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The map label for this gene is ada [H]

Identifier: 17547289

GI number: 17547289

Start: 2778230

End: 2779312

Strand: Direct

Name: ada [H]

Synonym: RSc2570

Alternate gene names: 17547289

Gene position: 2778230-2779312 (Clockwise)

Preceding gene: 17547288

Following gene: 17547290

Centisome position: 74.76

GC content: 73.22

Gene sequence:

>1083_bases
TTGACGTCATCCGACACCATGCCAACGCCCGCACCGTCCACCATCGAACACGACCCGCGCTGGGCCCGCGTGCTCGCGCG
CGATGCGTCCGCCGACGGCCAGTTCGTCTACGCCGTGAAGACCACGGGCGTGTACTGCCAGCCGAGCAGCCCGTCGCGGC
TGCCGCGCCCCGAGAACGTCGAATTCTTCGACACGCCCGCCGAGGCCGAGGCCGCCGGCTATCGCCCCAGCCGCCGCGCC
ACGCCGGACCAGACCACGGCCCGCGCGCAGCACGCCGCGCGGGTCGCCGCCGCGTGCCGCCGCATCGAAGCAGCCGACAC
GCCGCCCGCGCTCGAAGCGCTCGCGCGGGAGGCCGGCCTGAGCCCGTACCACTTCCACCGGCTGTTCAAGACCGTCACCG
GCCTGACGCCCAAGGCCTACGCCGCGGCGCACCGTGCGCGCAAGCTGCGCGCGCAGCTCGGCCGCGGCAGCTCCGTCACC
GAGGCCATCTACGACGCCGGCTTCAATGCCAGCAGCCGCTTCTACGAGGCGTCCGACGGCGTGCTCGGCATGACGGCCAG
CCGCTACCGCGCGGGCGGCGCGCAGACGACGATCCGCTTTGCCGTGGGCGAATGCTCGCTGGGCGCGATCCTGGTGGCGC
AGAGCGAACGCGGCATCTGCGCGATCCTGCTGGGCGAGGATCCGCAGGCACTGGTGCACGACCTGCAGGACCAGTTCCCC
AAGGCCGAACTGATCGGCGGTGACGCGGATTTCGAAGACCTGGTCGCCAGGGTGGTCGGGCTGATCGAAGCGCCCTCGAT
CGGGCTGGACCTGCCGCTGGACGTGCGCGGCACCGCGTTCCAGGAGCGCGTGTGGCAGGCATTGCGCGAGATTCCGCCCG
GCACCACGGCGAGCTACGCGGAGATCGCCGCGCGCATCGGCGCGCCCAGGGCGGTGCGCGCGGTGGCGCAGGCGTGCGGC
GCCAACCATCTGGCGGTGGCCATCCCCTGCCACCGCGTGGTGCGCAGCGACGGCCAGCTCTCCGGCTACCGCTGGGGCGT
GGAGCGCAAGCGCGCGCTGCTGGAGCGCGAAGCGCAAGGCTGA

Upstream 100 bases:

>100_bases
CGCATGCCGGCCGGCTGAAGCGCCGCCGCCGCCGCGCCGAATTCGACCGCAAGCGGCGGAGTGCCCCTGACCGGGCACGC
GCCTACAGTACAGGCATCGA

Downstream 100 bases:

>100_bases
TGACGGCGAAGACCTGGACGCTGCTCGGCGCCAGCGGCAAACCGTACGACAGCCCCATCCCCGGCACGCTGGGCGGCCAC
CGGCGCGCGCGCATCTACGA

Product: bifunctional methylated-DNA--protein-cysteine methyltransferase/O-6-methylguanine-DNA transcription regulator

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MTSSDTMPTPAPSTIEHDPRWARVLARDASADGQFVYAVKTTGVYCQPSSPSRLPRPENVEFFDTPAEAEAAGYRPSRRA
TPDQTTARAQHAARVAAACRRIEAADTPPALEALAREAGLSPYHFHRLFKTVTGLTPKAYAAAHRARKLRAQLGRGSSVT
EAIYDAGFNASSRFYEASDGVLGMTASRYRAGGAQTTIRFAVGECSLGAILVAQSERGICAILLGEDPQALVHDLQDQFP
KAELIGGDADFEDLVARVVGLIEAPSIGLDLPLDVRGTAFQERVWQALREIPPGTTASYAEIAARIGAPRAVRAVAQACG
ANHLAVAIPCHRVVRSDGQLSGYRWGVERKRALLEREAQG

Sequences:

>Translated_360_residues
MTSSDTMPTPAPSTIEHDPRWARVLARDASADGQFVYAVKTTGVYCQPSSPSRLPRPENVEFFDTPAEAEAAGYRPSRRA
TPDQTTARAQHAARVAAACRRIEAADTPPALEALAREAGLSPYHFHRLFKTVTGLTPKAYAAAHRARKLRAQLGRGSSVT
EAIYDAGFNASSRFYEASDGVLGMTASRYRAGGAQTTIRFAVGECSLGAILVAQSERGICAILLGEDPQALVHDLQDQFP
KAELIGGDADFEDLVARVVGLIEAPSIGLDLPLDVRGTAFQERVWQALREIPPGTTASYAEIAARIGAPRAVRAVAQACG
ANHLAVAIPCHRVVRSDGQLSGYRWGVERKRALLEREAQG
>Mature_359_residues
TSSDTMPTPAPSTIEHDPRWARVLARDASADGQFVYAVKTTGVYCQPSSPSRLPRPENVEFFDTPAEAEAAGYRPSRRAT
PDQTTARAQHAARVAAACRRIEAADTPPALEALAREAGLSPYHFHRLFKTVTGLTPKAYAAAHRARKLRAQLGRGSSVTE
AIYDAGFNASSRFYEASDGVLGMTASRYRAGGAQTTIRFAVGECSLGAILVAQSERGICAILLGEDPQALVHDLQDQFPK
AELIGGDADFEDLVARVVGLIEAPSIGLDLPLDVRGTAFQERVWQALREIPPGTTASYAEIAARIGAPRAVRAVAQACGA
NHLAVAIPCHRVVRSDGQLSGYRWGVERKRALLEREAQG

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG2169

COG function: function code F; Adenosine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=348, Percent_Identity=52.2988505747126, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI1787596, Length=93, Percent_Identity=44.0860215053763, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI115533070, Length=131, Percent_Identity=42.7480916030534, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI115533068, Length=131, Percent_Identity=42.7480916030534, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320001, Length=84, Percent_Identity=45.2380952380952, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI17137554, Length=173, Percent_Identity=35.2601156069364, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 38615; Mature: 38484

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSDTMPTPAPSTIEHDPRWARVLARDASADGQFVYAVKTTGVYCQPSSPSRLPRPENV
CCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCEEECCCCCCCCCCCCCC
EFFDTPAEAEAAGYRPSRRATPDQTTARAQHAARVAAACRRIEAADTPPALEALAREAGL
CEECCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
SPYHFHRLFKTVTGLTPKAYAAAHRARKLRAQLGRGSSVTEAIYDAGFNASSRFYEASDG
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEEECCCC
VLGMTASRYRAGGAQTTIRFAVGECSLGAILVAQSERGICAILLGEDPQALVHDLQDQFP
EEECCHHHHHCCCCCEEEEEEECCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHCC
KAELIGGDADFEDLVARVVGLIEAPSIGLDLPLDVRGTAFQERVWQALREIPPGTTASYA
CEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHH
EIAARIGAPRAVRAVAQACGANHLAVAIPCHRVVRSDGQLSGYRWGVERKRALLEREAQG
HHHHHHCCCHHHHHHHHHCCCCCEEEEECHHHHHHCCCCCCCEECCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TSSDTMPTPAPSTIEHDPRWARVLARDASADGQFVYAVKTTGVYCQPSSPSRLPRPENV
CCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCEEECCCCCCCCCCCCCC
EFFDTPAEAEAAGYRPSRRATPDQTTARAQHAARVAAACRRIEAADTPPALEALAREAGL
CEECCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
SPYHFHRLFKTVTGLTPKAYAAAHRARKLRAQLGRGSSVTEAIYDAGFNASSRFYEASDG
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEEECCCC
VLGMTASRYRAGGAQTTIRFAVGECSLGAILVAQSERGICAILLGEDPQALVHDLQDQFP
EEECCHHHHHCCCCCEEEEEEECCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHCC
KAELIGGDADFEDLVARVVGLIEAPSIGLDLPLDVRGTAFQERVWQALREIPPGTTASYA
CEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHH
EIAARIGAPRAVRAVAQACGANHLAVAIPCHRVVRSDGQLSGYRWGVERKRALLEREAQG
HHHHHHCCCHHHHHHHHHCCCCCEEEEECHHHHHHCCCCCCCEECCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]