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The map label for this gene is pntB [H]

Identifier: 17547452

GI number: 17547452

Start: 2940154

End: 2941614

Strand: Direct

Name: pntB [H]

Synonym: RSc2733

Alternate gene names: 17547452

Gene position: 2940154-2941614 (Clockwise)

Preceding gene: 17547451

Following gene: 17547453

Centisome position: 79.11

GC content: 66.32

Gene sequence:

>1461_bases
ATGAGCATGAACCTCGTCACCCTGCTGTACCTGCTGGCGTCGGTCTGCTTCATCCAGGCGCTCAAGGGCCTGTCGCACCC
GGGCACTGCGCGACGCGGCAATGTGTTCGGCATGAGCGGCATGGCGATCGCGGCGGTCACCACCATCGCGCTCATCTACA
AGCTCAAGGCCGAGATGTTCGCCGACACCGAGAGCGGCACGGCCACGGGCTTTCTGCTGATCTTCGCCGGGCTGGTGGCG
GGCGGCGGCATCGGCGCCTACGTCGCGCGCACGGTCGAGATGACCAAGATGCCCGAACTGGTGGCGGCCATGCACTCGCT
GATCGGCCTGGCGGCGGTCTGCATCGCCGTCGCGGCGGTGGCCGAGCCCGCGGCCTTCGGCATCACGCTGGCCGGCGACA
ACGTGCTGCCGCTGGGCAACCGCGTCGAGCTGTTCATCGGCACCTTCGTCGGCGCCATCACCTTCTCCGGTTCGGTGATC
GCCTTCGGCAAGCTGTCGGGCAAATACAAATTCCGCCTGTTCCAGGGCGCGCCGGTGCAGTTCGCCGGCCAGCACATGCT
GAACCTGCTGCTGGCGCTGGCCATGCTGGGCTTCGGCGTGCTGTTCTTCCTGTCCCAGAGCTGGCTGCCGTTCGTCATCA
TGACGGCCATCGCCTTCGCACTGGGCGTGCTGATCATCATCCCCATCGGCGGCGCCGACATGCCGGTGGTGGTGTCGATG
CTGAATTCCTACTCGGGCTGGGCCGCGGCGGGCATCGGCTTCTCGCTGAACAACCCGATGCTGATCATCGCCGGCTCGCT
GGTCGGCTCTTCCGGCGCGATCCTGTCGTACATCATGTGCCGGGCGATGAACCGGTCGTTCTTCAACGTGCTGCTGGGCG
GCTTCGGCTCGGAAGCGTCGGCCGGGGCCGCAGCAAGTGGCGACGGCCAGCAGCGCGCCGTCAAGTCGGGCTCGCCGGAC
GACGCCGCCTTCCTGCTGGGCAACGCCGAGACCGTCATCATCGTGCCCGGCTACGGCCTGGCGGTGGCGCGCGCGCAGCA
CGCCCTCAAGGAGCTGACCGAGCTGCTGGCCGAGAAGGGCGTGACGGTCAAGTACGCCATCCATCCCGTCGCCGGCCGCA
TGCCGGGCCACATGAACGTGCTGCTGGCCGAGGCCGAGGTGCCGTACGACCAGGTCTTCGAGATGGAAGACATCAACAGC
GAGTTCGGCCAGGCCGACGTGGTGCTGGTGCTGGGCGCCAACGACGTGGTCAACCCGGCCGCCAAGAACGACCCGAAATC
CCCCATTGCCGGCATGCCGATCCTGGAGGCCTACAAGGCCAAGACCATCATCGTCAACAAGCGCTCGATGAACGCCGGAT
ACGCCGGGCTGGACAACGAGCTCTTCTACATGGACAAGACCATGATGGTGTTCGGCGATGCCAAGAAGGTGGTCGAAGAC
ATGGTCAAGGCGGTGGACTGA

Upstream 100 bases:

>100_bases
CGGCGGTCAACGTGTTCGGCGGCTTCCTGGTCACGCAGCGCATGCTCGAGATGTTCAAGAAGAAGGCGCCCAAGGCCGGC
AAGAACGGGGAGGCCCAGTC

Downstream 100 bases:

>100_bases
GCGCCGGCCCGCGTTCGCCCATCGCTGACGGGCGTCAATTCCCATGCGGGACGCGACGCAATCCGCTTGCCCCCGGCGTC
GGCAGGCAGTAACTTAGTGC

Product: transmembrane NADP transhydrogenase subunit beta oxidoreductase

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit beta; Proton-translocating transhydrogenase NADP(H)-binding component; Pyridine nucleotide transhydrogenase subunit beta; dIII [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MSMNLVTLLYLLASVCFIQALKGLSHPGTARRGNVFGMSGMAIAAVTTIALIYKLKAEMFADTESGTATGFLLIFAGLVA
GGGIGAYVARTVEMTKMPELVAAMHSLIGLAAVCIAVAAVAEPAAFGITLAGDNVLPLGNRVELFIGTFVGAITFSGSVI
AFGKLSGKYKFRLFQGAPVQFAGQHMLNLLLALAMLGFGVLFFLSQSWLPFVIMTAIAFALGVLIIIPIGGADMPVVVSM
LNSYSGWAAAGIGFSLNNPMLIIAGSLVGSSGAILSYIMCRAMNRSFFNVLLGGFGSEASAGAAASGDGQQRAVKSGSPD
DAAFLLGNAETVIIVPGYGLAVARAQHALKELTELLAEKGVTVKYAIHPVAGRMPGHMNVLLAEAEVPYDQVFEMEDINS
EFGQADVVLVLGANDVVNPAAKNDPKSPIAGMPILEAYKAKTIIVNKRSMNAGYAGLDNELFYMDKTMMVFGDAKKVVED
MVKAVD

Sequences:

>Translated_486_residues
MSMNLVTLLYLLASVCFIQALKGLSHPGTARRGNVFGMSGMAIAAVTTIALIYKLKAEMFADTESGTATGFLLIFAGLVA
GGGIGAYVARTVEMTKMPELVAAMHSLIGLAAVCIAVAAVAEPAAFGITLAGDNVLPLGNRVELFIGTFVGAITFSGSVI
AFGKLSGKYKFRLFQGAPVQFAGQHMLNLLLALAMLGFGVLFFLSQSWLPFVIMTAIAFALGVLIIIPIGGADMPVVVSM
LNSYSGWAAAGIGFSLNNPMLIIAGSLVGSSGAILSYIMCRAMNRSFFNVLLGGFGSEASAGAAASGDGQQRAVKSGSPD
DAAFLLGNAETVIIVPGYGLAVARAQHALKELTELLAEKGVTVKYAIHPVAGRMPGHMNVLLAEAEVPYDQVFEMEDINS
EFGQADVVLVLGANDVVNPAAKNDPKSPIAGMPILEAYKAKTIIVNKRSMNAGYAGLDNELFYMDKTMMVFGDAKKVVED
MVKAVD
>Mature_485_residues
SMNLVTLLYLLASVCFIQALKGLSHPGTARRGNVFGMSGMAIAAVTTIALIYKLKAEMFADTESGTATGFLLIFAGLVAG
GGIGAYVARTVEMTKMPELVAAMHSLIGLAAVCIAVAAVAEPAAFGITLAGDNVLPLGNRVELFIGTFVGAITFSGSVIA
FGKLSGKYKFRLFQGAPVQFAGQHMLNLLLALAMLGFGVLFFLSQSWLPFVIMTAIAFALGVLIIIPIGGADMPVVVSML
NSYSGWAAAGIGFSLNNPMLIIAGSLVGSSGAILSYIMCRAMNRSFFNVLLGGFGSEASAGAAASGDGQQRAVKSGSPDD
AAFLLGNAETVIIVPGYGLAVARAQHALKELTELLAEKGVTVKYAIHPVAGRMPGHMNVLLAEAEVPYDQVFEMEDINSE
FGQADVVLVLGANDVVNPAAKNDPKSPIAGMPILEAYKAKTIIVNKRSMNAGYAGLDNELFYMDKTMMVFGDAKKVVEDM
VKAVD

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG1282

COG function: function code C; NAD/NADP transhydrogenase beta subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNT beta subunit family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=485, Percent_Identity=45.1546391752577, Blast_Score=387, Evalue=1e-107,
Organism=Homo sapiens, GI122939153, Length=485, Percent_Identity=45.1546391752577, Blast_Score=387, Evalue=1e-107,
Organism=Escherichia coli, GI1787886, Length=489, Percent_Identity=50.3067484662577, Blast_Score=450, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI17550456, Length=488, Percent_Identity=42.4180327868852, Blast_Score=349, Evalue=1e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012136
- InterPro:   IPR004003 [H]

Pfam domain/function: PF02233 PNTB [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 50732; Mature: 50601

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMNLVTLLYLLASVCFIQALKGLSHPGTARRGNVFGMSGMAIAAVTTIALIYKLKAEMF
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHH
ADTESGTATGFLLIFAGLVAGGGIGAYVARTVEMTKMPELVAAMHSLIGLAAVCIAVAAV
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEPAAFGITLAGDNVLPLGNRVELFIGTFVGAITFSGSVIAFGKLSGKYKFRLFQGAPVQ
HCCHHEEEEEECCCEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEEECCCCH
FAGQHMLNLLLALAMLGFGVLFFLSQSWLPFVIMTAIAFALGVLIIIPIGGADMPVVVSM
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHH
LNSYSGWAAAGIGFSLNNPMLIIAGSLVGSSGAILSYIMCRAMNRSFFNVLLGGFGSEAS
HHCCCCCEEECCCEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
AGAAASGDGQQRAVKSGSPDDAAFLLGNAETVIIVPGYGLAVARAQHALKELTELLAEKG
CCCCCCCCCHHHHHCCCCCCCCEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCC
VTVKYAIHPVAGRMPGHMNVLLAEAEVPYDQVFEMEDINSEFGQADVVLVLGANDVVNPA
CEEEEEEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCC
AKNDPKSPIAGMPILEAYKAKTIIVNKRSMNAGYAGLDNELFYMDKTMMVFGDAKKVVED
CCCCCCCCCCCCCHHHHHCCEEEEEECCCCCCCCCCCCCCEEEECCEEEEECCHHHHHHH
MVKAVD
HHHHCC
>Mature Secondary Structure 
SMNLVTLLYLLASVCFIQALKGLSHPGTARRGNVFGMSGMAIAAVTTIALIYKLKAEMF
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHH
ADTESGTATGFLLIFAGLVAGGGIGAYVARTVEMTKMPELVAAMHSLIGLAAVCIAVAAV
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEPAAFGITLAGDNVLPLGNRVELFIGTFVGAITFSGSVIAFGKLSGKYKFRLFQGAPVQ
HCCHHEEEEEECCCEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEEECCCCH
FAGQHMLNLLLALAMLGFGVLFFLSQSWLPFVIMTAIAFALGVLIIIPIGGADMPVVVSM
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHH
LNSYSGWAAAGIGFSLNNPMLIIAGSLVGSSGAILSYIMCRAMNRSFFNVLLGGFGSEAS
HHCCCCCEEECCCEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
AGAAASGDGQQRAVKSGSPDDAAFLLGNAETVIIVPGYGLAVARAQHALKELTELLAEKG
CCCCCCCCCHHHHHCCCCCCCCEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCC
VTVKYAIHPVAGRMPGHMNVLLAEAEVPYDQVFEMEDINSEFGQADVVLVLGANDVVNPA
CEEEEEEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCC
AKNDPKSPIAGMPILEAYKAKTIIVNKRSMNAGYAGLDNELFYMDKTMMVFGDAKKVVED
CCCCCCCCCCCCCHHHHHCCEEEEEECCCCCCCCCCCCCCEEEECCEEEEECCHHHHHHH
MVKAVD
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8075801 [H]