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The map label for this gene is pmbA [H]

Identifier: 17545664

GI number: 17545664

Start: 992921

End: 994294

Strand: Direct

Name: pmbA [H]

Synonym: RSc0945

Alternate gene names: 17545664

Gene position: 992921-994294 (Clockwise)

Preceding gene: 17545661

Following gene: 17545667

Centisome position: 26.72

GC content: 67.69

Gene sequence:

>1374_bases
ATGTCCGACACGCTCGATTCCAAGACCGATTCCGTTTTTGCCTACCGCCGCGAGCGGCTCGAGGAAATGGCTCGCGATGT
GCTGCGCATTGCACGCGAACTCGGCGCCACCGATGCCGCCACGGAAATCTCCGAAGGCCAAGGCTTGTCGGTCACGGTGC
GCAAGGGCGAGATCGAGACCATCGAGCAGAACCGCGACAAGGTGGTCGGCGTGACGGTGATGCTGGGCAAGAAGCGCGGC
AACGCCAGCACCTCGGATTTTTCGCCGGCCGCGCTGCGCTCCACCGTCGAGGCGGCCTACAACATCGCGCGCTTCACCGC
CGATGACGACTGCGCCGGGCTGGCCGAAGAGGAATTGCTCGAGCGCCAACCGCGCGACCTGTCGCTGTACCACCCGTGGA
CGCTCGACGCCGAAGCCGCCGTGGAGATCGCCCGTGCCGCCGAGGCCGCCGCCTTCGCGGTGAGCCCGAAGATCCGCAAC
AGCGACGGCGCCAGCGTGTCGGCGCAGCATTCGCATTTCGTCCTGGCGACCTCGCGCGGGTTCCTGGGCGGCTATCCGTA
TTCGCGGCATTTCATTTCGTGCGCGCCGATCGCCGGCTCGGGCCAGCAGATGCAGCGCGACGACTGGTATTCGTCCAAGC
GCTCGCCCAAGGACCTGGCGGCACCCGAGGCCATCGGCCAGTACGCTGCCGAGCGCGCGCTGGCGCGGCTCGCCGCGCGC
CGGCTGAGCACCCGCAAGTGTCCGGTGCTGTTCGAGGCGCCGCTGGCGGCGGGCCTGCTGGGCGCGTTCGTCCAGGCTGT
GTCGGGCGGTGCGCTGTATCGCAAGTCGACCTTCCTGCTCGACTCGCTCGGCAAGGCAATCTTCGCGCCGCACGTGCAGA
TCAAGGAAGACCCGCACGTGCCCAGCGGGATGGGCAGCGCGCCGTTCGACGAGGAAGGCGTGCGCACGCAGCGCCGTGAC
GTGGTGAGGGATGGCGTCGTCGAAGGCTATTTCCTGTCGACGTATTCCGCGCGCAAGCTGGGCATGCAGACCACCGGCAA
CGCGGGCGGCTCGCACAACCTGACGCTGTTCTCCGACCGGACGCAGCCGGGCGACGATTTCGCCGGCATGCTGCGCCGCA
TGGGCACGGGCCTGCTGGTGACGGAGCTGATGGGGCAGGGCGTCAACTATGTGACCGGCGACTATTCGCGCGGCGCGTCG
GGCTACTGGGTGGAGAACGGCGTGATCCAGTACCCGGTCGAGGAGATCACCATTGCCGGCAACCTGCGCGACATGTTCCG
CCAGATCGTCGCCATCGGGGCGGATGCGCTGATCCGGGGCACCAAGGAAACCGGTTCGATCCTGATCGAGCAGATGACGA
TCGCCGGCGAGTAA

Upstream 100 bases:

>100_bases
GCTCATGGCGCGCATTGTATCTTGCTATGATGGCCGCTTAAGCTAAAAGCGCGCCGAAAACGCACCAAGCGGCGCCCCAA
CGATTTCCTCTCTCCTGCCT

Downstream 100 bases:

>100_bases
GCCGCGTCGCCCCAACGAAAAACGCGCTCACCGATGAGCGCGTTTTTTCATGCCTGTCTCATGCCGGTTGCCGCCGGCTT
CATTGCTCGTCCGACGGCGG

Product: PMBA protein (tlde protein)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MSDTLDSKTDSVFAYRRERLEEMARDVLRIARELGATDAATEISEGQGLSVTVRKGEIETIEQNRDKVVGVTVMLGKKRG
NASTSDFSPAALRSTVEAAYNIARFTADDDCAGLAEEELLERQPRDLSLYHPWTLDAEAAVEIARAAEAAAFAVSPKIRN
SDGASVSAQHSHFVLATSRGFLGGYPYSRHFISCAPIAGSGQQMQRDDWYSSKRSPKDLAAPEAIGQYAAERALARLAAR
RLSTRKCPVLFEAPLAAGLLGAFVQAVSGGALYRKSTFLLDSLGKAIFAPHVQIKEDPHVPSGMGSAPFDEEGVRTQRRD
VVRDGVVEGYFLSTYSARKLGMQTTGNAGGSHNLTLFSDRTQPGDDFAGMLRRMGTGLLVTELMGQGVNYVTGDYSRGAS
GYWVENGVIQYPVEEITIAGNLRDMFRQIVAIGADALIRGTKETGSILIEQMTIAGE

Sequences:

>Translated_457_residues
MSDTLDSKTDSVFAYRRERLEEMARDVLRIARELGATDAATEISEGQGLSVTVRKGEIETIEQNRDKVVGVTVMLGKKRG
NASTSDFSPAALRSTVEAAYNIARFTADDDCAGLAEEELLERQPRDLSLYHPWTLDAEAAVEIARAAEAAAFAVSPKIRN
SDGASVSAQHSHFVLATSRGFLGGYPYSRHFISCAPIAGSGQQMQRDDWYSSKRSPKDLAAPEAIGQYAAERALARLAAR
RLSTRKCPVLFEAPLAAGLLGAFVQAVSGGALYRKSTFLLDSLGKAIFAPHVQIKEDPHVPSGMGSAPFDEEGVRTQRRD
VVRDGVVEGYFLSTYSARKLGMQTTGNAGGSHNLTLFSDRTQPGDDFAGMLRRMGTGLLVTELMGQGVNYVTGDYSRGAS
GYWVENGVIQYPVEEITIAGNLRDMFRQIVAIGADALIRGTKETGSILIEQMTIAGE
>Mature_456_residues
SDTLDSKTDSVFAYRRERLEEMARDVLRIARELGATDAATEISEGQGLSVTVRKGEIETIEQNRDKVVGVTVMLGKKRGN
ASTSDFSPAALRSTVEAAYNIARFTADDDCAGLAEEELLERQPRDLSLYHPWTLDAEAAVEIARAAEAAAFAVSPKIRNS
DGASVSAQHSHFVLATSRGFLGGYPYSRHFISCAPIAGSGQQMQRDDWYSSKRSPKDLAAPEAIGQYAAERALARLAARR
LSTRKCPVLFEAPLAAGLLGAFVQAVSGGALYRKSTFLLDSLGKAIFAPHVQIKEDPHVPSGMGSAPFDEEGVRTQRRDV
VRDGVVEGYFLSTYSARKLGMQTTGNAGGSHNLTLFSDRTQPGDDFAGMLRRMGTGLLVTELMGQGVNYVTGDYSRGASG
YWVENGVIQYPVEEITIAGNLRDMFRQIVAIGADALIRGTKETGSILIEQMTIAGE

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=454, Percent_Identity=47.3568281938326, Blast_Score=418, Evalue=1e-118,
Organism=Escherichia coli, GI1789640, Length=397, Percent_Identity=22.4181360201511, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 49361; Mature: 49230

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTLDSKTDSVFAYRRERLEEMARDVLRIARELGATDAATEISEGQGLSVTVRKGEIET
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEEEECCCHHH
IEQNRDKVVGVTVMLGKKRGNASTSDFSPAALRSTVEAAYNIARFTADDDCAGLAEEELL
HHCCCCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
ERQPRDLSLYHPWTLDAEAAVEIARAAEAAAFAVSPKIRNSDGASVSAQHSHFVLATSRG
HCCCCCEEEECCCCCCHHHHHHHHHHHHHHHEEECCCCCCCCCCCEECCCCEEEEEECCC
FLGGYPYSRHFISCAPIAGSGQQMQRDDWYSSKRSPKDLAAPEAIGQYAAERALARLAAR
CCCCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCHHCCHHHHHHHHHHHHHHHHHHH
RLSTRKCPVLFEAPLAAGLLGAFVQAVSGGALYRKSTFLLDSLGKAIFAPHVQIKEDPHV
HHCCCCCCEEEECHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCC
PSGMGSAPFDEEGVRTQRRDVVRDGVVEGYFLSTYSARKLGMQTTGNAGGSHNLTLFSDR
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECHHHHHCCCCCCCCCCCCCCEEEEECC
TQPGDDFAGMLRRMGTGLLVTELMGQGVNYVTGDYSRGASGYWVENGVIQYPVEEITIAG
CCCCHHHHHHHHHHCCCHHHHHHHHCCCCEEECCCCCCCCCEEEECCEEECCHHHEEEEC
NLRDMFRQIVAIGADALIRGTKETGSILIEQMTIAGE
CHHHHHHHHHHHCCHHHHCCCCHHHHEEEEHHHHCCC
>Mature Secondary Structure 
SDTLDSKTDSVFAYRRERLEEMARDVLRIARELGATDAATEISEGQGLSVTVRKGEIET
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEEEECCCHHH
IEQNRDKVVGVTVMLGKKRGNASTSDFSPAALRSTVEAAYNIARFTADDDCAGLAEEELL
HHCCCCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
ERQPRDLSLYHPWTLDAEAAVEIARAAEAAAFAVSPKIRNSDGASVSAQHSHFVLATSRG
HCCCCCEEEECCCCCCHHHHHHHHHHHHHHHEEECCCCCCCCCCCEECCCCEEEEEECCC
FLGGYPYSRHFISCAPIAGSGQQMQRDDWYSSKRSPKDLAAPEAIGQYAAERALARLAAR
CCCCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCHHCCHHHHHHHHHHHHHHHHHHH
RLSTRKCPVLFEAPLAAGLLGAFVQAVSGGALYRKSTFLLDSLGKAIFAPHVQIKEDPHV
HHCCCCCCEEEECHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCC
PSGMGSAPFDEEGVRTQRRDVVRDGVVEGYFLSTYSARKLGMQTTGNAGGSHNLTLFSDR
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECHHHHHCCCCCCCCCCCCCCEEEEECC
TQPGDDFAGMLRRMGTGLLVTELMGQGVNYVTGDYSRGASGYWVENGVIQYPVEEITIAG
CCCCHHHHHHHHHHCCCHHHHHHHHCCCCEEECCCCCCCCCEEEECCEEECCHHHEEEEC
NLRDMFRQIVAIGADALIRGTKETGSILIEQMTIAGE
CHHHHHHHHHHHCCHHHHCCCCHHHHEEEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]