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The map label for this gene is bioH [C]

Identifier: 182677307

GI number: 182677307

Start: 354810

End: 355784

Strand: Direct

Name: bioH [C]

Synonym: Bind_0310

Alternate gene names: 182677307

Gene position: 354810-355784 (Clockwise)

Preceding gene: 182677305

Following gene: 182677309

Centisome position: 8.51

GC content: 58.05

Gene sequence:

>975_bases
ATGAGAACTATGAAAGCCGAACGCCGAGCCTTCCTAATCGGGACCTCAGGGATCATCTTTGGACCGGCGCTGGCGATGAT
GTGCGCACCGGGTGCGATGGCTCAGTCGATGGCCTTGAGGAAAGCGCCTGACGCAACACTTCCTAAAGGCGGAACGAGCC
AATTCGTGCAGATCAACGGCATCAGGTTGCACTATGTCAGCACGGGATCGGGTCCTGCGGTGATCTTGCTCCATGGGTGG
CCGCAAACATGGTTCGCTTGGCGAGCGACGATGGAACGGCTCTCCTCACATTTCACAGTTATTGCGCCTGATCTCAGAGG
CGTCGGGCTATCTGAACGGACGCCGACGGGCTACGATAAACGGACTATCGCAAGCGATATCGCCGCTCTCATTGGTCATG
CTGCGGGCGGCCGGGCTCATGTCGTGGCACACGATATGGGCGGCAAGGTAGCATATATGCTCGCCAATCTTCATCCCGAA
AGCGTCGAGAAGCTCGTTCTGGTCGATTGCCTCATTCCAGGAACAGAAAACATGGACGCCCTACGCGGAGGAGCGTGGCA
TTATGGGTTTCACATGGCTCCAGAGATTCCCGAGATGCTGACCAAGGGAAGGGAGCGCGACTACATCGCGGCGCAAATTC
GGGCGTGGTCTTACAAAAAGGACGCTGTTAGCGAAATGGCGATTTCCGAATTCGCTCGGCATTATGCCTCTCCCGGCGGC
ATGACGGTGGGGTTCGCCTACTACCGGGCGCTTCGCGACGACGCCGCGCTGGCGGCTTCGTTCGAGGGGCGGAGCATAGA
CATGCCAGTCTTGGCTATTGGTGGGCGCCATGGCGTCGGGACAAAGCTCGCTGATGCACTCAGCAAACAGGCGCGTGACC
TTACCGCGGTGATCGCAGAGGACAGCGGCCACTTTGTCGCTGACGAGATGCCGGATTTCTTTTGCGATCAGCTTAAACGG
TTCCTAGTAGCGTAA

Upstream 100 bases:

>100_bases
ATGCATAACCAATAGTTATGGAAACTGTGACACAATAACTGATAGACCGGCATAGCAGGATCGGCAACCCTCGTGTTCAA
CACAGCAACCGAGGGAACCA

Downstream 100 bases:

>100_bases
TATCGATGCCTGATATCTCCGGTTTTCGTAATACGCCCTCAAGGATTTGCAGAGAATCAAATCGATGAACGTGAGGAACT
GACCTTTCCGCTCGCTAACC

Product: alpha/beta hydrolase fold protein

Products: NA

Alternate protein names: SEH; Cytosolic epoxide hydrolase; cEH; Epoxide hydratase [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MRTMKAERRAFLIGTSGIIFGPALAMMCAPGAMAQSMALRKAPDATLPKGGTSQFVQINGIRLHYVSTGSGPAVILLHGW
PQTWFAWRATMERLSSHFTVIAPDLRGVGLSERTPTGYDKRTIASDIAALIGHAAGGRAHVVAHDMGGKVAYMLANLHPE
SVEKLVLVDCLIPGTENMDALRGGAWHYGFHMAPEIPEMLTKGRERDYIAAQIRAWSYKKDAVSEMAISEFARHYASPGG
MTVGFAYYRALRDDAALAASFEGRSIDMPVLAIGGRHGVGTKLADALSKQARDLTAVIAEDSGHFVADEMPDFFCDQLKR
FLVA

Sequences:

>Translated_324_residues
MRTMKAERRAFLIGTSGIIFGPALAMMCAPGAMAQSMALRKAPDATLPKGGTSQFVQINGIRLHYVSTGSGPAVILLHGW
PQTWFAWRATMERLSSHFTVIAPDLRGVGLSERTPTGYDKRTIASDIAALIGHAAGGRAHVVAHDMGGKVAYMLANLHPE
SVEKLVLVDCLIPGTENMDALRGGAWHYGFHMAPEIPEMLTKGRERDYIAAQIRAWSYKKDAVSEMAISEFARHYASPGG
MTVGFAYYRALRDDAALAASFEGRSIDMPVLAIGGRHGVGTKLADALSKQARDLTAVIAEDSGHFVADEMPDFFCDQLKR
FLVA
>Mature_324_residues
MRTMKAERRAFLIGTSGIIFGPALAMMCAPGAMAQSMALRKAPDATLPKGGTSQFVQINGIRLHYVSTGSGPAVILLHGW
PQTWFAWRATMERLSSHFTVIAPDLRGVGLSERTPTGYDKRTIASDIAALIGHAAGGRAHVVAHDMGGKVAYMLANLHPE
SVEKLVLVDCLIPGTENMDALRGGAWHYGFHMAPEIPEMLTKGRERDYIAAQIRAWSYKKDAVSEMAISEFARHYASPGG
MTVGFAYYRALRDDAALAASFEGRSIDMPVLAIGGRHGVGTKLADALSKQARDLTAVIAEDSGHFVADEMPDFFCDQLKR
FLVA

Specific function: Involved in catabolic degradation of epoxides. Shows highest activity towards C6 and C7 carbocyclic epoxides. Also active towards linear 1,2-epoxyalkanes [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Epoxide hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI218777840, Length=283, Percent_Identity=28.2685512367491, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI13376164, Length=283, Percent_Identity=28.2685512367491, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI218777837, Length=303, Percent_Identity=23.7623762376238, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI27597073, Length=305, Percent_Identity=22.9508196721311, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17554012, Length=303, Percent_Identity=27.3927392739274, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17562298, Length=298, Percent_Identity=25.8389261744966, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24646055, Length=314, Percent_Identity=26.1146496815287, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639
- InterPro:   IPR010497 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF06441 EHN [H]

EC number: =3.3.2.10 [H]

Molecular weight: Translated: 35050; Mature: 35050

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTMKAERRAFLIGTSGIIFGPALAMMCAPGAMAQSMALRKAPDATLPKGGTSQFVQING
CCCCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEC
IRLHYVSTGSGPAVILLHGWPQTWFAWRATMERLSSHFTVIAPDLRGVGLSERTPTGYDK
EEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCH
RTIASDIAALIGHAAGGRAHVVAHDMGGKVAYMLANLHPESVEKLVLVDCLIPGTENMDA
HHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEECCCHHHHHHEEEEEEECCCCCCHHH
LRGGAWHYGFHMAPEIPEMLTKGRERDYIAAQIRAWSYKKDAVSEMAISEFARHYASPGG
HCCCCEECCCCCCCCHHHHHHCCCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHHHCCCCC
MTVGFAYYRALRDDAALAASFEGRSIDMPVLAIGGRHGVGTKLADALSKQARDLTAVIAE
CCHHHHHHHHHHHCHHHEEECCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHEEEEEEC
DSGHFVADEMPDFFCDQLKRFLVA
CCCCEEHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRTMKAERRAFLIGTSGIIFGPALAMMCAPGAMAQSMALRKAPDATLPKGGTSQFVQING
CCCCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEC
IRLHYVSTGSGPAVILLHGWPQTWFAWRATMERLSSHFTVIAPDLRGVGLSERTPTGYDK
EEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCH
RTIASDIAALIGHAAGGRAHVVAHDMGGKVAYMLANLHPESVEKLVLVDCLIPGTENMDA
HHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEECCCHHHHHHEEEEEEECCCCCCHHH
LRGGAWHYGFHMAPEIPEMLTKGRERDYIAAQIRAWSYKKDAVSEMAISEFARHYASPGG
HCCCCEECCCCCCCCHHHHHHCCCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHHHCCCCC
MTVGFAYYRALRDDAALAASFEGRSIDMPVLAIGGRHGVGTKLADALSKQARDLTAVIAE
CCHHHHHHHHHHHCHHHEEECCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHEEEEEEC
DSGHFVADEMPDFFCDQLKRFLVA
CCCCEEHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9578475 [H]