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The map label for this gene is ispD

Identifier: 17546362

GI number: 17546362

Start: 1767907

End: 1768668

Strand: Direct

Name: ispD

Synonym: RSc1643

Alternate gene names: 17546362

Gene position: 1767907-1768668 (Clockwise)

Preceding gene: 17546358

Following gene: 17546363

Centisome position: 47.57

GC content: 73.49

Gene sequence:

>762_bases
ATGTCCGTTTCCTCCCGTCCCGGCCGACGCCGGTTTGCGCTGATCCCGTCCGCCGGCACCGGCACGCGTGCCGGCGGCGA
TCTGCCCAAGCAGTACCAGCCCCTCGCTGGCCGCCCGATGCTGTGGCATACCGTGCAGGCCTTCCTGGCCAGCGCGGCGA
TCGACCGGGTGGTGGTGGTGACTTCGCCGGGCGCGCCGGCGCTGGCGGCGCGCTTTCCCGGCCTGGGTTTCGACGCGGCC
CGGCTGGTGGAGGTCGATTGCGGCGGCGATTCGCGCCATGCCTCGGTCACCAACGGCCTGGCGGCGCTGCGTGGCCTCGG
CGCGCACGAAGACGACTGGGTGCTCGTGCACGATGCCGCCCGCCCGGGCCTCACGCCTGCCCTGATCGCGCGGCTGGTCG
GCGCCGTCGAGGCCGACGGCGGGCAGGCGGTCGGCGGCATCCTGGCGCTGCCGGTAGCCGATACGCTCAAGCGCGCCGAT
GCCGGGCAGCACATCGCCGAGACCGTGCCGCGCGGCGGCCTGTGGCAGGCGCAGACGCCGCAGATGTTTCCGCTCGGCCT
GCTGCAGCGCGCCCTGTCGGAGGCCCTGACCGCGCAGCGCATCGTCACCGATGAGGCCAGCGCCATCGAGGCCGCCGGTC
ACCGGCCCTTGCTGGTGGCCGGCGCGCTGCGCAACTTCAAGGTGACTTATCCCGACGATTTCGCGCTCGCCGAGGCCATC
CTCGCGGGCGCCGCACCGACCCAGGAGGATTCGCAAGCATGA

Upstream 100 bases:

>100_bases
GGTACTGGCCGCTATTATAAAATCCGCGCCAATCGCGCGCTGGCCGACGGGAAATTTCCCGACCAAAAGGCTGCGCTCAC
GCCATTTTTCCGCTTGCCGC

Downstream 100 bases:

>100_bases
ACTGGATGGACATCCGCATCGGCCAGGGCTACGACGTGCACGCGCTGGTGGAGGGCCGCAAGCTGATCCTGGGCGGCGTG
GAGATTCCGCACACGCGCGG

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MSVSSRPGRRRFALIPSAGTGTRAGGDLPKQYQPLAGRPMLWHTVQAFLASAAIDRVVVVTSPGAPALAARFPGLGFDAA
RLVEVDCGGDSRHASVTNGLAALRGLGAHEDDWVLVHDAARPGLTPALIARLVGAVEADGGQAVGGILALPVADTLKRAD
AGQHIAETVPRGGLWQAQTPQMFPLGLLQRALSEALTAQRIVTDEASAIEAAGHRPLLVAGALRNFKVTYPDDFALAEAI
LAGAAPTQEDSQA

Sequences:

>Translated_253_residues
MSVSSRPGRRRFALIPSAGTGTRAGGDLPKQYQPLAGRPMLWHTVQAFLASAAIDRVVVVTSPGAPALAARFPGLGFDAA
RLVEVDCGGDSRHASVTNGLAALRGLGAHEDDWVLVHDAARPGLTPALIARLVGAVEADGGQAVGGILALPVADTLKRAD
AGQHIAETVPRGGLWQAQTPQMFPLGLLQRALSEALTAQRIVTDEASAIEAAGHRPLLVAGALRNFKVTYPDDFALAEAI
LAGAAPTQEDSQA
>Mature_252_residues
SVSSRPGRRRFALIPSAGTGTRAGGDLPKQYQPLAGRPMLWHTVQAFLASAAIDRVVVVTSPGAPALAARFPGLGFDAAR
LVEVDCGGDSRHASVTNGLAALRGLGAHEDDWVLVHDAARPGLTPALIARLVGAVEADGGQAVGGILALPVADTLKRADA
GQHIAETVPRGGLWQAQTPQMFPLGLLQRALSEALTAQRIVTDEASAIEAAGHRPLLVAGALRNFKVTYPDDFALAEAIL
AGAAPTQEDSQA

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=247, Percent_Identity=27.9352226720648, Blast_Score=75, Evalue=8e-14,
Organism=Escherichia coli, GI1789104, Length=230, Percent_Identity=46.0869565217391, Blast_Score=166, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_RALSO (Q8XYW3)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_519764.1
- ProteinModelPortal:   Q8XYW3
- SMR:   Q8XYW3
- GeneID:   1220478
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc1643
- NMPDR:   fig|267608.1.peg.1643
- HOGENOM:   HBG672839
- OMA:   FPQGAIL
- ProtClustDB:   PRK00155
- BioCyc:   RSOL267608:RSC1643-MONOMER
- BRENDA:   2.7.7.60
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 26134; Mature: 26003

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSSRPGRRRFALIPSAGTGTRAGGDLPKQYQPLAGRPMLWHTVQAFLASAAIDRVVVV
CCCCCCCCCCEEEEECCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHCEEEEE
TSPGAPALAARFPGLGFDAARLVEVDCGGDSRHASVTNGLAALRGLGAHEDDWVLVHDAA
ECCCCCHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCC
RPGLTPALIARLVGAVEADGGQAVGGILALPVADTLKRADAGQHIAETVPRGGLWQAQTP
CCCCCHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHCCCHHHHHHCCCCCCCCCCCC
QMFPLGLLQRALSEALTAQRIVTDEASAIEAAGHRPLLVAGALRNFKVTYPDDFALAEAI
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEECCCCCEEECCCHHHHHHHH
LAGAAPTQEDSQA
HHCCCCCCCCCCC
>Mature Secondary Structure 
SVSSRPGRRRFALIPSAGTGTRAGGDLPKQYQPLAGRPMLWHTVQAFLASAAIDRVVVV
CCCCCCCCCEEEEECCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHCEEEEE
TSPGAPALAARFPGLGFDAARLVEVDCGGDSRHASVTNGLAALRGLGAHEDDWVLVHDAA
ECCCCCHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCC
RPGLTPALIARLVGAVEADGGQAVGGILALPVADTLKRADAGQHIAETVPRGGLWQAQTP
CCCCCHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHCCCHHHHHHCCCCCCCCCCCC
QMFPLGLLQRALSEALTAQRIVTDEASAIEAAGHRPLLVAGALRNFKVTYPDDFALAEAI
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEECCCCCEEECCCHHHHHHHH
LAGAAPTQEDSQA
HHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852