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The map label for this gene is phbC [H]

Identifier: 17546350

GI number: 17546350

Start: 1751336

End: 1753132

Strand: Direct

Name: phbC [H]

Synonym: RSc1631

Alternate gene names: 17546350

Gene position: 1751336-1753132 (Clockwise)

Preceding gene: 17546348

Following gene: 17546351

Centisome position: 47.12

GC content: 67.28

Gene sequence:

>1797_bases
ATGGCATCGCGTCACAAGGCATCTTCCCATGCAACCGGATCCCAGGCCCAGCCAGCGTGGCATGGCTTGGCCGATCCTGC
CCAGTGGGCGGAGCAGTTCAGGCAGTGGCAGTCCATGGCGGGGGCGTTCGCGCAGTCCGGCGCACCGGGCTCCACCGGGT
TCGGACCGGCTGGCGGCATGCCGACGGGCGAGGGCGCGATGCCGTTTGCGCTGATCCCGCCCGAGCGGCTGGCCGAGATC
CAGCAGAAGTACCTCGAAGCGTGGCTCCAGATCTGGGGGCACATGAGCGCCGGCGACAGCGCCGAGACCGTGGCTCCCTC
CGACCGCCGCTTTGCCAACGACGCTTGGCGCAAGAGTCCGCTGTATGGCTATGCCGCGGCCTTCTATGTCCTCAATGCGC
GCACGCTGATGGACATGGCCGATGCCGTGCAGGCCGATGCCAAGACGCGCGAGCGCGTGCGCTTTGCCGTGTCGCAATGG
ACCGCCGCCATGTCGCCGTCCAACTTCCTGGCGACCAACCCCGAGGCGCAGAAGCAACTGATCGAGTCGCGCGGCGAGTC
GCTGCGCGCCGGCATCCTCAACATGCTGCAGGACATGGAACGCGGCAAGATCTCGCAGACGGACGAGACCGCGTTCGAGA
TCGGCCGCAACGTCGCCAACAGCGAAGGTGCGGTGGTCTTCGAGAACGACTACTTCCAGCTGATCCAGTACAAGCCGCTG
ACGGCCAAGGTGCATGCCCGCCCGCTGCTGCTGGTGCCGCCCTGTATCAACAAGTACTACATCCTGGATCTGCAGCCGGC
CAACTCGCTGGTGCGCTACTCGGTGGAGCAGGGGCATACGGTGTTCCTGGTGTCGTGGCGCAACCCGGATGCCAGCATGG
CCGCGCGCAACTGGGATGACTACATCGAGGGCGGCGCGATCGAGGCCATCCGGGTGGCCCGCGAGATTGCCGCGCAGGAC
CGGATCAACGTGCTCGGCTTCTGCGTGGGCGGCACGATCCTCTCCACGGCGTTGGCGGTGCTGGCCGCGCGCGGCCAGCA
TCCGGCGGCCAGCCTGACCCTGCTGACCACGCTGCTGGATTTCAGCGACACCGGCATCCTCGACGTGTTCGTGGACCAGG
CCCAGGTGGAGATGCGCGAGCAGACCATCGGCAGCGCGGCCCCGGCCGGCCCCGGCCTGCTGCGCGGCGTGGAGCTGGCG
AACACGTTCTCCTTCCTGCGTCCGAACGACCTGGTGTGGAACTACGTGGTCGAGAACTACCTGAAGGGCAAGACGCCCGC
GCCGTTCGACCTGCTGTACTGGAACGGCGATTCCACCAATCTGCCCGGCCCGTGGTACTGCTGGTACCTGCGCCACACGT
ACCTGCAGAACGACCTGATGGCGCCGGGCAGGCTGACGGTCTGCGGCGAGCCGGTCGATCTCGGCCGGATCGAGGTGCCC
GTCTATCTCTACGGTTCGCGCGAAGATCACATCGTCCCCTGGAAATCCGCCTATGCTTCGACGCAGCTGCTTAAGGGCGA
GCGGCGTTTTGTGCTGGGCGCCTCGGGGCACATCGCGGGCGTCATCAACCCGCCGGCGGCCAATAAGCGCGCGCACTGGA
TCAACGCCGGGCTACCGGATTCGGCCCAGGCCTGGCTGGACGGCGCGAAGGAGCATCCGGGCAGCTGGTGGCCGGACTGG
TCCGCGTGGCTGGCCAAGCACGCCGGGGCGCAGAAGGCCGCTCCCAAGCACTACGGCAATGCGGACCATCCGGTGATCGA
GCCGGCACCGGGCCGCTACGTCAAACAGAAAGCGTAA

Upstream 100 bases:

>100_bases
ATTGACAGCCGTGAAATCGCGAGGCAACAATGCCCCGCAATACTGCGACGGACGCCCTATATTCAGCCGGGTCGGCATCG
TCGCCCGCATCGAGGACACT

Downstream 100 bases:

>100_bases
TAGCGCGCGCGCCACGCCGTGGCGGATTGTGCGCGGACAGCGCTTCCCCCAACCTTTTTGAGATCGTGGAAGGAAAGCAA
ATGACCGATGTAGTGATCGT

Product: poly-beta-hydroxybutyrate polymerase transmembrane protein

Products: NA

Alternate protein names: PHA synthase; PHB synthase; Poly(3-hydroxyalkanoate) polymerase; PHA polymerase; Poly(3-hydroxybutyrate) polymerase; PHB polymerase; Polyhydroxyalkanoic acid synthase [H]

Number of amino acids: Translated: 598; Mature: 597

Protein sequence:

>598_residues
MASRHKASSHATGSQAQPAWHGLADPAQWAEQFRQWQSMAGAFAQSGAPGSTGFGPAGGMPTGEGAMPFALIPPERLAEI
QQKYLEAWLQIWGHMSAGDSAETVAPSDRRFANDAWRKSPLYGYAAAFYVLNARTLMDMADAVQADAKTRERVRFAVSQW
TAAMSPSNFLATNPEAQKQLIESRGESLRAGILNMLQDMERGKISQTDETAFEIGRNVANSEGAVVFENDYFQLIQYKPL
TAKVHARPLLLVPPCINKYYILDLQPANSLVRYSVEQGHTVFLVSWRNPDASMAARNWDDYIEGGAIEAIRVAREIAAQD
RINVLGFCVGGTILSTALAVLAARGQHPAASLTLLTTLLDFSDTGILDVFVDQAQVEMREQTIGSAAPAGPGLLRGVELA
NTFSFLRPNDLVWNYVVENYLKGKTPAPFDLLYWNGDSTNLPGPWYCWYLRHTYLQNDLMAPGRLTVCGEPVDLGRIEVP
VYLYGSREDHIVPWKSAYASTQLLKGERRFVLGASGHIAGVINPPAANKRAHWINAGLPDSAQAWLDGAKEHPGSWWPDW
SAWLAKHAGAQKAAPKHYGNADHPVIEPAPGRYVKQKA

Sequences:

>Translated_598_residues
MASRHKASSHATGSQAQPAWHGLADPAQWAEQFRQWQSMAGAFAQSGAPGSTGFGPAGGMPTGEGAMPFALIPPERLAEI
QQKYLEAWLQIWGHMSAGDSAETVAPSDRRFANDAWRKSPLYGYAAAFYVLNARTLMDMADAVQADAKTRERVRFAVSQW
TAAMSPSNFLATNPEAQKQLIESRGESLRAGILNMLQDMERGKISQTDETAFEIGRNVANSEGAVVFENDYFQLIQYKPL
TAKVHARPLLLVPPCINKYYILDLQPANSLVRYSVEQGHTVFLVSWRNPDASMAARNWDDYIEGGAIEAIRVAREIAAQD
RINVLGFCVGGTILSTALAVLAARGQHPAASLTLLTTLLDFSDTGILDVFVDQAQVEMREQTIGSAAPAGPGLLRGVELA
NTFSFLRPNDLVWNYVVENYLKGKTPAPFDLLYWNGDSTNLPGPWYCWYLRHTYLQNDLMAPGRLTVCGEPVDLGRIEVP
VYLYGSREDHIVPWKSAYASTQLLKGERRFVLGASGHIAGVINPPAANKRAHWINAGLPDSAQAWLDGAKEHPGSWWPDW
SAWLAKHAGAQKAAPKHYGNADHPVIEPAPGRYVKQKA
>Mature_597_residues
ASRHKASSHATGSQAQPAWHGLADPAQWAEQFRQWQSMAGAFAQSGAPGSTGFGPAGGMPTGEGAMPFALIPPERLAEIQ
QKYLEAWLQIWGHMSAGDSAETVAPSDRRFANDAWRKSPLYGYAAAFYVLNARTLMDMADAVQADAKTRERVRFAVSQWT
AAMSPSNFLATNPEAQKQLIESRGESLRAGILNMLQDMERGKISQTDETAFEIGRNVANSEGAVVFENDYFQLIQYKPLT
AKVHARPLLLVPPCINKYYILDLQPANSLVRYSVEQGHTVFLVSWRNPDASMAARNWDDYIEGGAIEAIRVAREIAAQDR
INVLGFCVGGTILSTALAVLAARGQHPAASLTLLTTLLDFSDTGILDVFVDQAQVEMREQTIGSAAPAGPGLLRGVELAN
TFSFLRPNDLVWNYVVENYLKGKTPAPFDLLYWNGDSTNLPGPWYCWYLRHTYLQNDLMAPGRLTVCGEPVDLGRIEVPV
YLYGSREDHIVPWKSAYASTQLLKGERRFVLGASGHIAGVINPPAANKRAHWINAGLPDSAQAWLDGAKEHPGSWWPDWS
AWLAKHAGAQKAAPKHYGNADHPVIEPAPGRYVKQKA

Specific function: Polymerizes d(-)-3-hydroxybutyryl-CoA to create PHB which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation [H]

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010963
- InterPro:   IPR010941 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF07167 PhaC_N [H]

EC number: NA

Molecular weight: Translated: 65608; Mature: 65476

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASRHKASSHATGSQAQPAWHGLADPAQWAEQFRQWQSMAGAFAQSGAPGSTGFGPAGGM
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PTGEGAMPFALIPPERLAEIQQKYLEAWLQIWGHMSAGDSAETVAPSDRRFANDAWRKSP
CCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCC
LYGYAAAFYVLNARTLMDMADAVQADAKTRERVRFAVSQWTAAMSPSNFLATNPEAQKQL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHH
IESRGESLRAGILNMLQDMERGKISQTDETAFEIGRNVANSEGAVVFENDYFQLIQYKPL
HHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCEEEEEECCC
TAKVHARPLLLVPPCINKYYILDLQPANSLVRYSVEQGHTVFLVSWRNPDASMAARNWDD
EEEECCCEEEEECCCCCCEEEEEECCCCHHHHHHHCCCCEEEEEEECCCCHHHHHCCCHH
YIEGGAIEAIRVAREIAAQDRINVLGFCVGGTILSTALAVLAARGQHPAASLTLLTTLLD
HHCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
FSDTGILDVFVDQAQVEMREQTIGSAAPAGPGLLRGVELANTFSFLRPNDLVWNYVVENY
CCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LKGKTPAPFDLLYWNGDSTNLPGPWYCWYLRHTYLQNDLMAPGRLTVCGEPVDLGRIEVP
HCCCCCCCEEEEEECCCCCCCCCCEEEHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEE
VYLYGSREDHIVPWKSAYASTQLLKGERRFVLGASGHIAGVINPPAANKRAHWINAGLPD
EEEECCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCCCCCEEEECCCCC
SAQAWLDGAKEHPGSWWPDWSAWLAKHAGAQKAAPKHYGNADHPVIEPAPGRYVKQKA
HHHHHHCCHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCCCCCCCC
>Mature Secondary Structure 
ASRHKASSHATGSQAQPAWHGLADPAQWAEQFRQWQSMAGAFAQSGAPGSTGFGPAGGM
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PTGEGAMPFALIPPERLAEIQQKYLEAWLQIWGHMSAGDSAETVAPSDRRFANDAWRKSP
CCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCC
LYGYAAAFYVLNARTLMDMADAVQADAKTRERVRFAVSQWTAAMSPSNFLATNPEAQKQL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHH
IESRGESLRAGILNMLQDMERGKISQTDETAFEIGRNVANSEGAVVFENDYFQLIQYKPL
HHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCEEEEEECCC
TAKVHARPLLLVPPCINKYYILDLQPANSLVRYSVEQGHTVFLVSWRNPDASMAARNWDD
EEEECCCEEEEECCCCCCEEEEEECCCCHHHHHHHCCCCEEEEEEECCCCHHHHHCCCHH
YIEGGAIEAIRVAREIAAQDRINVLGFCVGGTILSTALAVLAARGQHPAASLTLLTTLLD
HHCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
FSDTGILDVFVDQAQVEMREQTIGSAAPAGPGLLRGVELANTFSFLRPNDLVWNYVVENY
CCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LKGKTPAPFDLLYWNGDSTNLPGPWYCWYLRHTYLQNDLMAPGRLTVCGEPVDLGRIEVP
HCCCCCCCEEEEEECCCCCCCCCCEEEHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEE
VYLYGSREDHIVPWKSAYASTQLLKGERRFVLGASGHIAGVINPPAANKRAHWINAGLPD
EEEECCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCCCCCEEEECCCCC
SAQAWLDGAKEHPGSWWPDWSAWLAKHAGAQKAAPKHYGNADHPVIEPAPGRYVKQKA
HHHHHHCCHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2670936; 1987116 [H]