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The map label for this gene is proP [H]

Identifier: 182677176

GI number: 182677176

Start: 209216

End: 210619

Strand: Direct

Name: proP [H]

Synonym: Bind_0176

Alternate gene names: 182677176

Gene position: 209216-210619 (Clockwise)

Preceding gene: 182677175

Following gene: 182677178

Centisome position: 5.02

GC content: 57.48

Gene sequence:

>1404_bases
ATGACGACACAGAGGGCGGACTCCGATGTAGAGAACGGGGCCGGGCTGAAATTCCTGGTTTTTGCATCAAGCCTTGGCAC
CATGATCGAATGGTATGATTTCTTTCTCTACGGCAGCCTGGCGGTTTTCTTTTCTGAACTCTTCTATCCCAAGGACAATC
CGGTTGCGGCTTTGCTCATCAGCGTTGCCACTTTTGCGACGGGCTTTGCCGTGCGTCCTCTTGGTGCCGTATTGTTCGGC
CATCTCGGCGACAGGATCGGTCGCAAGACGACTTTCCTGATCACCTTGCTCTTGATGGGTGGTGCCACGGCCCTGATCGG
CATTTTGCCGACCTATGCCAGCGTCGGCCTTTTGGCGCCTGTCCTGCTCGTCGTGCTGCGTCTCATCCAAGGTCTGGCGC
TCGGTGGCGAATATGGTGGCGCGGCAACTTATGTGGCCGAACATGCGCCAGATGGCAGACGGGGTGGCTGGACGAGCGCG
ATCCAGACCATGGCCTCGATGGGGTTCTTCGTATCTCTCGCGGTGGTCCTGTCCTGCCGTTTTCTTTTGGGTGATGCCAT
CTTTGCATCCTGGGGCTGGCGCATTCCCTTCCTGCTTTCGGCCGTGCTGGTGATGCTCTCTCTTGGGGCGCGTTTACGGC
TTGCCGAATCGCCGATCTTTCTTCAGCTCAAGGCCGAGGATCGTGTGTCGAAATCACCCGTGCGCGAAAGTTTCGCCGAT
CCGCGTAATCGCCGCCTGATGCTCGCCGTTCTGTTCGGAGTCGCGAGCGGTCAGGCCGTGACGTTCTATACGGCAAATTT
TTACGCGCTTTATTTCCTCCAGAGTATTTTGAAGGTGGATTTCCTGACCTCGAATCTTGTGATGGTTCTGGGTGTCTTGA
TCGGCATGCCTCTCTTTGTCCTGTTTGGCATCTGGTCCGATAAAATCGGCCGCAAGCCGCTCATCATCGCCGGTATGGCG
GCGGCGGTCGTGCTGCTAGTGCCGATCTTCATGGTCATGCAGTCGGCGGTCGGTCCGCAGGGTATCAATATCTGGTTGCT
GGCAGCCTGTGTTTTCGTTCTCGTCGCGATTGCGGCGGCGGTCTATGGACCTTACGCGGCCTATCTCGTCGAGGTCTTTC
CGCCGCAGATTCGTTATACGTCCTTGTCGATCCCCTATCATATCGGCAATGGCATTTTCGGCGGCTTTCTGCCGCTGATT
GGCTTGTCACTGGTCGCGGCCACAGGCAATCCGCTCGCCGGGCTCATCTATCCGATTGCGGTTTGCGTGATCAATGTCAT
CATTGGCAGCCTCTATCTGCCGGAGACATTGCATACACCGATTGATGGCCGGACCTCCGTCGAGGATCTGGCGGATGAAA
GCCTGCCTTTCGCGGCCGCGCTGCCGCCGAAATCCGCCGCCTAA

Upstream 100 bases:

>100_bases
TTTGGACGATAAAGGCGCATACGTCGAAAGGGGAAATTTTTAGAAGAGGAACGATGCGTTAGGATAAACAGATAAAGACA
AAATAGTGGGAGGAATTAGC

Downstream 100 bases:

>100_bases
TCGCAGCCTGTAATCTCTTGTGCCAAAGGGCGGAGCCGGCAAGGTTCCCGCCCTTTCTCATTCCCTTTTCTCAACGAGAT
TCCGACGCCGGTCGGAAAAC

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MTTQRADSDVENGAGLKFLVFASSLGTMIEWYDFFLYGSLAVFFSELFYPKDNPVAALLISVATFATGFAVRPLGAVLFG
HLGDRIGRKTTFLITLLLMGGATALIGILPTYASVGLLAPVLLVVLRLIQGLALGGEYGGAATYVAEHAPDGRRGGWTSA
IQTMASMGFFVSLAVVLSCRFLLGDAIFASWGWRIPFLLSAVLVMLSLGARLRLAESPIFLQLKAEDRVSKSPVRESFAD
PRNRRLMLAVLFGVASGQAVTFYTANFYALYFLQSILKVDFLTSNLVMVLGVLIGMPLFVLFGIWSDKIGRKPLIIAGMA
AAVVLLVPIFMVMQSAVGPQGINIWLLAACVFVLVAIAAAVYGPYAAYLVEVFPPQIRYTSLSIPYHIGNGIFGGFLPLI
GLSLVAATGNPLAGLIYPIAVCVINVIIGSLYLPETLHTPIDGRTSVEDLADESLPFAAALPPKSAA

Sequences:

>Translated_467_residues
MTTQRADSDVENGAGLKFLVFASSLGTMIEWYDFFLYGSLAVFFSELFYPKDNPVAALLISVATFATGFAVRPLGAVLFG
HLGDRIGRKTTFLITLLLMGGATALIGILPTYASVGLLAPVLLVVLRLIQGLALGGEYGGAATYVAEHAPDGRRGGWTSA
IQTMASMGFFVSLAVVLSCRFLLGDAIFASWGWRIPFLLSAVLVMLSLGARLRLAESPIFLQLKAEDRVSKSPVRESFAD
PRNRRLMLAVLFGVASGQAVTFYTANFYALYFLQSILKVDFLTSNLVMVLGVLIGMPLFVLFGIWSDKIGRKPLIIAGMA
AAVVLLVPIFMVMQSAVGPQGINIWLLAACVFVLVAIAAAVYGPYAAYLVEVFPPQIRYTSLSIPYHIGNGIFGGFLPLI
GLSLVAATGNPLAGLIYPIAVCVINVIIGSLYLPETLHTPIDGRTSVEDLADESLPFAAALPPKSAA
>Mature_466_residues
TTQRADSDVENGAGLKFLVFASSLGTMIEWYDFFLYGSLAVFFSELFYPKDNPVAALLISVATFATGFAVRPLGAVLFGH
LGDRIGRKTTFLITLLLMGGATALIGILPTYASVGLLAPVLLVVLRLIQGLALGGEYGGAATYVAEHAPDGRRGGWTSAI
QTMASMGFFVSLAVVLSCRFLLGDAIFASWGWRIPFLLSAVLVMLSLGARLRLAESPIFLQLKAEDRVSKSPVRESFADP
RNRRLMLAVLFGVASGQAVTFYTANFYALYFLQSILKVDFLTSNLVMVLGVLIGMPLFVLFGIWSDKIGRKPLIIAGMAA
AVVLLVPIFMVMQSAVGPQGINIWLLAACVFVLVAIAAAVYGPYAAYLVEVFPPQIRYTSLSIPYHIGNGIFGGFLPLIG
LSLVAATGNPLAGLIYPIAVCVINVIIGSLYLPETLHTPIDGRTSVEDLADESLPFAAALPPKSAA

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=449, Percent_Identity=32.739420935412, Blast_Score=221, Evalue=7e-59,
Organism=Escherichia coli, GI1788292, Length=410, Percent_Identity=33.6585365853659, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1788942, Length=405, Percent_Identity=32.5925925925926, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1789941, Length=374, Percent_Identity=36.8983957219251, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI87082404, Length=332, Percent_Identity=25.6024096385542, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87082231, Length=216, Percent_Identity=27.3148148148148, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786229, Length=347, Percent_Identity=25.9365994236311, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 49831; Mature: 49699

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS00024 HEMOPEXIN ; PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQRADSDVENGAGLKFLVFASSLGTMIEWYDFFLYGSLAVFFSELFYPKDNPVAALLI
CCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
SVATFATGFAVRPLGAVLFGHLGDRIGRKTTFLITLLLMGGATALIGILPTYASVGLLAP
HHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
VLLVVLRLIQGLALGGEYGGAATYVAEHAPDGRRGGWTSAIQTMASMGFFVSLAVVLSCR
HHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FLLGDAIFASWGWRIPFLLSAVLVMLSLGARLRLAESPIFLQLKAEDRVSKSPVRESFAD
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCCCCCHHHHHHCC
PRNRRLMLAVLFGVASGQAVTFYTANFYALYFLQSILKVDFLTSNLVMVLGVLIGMPLFV
CCCCEEHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFGIWSDKIGRKPLIIAGMAAAVVLLVPIFMVMQSAVGPQGINIWLLAACVFVLVAIAAA
HHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
VYGPYAAYLVEVFPPQIRYTSLSIPYHIGNGIFGGFLPLIGLSLVAATGNPLAGLIYPIA
HHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
VCVINVIIGSLYLPETLHTPIDGRTSVEDLADESLPFAAALPPKSAA
HHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHCCCCCEECCCCCCCC
>Mature Secondary Structure 
TTQRADSDVENGAGLKFLVFASSLGTMIEWYDFFLYGSLAVFFSELFYPKDNPVAALLI
CCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
SVATFATGFAVRPLGAVLFGHLGDRIGRKTTFLITLLLMGGATALIGILPTYASVGLLAP
HHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
VLLVVLRLIQGLALGGEYGGAATYVAEHAPDGRRGGWTSAIQTMASMGFFVSLAVVLSCR
HHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FLLGDAIFASWGWRIPFLLSAVLVMLSLGARLRLAESPIFLQLKAEDRVSKSPVRESFAD
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCCCCCHHHHHHCC
PRNRRLMLAVLFGVASGQAVTFYTANFYALYFLQSILKVDFLTSNLVMVLGVLIGMPLFV
CCCCEEHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFGIWSDKIGRKPLIIAGMAAAVVLLVPIFMVMQSAVGPQGINIWLLAACVFVLVAIAAA
HHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
VYGPYAAYLVEVFPPQIRYTSLSIPYHIGNGIFGGFLPLIGLSLVAATGNPLAGLIYPIA
HHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
VCVINVIIGSLYLPETLHTPIDGRTSVEDLADESLPFAAALPPKSAA
HHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHCCCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]