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The map label for this gene is ppnK

Identifier: 17547369

GI number: 17547369

Start: 2851770

End: 2852678

Strand: Direct

Name: ppnK

Synonym: RSc2650

Alternate gene names: 17547369

Gene position: 2851770-2852678 (Clockwise)

Preceding gene: 17547367

Following gene: 17547370

Centisome position: 76.73

GC content: 68.21

Gene sequence:

>909_bases
GTGACTTCCCGCGCGAACGCCACTTCGCCCTTCAAGACCGTCGCGCTGGTCGGCCGCTATTCGGCCGCCAATATCGCCGC
TCCGCTGCTGGAACTGGCGTCGTGCATCGCCGCGCGCGGCCACGATATCGTCTTCGAACGGGAAACCGCCCTGAACATCG
GGGTCCAGGACTACCCCGCCCTGCCGCCCGACGAGATGGCGCGCCATGCCGACGTGGCCGTGGTGCTGGGCGGCGACGGA
ACGCTGCTCGGCATCGGGCGCCACCTGGCCGGCGCGTCGGTGCCGGTCATCGGGGTCAACCACGGCCGGCTCGGCTTCAT
GACGGACATCCCGTTCGAAGACGTGCACAACGTGCTGCCCGACATGCTGGCCGGCCAGTACGAAGCCGAAACCCGCTCGC
TGCTGCAGGCCCAAGTGGTGCGCGATGACGAGACCATCTTCTCCGCCCTGGCCTTCAACGACGTGGTGGTCAACCGCTCG
GGCTTTTCCGGCATGGTCGAGCTGGCCGTCTCGGTGGACGGCTTCTTCATGTACAACCAGCGCTCGGATGGCCTGATCGT
GTCCACGCCCACCGGGTCGACGGCCTATGCGCTGTCGGCGGGCGGGCCGATCCTGCACCCGGCGCTGTCGGGCCTGGTGC
TGGTGCCGATCGCGCCGCACGCGCTGTCCAACCGGCCCATCGTCATTCCGCACGACGCCGAGGTCGTCATCCAGGTCACC
AGCGGGCGCGACGCCAGCGTCAACTTCGACATGCAGTCGCTCACGTCGCTGCTGCCGGGCGACCGCATCGTGGTGCGCCG
CTCCGAACGCACCGTGCGGCTGCTGCATCCGGTCGGCTACAACTACTACGCCACGCTGCGCAAGAAGCTGCACTGGCACG
AGTACCCGACCGAAGACAACCGGCTGTAA

Upstream 100 bases:

>100_bases
CGCCGGGTGGCGACAACGTTCCACCTTCAAACGCGCGCCGGCCATGGCGACCGTCATATCGATATGGTGTAATGCGCGCA
TGTCGATCTCCCCATCCGCC

Downstream 100 bases:

>100_bases
CGCCAACAACCGATATCGCTCGACGCTCCATGCTGCACAGCCTGACCATCCGCGATTTCGTCATCGTCCATGCCCTGGAC
CTCGACCTGGCCGACGGCTT

Product: NAD(+)/NADH kinase family protein

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MTSRANATSPFKTVALVGRYSAANIAAPLLELASCIAARGHDIVFERETALNIGVQDYPALPPDEMARHADVAVVLGGDG
TLLGIGRHLAGASVPVIGVNHGRLGFMTDIPFEDVHNVLPDMLAGQYEAETRSLLQAQVVRDDETIFSALAFNDVVVNRS
GFSGMVELAVSVDGFFMYNQRSDGLIVSTPTGSTAYALSAGGPILHPALSGLVLVPIAPHALSNRPIVIPHDAEVVIQVT
SGRDASVNFDMQSLTSLLPGDRIVVRRSERTVRLLHPVGYNYYATLRKKLHWHEYPTEDNRL

Sequences:

>Translated_302_residues
MTSRANATSPFKTVALVGRYSAANIAAPLLELASCIAARGHDIVFERETALNIGVQDYPALPPDEMARHADVAVVLGGDG
TLLGIGRHLAGASVPVIGVNHGRLGFMTDIPFEDVHNVLPDMLAGQYEAETRSLLQAQVVRDDETIFSALAFNDVVVNRS
GFSGMVELAVSVDGFFMYNQRSDGLIVSTPTGSTAYALSAGGPILHPALSGLVLVPIAPHALSNRPIVIPHDAEVVIQVT
SGRDASVNFDMQSLTSLLPGDRIVVRRSERTVRLLHPVGYNYYATLRKKLHWHEYPTEDNRL
>Mature_301_residues
TSRANATSPFKTVALVGRYSAANIAAPLLELASCIAARGHDIVFERETALNIGVQDYPALPPDEMARHADVAVVLGGDGT
LLGIGRHLAGASVPVIGVNHGRLGFMTDIPFEDVHNVLPDMLAGQYEAETRSLLQAQVVRDDETIFSALAFNDVVVNRSG
FSGMVELAVSVDGFFMYNQRSDGLIVSTPTGSTAYALSAGGPILHPALSGLVLVPIAPHALSNRPIVIPHDAEVVIQVTS
GRDASVNFDMQSLTSLLPGDRIVVRRSERTVRLLHPVGYNYYATLRKKLHWHEYPTEDNRL

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Homo sapiens, GI55743112, Length=330, Percent_Identity=26.969696969697, Blast_Score=105, Evalue=7e-23,
Organism=Escherichia coli, GI1788968, Length=285, Percent_Identity=39.2982456140351, Blast_Score=211, Evalue=4e-56,
Organism=Saccharomyces cerevisiae, GI6320794, Length=241, Percent_Identity=29.8755186721992, Blast_Score=123, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6322509, Length=242, Percent_Identity=31.8181818181818, Blast_Score=116, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6325068, Length=182, Percent_Identity=31.8681318681319, Blast_Score=112, Evalue=5e-26,
Organism=Drosophila melanogaster, GI28573832, Length=334, Percent_Identity=29.3413173652695, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI28573826, Length=334, Percent_Identity=29.3413173652695, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI161077047, Length=334, Percent_Identity=29.3413173652695, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI28573830, Length=334, Percent_Identity=29.3413173652695, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI28573828, Length=328, Percent_Identity=29.5731707317073, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI20129957, Length=283, Percent_Identity=28.2685512367491, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI281363323, Length=283, Percent_Identity=28.2685512367491, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI281363321, Length=283, Percent_Identity=28.2685512367491, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24653422, Length=283, Percent_Identity=28.2685512367491, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24653424, Length=283, Percent_Identity=28.2685512367491, Blast_Score=88, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK_RALSO (Q8XW25)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_520771.1
- ProteinModelPortal:   Q8XW25
- SMR:   Q8XW25
- GeneID:   1221497
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc2650
- NMPDR:   fig|267608.1.peg.2650
- HOGENOM:   HBG713904
- OMA:   FESFMID
- ProtClustDB:   PRK02155
- BioCyc:   RSOL267608:RSC2650-MONOMER
- BRENDA:   2.7.1.23
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 32528; Mature: 32397

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSRANATSPFKTVALVGRYSAANIAAPLLELASCIAARGHDIVFERETALNIGVQDYPA
CCCCCCCCCCHHEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEECCEEECCCCCCCC
LPPDEMARHADVAVVLGGDGTLLGIGRHLAGASVPVIGVNHGRLGFMTDIPFEDVHNVLP
CCHHHHHCCCCEEEEECCCCEEEEECHHHCCCCCCEEECCCCCEEEEECCCHHHHHHHHH
DMLAGQYEAETRSLLQAQVVRDDETIFSALAFNDVVVNRSGFSGMVELAVSVDGFFMYNQ
HHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCEEEEECCCCCCEEEEEEEECEEEEEEC
RSDGLIVSTPTGSTAYALSAGGPILHPALSGLVLVPIAPHALSNRPIVIPHDAEVVIQVT
CCCCEEEECCCCCEEEEEECCCCCHHHHHCCEEEEEECCHHCCCCCEEEECCCEEEEEEE
SGRDASVNFDMQSLTSLLPGDRIVVRRSERTVRLLHPVGYNYYATLRKKLHWHEYPTEDN
CCCCCEEEECHHHHHHHCCCCEEEEEECCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCC
RL
CC
>Mature Secondary Structure 
TSRANATSPFKTVALVGRYSAANIAAPLLELASCIAARGHDIVFERETALNIGVQDYPA
CCCCCCCCCHHEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEECCEEECCCCCCCC
LPPDEMARHADVAVVLGGDGTLLGIGRHLAGASVPVIGVNHGRLGFMTDIPFEDVHNVLP
CCHHHHHCCCCEEEEECCCCEEEEECHHHCCCCCCEEECCCCCEEEEECCCHHHHHHHHH
DMLAGQYEAETRSLLQAQVVRDDETIFSALAFNDVVVNRSGFSGMVELAVSVDGFFMYNQ
HHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCEEEEECCCCCCEEEEEEEECEEEEEEC
RSDGLIVSTPTGSTAYALSAGGPILHPALSGLVLVPIAPHALSNRPIVIPHDAEVVIQVT
CCCCEEEECCCCCEEEEEECCCCCHHHHHCCEEEEEECCHHCCCCCEEEECCCEEEEEEE
SGRDASVNFDMQSLTSLLPGDRIVVRRSERTVRLLHPVGYNYYATLRKKLHWHEYPTEDN
CCCCCEEEECHHHHHHHCCCCEEEEEECCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCC
RL
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852