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The map label for this gene is gltJ [H]

Identifier: 17545201

GI number: 17545201

Start: 518429

End: 519157

Strand: Direct

Name: gltJ [H]

Synonym: RSc0482

Alternate gene names: 17545201

Gene position: 518429-519157 (Clockwise)

Preceding gene: 17545200

Following gene: 17545202

Centisome position: 13.95

GC content: 63.1

Gene sequence:

>729_bases
ATGAATTACCACTGGAACTGGGGCGTCTTCTTCACCGAAGCCGCGCAGAATCAGACCTACCTTGACTGGCTGCTTTCGGG
GTTGAAGACCACGATCGCGCTGGGCCTGTCCTCCTGGATCATCGCGCTCGTCCTTGGCTCCGTGCTGGGCGTGCTGCGCA
CCGTGCCCAGCAAGGCGCTGTCGGGCCTTGCCACGGCCTACGTGGAGCTGTTCCGCAACATCCCGCTGATCGTGCAGCTG
TTCATCTGGTATTTCGTCATGCCGGAACTGGTGCCGGGCGGCGACTACATCAAGCAGATGAACCCCGCCACGGCGATGTT
CCTGTGCGCGATGCTGTGCCTCGGTACTTTTACCGCGGCGCGGGTGTGCGAACAGGTGCGCTCGGGCATCAACTCGCTGC
CGCGCGGCCAGCGCAACGCCGGCCTGGCGATGGGCTTCACGCTGGCGCAGACGTACCGCCACGTCATGCTGCCGATGGCG
TTCCGCATCATCGTGCCGCCGCTCACCTCCGAGTTCCTGAACATCTTCAAGAACTCCGCGGTGGCGTCGACCATCGGTCT
GCTGGAGCTCGCGGCACAGGGGCGCCAACTGGTGGACTACACCGCGCAGCCCTACGAGTCGTTCATCGCCGTGACACTGA
TGTACATGCTGATCAACCTGACCGTCATGGGAATCATGCGCTGGGTTGAAGCACGTACCCGGCTGCCCGGTTTCATCGGC
GGCAAGTAA

Upstream 100 bases:

>100_bases
ATCGGCTCCCTCAGGAACGAGCAACGCTGAGCATCATTGAAACGGAAGGCAAAGGCCGGTTGACAGGCCCTCCCTTCCGT
TTCTTTTTGAGGGAACCATC

Downstream 100 bases:

>100_bases
GCCGGCACGCGAGGATATTCATGGCTTATCAATTCGATTTCACGTCCGTCAACGCCGACAACCTGCGCGTGCTCGGCGAA
GGCATGGTCCTGTCGCTCGA

Product: glutamate/aspartate transmembrane ABC transporter protein

Products: ADP; phosphate; L-glutamate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MNYHWNWGVFFTEAAQNQTYLDWLLSGLKTTIALGLSSWIIALVLGSVLGVLRTVPSKALSGLATAYVELFRNIPLIVQL
FIWYFVMPELVPGGDYIKQMNPATAMFLCAMLCLGTFTAARVCEQVRSGINSLPRGQRNAGLAMGFTLAQTYRHVMLPMA
FRIIVPPLTSEFLNIFKNSAVASTIGLLELAAQGRQLVDYTAQPYESFIAVTLMYMLINLTVMGIMRWVEARTRLPGFIG
GK

Sequences:

>Translated_242_residues
MNYHWNWGVFFTEAAQNQTYLDWLLSGLKTTIALGLSSWIIALVLGSVLGVLRTVPSKALSGLATAYVELFRNIPLIVQL
FIWYFVMPELVPGGDYIKQMNPATAMFLCAMLCLGTFTAARVCEQVRSGINSLPRGQRNAGLAMGFTLAQTYRHVMLPMA
FRIIVPPLTSEFLNIFKNSAVASTIGLLELAAQGRQLVDYTAQPYESFIAVTLMYMLINLTVMGIMRWVEARTRLPGFIG
GK
>Mature_242_residues
MNYHWNWGVFFTEAAQNQTYLDWLLSGLKTTIALGLSSWIIALVLGSVLGVLRTVPSKALSGLATAYVELFRNIPLIVQL
FIWYFVMPELVPGGDYIKQMNPATAMFLCAMLCLGTFTAARVCEQVRSGINSLPRGQRNAGLAMGFTLAQTYRHVMLPMA
FRIIVPPLTSEFLNIFKNSAVASTIGLLELAAQGRQLVDYTAQPYESFIAVTLMYMLINLTVMGIMRWVEARTRLPGFIG
GK

Specific function: Part of the binding-protein-dependent transport system for aspartate/glutamate; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0765

COG function: function code E; ABC-type amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786874, Length=246, Percent_Identity=58.5365853658537, Blast_Score=298, Evalue=2e-82,
Organism=Escherichia coli, GI1788226, Length=195, Percent_Identity=32.3076923076923, Blast_Score=104, Evalue=4e-24,
Organism=Escherichia coli, GI1786873, Length=214, Percent_Identity=27.5700934579439, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787030, Length=215, Percent_Identity=30.6976744186047, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787086, Length=213, Percent_Identity=27.6995305164319, Blast_Score=84, Evalue=6e-18,
Organism=Escherichia coli, GI48994930, Length=122, Percent_Identity=35.2459016393443, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788645, Length=210, Percent_Identity=28.0952380952381, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787087, Length=223, Percent_Identity=23.7668161434978, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87082239, Length=194, Percent_Identity=25.7731958762887, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788646, Length=211, Percent_Identity=25.1184834123223, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 26871; Mature: 26871

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CC
>Mature Secondary Structure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CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamate [Periplasm] + H2O = ADP + phosphate + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]