPEVAVGAGWTEEAMPAPQRYPERAIGQIVATAVPLVVHNVADHELFDAN
CEEEEEECCCCCCEEECCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
DVAALASGGAKVSFMGVPIRAGDRVVGTLTVDRIWDGESVFRFDSDVRFLVMIANLIGQT
CHHHHHCCCCEEEEEEEEECCCCEEEEEEEEEEEECCCCEEEECCCCHHHHHHHHHHHHH
VKLHRVVARDRDRLIEESHRLQKEITKLQPMPVSKARASGIIGESPAIRAVLDKISIVAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHEEE
SNATMLLRGESGTGKELFARALHEMSPRASHAFVKVNCAALAESVLESELFGHEKGAFTG
CCCEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCEEE
AVATRKGRFELADGGTLFLDEIGEISLSFQAKLLRVLQEGEFERVGGTKTLKADVRLITA
HEECCCCCEEECCCCEEEEECCCCEEHHHHHHHHHHHHCCCCHHCCCCEEEEECEEEEEE
TNKNLEEAVRNGEFRADLYYRISVVPVILPPLRERSSDIPLLAARFLEQFNEVNGRDLVF
CCCCHHHHHHCCCEEEEEEEEEEEEEEECCCHHCCCCCCHHHHHHHHHHHHCCCCCEEEE
SKQAMEVLKSCYFPGNVRELENCVQRTATFAVGESIVATDFACGQDQCLSATLWKGKTSA
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHCCHHHCCCCCCC
EAWPSKAIGGLGPFGLPGGLTEPPHQSHPHHLEAQHLAHPQAYSAPPLAPPPVAVPHMPA
CCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCC
SPVPASSHPHVPLPPVTPKPAPNVQDVWDEGGVLVDVGGGGDTERARLLEAMEKAGWVQA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCHHH
KAARIMGLTPRQIGYALRKHGIEIKKF
HHHHHHCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3313281; 12597275; 3357773; 2792368 [H]