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The map label for this gene is alkA [H]

Identifier: 17547288

GI number: 17547288

Start: 2777509

End: 2778147

Strand: Direct

Name: alkA [H]

Synonym: RSc2569

Alternate gene names: 17547288

Gene position: 2777509-2778147 (Clockwise)

Preceding gene: 17547287

Following gene: 17547289

Centisome position: 74.74

GC content: 71.67

Gene sequence:

>639_bases
ATGAGCATCGCCGCCGACCTGTCCGAACGCACCTACCGGCAAGCCACCGCGTTCCTGTCGTCGTTGGACGACGACTGGGC
GCGCCACATCGCCGCCATCGGCCCCTGCCGGCACACCGCCAAGCCCGCGCGCGACCCGTACGAGGCGCTGGTGCGCGCCA
TCGCCTATCAGCAGCTGCATGCCAGGGCCGGCGACGCCATCCTCGGCCGCCTGCTGGCGCGGTATCCCGGTGTCGATTTC
CCATCGCCCCGGCAATTGCTGGAGACCGACGAGGCCACGCTGCGCGGCTGCGGCTTCTCGGCGACCAAGCTGGCGACGAT
CCGCGGCATCGCGCAGGCCACGCTCGACAGCATCGTGCCCACGCGCGACGAAGCGCTCGCCCTGCCGGACGAGGCGCTGA
TCGAGCGGCTCGTCACCCTGCGCGGCGTCGGGCGGTGGACGGTGGAAATGCTGCTGATCTACACGCTGGAGCGCGCCGAC
ATCCTGCCCGCCGACGACTTCGGCGTGCGCGAGGGCTACCGTCGCCTCAAGCGCCTGGAAACCGCCCCCACGCGCAGACA
GATGGCGGAGATCGGCCGCGCCTGGAGCCCCTACCGCACCGTCGCCGCGTGGTATCTGTGGCGCATGCCGGCCGGCTGA

Upstream 100 bases:

>100_bases
GATCGCCTGCGCTACCACGGCGTCCTGCCGCTCAAGGAAGCCGCGCATCCGCTGCTGGGCGCGCAGCGCATCAACCTGAC
CTTGCGCCGGGCCGGCTGAC

Downstream 100 bases:

>100_bases
AGCGCCGCCGCCGCCGCGCCGAATTCGACCGCAAGCGGCGGAGTGCCCCTGACCGGGCACGCGCCTACAGTACAGGCATC
GATTGACGTCATCCGACACC

Product: DNA-3-methyladenine glycosylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MSIAADLSERTYRQATAFLSSLDDDWARHIAAIGPCRHTAKPARDPYEALVRAIAYQQLHARAGDAILGRLLARYPGVDF
PSPRQLLETDEATLRGCGFSATKLATIRGIAQATLDSIVPTRDEALALPDEALIERLVTLRGVGRWTVEMLLIYTLERAD
ILPADDFGVREGYRRLKRLETAPTRRQMAEIGRAWSPYRTVAAWYLWRMPAG

Sequences:

>Translated_212_residues
MSIAADLSERTYRQATAFLSSLDDDWARHIAAIGPCRHTAKPARDPYEALVRAIAYQQLHARAGDAILGRLLARYPGVDF
PSPRQLLETDEATLRGCGFSATKLATIRGIAQATLDSIVPTRDEALALPDEALIERLVTLRGVGRWTVEMLLIYTLERAD
ILPADDFGVREGYRRLKRLETAPTRRQMAEIGRAWSPYRTVAAWYLWRMPAG
>Mature_211_residues
SIAADLSERTYRQATAFLSSLDDDWARHIAAIGPCRHTAKPARDPYEALVRAIAYQQLHARAGDAILGRLLARYPGVDFP
SPRQLLETDEATLRGCGFSATKLATIRGIAQATLDSIVPTRDEALALPDEALIERLVTLRGVGRWTVEMLLIYTLERADI
LPADDFGVREGYRRLKRLETAPTRRQMAEIGRAWSPYRTVAAWYLWRMPAG

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, 3-methylguanine, 7-methylguanine, O2- methylthymine, and O2-methylcytosine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkylbase DNA glycosidase alkA family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00730 HhH-GPD [H]

EC number: =3.2.2.21 [H]

Molecular weight: Translated: 23735; Mature: 23604

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIAADLSERTYRQATAFLSSLDDDWARHIAAIGPCRHTAKPARDPYEALVRAIAYQQLH
CCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHH
ARAGDAILGRLLARYPGVDFPSPRQLLETDEATLRGCGFSATKLATIRGIAQATLDSIVP
HHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
TRDEALALPDEALIERLVTLRGVGRWTVEMLLIYTLERADILPADDFGVREGYRRLKRLE
CCCCHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
TAPTRRQMAEIGRAWSPYRTVAAWYLWRMPAG
CCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SIAADLSERTYRQATAFLSSLDDDWARHIAAIGPCRHTAKPARDPYEALVRAIAYQQLH
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHH
ARAGDAILGRLLARYPGVDFPSPRQLLETDEATLRGCGFSATKLATIRGIAQATLDSIVP
HHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
TRDEALALPDEALIERLVTLRGVGRWTVEMLLIYTLERADILPADDFGVREGYRRLKRLE
CCCCHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
TAPTRRQMAEIGRAWSPYRTVAAWYLWRMPAG
CCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]