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The map label for this gene is argF [H]

Identifier: 182677152

GI number: 182677152

Start: 181115

End: 182074

Strand: Direct

Name: argF [H]

Synonym: Bind_0151

Alternate gene names: 182677152

Gene position: 181115-182074 (Clockwise)

Preceding gene: 182677151

Following gene: 182677153

Centisome position: 4.34

GC content: 60.52

Gene sequence:

>960_bases
ATGACGCCTCTCCCTGTGTCCGATCTCATCGTGCAGCCCAAACATTTTCTTGATTTGTCCCGGCTTGAAGCCAAGGATCT
CCGCGCTATTCTCGATTTGGCGGCGGGTTTGAAGGCCAAGCGCCGGCGTGGCCAAAAAGCCAAGGAGCAGCCGCTCGAGG
GCAAGGTCCTGGCGATGGTGTTTGACCAGCCCTCGACCCGCACCCGCGTTTCCTTCGATCTCGCCATGCGTGAGCTGGGC
GGCGAGACCATCATGCTGACCGGTCAGGAAATGCAGCTCGGGCGCGGCGAGACCATCGCCGATACGGCGCGCGTTCTCTC
GCGTTTCGTCGATGCGATCGTCATCCGCATCCTCAATCATGCCGATCTCCTGGAACTCGCCGAATATGCGGAAGTGCCGG
TTATCAATGGCTTGACGAAACTCTCGCACCCTTGCCAGATCATGGCCGATCTCCTCACATTCGAGGAGCATCGCGGGCCG
ATCAAGGGCCGCACCATCGCCTGGACCGGTGATTCCAACAATGTGCTCGCAAGCTGGATCGAGGCCGCGCGGCGGCTCGA
TTTCACCTTGAACGTCGCCTGTCCGGCCGAGCTTTCACCCGCGCCCGAGCTCGTCGCTTTTGCCAATGCTCAGGGCAAGA
GGCTGAATGTTCTGCGCGACCCCTTCGAGGCGGTGCGCGGCGCCGATGCGGTGATCTCCGATTGCTGGGTATCGATGGGC
GATGAGGAAGAGGAAGCCCGCCGTCAGGCCTTGCTCTCGCCCTATCAGGTCAATGCCGAGCTGATGGCGACTGCAGCCAA
GGACGCGATCTTCATGCATTGCCTGCCGGCGCATCGTGGTGAGGAAGTCACCGATGAGGTGATCGATGGGCCGCAATCGG
TGGTCTTCGATGAAGCTGAAAACCGCCTGCATGCGCAAAAAGGCATTCTCGCCTGGTGTTTTGGAGTAACCGGTTCATGA

Upstream 100 bases:

>100_bases
AGGAAGTGGATGGCGCGATCGAGCGTTTGGACGCGGCTTGCGCGGCGCTCAAAGCCCGAATCAACGCCAATAGCTCCTCT
CTCCTGAGCGGAGCCGCTGA

Downstream 100 bases:

>100_bases
ATGAATCCGCCGCGCGCTTCGTCTCCGATAATGGCGAGGATGACAGCGTGCTGCCTTTCGCCGTGGAGCCGCTCGACGTG
CGCGGCCGCGTGGCAAGGCT

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MTPLPVSDLIVQPKHFLDLSRLEAKDLRAILDLAAGLKAKRRRGQKAKEQPLEGKVLAMVFDQPSTRTRVSFDLAMRELG
GETIMLTGQEMQLGRGETIADTARVLSRFVDAIVIRILNHADLLELAEYAEVPVINGLTKLSHPCQIMADLLTFEEHRGP
IKGRTIAWTGDSNNVLASWIEAARRLDFTLNVACPAELSPAPELVAFANAQGKRLNVLRDPFEAVRGADAVISDCWVSMG
DEEEEARRQALLSPYQVNAELMATAAKDAIFMHCLPAHRGEEVTDEVIDGPQSVVFDEAENRLHAQKGILAWCFGVTGS

Sequences:

>Translated_319_residues
MTPLPVSDLIVQPKHFLDLSRLEAKDLRAILDLAAGLKAKRRRGQKAKEQPLEGKVLAMVFDQPSTRTRVSFDLAMRELG
GETIMLTGQEMQLGRGETIADTARVLSRFVDAIVIRILNHADLLELAEYAEVPVINGLTKLSHPCQIMADLLTFEEHRGP
IKGRTIAWTGDSNNVLASWIEAARRLDFTLNVACPAELSPAPELVAFANAQGKRLNVLRDPFEAVRGADAVISDCWVSMG
DEEEEARRQALLSPYQVNAELMATAAKDAIFMHCLPAHRGEEVTDEVIDGPQSVVFDEAENRLHAQKGILAWCFGVTGS
>Mature_318_residues
TPLPVSDLIVQPKHFLDLSRLEAKDLRAILDLAAGLKAKRRRGQKAKEQPLEGKVLAMVFDQPSTRTRVSFDLAMRELGG
ETIMLTGQEMQLGRGETIADTARVLSRFVDAIVIRILNHADLLELAEYAEVPVINGLTKLSHPCQIMADLLTFEEHRGPI
KGRTIAWTGDSNNVLASWIEAARRLDFTLNVACPAELSPAPELVAFANAQGKRLNVLRDPFEAVRGADAVISDCWVSMGD
EEEEARRQALLSPYQVNAELMATAAKDAIFMHCLPAHRGEEVTDEVIDGPQSVVFDEAENRLHAQKGILAWCFGVTGS

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=297, Percent_Identity=41.0774410774411, Blast_Score=231, Evalue=9e-61,
Organism=Homo sapiens, GI18105007, Length=313, Percent_Identity=27.4760383386581, Blast_Score=103, Evalue=1e-22,
Organism=Escherichia coli, GI1790703, Length=325, Percent_Identity=40.6153846153846, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1786469, Length=338, Percent_Identity=39.9408284023669, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI2367364, Length=312, Percent_Identity=28.2051282051282, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI48994908, Length=345, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI193204318, Length=331, Percent_Identity=28.0966767371601, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6322373, Length=309, Percent_Identity=31.0679611650485, Blast_Score=170, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6322331, Length=312, Percent_Identity=27.8846153846154, Blast_Score=101, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24642586, Length=311, Percent_Identity=28.2958199356913, Blast_Score=108, Evalue=6e-24,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 35102; Mature: 34971

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPLPVSDLIVQPKHFLDLSRLEAKDLRAILDLAAGLKAKRRRGQKAKEQPLEGKVLAMV
CCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEE
FDQPSTRTRVSFDLAMRELGGETIMLTGQEMQLGRGETIADTARVLSRFVDAIVIRILNH
ECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
ADLLELAEYAEVPVINGLTKLSHPCQIMADLLTFEEHRGPIKGRTIAWTGDSNNVLASWI
HHHHHHHHHHCCCHHCCHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHH
EAARRLDFTLNVACPAELSPAPELVAFANAQGKRLNVLRDPFEAVRGADAVISDCWVSMG
HHHHHCCEEEEEECCCCCCCCCHHEEECCCCCCCHHHHHCHHHHHCCHHHHHHHHHHCCC
DEEEEARRQALLSPYQVNAELMATAAKDAIFMHCLPAHRGEEVTDEVIDGPQSVVFDEAE
CCHHHHHHHHHCCCHHCCHHHHHHHHHHHHHEEECCCCCCCHHHHHHHCCCHHHHHHHHH
NRLHAQKGILAWCFGVTGS
HHHHHHHCCEEEEECCCCC
>Mature Secondary Structure 
TPLPVSDLIVQPKHFLDLSRLEAKDLRAILDLAAGLKAKRRRGQKAKEQPLEGKVLAMV
CCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEE
FDQPSTRTRVSFDLAMRELGGETIMLTGQEMQLGRGETIADTARVLSRFVDAIVIRILNH
ECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
ADLLELAEYAEVPVINGLTKLSHPCQIMADLLTFEEHRGPIKGRTIAWTGDSNNVLASWI
HHHHHHHHHHCCCHHCCHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHH
EAARRLDFTLNVACPAELSPAPELVAFANAQGKRLNVLRDPFEAVRGADAVISDCWVSMG
HHHHHCCEEEEEECCCCCCCCCHHEEECCCCCCCHHHHHCHHHHHCCHHHHHHHHHHCCC
DEEEEARRQALLSPYQVNAELMATAAKDAIFMHCLPAHRGEEVTDEVIDGPQSVVFDEAE
CCHHHHHHHHHCCCHHCCHHHHHHHHHHHHHEEECCCCCCCHHHHHHHCCCHHHHHHHHH
NRLHAQKGILAWCFGVTGS
HHHHHHHCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA