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The map label for this gene is phoB [H]

Identifier: 17545792

GI number: 17545792

Start: 1127395

End: 1128108

Strand: Direct

Name: phoB [H]

Synonym: RSc1073

Alternate gene names: 17545792

Gene position: 1127395-1128108 (Clockwise)

Preceding gene: 17545791

Following gene: 17545793

Centisome position: 30.34

GC content: 64.71

Gene sequence:

>714_bases
ATGGCGATACATATTGCCTCGGTAGAAGACAACCAGGCACAGTCGGAGCTGATTCGAGAGATCCTGGTCTCGTCCGGTTA
CGAATGCGCATCTTTCTCGAGCGGCGACGCCTTCGTGAAGGCGTTGCGCGACCGCACGTTCGATCTGCTGCTGCTCGACT
GGCAACTGCCGGACATCAGCGGCATCCAGCTGGTGAGCTGGGTGCGCCAGACGGTGGGCCCGGCGCTGCCGGTGCTGTTC
CTGACCAATCGCTCGCTCGAGGATGACGTCGTGCGCGGGTTGGCGGCCGGGGCGGATGACTACGTGGTCAAGCCGGTGCG
GCGCGCCGAACTGGTCGCGCGGGTCGGTGCGCTGTTGCGCCGCGCGGCGCCGCGTACCGGCGAATCGCTCGAGCGCATGC
GGGTGGGGCCTTATGCGGTGGATCCGCTGGGGCGCGTGCTGACGCTGCATGGCTTGCAGGTCGAGCTGTCGCCGCGCGAA
TTCGATCTGGCGCTCTATCTGTTCCGCAACGTGGGCAAGTTGGTGCCGCGCGAACTGATCGAGCAGGCGGTCTGGGGCCG
CAGCATCGGTCCGGACTCGCGCACGCTGGCTACGCATATCTCCAAGCTCCGGCTCAAGCTCGACCTCAACCAGAAGAATG
GTGTCCGGCTGGTGTCGGTGTATTCCCACGGTTATCGTCTCGAAACCACCCAGGAAAATGCCGAAGCACACTGA

Upstream 100 bases:

>100_bases
ATGATGAAGACACAGGGAGCTGGCCGGCGCCCCATGCAGCGGTGACGGCCCTTGTCGGCACGTCGCTGCCGGACCCGGAT
CGCGGGGAAGGGCAACCCAG

Downstream 100 bases:

>100_bases
GCCGCGGCCGCGCGGCTGGTTCGGTCTGGTCGCCTCGATCGGTGCGGCGCTACTGTGCTGTGCGATGTGGCGGCCGGCCA
CGGCGCAGCCGTCCGGCGCG

Product: two-component system response regulator transcription regulator protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MAIHIASVEDNQAQSELIREILVSSGYECASFSSGDAFVKALRDRTFDLLLLDWQLPDISGIQLVSWVRQTVGPALPVLF
LTNRSLEDDVVRGLAAGADDYVVKPVRRAELVARVGALLRRAAPRTGESLERMRVGPYAVDPLGRVLTLHGLQVELSPRE
FDLALYLFRNVGKLVPRELIEQAVWGRSIGPDSRTLATHISKLRLKLDLNQKNGVRLVSVYSHGYRLETTQENAEAH

Sequences:

>Translated_237_residues
MAIHIASVEDNQAQSELIREILVSSGYECASFSSGDAFVKALRDRTFDLLLLDWQLPDISGIQLVSWVRQTVGPALPVLF
LTNRSLEDDVVRGLAAGADDYVVKPVRRAELVARVGALLRRAAPRTGESLERMRVGPYAVDPLGRVLTLHGLQVELSPRE
FDLALYLFRNVGKLVPRELIEQAVWGRSIGPDSRTLATHISKLRLKLDLNQKNGVRLVSVYSHGYRLETTQENAEAH
>Mature_236_residues
AIHIASVEDNQAQSELIREILVSSGYECASFSSGDAFVKALRDRTFDLLLLDWQLPDISGIQLVSWVRQTVGPALPVLFL
TNRSLEDDVVRGLAAGADDYVVKPVRRAELVARVGALLRRAAPRTGESLERMRVGPYAVDPLGRVLTLHGLQVELSPREF
DLALYLFRNVGKLVPRELIEQAVWGRSIGPDSRTLATHISKLRLKLDLNQKNGVRLVSVYSHGYRLETTQENAEAH

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=231, Percent_Identity=34.6320346320346, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI1786784, Length=221, Percent_Identity=33.4841628959276, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI87082012, Length=207, Percent_Identity=32.8502415458937, Blast_Score=104, Evalue=4e-24,
Organism=Escherichia coli, GI1790860, Length=202, Percent_Identity=36.1386138613861, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1789402, Length=205, Percent_Identity=33.6585365853659, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1790552, Length=210, Percent_Identity=34.2857142857143, Blast_Score=100, Evalue=6e-23,
Organism=Escherichia coli, GI1787229, Length=236, Percent_Identity=31.3559322033898, Blast_Score=96, Evalue=1e-21,
Organism=Escherichia coli, GI1788394, Length=224, Percent_Identity=31.6964285714286, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI1786911, Length=224, Percent_Identity=34.8214285714286, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790863, Length=232, Percent_Identity=28.8793103448276, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI2367329, Length=215, Percent_Identity=34.8837209302326, Blast_Score=86, Evalue=1e-18,
Organism=Escherichia coli, GI1787375, Length=222, Percent_Identity=28.8288288288288, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1789809, Length=227, Percent_Identity=25.5506607929515, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI145693140, Length=230, Percent_Identity=27.8260869565217, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1790437, Length=104, Percent_Identity=31.7307692307692, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1790299, Length=108, Percent_Identity=33.3333333333333, Blast_Score=60, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26363; Mature: 26231

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIHIASVEDNQAQSELIREILVSSGYECASFSSGDAFVKALRDRTFDLLLLDWQLPDIS
CEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCEEEEEEEECCCCCC
GIQLVSWVRQTVGPALPVLFLTNRSLEDDVVRGLAAGADDYVVKPVRRAELVARVGALLR
HHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
RAAPRTGESLERMRVGPYAVDPLGRVLTLHGLQVELSPREFDLALYLFRNVGKLVPRELI
HCCCCCCHHHHHHCCCCCCCCCHHHHHEEECEEEEECCCHHHHHHHHHHHHHHHHHHHHH
EQAVWGRSIGPDSRTLATHISKLRLKLDLNQKNGVRLVSVYSHGYRLETTQENAEAH
HHHHCCCCCCCCCHHHHHHHHHHEEEEECCCCCCEEEEEEECCCEEEEECCCCCCCC
>Mature Secondary Structure 
AIHIASVEDNQAQSELIREILVSSGYECASFSSGDAFVKALRDRTFDLLLLDWQLPDIS
EEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCEEEEEEEECCCCCC
GIQLVSWVRQTVGPALPVLFLTNRSLEDDVVRGLAAGADDYVVKPVRRAELVARVGALLR
HHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
RAAPRTGESLERMRVGPYAVDPLGRVLTLHGLQVELSPREFDLALYLFRNVGKLVPRELI
HCCCCCCHHHHHHCCCCCCCCCHHHHHEEECEEEEECCCHHHHHHHHHHHHHHHHHHHHH
EQAVWGRSIGPDSRTLATHISKLRLKLDLNQKNGVRLVSVYSHGYRLETTQENAEAH
HHHHCCCCCCCCCHHHHHHHHHHEEEEECCCCCCEEEEEEECCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]