Click here to switch to the map view.

The map label for this gene is aceF [H]

Identifier: 17546320

GI number: 17546320

Start: 1720157

End: 1721821

Strand: Direct

Name: aceF [H]

Synonym: RSc1601

Alternate gene names: 17546320

Gene position: 1720157-1721821 (Clockwise)

Preceding gene: 17546319

Following gene: 17546321

Centisome position: 46.29

GC content: 68.23

Gene sequence:

>1665_bases
ATGAGTCAAGTCGTAGAAATCAAGGTGCCGGACATCGGCGACTATAAGGACGTGCCGGTCATCGAGCTGCACGTCAAGGC
GGGCGATACCGTCAGCGCCGAGGATTCGCTGGTCACGCTGGAGTCGGACAAGGCGACCATGGATGTGCCGTCGCCCAAGA
GCGGCGTCGTCAAGGAAGTGAAGATCAAGGTGGGCGACGCCGTGTCGGAAGGCTCGCTGGTGCTGCTGCTCGAAGAGCAG
GGCGCCACGGCGGCTTCGGCGCCTCAGGCCGCGCCCGCGCCGGCCCCGGTGGCTGCGGCCCTCGCGCCGGCTGCACAGGC
TCCCGTGTCCGCGCCGGCGGCCCAGCCCGCGGCGGGCGGCGGCACGATCGAAGTGAAGGTGCCGGACATTGGCGACTACA
AGGATGTGCCGGTCATCGAGATCAGCGTGAAGGTGGGCGACAAGGTGGAAGCCGAGCAGTCGCTGATCACGCTCGAATCC
GACAAGGCCACGATGGATGTGCCGTCGCCGGCCGCCGGCACGGTCAAGGACATCCGCGTGAAGGTGGGCGACGCCGTGTC
CGAAGGCACGCTGATCGTCGTGCTGGAAGGCGCGGGCGGTGCGGCCGCAGCTGCGCCGGCTCCGGCGCCCGCGCCGGCGG
CTGCCGCCCCGAGCCCGGCGCCCGCTGTCGCACCCGTCGCAGCCCCGGCCGCCGCGCCTGCCACGTATACCGCCGACACC
GTCGGCACGATCGGCAAGGCCGCACACGCCAGCCCCTCGGTGCGCAAGTACGCGCGCGAGCTGGGTGTGGACGTGAACCT
GGTCGGCGGCACCGGCCCGAAGAACCGCATCACGCAGGAAGACGTGCAGCGCTACGTCAAGGGCGTGATGACCGGCCAGG
CAGCTGCGCCGGGCAAGGCCGCCGCTGCGGCGGCACCGGCCGGCGGCGGCGAACTGAACCTGCTGCCGTGGCCGAAGGTC
GACTTCACCAAGTTCGGCCCGGTCGAGCCCAAGCCGCTGTCGCGCATCAAGAAGATCTCCGGCGCGAACCTGCACCGCAA
CTGGGTCATGATCCCGCACGTCACCAACAATGACGAAGCGGACATCACCGAGCTGGAAGCCTTCCGCGTGCAGATGAACA
AGGAGCACGAAAAGGCCGGCGTGAAGTTCACCATGCTGGCGTTCGTGATCAAGGCGGTCGTGGGCGCGCTCAAGAAGTTC
CCGACCTTCAACGCCAGCCTGGACGGCGACAACCTGGTCTTCAAGCAGTATTACCACATTGGCTTCGCCGCCGACACGCC
CAACGGGCTGGTGGTGCCGGTGATCCGCGATGCCGACAAAAAGGGCGTGGTGGACATCGCCCGCGAAATGGCCGAGCTGT
CCAAGGCCGCGCGCGAAGGCAAGCTCAAGCCGGACCAGATGCAGGGCGGTTGCTTCTCGATCTCGTCGCTGGGCGGCATC
GGCGGCACGCACTTCACGCCGATCATCAATGCGCCGGAAGTGGCCATCCTGGGCCTGTCGCGCGGCTATCAGAAGCCGGT
GTGGGACGGCAAGCAGTTCGTGCCGCGCCTGACGCTGCCGCTGTCGCTGTCGTACGATCACCGCGTGATCGACGGCGCGG
AGGCCGCACGCTTCAACGCGTACTTGGCGGCCGTGCTGGCGGACTTCCGCCGCGTGCTGCTGTAA

Upstream 100 bases:

>100_bases
CTGCATAGTGCATTTGACGGCACGATGCGGTAGCCTGCCGGGCGACCGCGTGTCCGCCGCGCAGGCGTGCAGCGCCGCCA
GCCCCTCTGGAGACAGAAGA

Downstream 100 bases:

>100_bases
GCCGCTGGCGGCATGGCACGCATCCGGCTGCGGGCCGGGGCGTGCCGGCCCATGGGCAGAAGGCGGCCGGCGGACTTTTC
GCGAGGACGACATGACGACG

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 554; Mature: 553

Protein sequence:

>554_residues
MSQVVEIKVPDIGDYKDVPVIELHVKAGDTVSAEDSLVTLESDKATMDVPSPKSGVVKEVKIKVGDAVSEGSLVLLLEEQ
GATAASAPQAAPAPAPVAAALAPAAQAPVSAPAAQPAAGGGTIEVKVPDIGDYKDVPVIEISVKVGDKVEAEQSLITLES
DKATMDVPSPAAGTVKDIRVKVGDAVSEGTLIVVLEGAGGAAAAAPAPAPAPAAAAPSPAPAVAPVAAPAAAPATYTADT
VGTIGKAAHASPSVRKYARELGVDVNLVGGTGPKNRITQEDVQRYVKGVMTGQAAAPGKAAAAAAPAGGGELNLLPWPKV
DFTKFGPVEPKPLSRIKKISGANLHRNWVMIPHVTNNDEADITELEAFRVQMNKEHEKAGVKFTMLAFVIKAVVGALKKF
PTFNASLDGDNLVFKQYYHIGFAADTPNGLVVPVIRDADKKGVVDIAREMAELSKAAREGKLKPDQMQGGCFSISSLGGI
GGTHFTPIINAPEVAILGLSRGYQKPVWDGKQFVPRLTLPLSLSYDHRVIDGAEAARFNAYLAAVLADFRRVLL

Sequences:

>Translated_554_residues
MSQVVEIKVPDIGDYKDVPVIELHVKAGDTVSAEDSLVTLESDKATMDVPSPKSGVVKEVKIKVGDAVSEGSLVLLLEEQ
GATAASAPQAAPAPAPVAAALAPAAQAPVSAPAAQPAAGGGTIEVKVPDIGDYKDVPVIEISVKVGDKVEAEQSLITLES
DKATMDVPSPAAGTVKDIRVKVGDAVSEGTLIVVLEGAGGAAAAAPAPAPAPAAAAPSPAPAVAPVAAPAAAPATYTADT
VGTIGKAAHASPSVRKYARELGVDVNLVGGTGPKNRITQEDVQRYVKGVMTGQAAAPGKAAAAAAPAGGGELNLLPWPKV
DFTKFGPVEPKPLSRIKKISGANLHRNWVMIPHVTNNDEADITELEAFRVQMNKEHEKAGVKFTMLAFVIKAVVGALKKF
PTFNASLDGDNLVFKQYYHIGFAADTPNGLVVPVIRDADKKGVVDIAREMAELSKAAREGKLKPDQMQGGCFSISSLGGI
GGTHFTPIINAPEVAILGLSRGYQKPVWDGKQFVPRLTLPLSLSYDHRVIDGAEAARFNAYLAAVLADFRRVLL
>Mature_553_residues
SQVVEIKVPDIGDYKDVPVIELHVKAGDTVSAEDSLVTLESDKATMDVPSPKSGVVKEVKIKVGDAVSEGSLVLLLEEQG
ATAASAPQAAPAPAPVAAALAPAAQAPVSAPAAQPAAGGGTIEVKVPDIGDYKDVPVIEISVKVGDKVEAEQSLITLESD
KATMDVPSPAAGTVKDIRVKVGDAVSEGTLIVVLEGAGGAAAAAPAPAPAPAAAAPSPAPAVAPVAAPAAAPATYTADTV
GTIGKAAHASPSVRKYARELGVDVNLVGGTGPKNRITQEDVQRYVKGVMTGQAAAPGKAAAAAAPAGGGELNLLPWPKVD
FTKFGPVEPKPLSRIKKISGANLHRNWVMIPHVTNNDEADITELEAFRVQMNKEHEKAGVKFTMLAFVIKAVVGALKKFP
TFNASLDGDNLVFKQYYHIGFAADTPNGLVVPVIRDADKKGVVDIAREMAELSKAAREGKLKPDQMQGGCFSISSLGGIG
GTHFTPIINAPEVAILGLSRGYQKPVWDGKQFVPRLTLPLSLSYDHRVIDGAEAARFNAYLAAVLADFRRVLL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=443, Percent_Identity=30.0225733634311, Blast_Score=163, Evalue=5e-40,
Organism=Homo sapiens, GI31711992, Length=425, Percent_Identity=31.5294117647059, Blast_Score=145, Evalue=8e-35,
Organism=Homo sapiens, GI19923748, Length=226, Percent_Identity=34.5132743362832, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI203098816, Length=461, Percent_Identity=26.8980477223427, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI203098753, Length=431, Percent_Identity=27.6102088167053, Blast_Score=119, Evalue=8e-27,
Organism=Homo sapiens, GI260898739, Length=163, Percent_Identity=35.5828220858896, Blast_Score=94, Evalue=4e-19,
Organism=Escherichia coli, GI1786305, Length=553, Percent_Identity=52.4412296564195, Blast_Score=500, Evalue=1e-143,
Organism=Escherichia coli, GI1786946, Length=438, Percent_Identity=30.8219178082192, Blast_Score=178, Evalue=8e-46,
Organism=Caenorhabditis elegans, GI17537937, Length=424, Percent_Identity=28.7735849056604, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17560088, Length=445, Percent_Identity=30.1123595505618, Blast_Score=148, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI25146366, Length=204, Percent_Identity=37.7450980392157, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17538894, Length=308, Percent_Identity=31.4935064935065, Blast_Score=115, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6320352, Length=417, Percent_Identity=30.4556354916067, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6324258, Length=436, Percent_Identity=28.4403669724771, Blast_Score=124, Evalue=3e-29,
Organism=Drosophila melanogaster, GI18859875, Length=435, Percent_Identity=31.0344827586207, Blast_Score=172, Evalue=7e-43,
Organism=Drosophila melanogaster, GI24645909, Length=207, Percent_Identity=35.2657004830918, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI20129315, Length=225, Percent_Identity=32.4444444444444, Blast_Score=125, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24582497, Length=225, Percent_Identity=32.4444444444444, Blast_Score=125, Evalue=9e-29,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 57225; Mature: 57094

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQVVEIKVPDIGDYKDVPVIELHVKAGDTVSAEDSLVTLESDKATMDVPSPKSGVVKEV
CCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCEEEEECCCCEEECCCCCCCCEEEE
KIKVGDAVSEGSLVLLLEEQGATAASAPQAAPAPAPVAAALAPAAQAPVSAPAAQPAAGG
EEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHCCHHCCCCCCCCCCCCCCC
GTIEVKVPDIGDYKDVPVIEISVKVGDKVEAEQSLITLESDKATMDVPSPAAGTVKDIRV
CEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEECCCCEECCCCCCCCCCEEEEE
KVGDAVSEGTLIVVLEGAGGAAAAAPAPAPAPAAAAPSPAPAVAPVAAPAAAPATYTADT
EECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
VGTIGKAAHASPSVRKYARELGVDVNLVGGTGPKNRITQEDVQRYVKGVMTGQAAAPGKA
HHHHCHHHCCCHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCH
AAAAAPAGGGELNLLPWPKVDFTKFGPVEPKPLSRIKKISGANLHRNWVMIPHVTNNDEA
HHHCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCEECCEEEEEEECCCCCC
DITELEAFRVQMNKEHEKAGVKFTMLAFVIKAVVGALKKFPTFNASLDGDNLVFKQYYHI
CHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEE
GFAADTPNGLVVPVIRDADKKGVVDIAREMAELSKAAREGKLKPDQMQGGCFSISSLGGI
EEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEECCCCCC
GGTHFTPIINAPEVAILGLSRGYQKPVWDGKQFVPRLTLPLSLSYDHRVIDGAEAARFNA
CCCCCCEECCCCCEEEEECCCCCCCCCCCCCHHCCEEEEEEEECCCCEEECCCHHHHHHH
YLAAVLADFRRVLL
HHHHHHHHHHHHHC
>Mature Secondary Structure 
SQVVEIKVPDIGDYKDVPVIELHVKAGDTVSAEDSLVTLESDKATMDVPSPKSGVVKEV
CCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCEEEEECCCCEEECCCCCCCCEEEE
KIKVGDAVSEGSLVLLLEEQGATAASAPQAAPAPAPVAAALAPAAQAPVSAPAAQPAAGG
EEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHCCHHCCCCCCCCCCCCCCC
GTIEVKVPDIGDYKDVPVIEISVKVGDKVEAEQSLITLESDKATMDVPSPAAGTVKDIRV
CEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEECCCCEECCCCCCCCCCEEEEE
KVGDAVSEGTLIVVLEGAGGAAAAAPAPAPAPAAAAPSPAPAVAPVAAPAAAPATYTADT
EECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
VGTIGKAAHASPSVRKYARELGVDVNLVGGTGPKNRITQEDVQRYVKGVMTGQAAAPGKA
HHHHCHHHCCCHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCH
AAAAAPAGGGELNLLPWPKVDFTKFGPVEPKPLSRIKKISGANLHRNWVMIPHVTNNDEA
HHHCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCEECCEEEEEEECCCCCC
DITELEAFRVQMNKEHEKAGVKFTMLAFVIKAVVGALKKFPTFNASLDGDNLVFKQYYHI
CHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEE
GFAADTPNGLVVPVIRDADKKGVVDIAREMAELSKAAREGKLKPDQMQGGCFSISSLGGI
EEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEECCCCCC
GGTHFTPIINAPEVAILGLSRGYQKPVWDGKQFVPRLTLPLSLSYDHRVIDGAEAARFNA
CCCCCCEECCCCCEEEEECCCCCCCCCCCCCHHCCEEEEEEEECCCCEEECCCHHHHHHH
YLAAVLADFRRVLL
HHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8021225 [H]