Click here to switch to the map view.

The map label for this gene is bkdB [H]

Identifier: 17546518

GI number: 17546518

Start: 1934899

End: 1936017

Strand: Direct

Name: bkdB [H]

Synonym: RSc1799

Alternate gene names: 17546518

Gene position: 1934899-1936017 (Clockwise)

Preceding gene: 17546517

Following gene: 17546519

Centisome position: 52.06

GC content: 73.28

Gene sequence:

>1119_bases
ATGATCGTCTTCAAACTGCCGGATCTCGGCGAAGGGCTGCAGGAGGCGGAGATCGTGCAATGGCACGTGCAGGCCGGCGA
CACCGTCGAGGCCGATCAGCCGCTGGTCTCGGTCGAGACCGCCAAGGCCATCGTCGAGATTCCGTCGCCGCAGGCCGGCC
GCATCGCCAGGCTGTTCGGCCAGCCGGGCGACATCGTGCACCTGGGCGCGCCGCTGGTCGCGTTCGAAGGGGCGGGCGGC
GGTGAAGCGGATGCGGGCACCGTGGTCGGGCAGATGCAGGTCGGGCAACAGGTGGTGCGGGAGGCGCCGGCCGTGCTGGC
CGCCGCGCCCGGGGCGGCCGCCGGCGGCATCAAGGCGACGCCGGCGGTGCGGGCGCTGGCGCGCAAGCTCGACGTGGACC
TGTCGATGGCGACGCCCTCGGGGCCGGACGGGGTCATCACGGCGGCGGATGTCGAGCGCGTGGCCAAGGTGTTCTCCGAA
CTGGGGCCGCCCGAGGTGCTGCGCGGCGTGCGGCGCGCCATGGCGCAGAACATGGCGCGCGCGCAGAGCGAAGTGGCCGC
CGCCACCGTCATTGACGATGCCGACATCCACGCTTGGCCGCCCGGCACCGATGTGACCATCCGCCTGATCCGCGCGCTGG
TGGCCGGCTGCCGCGCCGAGCCCGGGCTCAACGCCTGGTTCGACGGGCATGCGGGGCGGCGCCACGTGCTGGAGAAGATC
GACCTCGGCATCGCGGTCGATCTGCCGGACGGGCTGTTCGTGCCGGTGCTGCGCAATGTCGCGCACCGCGACGCGGCCGA
TCTGCGCGCCGGGCTGGACCGCATGCGCGCCGACATCCGGGCACGCAAGATTCCGCCGGAGGAGTTGCGCGGCAACACCA
TCACGCTGTCGAACTTCGGCATGATCGCGGGCAAGTACGCGGCGCCGGTGGTGGTGCCGCCGACCGTGGCGATCCTGGGC
GCGGGCCGCATCCACGAGCAGGTGGTGGCGGCCGCCGGTGCCCCGGCGGTGCACCGCGTGCTGCCGCTGAGCCTGACCTT
CGACCACCGGGTGGTGACCGGCGGCGAGGCGGCGCGGTTCCTGGCCGCGACGATCGCGGACCTGGAAGCGGCGCACTAA

Upstream 100 bases:

>100_bases
ACGACGTGGTCATGCCGCTGCCCAGGCTGGAGAACCAATACCTGCCCGGCGTCGAGCGCATCCTCGCCGCGGTCAGGAAG
ACCCTGGAGGCCGCCTGACG

Downstream 100 bases:

>100_bases
GCCCCAGGCCGGCCGAGCCGGCCCGCAGCCCTGGCCCGGCAACGTGAAGCGGACGTGACCGGCACGCGGCGGCGGCGGGT
GGCATCGCTTCGCCGTTCGC

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MIVFKLPDLGEGLQEAEIVQWHVQAGDTVEADQPLVSVETAKAIVEIPSPQAGRIARLFGQPGDIVHLGAPLVAFEGAGG
GEADAGTVVGQMQVGQQVVREAPAVLAAAPGAAAGGIKATPAVRALARKLDVDLSMATPSGPDGVITAADVERVAKVFSE
LGPPEVLRGVRRAMAQNMARAQSEVAAATVIDDADIHAWPPGTDVTIRLIRALVAGCRAEPGLNAWFDGHAGRRHVLEKI
DLGIAVDLPDGLFVPVLRNVAHRDAADLRAGLDRMRADIRARKIPPEELRGNTITLSNFGMIAGKYAAPVVVPPTVAILG
AGRIHEQVVAAAGAPAVHRVLPLSLTFDHRVVTGGEAARFLAATIADLEAAH

Sequences:

>Translated_372_residues
MIVFKLPDLGEGLQEAEIVQWHVQAGDTVEADQPLVSVETAKAIVEIPSPQAGRIARLFGQPGDIVHLGAPLVAFEGAGG
GEADAGTVVGQMQVGQQVVREAPAVLAAAPGAAAGGIKATPAVRALARKLDVDLSMATPSGPDGVITAADVERVAKVFSE
LGPPEVLRGVRRAMAQNMARAQSEVAAATVIDDADIHAWPPGTDVTIRLIRALVAGCRAEPGLNAWFDGHAGRRHVLEKI
DLGIAVDLPDGLFVPVLRNVAHRDAADLRAGLDRMRADIRARKIPPEELRGNTITLSNFGMIAGKYAAPVVVPPTVAILG
AGRIHEQVVAAAGAPAVHRVLPLSLTFDHRVVTGGEAARFLAATIADLEAAH
>Mature_372_residues
MIVFKLPDLGEGLQEAEIVQWHVQAGDTVEADQPLVSVETAKAIVEIPSPQAGRIARLFGQPGDIVHLGAPLVAFEGAGG
GEADAGTVVGQMQVGQQVVREAPAVLAAAPGAAAGGIKATPAVRALARKLDVDLSMATPSGPDGVITAADVERVAKVFSE
LGPPEVLRGVRRAMAQNMARAQSEVAAATVIDDADIHAWPPGTDVTIRLIRALVAGCRAEPGLNAWFDGHAGRRHVLEKI
DLGIAVDLPDGLFVPVLRNVAHRDAADLRAGLDRMRADIRARKIPPEELRGNTITLSNFGMIAGKYAAPVVVPPTVAILG
AGRIHEQVVAAAGAPAVHRVLPLSLTFDHRVVTGGEAARFLAATIADLEAAH

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=415, Percent_Identity=27.9518072289157, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI19923748, Length=220, Percent_Identity=28.6363636363636, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI31711992, Length=430, Percent_Identity=23.0232558139535, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI203098753, Length=445, Percent_Identity=22.4719101123595, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI203098816, Length=446, Percent_Identity=22.1973094170404, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1786305, Length=418, Percent_Identity=28.2296650717703, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1786946, Length=403, Percent_Identity=26.302729528536, Blast_Score=111, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI25146366, Length=399, Percent_Identity=28.0701754385965, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17537937, Length=420, Percent_Identity=25.7142857142857, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17560088, Length=432, Percent_Identity=24.0740740740741, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17538894, Length=165, Percent_Identity=27.8787878787879, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320352, Length=390, Percent_Identity=25.3846153846154, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6324258, Length=157, Percent_Identity=26.1146496815287, Blast_Score=73, Evalue=7e-14,
Organism=Drosophila melanogaster, GI18859875, Length=414, Percent_Identity=25.1207729468599, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24645909, Length=152, Percent_Identity=35.5263157894737, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24582497, Length=158, Percent_Identity=31.6455696202532, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI20129315, Length=158, Percent_Identity=31.6455696202532, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.168 [H]

Molecular weight: Translated: 38657; Mature: 38657

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVFKLPDLGEGLQEAEIVQWHVQAGDTVEADQPLVSVETAKAIVEIPSPQAGRIARLFG
CEEEECCCCCCCCHHHHEEEEEECCCCCCCCCCCEEEHHHHHHEEECCCCCCCHHHHHHC
QPGDIVHLGAPLVAFEGAGGGEADAGTVVGQMQVGQQVVREAPAVLAAAPGAAAGGIKAT
CCCCEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCC
PAVRALARKLDVDLSMATPSGPDGVITAADVERVAKVFSELGPPEVLRGVRRAMAQNMAR
HHHHHHHHHHCCCEEECCCCCCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
AQSEVAAATVIDDADIHAWPPGTDVTIRLIRALVAGCRAEPGLNAWFDGHAGRRHVLEKI
HHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
DLGIAVDLPDGLFVPVLRNVAHRDAADLRAGLDRMRADIRARKIPPEELRGNTITLSNFG
CCCEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCEEEEECCC
MIAGKYAAPVVVPPTVAILGAGRIHEQVVAAAGAPAVHRVLPLSLTFDHRVVTGGEAARF
EEECCCCCCEECCCHHEEECCCHHHHHHHHHCCCCHHHHHEEEEEEECCEEEECCHHHHH
LAATIADLEAAH
HHHHHHHHHCCC
>Mature Secondary Structure
MIVFKLPDLGEGLQEAEIVQWHVQAGDTVEADQPLVSVETAKAIVEIPSPQAGRIARLFG
CEEEECCCCCCCCHHHHEEEEEECCCCCCCCCCCEEEHHHHHHEEECCCCCCCHHHHHHC
QPGDIVHLGAPLVAFEGAGGGEADAGTVVGQMQVGQQVVREAPAVLAAAPGAAAGGIKAT
CCCCEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCC
PAVRALARKLDVDLSMATPSGPDGVITAADVERVAKVFSELGPPEVLRGVRRAMAQNMAR
HHHHHHHHHHCCCEEECCCCCCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
AQSEVAAATVIDDADIHAWPPGTDVTIRLIRALVAGCRAEPGLNAWFDGHAGRRHVLEKI
HHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
DLGIAVDLPDGLFVPVLRNVAHRDAADLRAGLDRMRADIRARKIPPEELRGNTITLSNFG
CCCEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCEEEEECCC
MIAGKYAAPVVVPPTVAILGAGRIHEQVVAAAGAPAVHRVLPLSLTFDHRVVTGGEAARF
EEECCCCCCEECCCHHEEECCCHHHHHHHHHCCCCHHHHHEEEEEEECCEEEECCHHHHH
LAATIADLEAAH
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043; 3046941 [H]