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The map label for this gene is pncA [H]

Identifier: 17546479

GI number: 17546479

Start: 1886495

End: 1887127

Strand: Direct

Name: pncA [H]

Synonym: RSc1760

Alternate gene names: 17546479

Gene position: 1886495-1887127 (Clockwise)

Preceding gene: 17546478

Following gene: 17546480

Centisome position: 50.76

GC content: 70.46

Gene sequence:

>633_bases
ATGCATCCCACCGCCACCGATTGCCTGCTGGTCATGGACGTGCAGAACGACTTTCTGCCCGGCGGTGCGCTGGCCGTGCC
GGACGGCGACCGGGTGATCCCGGTCATCAACCGGCTGGCCCGGGCGTTCGGGCGCGTGGTGCTGACGCAGGACTGGCACC
CGCGCGACCACGTGTCGTTCGCCGCCAACCACCCCGGCGCGCAGCCGTTCGGCACGATCGACCTGCCGTACGGGCAGCAG
GTGCTGTGGCCCGTGCACTGCGTGCAGCACAGCACCGGCGCGGCGCTGGCCGACGCGCTGGACGTGCCGCATGCGCAGCT
GATCGTGCGCAAGGGCTATCACCGGCACATCGACAGCTACTCCGCCTTCTTCGAGGCGGACCGCACCACGCCCACCGGCT
TGCTCGGCTATCTGCGCGAGCTCGGGATCCGGCACGTGTTCTGCGCCGGGCTGGCCACGGACTTCTGCGTGGCATGGTCG
GCGCTGGATGCGCGGGCGGCGGGGCTGGAGGTGGTCGTCATCGAGGACGCCTGCCGTGCCATCGACCTGGACGGCTCGCT
GGCGCAGGCCTGGCAGCGCATGACGGATGCCGGCATCGCGCGATTGCAGTCGCGCGACGTCTTGAACGGATAA

Upstream 100 bases:

>100_bases
CGGCGAAGCCTGGAAGCCGGCGCCGCTGCTGCAGAAGCTGGCCGACGCCGGCCAGGGCTTCAACGGCTGAACGGCCGAGC
CACCCGCCACGGAGCACGCC

Downstream 100 bases:

>100_bases
CGGAATACCAGAGCGCGCCTGCGAACGCAGCGCGCCGCCACCAGATACGAGGAGCACCACCATGACCGAAGCAGTGATCG
TTTCCACCGCACGTACGGGC

Product: bifunctional pyrazinamidase/nicotinamidase hydrolase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MHPTATDCLLVMDVQNDFLPGGALAVPDGDRVIPVINRLARAFGRVVLTQDWHPRDHVSFAANHPGAQPFGTIDLPYGQQ
VLWPVHCVQHSTGAALADALDVPHAQLIVRKGYHRHIDSYSAFFEADRTTPTGLLGYLRELGIRHVFCAGLATDFCVAWS
ALDARAAGLEVVVIEDACRAIDLDGSLAQAWQRMTDAGIARLQSRDVLNG

Sequences:

>Translated_210_residues
MHPTATDCLLVMDVQNDFLPGGALAVPDGDRVIPVINRLARAFGRVVLTQDWHPRDHVSFAANHPGAQPFGTIDLPYGQQ
VLWPVHCVQHSTGAALADALDVPHAQLIVRKGYHRHIDSYSAFFEADRTTPTGLLGYLRELGIRHVFCAGLATDFCVAWS
ALDARAAGLEVVVIEDACRAIDLDGSLAQAWQRMTDAGIARLQSRDVLNG
>Mature_210_residues
MHPTATDCLLVMDVQNDFLPGGALAVPDGDRVIPVINRLARAFGRVVLTQDWHPRDHVSFAANHPGAQPFGTIDLPYGQQ
VLWPVHCVQHSTGAALADALDVPHAQLIVRKGYHRHIDSYSAFFEADRTTPTGLLGYLRELGIRHVFCAGLATDFCVAWS
ALDARAAGLEVVVIEDACRAIDLDGSLAQAWQRMTDAGIARLQSRDVLNG

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=195, Percent_Identity=43.0769230769231, Blast_Score=143, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI193206735, Length=228, Percent_Identity=30.2631578947368, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17543130, Length=228, Percent_Identity=30.2631578947368, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71999339, Length=232, Percent_Identity=29.7413793103448, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321401, Length=193, Percent_Identity=35.2331606217617, Blast_Score=114, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24648247, Length=200, Percent_Identity=35.5, Blast_Score=113, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 22826; Mature: 22826

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPTATDCLLVMDVQNDFLPGGALAVPDGDRVIPVINRLARAFGRVVLTQDWHPRDHVSF
CCCCCCCEEEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCCEE
AANHPGAQPFGTIDLPYGQQVLWPVHCVQHSTGAALADALDVPHAQLIVRKGYHRHIDSY
ECCCCCCCCCCEEECCCCCCHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHCCHHHHHHHH
SAFFEADRTTPTGLLGYLRELGIRHVFCAGLATDFCVAWSALDARAAGLEVVVIEDACRA
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCE
IDLDGSLAQAWQRMTDAGIARLQSRDVLNG
ECCCCHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MHPTATDCLLVMDVQNDFLPGGALAVPDGDRVIPVINRLARAFGRVVLTQDWHPRDHVSF
CCCCCCCEEEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCCEE
AANHPGAQPFGTIDLPYGQQVLWPVHCVQHSTGAALADALDVPHAQLIVRKGYHRHIDSY
ECCCCCCCCCCEEECCCCCCHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHCCHHHHHHHH
SAFFEADRTTPTGLLGYLRELGIRHVFCAGLATDFCVAWSALDARAAGLEVVVIEDACRA
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCE
IDLDGSLAQAWQRMTDAGIARLQSRDVLNG
ECCCCHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]