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The map label for this gene is pcnB [H]

Identifier: 17547346

GI number: 17547346

Start: 2829832

End: 2831376

Strand: Direct

Name: pcnB [H]

Synonym: RSc2627

Alternate gene names: 17547346

Gene position: 2829832-2831376 (Clockwise)

Preceding gene: 17547345

Following gene: 17547347

Centisome position: 76.14

GC content: 70.94

Gene sequence:

>1545_bases
GTGATCAAGAAACTCATCAATCGCCTGCTGGGCAGGCCCGCCGCGGAGACCGCGCCCGCCACCGAGCCCGGCACGCCGGC
CAGGAAGGCCCGCGCCCCCAAGACCCCGAAGGCGCGCGCCAAGGCCGCCGCCGGGGGCGTGCCGCGCGCGCCGCGCGTGT
GGAGCGTGGAAGAGCACGGCATCGACCCGAAGCTGCTGTCGCGCAACGCCGTCAAGGTCACGTCCACGCTGCAGGAAGCC
GGCTATGACGCCTTCATCGTGGGCGGCGCGGTGCGCGATCTGCTGCTGGGCATCAAGCCAAAGGACTTCGACGTCGCCAC
CAACGCCACGCCCGAGCAGGTGGAGGCGCTATTCCGCCGCTCGCGCATCATCGGCCGGCGCTTCCAGATCGTGCACGTGA
CGTTCTACGGCGGGCGCGAGCAGGAGATCATCGAGGTGTCGACCTTCCGCGCGCTGGTCGACGCCGTGGAAAGCGAGCAG
GTACCGGCCGGCAAGCGCGTCAAGCGCACCGAGCTGGACCACCACACCCACGCCGTCGACGCCAGCGGCCGCGTGCTGCG
CGACAACGTGTGGGGTACCCAGGCCGAGGACGCCACCCGCCGCGACTTCACCGTCAACGCGATGTACTACAACCCGGCCG
ACCAGACCGTGCACGACTACCACCGCGGCATGGAAGACATGCGCGCGCGCCTGCTGCGCATGATCGGCGATCCGCATACG
CGCTACCGCGAAGACCCGGTGCGCATGCTGCGCGTGGTGCGCTTCGCGGCCAAGACCGGCTTCGACATCGATCCGGCCAC
GCGCGCGCCGATCGCCGAGCTGGGGCCGCTGATCCACAACGTGCCGGCCGCGCGGCTGTTCGACGAAATGCTCAAGCTGC
TGATGTCGGGCCACGCCTGGGCATCGCTGCAGGAGCTGCGCAAGGCCGGCCTGCACCGCGGCCTGCTGCCGCTGCTGGAC
GTGGCGCTCGAGCAGCCGATGGGCCAGCGCTTCGTGCAGCTGGCGCTCGACAACACCGATGAGCGCGTCAAGGCCGGCAA
GCCGGTCTCGCCCGGCTTCCTGTTCGCCTCGCTGATGTGGCACCACGTGCTGGAGCACTGGAACCGCCGCCGCGCCGCCG
GCGAGCCGCTGATCCCCGCGCTGCACGAGGCGATGGACGAAGTCCTCGACCGCCAGACCGAGCAGCTCGCCATCCAGCGC
CGCTTCACCGCCGACATGAAGGAGATCTGGAGCATGCAGCCGCGCTTCGAGAAGCGTGTTGGCCGCATGCCCCACCGTCT
GCTCGAATCGCCGCGCTTCCGCGCCGGCTACGACTTCCTGGTCCTGCGCTGCGCCTCGGGCGAATTGCCGCAGGAGCTCG
CCGCCTGGTGGACCGACTTCCAGAACGGCGACGGCGAGGCGCGCGAAGCGCTGATCGCGCAGGCCAAGCAGGCGCCGGCC
GCCGCCGGGGGCGCCCCCGCGGGCGCCAAGCGCCGCCGCCGGCGTGGTCCGGCAAAATCGGATACAGTCGCCGACGTGAA
CCCGGGCAGTTCGGAGGGAACGTGA

Upstream 100 bases:

>100_bases
AAGTGACCGACCCGGTCGCCACCAACCCCGACGACACCTTGCGGCACCTCGCCGCCCAGCGCAACTGGCGCATCATGGAC
CTCTTCTGAAAGAGCGCGGC

Downstream 100 bases:

>100_bases
TGACAGTGGCGTATATCGGCATCGGCGCCAACCTCGGCGACGCGCGCCAGGCCGTCAAAGACGCCGTGGTCTGCCTCGCG
CAGCAGGTGGGCATCAGCAT

Product: polynucleotide adenylyltransferase

Products: NA

Alternate protein names: PAP [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MIKKLINRLLGRPAAETAPATEPGTPARKARAPKTPKARAKAAAGGVPRAPRVWSVEEHGIDPKLLSRNAVKVTSTLQEA
GYDAFIVGGAVRDLLLGIKPKDFDVATNATPEQVEALFRRSRIIGRRFQIVHVTFYGGREQEIIEVSTFRALVDAVESEQ
VPAGKRVKRTELDHHTHAVDASGRVLRDNVWGTQAEDATRRDFTVNAMYYNPADQTVHDYHRGMEDMRARLLRMIGDPHT
RYREDPVRMLRVVRFAAKTGFDIDPATRAPIAELGPLIHNVPAARLFDEMLKLLMSGHAWASLQELRKAGLHRGLLPLLD
VALEQPMGQRFVQLALDNTDERVKAGKPVSPGFLFASLMWHHVLEHWNRRRAAGEPLIPALHEAMDEVLDRQTEQLAIQR
RFTADMKEIWSMQPRFEKRVGRMPHRLLESPRFRAGYDFLVLRCASGELPQELAAWWTDFQNGDGEAREALIAQAKQAPA
AAGGAPAGAKRRRRRGPAKSDTVADVNPGSSEGT

Sequences:

>Translated_514_residues
MIKKLINRLLGRPAAETAPATEPGTPARKARAPKTPKARAKAAAGGVPRAPRVWSVEEHGIDPKLLSRNAVKVTSTLQEA
GYDAFIVGGAVRDLLLGIKPKDFDVATNATPEQVEALFRRSRIIGRRFQIVHVTFYGGREQEIIEVSTFRALVDAVESEQ
VPAGKRVKRTELDHHTHAVDASGRVLRDNVWGTQAEDATRRDFTVNAMYYNPADQTVHDYHRGMEDMRARLLRMIGDPHT
RYREDPVRMLRVVRFAAKTGFDIDPATRAPIAELGPLIHNVPAARLFDEMLKLLMSGHAWASLQELRKAGLHRGLLPLLD
VALEQPMGQRFVQLALDNTDERVKAGKPVSPGFLFASLMWHHVLEHWNRRRAAGEPLIPALHEAMDEVLDRQTEQLAIQR
RFTADMKEIWSMQPRFEKRVGRMPHRLLESPRFRAGYDFLVLRCASGELPQELAAWWTDFQNGDGEAREALIAQAKQAPA
AAGGAPAGAKRRRRRGPAKSDTVADVNPGSSEGT
>Mature_514_residues
MIKKLINRLLGRPAAETAPATEPGTPARKARAPKTPKARAKAAAGGVPRAPRVWSVEEHGIDPKLLSRNAVKVTSTLQEA
GYDAFIVGGAVRDLLLGIKPKDFDVATNATPEQVEALFRRSRIIGRRFQIVHVTFYGGREQEIIEVSTFRALVDAVESEQ
VPAGKRVKRTELDHHTHAVDASGRVLRDNVWGTQAEDATRRDFTVNAMYYNPADQTVHDYHRGMEDMRARLLRMIGDPHT
RYREDPVRMLRVVRFAAKTGFDIDPATRAPIAELGPLIHNVPAARLFDEMLKLLMSGHAWASLQELRKAGLHRGLLPLLD
VALEQPMGQRFVQLALDNTDERVKAGKPVSPGFLFASLMWHHVLEHWNRRRAAGEPLIPALHEAMDEVLDRQTEQLAIQR
RFTADMKEIWSMQPRFEKRVGRMPHRLLESPRFRAGYDFLVLRCASGELPQELAAWWTDFQNGDGEAREALIAQAKQAPA
AAGGAPAGAKRRRRRGPAKSDTVADVNPGSSEGT

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=241, Percent_Identity=31.1203319502075, Blast_Score=100, Evalue=4e-21,
Organism=Escherichia coli, GI87081691, Length=443, Percent_Identity=43.7923250564334, Blast_Score=338, Evalue=7e-94,
Organism=Saccharomyces cerevisiae, GI6321016, Length=244, Percent_Identity=30.327868852459, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI281360112, Length=299, Percent_Identity=28.7625418060201, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI21357337, Length=299, Percent_Identity=28.7625418060201, Blast_Score=96, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 57330; Mature: 57330

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKLINRLLGRPAAETAPATEPGTPARKARAPKTPKARAKAAAGGVPRAPRVWSVEEHG
CHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHCCCCCCCCCEECHHHHC
IDPKLLSRNAVKVTSTLQEAGYDAFIVGGAVRDLLLGIKPKDFDVATNATPEQVEALFRR
CCHHHHCCCHHHHHHHHHHCCCCEEEECHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
SRIIGRRFQIVHVTFYGGREQEIIEVSTFRALVDAVESEQVPAGKRVKRTELDHHTHAVD
HHHHCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEEC
ASGRVLRDNVWGTQAEDATRRDFTVNAMYYNPADQTVHDYHRGMEDMRARLLRMIGDPHT
CCCCEEECCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHCCHHHHHHHHHHHHCCCHH
RYREDPVRMLRVVRFAAKTGFDIDPATRAPIAELGPLIHNVPAARLFDEMLKLLMSGHAW
HHHCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCHH
ASLQELRKAGLHRGLLPLLDVALEQPMGQRFVQLALDNTDERVKAGKPVSPGFLFASLMW
HHHHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHH
HHVLEHWNRRRAAGEPLIPALHEAMDEVLDRQTEQLAIQRRFTADMKEIWSMQPRFEKRV
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
GRMPHRLLESPRFRAGYDFLVLRCASGELPQELAAWWTDFQNGDGEAREALIAQAKQAPA
HHCHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
AAGGAPAGAKRRRRRGPAKSDTVADVNPGSSEGT
CCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIKKLINRLLGRPAAETAPATEPGTPARKARAPKTPKARAKAAAGGVPRAPRVWSVEEHG
CHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHCCCCCCCCCEECHHHHC
IDPKLLSRNAVKVTSTLQEAGYDAFIVGGAVRDLLLGIKPKDFDVATNATPEQVEALFRR
CCHHHHCCCHHHHHHHHHHCCCCEEEECHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
SRIIGRRFQIVHVTFYGGREQEIIEVSTFRALVDAVESEQVPAGKRVKRTELDHHTHAVD
HHHHCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEEC
ASGRVLRDNVWGTQAEDATRRDFTVNAMYYNPADQTVHDYHRGMEDMRARLLRMIGDPHT
CCCCEEECCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHCCHHHHHHHHHHHHCCCHH
RYREDPVRMLRVVRFAAKTGFDIDPATRAPIAELGPLIHNVPAARLFDEMLKLLMSGHAW
HHHCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCHH
ASLQELRKAGLHRGLLPLLDVALEQPMGQRFVQLALDNTDERVKAGKPVSPGFLFASLMW
HHHHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHH
HHVLEHWNRRRAAGEPLIPALHEAMDEVLDRQTEQLAIQRRFTADMKEIWSMQPRFEKRV
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
GRMPHRLLESPRFRAGYDFLVLRCASGELPQELAAWWTDFQNGDGEAREALIAQAKQAPA
HHCHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
AAGGAPAGAKRRRRRGPAKSDTVADVNPGSSEGT
CCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]