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The map label for this gene is cysG [H]

Identifier: 182677265

GI number: 182677265

Start: 306960

End: 308525

Strand: Direct

Name: cysG [H]

Synonym: Bind_0267

Alternate gene names: 182677265

Gene position: 306960-308525 (Clockwise)

Preceding gene: 182677263

Following gene: 182677267

Centisome position: 7.36

GC content: 62.96

Gene sequence:

>1566_bases
ATGGTTGATCTCCTCGCACGGACAGATTCGCGTGCCGAGCCCGAAATCGAGGCTGCCCCCTCAGCACCGGCTGCATCCTC
ATCGGCCGAAGGACTGAGTTGCCTGCCGCTTTTCTTTCCTCTTGCCGGGCGAACCGTCGTCCTCGCCGGTGATGGGGAGG
CCCTGGTCTGGAAAGCTGAGCTTTTGTACTCGACCGGGGCTTATCTGCGTGTTTTCGCGCCGCAACCCGTGCCTCGGCTA
GCCGCTTTTCTCCAAAGTGAAGATCACGAGGCCGAAAAGCCCGCCCCATCAGCCTCTTCGCAAGAGCAGCGTTGCCTATG
GCATCAGCGTCCAATCCGGGCGGAGGATCTTTCCGGCGCGGCACTGGTCATTGCCGCCTGCGATGAGGATAGCGAGGCTG
CGGCCCTCCTGGCCATGGCGCGCGCGGCCGGTGTGCCGATCAATATCATCGACAGACCGGAACGGTCGGATTTTCAGTTC
GGCGCGATCCTCGATCGCTCTCCCCTTGTCATCGGCATTTCAACACACGGCGCCGCGCCGGCCCTGGCGCAAGCCCTGCG
CGGCCGGCTCGAAGCCTGGCTGCCTGCTTCTTTGCAACATTGGGCGGCAGCCGCGCGAAACTGGCGCGCACGGTTGAAAC
GGGAGCCGGTGAACAATCCGCGGCGATTTTGGAGTCTGTTCAGCCAGGCGGCGCTTGCGGCGGATCGCGAGGCGCCGCGC
GAAACGGATTTCCAGCGCATGCGCGAGGAGAGCCAGAAACAGGACGATGCAGAATCCCTGGAGGAAACCATAAGCCCCAA
CCAAAAAGGTGTCGTCGCTCTGGTCGGTGCGGGACCAGGTGATCCCGAACTCCTGACCTTGAAGGCGCTGCGCTTCCTAC
AGGAAGCGGACGTCGTCCTCTATGACGATCTCGTTTCGCCACGCATCGTCGCCATGGCACGGCGCGATGCGGAAAAGATC
CCGGTCGGCAAGCGCGGCTATCGTCCCTCATGCCGGCAGGATCATATTACCGCGCAATTGGTCGCGCTGGGGTTGGCGGG
CAAGAAAGTGGTTCGTCTCAAGGGAGGTGACCCGATGATTTTCGGCCGCGCCAATGAGGAAATCGCGGCCCTGCGCGCGG
TGGGCGTCAGGGTTGAGGTCGTTCCTGGCATCACGGCGGCGCTCGGCGCGGCCGCGAGCTTGCAAATGTCATTGACCGAG
CGCGACAAAGCGCGGCGCCTGCAATTCATCACTGCCCATGCGCATGATGGAAGCTTGCCGGACGACATGGACTGGCGGGC
TCTTTGTGATCCGCGCGCTTCAAGTATCGTTTATATGGGGGTGAAAACCCTGCAGGCTTTGGCCTCGCGGCTCCTCGCAG
AGGGGATCGATCCGACGACGCCGGCTTTGCTCATCGAACGCGCGACCCAACGGGACGAAAGGGTACTCGCCGGCACGATC
GAAAGCTTGCCGCCCCTGGTCGCGGCGGCGGCTCCTGACGGCCCCTGTCTCGTTTTCATTGGCGCAGCCTTCGCATCGCC
TGAAACTGAGACATCAAAGGACAGCGTTATCGAAGCCGCCCTGTGA

Upstream 100 bases:

>100_bases
CGCTTTTTCAAAGCGAGAGCGAAAATTCAAGAGCGGTCCAACGGTCATTGATCATGACCGTTGGTATGACATAAGGGTGT
CGATGTGAGGGCAAGGCGCC

Downstream 100 bases:

>100_bases
ATTTTTTTTCACTGAACCCGTGAACTTGCGCCTTCAAAGCAAGGTCCACGCGCCTTCGCGATGGGGTTTCGCGGAGGCAA
AGCCTCAGGCTTCCAGCGAG

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase [H]

Number of amino acids: Translated: 521; Mature: 521

Protein sequence:

>521_residues
MVDLLARTDSRAEPEIEAAPSAPAASSSAEGLSCLPLFFPLAGRTVVLAGDGEALVWKAELLYSTGAYLRVFAPQPVPRL
AAFLQSEDHEAEKPAPSASSQEQRCLWHQRPIRAEDLSGAALVIAACDEDSEAAALLAMARAAGVPINIIDRPERSDFQF
GAILDRSPLVIGISTHGAAPALAQALRGRLEAWLPASLQHWAAAARNWRARLKREPVNNPRRFWSLFSQAALAADREAPR
ETDFQRMREESQKQDDAESLEETISPNQKGVVALVGAGPGDPELLTLKALRFLQEADVVLYDDLVSPRIVAMARRDAEKI
PVGKRGYRPSCRQDHITAQLVALGLAGKKVVRLKGGDPMIFGRANEEIAALRAVGVRVEVVPGITAALGAAASLQMSLTE
RDKARRLQFITAHAHDGSLPDDMDWRALCDPRASSIVYMGVKTLQALASRLLAEGIDPTTPALLIERATQRDERVLAGTI
ESLPPLVAAAAPDGPCLVFIGAAFASPETETSKDSVIEAAL

Sequences:

>Translated_521_residues
MVDLLARTDSRAEPEIEAAPSAPAASSSAEGLSCLPLFFPLAGRTVVLAGDGEALVWKAELLYSTGAYLRVFAPQPVPRL
AAFLQSEDHEAEKPAPSASSQEQRCLWHQRPIRAEDLSGAALVIAACDEDSEAAALLAMARAAGVPINIIDRPERSDFQF
GAILDRSPLVIGISTHGAAPALAQALRGRLEAWLPASLQHWAAAARNWRARLKREPVNNPRRFWSLFSQAALAADREAPR
ETDFQRMREESQKQDDAESLEETISPNQKGVVALVGAGPGDPELLTLKALRFLQEADVVLYDDLVSPRIVAMARRDAEKI
PVGKRGYRPSCRQDHITAQLVALGLAGKKVVRLKGGDPMIFGRANEEIAALRAVGVRVEVVPGITAALGAAASLQMSLTE
RDKARRLQFITAHAHDGSLPDDMDWRALCDPRASSIVYMGVKTLQALASRLLAEGIDPTTPALLIERATQRDERVLAGTI
ESLPPLVAAAAPDGPCLVFIGAAFASPETETSKDSVIEAAL
>Mature_521_residues
MVDLLARTDSRAEPEIEAAPSAPAASSSAEGLSCLPLFFPLAGRTVVLAGDGEALVWKAELLYSTGAYLRVFAPQPVPRL
AAFLQSEDHEAEKPAPSASSQEQRCLWHQRPIRAEDLSGAALVIAACDEDSEAAALLAMARAAGVPINIIDRPERSDFQF
GAILDRSPLVIGISTHGAAPALAQALRGRLEAWLPASLQHWAAAARNWRARLKREPVNNPRRFWSLFSQAALAADREAPR
ETDFQRMREESQKQDDAESLEETISPNQKGVVALVGAGPGDPELLTLKALRFLQEADVVLYDDLVSPRIVAMARRDAEKI
PVGKRGYRPSCRQDHITAQLVALGLAGKKVVRLKGGDPMIFGRANEEIAALRAVGVRVEVVPGITAALGAAASLQMSLTE
RDKARRLQFITAHAHDGSLPDDMDWRALCDPRASSIVYMGVKTLQALASRLLAEGIDPTTPALLIERATQRDERVLAGTI
ESLPPLVAAAAPDGPCLVFIGAAFASPETETSKDSVIEAAL

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=472, Percent_Identity=35.3813559322034, Blast_Score=244, Evalue=7e-66,
Organism=Saccharomyces cerevisiae, GI6322922, Length=256, Percent_Identity=30.46875, Blast_Score=105, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043 [H]

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4 [H]

Molecular weight: Translated: 55908; Mature: 55908

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDLLARTDSRAEPEIEAAPSAPAASSSAEGLSCLPLFFPLAGRTVVLAGDGEALVWKAE
CCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHCCCCCEEEEECCCCEEEEEEE
LLYSTGAYLRVFAPQPVPRLAAFLQSEDHEAEKPAPSASSQEQRCLWHQRPIRAEDLSGA
HHHCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCC
ALVIAACDEDSEAAALLAMARAAGVPINIIDRPERSDFQFGAILDRSPLVIGISTHGAAP
EEEEEECCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEECCCCEEEEEECCCCCH
ALAQALRGRLEAWLPASLQHWAAAARNWRARLKREPVNNPRRFWSLFSQAALAADREAPR
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCC
ETDFQRMREESQKQDDAESLEETISPNQKGVVALVGAGPGDPELLTLKALRFLQEADVVL
CHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCEEE
YDDLVSPRIVAMARRDAEKIPVGKRGYRPSCRQDHITAQLVALGLAGKKVVRLKGGDPMI
ECCCCCCHHHHHHHHCHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCEEE
FGRANEEIAALRAVGVRVEVVPGITAALGAAASLQMSLTERDKARRLQFITAHAHDGSLP
EECCCHHHHHHHHHCEEEEECCCHHHHHCCHHHEEEHHHHHHHHHEEEEEEEECCCCCCC
DDMDWRALCDPRASSIVYMGVKTLQALASRLLAEGIDPTTPALLIERATQRDERVLAGTI
CCCCCHHHCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
ESLPPLVAAAAPDGPCLVFIGAAFASPETETSKDSVIEAAL
HHCCCCEEECCCCCCEEEEEECHHCCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MVDLLARTDSRAEPEIEAAPSAPAASSSAEGLSCLPLFFPLAGRTVVLAGDGEALVWKAE
CCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHCCCCCEEEEECCCCEEEEEEE
LLYSTGAYLRVFAPQPVPRLAAFLQSEDHEAEKPAPSASSQEQRCLWHQRPIRAEDLSGA
HHHCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCC
ALVIAACDEDSEAAALLAMARAAGVPINIIDRPERSDFQFGAILDRSPLVIGISTHGAAP
EEEEEECCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEECCCCEEEEEECCCCCH
ALAQALRGRLEAWLPASLQHWAAAARNWRARLKREPVNNPRRFWSLFSQAALAADREAPR
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCC
ETDFQRMREESQKQDDAESLEETISPNQKGVVALVGAGPGDPELLTLKALRFLQEADVVL
CHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCEEE
YDDLVSPRIVAMARRDAEKIPVGKRGYRPSCRQDHITAQLVALGLAGKKVVRLKGGDPMI
ECCCCCCHHHHHHHHCHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCEEE
FGRANEEIAALRAVGVRVEVVPGITAALGAAASLQMSLTERDKARRLQFITAHAHDGSLP
EECCCHHHHHHHHHCEEEEECCCHHHHHCCHHHEEEHHHHHHHHHEEEEEEEECCCCCCC
DDMDWRALCDPRASSIVYMGVKTLQALASRLLAEGIDPTTPALLIERATQRDERVLAGTI
CCCCCHHHCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
ESLPPLVAAAAPDGPCLVFIGAAFASPETETSKDSVIEAAL
HHCCCCEEECCCCCCEEEEEECHHCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA