Click here to switch to the map view.

The map label for this gene is trmD

Identifier: 17545654

GI number: 17545654

Start: 985211

End: 985999

Strand: Direct

Name: trmD

Synonym: RSc0935

Alternate gene names: 17545654

Gene position: 985211-985999 (Clockwise)

Preceding gene: 17545653

Following gene: 17545655

Centisome position: 26.51

GC content: 67.17

Gene sequence:

>789_bases
ATGCAGTTCGATGTCATCTCGCTGTTCCCGGAGATGTTCCGGGCGTTGACCGACTGGGGGATCACCAGCCGGGCAGCCAA
GCAGCAGGTGTACACGTTGCGTACCTGGAACCCGCGCGACTTCACGACGGACAATTACCGTACCGTGGATGACCGGCCCT
ATGGCGGCGGCCCAGGCATGGTGATGCTGGCCAAGCCGCTGGAAGCGGCGCTCGATGCGATCCGCGAGGCGCAGGCGCCC
GCGCCGTCGCATGTGGTGCTGCTGTCGCCGCAAGGCCGGCCGCTGACCCACCGGCGGGTGATGGAGCTGGCGCAGCTGCC
GGCGCTGACGCTGCTGTGCGGCCGCTACGAGGCCATCGACCAGCGTCTGGTCGACCGGCGCGTCGATGAAGAAATTAGCC
TCGGCGATTTCGTGCTGTCCGGCGGTGAGATCGCCGCCATGGCTATCATCGACGCCGTGGTGCGGCAGTTGCCCGGGGTG
CTGGGCGATGCGCAGTCGGCGGTGCAGGACAGTTTCGTCAACGGCCTGCTGGATTGCCCGCATTACACGCGGCCGGAAGA
ATACGAAGGCGTGCGTGTGCCCGATGTGCTGCTGGGCGGCCACCACGCCGAGATCGAGAAGTGGCGCCGCCAGCAGGCGC
TGTTGAATACGATGCGCAAGCGGCCCGATCTGATCGAGGCGGCGCGCAGGCAGGGTTTGTTGTCCCGCAGCGATGAGGTC
TTCCTCGCTGCCGCGGCGACGACGGCAAAGGGGTCGGGCGCTTCCCTATCGGAAGCCCCGGCCAAGTGA

Upstream 100 bases:

>100_bases
CGTTCGTCGAGGTGTATGTGAAGTCGGTGGATGTGGCGGGCAAGCACATCGTCGTCGACTGGGGCCTGGATTACTGAACA
TGCGGCCGGAGAATTCGGCA

Downstream 100 bases:

>100_bases
TGTTCCCATCCTCTACCGGGGCCGCGGTCGGTTGACCGGGCAGAACGCCGGCATGATGGTTCAGGAGAAAAAGATGAATC
TCATCGAGCAAATCGAGCAG

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MQFDVISLFPEMFRALTDWGITSRAAKQQVYTLRTWNPRDFTTDNYRTVDDRPYGGGPGMVMLAKPLEAALDAIREAQAP
APSHVVLLSPQGRPLTHRRVMELAQLPALTLLCGRYEAIDQRLVDRRVDEEISLGDFVLSGGEIAAMAIIDAVVRQLPGV
LGDAQSAVQDSFVNGLLDCPHYTRPEEYEGVRVPDVLLGGHHAEIEKWRRQQALLNTMRKRPDLIEAARRQGLLSRSDEV
FLAAAATTAKGSGASLSEAPAK

Sequences:

>Translated_262_residues
MQFDVISLFPEMFRALTDWGITSRAAKQQVYTLRTWNPRDFTTDNYRTVDDRPYGGGPGMVMLAKPLEAALDAIREAQAP
APSHVVLLSPQGRPLTHRRVMELAQLPALTLLCGRYEAIDQRLVDRRVDEEISLGDFVLSGGEIAAMAIIDAVVRQLPGV
LGDAQSAVQDSFVNGLLDCPHYTRPEEYEGVRVPDVLLGGHHAEIEKWRRQQALLNTMRKRPDLIEAARRQGLLSRSDEV
FLAAAATTAKGSGASLSEAPAK
>Mature_262_residues
MQFDVISLFPEMFRALTDWGITSRAAKQQVYTLRTWNPRDFTTDNYRTVDDRPYGGGPGMVMLAKPLEAALDAIREAQAP
APSHVVLLSPQGRPLTHRRVMELAQLPALTLLCGRYEAIDQRLVDRRVDEEISLGDFVLSGGEIAAMAIIDAVVRQLPGV
LGDAQSAVQDSFVNGLLDCPHYTRPEEYEGVRVPDVLLGGHHAEIEKWRRQQALLNTMRKRPDLIEAARRQGLLSRSDEV
FLAAAATTAKGSGASLSEAPAK

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=226, Percent_Identity=56.6371681415929, Blast_Score=261, Evalue=3e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_RALSO (Q8Y0V8)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_519056.1
- ProteinModelPortal:   Q8Y0V8
- SMR:   Q8Y0V8
- GeneID:   1219743
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc0935
- NMPDR:   fig|267608.1.peg.935
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   PRK00026
- BioCyc:   RSOL267608:RSC0935-MONOMER
- BRENDA:   2.1.1.31
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 28871; Mature: 28871

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: BINDING 114-114

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFDVISLFPEMFRALTDWGITSRAAKQQVYTLRTWNPRDFTTDNYRTVDDRPYGGGPGM
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHEEEEECCCCCCCCCCCCEECCCCCCCCCCCE
VMLAKPLEAALDAIREAQAPAPSHVVLLSPQGRPLTHRRVMELAQLPALTLLCGRYEAID
EEEHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QRLVDRRVDEEISLGDFVLSGGEIAAMAIIDAVVRQLPGVLGDAQSAVQDSFVNGLLDCP
HHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCHHCHHHHHHHHHHHHHHHCCC
HYTRPEEYEGVRVPDVLLGGHHAEIEKWRRQQALLNTMRKRPDLIEAARRQGLLSRSDEV
CCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHCCCCCE
FLAAAATTAKGSGASLSEAPAK
EEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQFDVISLFPEMFRALTDWGITSRAAKQQVYTLRTWNPRDFTTDNYRTVDDRPYGGGPGM
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHEEEEECCCCCCCCCCCCEECCCCCCCCCCCE
VMLAKPLEAALDAIREAQAPAPSHVVLLSPQGRPLTHRRVMELAQLPALTLLCGRYEAID
EEEHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QRLVDRRVDEEISLGDFVLSGGEIAAMAIIDAVVRQLPGVLGDAQSAVQDSFVNGLLDCP
HHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCHHCHHHHHHHHHHHHHHHCCC
HYTRPEEYEGVRVPDVLLGGHHAEIEKWRRQQALLNTMRKRPDLIEAARRQGLLSRSDEV
CCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHCCCCCE
FLAAAATTAKGSGASLSEAPAK
EEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852