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The map label for this gene is yafV [C]

Identifier: 17547377

GI number: 17547377

Start: 2867018

End: 2867887

Strand: Direct

Name: yafV [C]

Synonym: RSc2658

Alternate gene names: 17547377

Gene position: 2867018-2867887 (Clockwise)

Preceding gene: 17547376

Following gene: 17547378

Centisome position: 77.14

GC content: 71.49

Gene sequence:

>870_bases
ATGAGCACGACCACCCTCGCCCCTGCCGCCGCCGCGTCCGTTCCGGATGCGCCGTTCGATGCACCGTTCCGCGTCGCCGC
CATCCAGACCGTGACGGGCATCGACGTGGACGCCAACCTGGCCCGCGCCGACGCGCTGCTCGCCGAAGCCGCGGCCCGGG
GTGCGCAGCTGGCGCTGCTGCCCGAGTATTTCTGCATGATGGGCCGCAAGGACAGCGACAAGGTCGCCATCCGCGAGGCG
GACCAGGACGGCCCGATCCAGGCCTTCCTGGCCGATGCCGCGCGCCGGCACCGGCTGTGGCTGGTCGGCGGCACGCTGCC
GCTGTGGTGCGAAGACGCCGAGCGCGTGCGCAATACGTCGCTCGCATTCGATCCGGCCGGCCAGCGCGTGGCCCGCTACG
ACAAGATCCATCTGTTCAACTTCGTGCGCGGCGAAGAGCGCTACGACGAAGCGCGCACCATCGAGCCGGGCGCCACGCCG
GTCGCGTTCGAGGCGCCGTGCGGCCGCGTCGGCATGTCGGTGTGCTACGACCTGCGCTTCCCGGAGCTGTACCGGGCGCT
GTCGGCGCAGGGCAATCTGAACCTGATCCTGATGCCGGCCGCCTTCACCTACGTGACGGGCGCGGCGCACTGGGAAATCC
TGCTGCGCGCCCGCGCCGTGGAGAACCAGTGCTACGTGCTCGCCGCCGCGCAAGGCGGACGCCACGAAAACGGCCGCCGC
ACCTGGGGCCACTCCATGCTGGTCGACCCATGGGGCGAGATCATCGCCTCGGTGCCGGAAGGCGAAGGCGTGGCCATCGG
CGACATGGACCCGGCGCGCCTGGCGCAGGTCCGCCGCGACCTGCCCGCGCTCAAGCACCGCGTGATGTAG

Upstream 100 bases:

>100_bases
CCAAGGCGTTCGCCACCCAATACCGCGTCACCGGCAGCTGGGCCGACCCGAAAATCGAGAAGGCCAGCAGCGGCGGGCAA
ACCCCGGCAAAATAGCCCCC

Downstream 100 bases:

>100_bases
CCCAACCGAATTCCCCGAGGACCCACCATGAACGCAGGCGACATCGCCATCCAGAATCTCTCCGTCGCGCGCCGTGTGCT
GCTCGATCCGTATGGCCTCG

Product: nitrilase

Products: carboxylate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MSTTTLAPAAAASVPDAPFDAPFRVAAIQTVTGIDVDANLARADALLAEAAARGAQLALLPEYFCMMGRKDSDKVAIREA
DQDGPIQAFLADAARRHRLWLVGGTLPLWCEDAERVRNTSLAFDPAGQRVARYDKIHLFNFVRGEERYDEARTIEPGATP
VAFEAPCGRVGMSVCYDLRFPELYRALSAQGNLNLILMPAAFTYVTGAAHWEILLRARAVENQCYVLAAAQGGRHENGRR
TWGHSMLVDPWGEIIASVPEGEGVAIGDMDPARLAQVRRDLPALKHRVM

Sequences:

>Translated_289_residues
MSTTTLAPAAAASVPDAPFDAPFRVAAIQTVTGIDVDANLARADALLAEAAARGAQLALLPEYFCMMGRKDSDKVAIREA
DQDGPIQAFLADAARRHRLWLVGGTLPLWCEDAERVRNTSLAFDPAGQRVARYDKIHLFNFVRGEERYDEARTIEPGATP
VAFEAPCGRVGMSVCYDLRFPELYRALSAQGNLNLILMPAAFTYVTGAAHWEILLRARAVENQCYVLAAAQGGRHENGRR
TWGHSMLVDPWGEIIASVPEGEGVAIGDMDPARLAQVRRDLPALKHRVM
>Mature_288_residues
STTTLAPAAAASVPDAPFDAPFRVAAIQTVTGIDVDANLARADALLAEAAARGAQLALLPEYFCMMGRKDSDKVAIREAD
QDGPIQAFLADAARRHRLWLVGGTLPLWCEDAERVRNTSLAFDPAGQRVARYDKIHLFNFVRGEERYDEARTIEPGATPV
AFEAPCGRVGMSVCYDLRFPELYRALSAQGNLNLILMPAAFTYVTGAAHWEILLRARAVENQCYVLAAAQGGRHENGRRT
WGHSMLVDPWGEIIASVPEGEGVAIGDMDPARLAQVRRDLPALKHRVM

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI297632348, Length=291, Percent_Identity=38.8316151202749, Blast_Score=185, Evalue=5e-47,
Organism=Homo sapiens, GI297632350, Length=271, Percent_Identity=39.8523985239852, Blast_Score=184, Evalue=6e-47,
Organism=Homo sapiens, GI5031947, Length=271, Percent_Identity=39.8523985239852, Blast_Score=184, Evalue=6e-47,
Organism=Homo sapiens, GI9910460, Length=271, Percent_Identity=37.6383763837638, Blast_Score=182, Evalue=3e-46,
Organism=Homo sapiens, GI297632346, Length=196, Percent_Identity=34.1836734693878, Blast_Score=96, Evalue=4e-20,
Organism=Escherichia coli, GI1786412, Length=258, Percent_Identity=31.0077519379845, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17556280, Length=266, Percent_Identity=36.0902255639098, Blast_Score=174, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6323383, Length=273, Percent_Identity=32.967032967033, Blast_Score=158, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6322335, Length=299, Percent_Identity=33.1103678929766, Blast_Score=154, Evalue=1e-38,
Organism=Drosophila melanogaster, GI17933642, Length=276, Percent_Identity=35.8695652173913, Blast_Score=175, Evalue=3e-44,
Organism=Drosophila melanogaster, GI21355835, Length=269, Percent_Identity=33.457249070632, Blast_Score=150, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.5.1

Molecular weight: Translated: 31539; Mature: 31408

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTTLAPAAAASVPDAPFDAPFRVAAIQTVTGIDVDANLARADALLAEAAARGAQLALL
CCCCCCCCHHHCCCCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEC
PEYFCMMGRKDSDKVAIREADQDGPIQAFLADAARRHRLWLVGGTLPLWCEDAERVRNTS
HHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCEEEEEECCCCEEECCHHHHCCCC
LAFDPAGQRVARYDKIHLFNFVRGEERYDEARTIEPGATPVAFEAPCGRVGMSVCYDLRF
EEECCCCHHHHHHHHHEEEHHHCCHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHEECCC
PELYRALSAQGNLNLILMPAAFTYVTGAAHWEILLRARAVENQCYVLAAAQGGRHENGRR
HHHHHHHCCCCCEEEEEECCHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCC
TWGHSMLVDPWGEIIASVPEGEGVAIGDMDPARLAQVRRDLPALKHRVM
CCCCCEEECCHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STTTLAPAAAASVPDAPFDAPFRVAAIQTVTGIDVDANLARADALLAEAAARGAQLALL
CCCCCCCHHHCCCCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEC
PEYFCMMGRKDSDKVAIREADQDGPIQAFLADAARRHRLWLVGGTLPLWCEDAERVRNTS
HHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCEEEEEECCCCEEECCHHHHCCCC
LAFDPAGQRVARYDKIHLFNFVRGEERYDEARTIEPGATPVAFEAPCGRVGMSVCYDLRF
EEECCCCHHHHHHHHHEEEHHHCCHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHEECCC
PELYRALSAQGNLNLILMPAAFTYVTGAAHWEILLRARAVENQCYVLAAAQGGRHENGRR
HHHHHHHCCCCCEEEEEECCHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCC
TWGHSMLVDPWGEIIASVPEGEGVAIGDMDPARLAQVRRDLPALKHRVM
CCCCCEEECCHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nitrile; H2O

Specific reaction: A nitrile + H2O = a carboxylate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]