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The map label for this gene is livJ [C]

Identifier: 17546469

GI number: 17546469

Start: 1872682

End: 1873935

Strand: Direct

Name: livJ [C]

Synonym: RSc1750

Alternate gene names: 17546469

Gene position: 1872682-1873935 (Clockwise)

Preceding gene: 17546468

Following gene: 17546470

Centisome position: 50.39

GC content: 65.15

Gene sequence:

>1254_bases
ATGACGAAGTTCCGTCCGTTGATCGTGGCCGTTGCAAGTGTCGCGGCAATCGGTGGAGGGCTGGCAAGCGCCGCCGCATC
CGCCGCCGAGACCGTCAAGATCGCCTGGATCGATCCGCTCTCCGGCCTGATGGGCGCGCTGGGCCAGAATCAGCTGCGCA
GCTGGCAGTACATCGCCGAGCTGGCCACGCAGAAGAACTGGGCCGGCGACGGCACCCGGTTCGAGGTGGTGGGCTTCGAC
AACAAGCTCTCGCCGCAGGAGAGCCTGACGGTGCTCAAGCAGGTGGCCGACCAGGGCATCCACTACATCGCGCAGGGCAA
CGGCTCCAGCGTGGGCATGGCGCTGGAGGACGCGGTCGCCAAGTACAACGAGCGCAACCCGGGCAAGGAGATCGTCTACC
TGAACTACGCGGCGGTCGACCCGGACATGACCAACGGCAAGTGCAACTACTGGCACTTCCGCCTGGACGCGAACTCCGAC
ATGAAGATGGAGGCCCTGACCAGCTACCTGGCCAAGGACCCGGGCGTCAAGAAGGTCTACCTGATCAACCAGAACTACTC
GTTCGGCCACCAGGTGGCGCGCGCGGCCAAGGCGTATCTCAAGCGCAAGCGCCCCGACATCGAGATCGTCGGCGAAGACC
TGCATCCGCTGGCGCAGGTGAAGGATTTCTCGCCGTACGTGTCGAAGATCAAAGCGTCGGGCGCCGATACCGTCATCACC
GGCAACTGGGGCAGCGACCTGGCGCTGCTGATCAAGGCCGGCAAGGATGCGGGCCTGAACGCCAATTTCTACACCTACTA
CGCCTCGAGCACCGGCGTGCCGACCGCCATGGGCCCCGCCGGCGCCGACCACGTCAAGTACGTCGGCTACTGGAACGTCA
ACAACGACGGCTTCAAGGGCGCCGACATCGTCGAGGGCTACAAGAAGAAGTACAACGACGACTACTACCTGATGGCGTCG
TACACCGGCATCGCGCTGCTGGCCAAGGCCGTCAAGCAGGCCCGGTCCGCCGAGCCGGGCAAGGTCGCCCGGGCCCTGGA
GGGGATGAAGGTCGACAGCCTGAACGGCCCGTTCGAGATGCGCGCCTCCGACCACCAGGGCCAGCAGCCGCTCTACATCG
CCACCTGGAAGAAGACCGACGGCAAGACCGTGCGCTTCGACCAGGAGAACACCGGCTACGGCTGGAGGACCGATGCGGTG
CTCGACCAGTACATCGCGTCGCAGCCGACCTCCTGCCAGATGAAGCGCCCCTGA

Upstream 100 bases:

>100_bases
GGCGCGTTGCAACCTGGAGACGTCAAGAGCCGGCGTGCCCGCAGCGGTGGCGCGCCGGAGAGTGACTCGTCGGCGACTCC
ACCACCTGAAGGAGACACGC

Downstream 100 bases:

>100_bases
CCTGAAATCAGACGGCGGGTGGTGTGCCGAACGGCCGCCCGATGCTATCGTGCGGCGATGTTTGAAACGGGCGGGGGCAT
GGGCCGGCACCGTTGGCCTA

Product: Leu/Ile/Val-binding precursor signal peptide protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MTKFRPLIVAVASVAAIGGGLASAAASAAETVKIAWIDPLSGLMGALGQNQLRSWQYIAELATQKNWAGDGTRFEVVGFD
NKLSPQESLTVLKQVADQGIHYIAQGNGSSVGMALEDAVAKYNERNPGKEIVYLNYAAVDPDMTNGKCNYWHFRLDANSD
MKMEALTSYLAKDPGVKKVYLINQNYSFGHQVARAAKAYLKRKRPDIEIVGEDLHPLAQVKDFSPYVSKIKASGADTVIT
GNWGSDLALLIKAGKDAGLNANFYTYYASSTGVPTAMGPAGADHVKYVGYWNVNNDGFKGADIVEGYKKKYNDDYYLMAS
YTGIALLAKAVKQARSAEPGKVARALEGMKVDSLNGPFEMRASDHQGQQPLYIATWKKTDGKTVRFDQENTGYGWRTDAV
LDQYIASQPTSCQMKRP

Sequences:

>Translated_417_residues
MTKFRPLIVAVASVAAIGGGLASAAASAAETVKIAWIDPLSGLMGALGQNQLRSWQYIAELATQKNWAGDGTRFEVVGFD
NKLSPQESLTVLKQVADQGIHYIAQGNGSSVGMALEDAVAKYNERNPGKEIVYLNYAAVDPDMTNGKCNYWHFRLDANSD
MKMEALTSYLAKDPGVKKVYLINQNYSFGHQVARAAKAYLKRKRPDIEIVGEDLHPLAQVKDFSPYVSKIKASGADTVIT
GNWGSDLALLIKAGKDAGLNANFYTYYASSTGVPTAMGPAGADHVKYVGYWNVNNDGFKGADIVEGYKKKYNDDYYLMAS
YTGIALLAKAVKQARSAEPGKVARALEGMKVDSLNGPFEMRASDHQGQQPLYIATWKKTDGKTVRFDQENTGYGWRTDAV
LDQYIASQPTSCQMKRP
>Mature_416_residues
TKFRPLIVAVASVAAIGGGLASAAASAAETVKIAWIDPLSGLMGALGQNQLRSWQYIAELATQKNWAGDGTRFEVVGFDN
KLSPQESLTVLKQVADQGIHYIAQGNGSSVGMALEDAVAKYNERNPGKEIVYLNYAAVDPDMTNGKCNYWHFRLDANSDM
KMEALTSYLAKDPGVKKVYLINQNYSFGHQVARAAKAYLKRKRPDIEIVGEDLHPLAQVKDFSPYVSKIKASGADTVITG
NWGSDLALLIKAGKDAGLNANFYTYYASSTGVPTAMGPAGADHVKYVGYWNVNNDGFKGADIVEGYKKKYNDDYYLMASY
TGIALLAKAVKQARSAEPGKVARALEGMKVDSLNGPFEMRASDHQGQQPLYIATWKKTDGKTVRFDQENTGYGWRTDAVL
DQYIASQPTSCQMKRP

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=353, Percent_Identity=20.9631728045326, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000709 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 45466; Mature: 45335

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: PS00995 TCP1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKFRPLIVAVASVAAIGGGLASAAASAAETVKIAWIDPLSGLMGALGQNQLRSWQYIAE
CCCCHHHHHHHHHHHHHCCHHHHHHHHHHHEEEEEEECCHHHHHHHCCHHHHHHHHHHHH
LATQKNWAGDGTRFEVVGFDNKLSPQESLTVLKQVADQGIHYIAQGNGSSVGMALEDAVA
HHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHH
KYNERNPGKEIVYLNYAAVDPDMTNGKCNYWHFRLDANSDMKMEALTSYLAKDPGVKKVY
HHCCCCCCCEEEEEEEEEECCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEE
LINQNYSFGHQVARAAKAYLKRKRPDIEIVGEDLHPLAQVKDFSPYVSKIKASGADTVIT
EEECCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCCHHHHHHHCCCCCEEEE
GNWGSDLALLIKAGKDAGLNANFYTYYASSTGVPTAMGPAGADHVKYVGYWNVNNDGFKG
CCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCCCC
ADIVEGYKKKYNDDYYLMASYTGIALLAKAVKQARSAEPGKVARALEGMKVDSLNGPFEM
HHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEECCCCCEEE
RASDHQGQQPLYIATWKKTDGKTVRFDQENTGYGWRTDAVLDQYIASQPTSCQMKRP
CCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCEEHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TKFRPLIVAVASVAAIGGGLASAAASAAETVKIAWIDPLSGLMGALGQNQLRSWQYIAE
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHEEEEEEECCHHHHHHHCCHHHHHHHHHHHH
LATQKNWAGDGTRFEVVGFDNKLSPQESLTVLKQVADQGIHYIAQGNGSSVGMALEDAVA
HHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHH
KYNERNPGKEIVYLNYAAVDPDMTNGKCNYWHFRLDANSDMKMEALTSYLAKDPGVKKVY
HHCCCCCCCEEEEEEEEEECCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEE
LINQNYSFGHQVARAAKAYLKRKRPDIEIVGEDLHPLAQVKDFSPYVSKIKASGADTVIT
EEECCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCCHHHHHHHCCCCCEEEE
GNWGSDLALLIKAGKDAGLNANFYTYYASSTGVPTAMGPAGADHVKYVGYWNVNNDGFKG
CCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCCCC
ADIVEGYKKKYNDDYYLMASYTGIALLAKAVKQARSAEPGKVARALEGMKVDSLNGPFEM
HHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEECCCCCEEE
RASDHQGQQPLYIATWKKTDGKTVRFDQENTGYGWRTDAVLDQYIASQPTSCQMKRP
CCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCEEHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA