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The map label for this gene is topA [H]

Identifier: 17230272

GI number: 17230272

Start: 3376722

End: 3379367

Strand: Direct

Name: topA [H]

Synonym: alr2780

Alternate gene names: 17230272

Gene position: 3376722-3379367 (Clockwise)

Preceding gene: 17230267

Following gene: 17230273

Centisome position: 52.65

GC content: 48.26

Gene sequence:

>2646_bases
ATGTCAACTCTCGTCATTGTCGAATCTCCAACCAAAGCTCGTACCATTCGTAACTACCTGCCAAAAGACTATCGGGTAGA
AGCGTCGATGGGTCATGTGCGTGACCTACCCCAGTCAGCTAGTGAAATTCCCGCCAGTGTTAAAGGGGAAGCATGGGCGC
AGCTTGGGGTAAATGTGGACGCGGACTTTGAACCAGTGTATGTTGTCCCCAAAGACAAAAAGAAAGTTGTCACCCAGCTC
AAAGAAGCGCTGAAAGATGCTGACGAACTGATTCTAGCAACTGACGAAGACCGAGAAGGTGAAAGCATCAGTTGGCATTT
ATTCCAGTTGCTGAAGCCAAAAGTACCGACAAAGCGGATGGTGTTTCATGAGATTACCCAAGAAGCCATCAAAAAAGCTT
TGAAGGACTGCCGCAACATCGACGAGCAGCTAGTCCGCGCCCAGGAAACACGGCGGATTTTGGATCGTTTGGTGGGCTAT
ACCCTCTCCCCTCTGCTGTGGAAAAAAATTGCTTGGGGACTATCTGCGGGACGGGTACAGTCTGTGGCGGTGCGACTGTT
GGTAACCAAAGAACGCCAACGCCGGGCTTTCCATGAAGGTACCTACTGGGATTTAAAGGCGTATTTGGAGCAAAGCAAAA
GTCCATTTACCGCCCAGCTAACCACCCTGGCAGGCACGAAAGTGGCAACTGGGAGCGATTTTGATGCGTCTACCGGCAGA
ATTACCGCCGGGCGTAATGTGGTGCTGCTGAGTGAAACAGAAGCAGTTGCTCTCCAAGAACGGCTGACTGGTAAACCTTG
GAATGTCTCAGAGGTAGAAGAACGCCCAGTGACGCGCAAACCCGCGCCACCCTTTACCACTTCGACATTACAGCAGGAAT
CTAACCGGAAACTGCGCCTGTCAGCAAGGGACACCATGCGGATTGCCCAGAATTTATACGAGCAAGGGTATATTACCTAT
ATGCGTACAGATTCAGTGCATTTGTCAGAACAGGCGATCGCAGCTGCTCGCAATTGTGTAGAACAATTATACGGCAAACA
ATATCTCAGTCCCCAGAAGCGCCAATACACCACCAAATCCAAAGGCGCACAAGAAGCCCACGAAGCCATTCGTCCGGCTG
GTAGCACCTTCCGCACGCCCCAAGAAACCGGTTTAGGTGGTCGAGAACTTGCCCTCTATGATTTGATTTGGAAGCGTACC
GTCGCCTGTCAAATGGCTGACTCTCGCCAAACCCAAATTACTGTGCAGTTGCAAGTAGAAGACGCTGGTTTCCGTGCTTC
TGGTAAGCGGATTGACTTCCCTGGATATTTACGCGCCTACGTTGAAGGTTCCGATGACCCAGAAGCAGCCTTGGAAGACC
AAGAGGTAATTTTGCCCAGTCTGAAAGTTGGGGATCATCCAGATTGTCAAAATATCGAAGCAGTTGGTCACGAAACCCAA
CCCCCAGCTAGATACACCGAAGCGACCCTAGTCAAAACCCTAGAAAGTGAAGGCATTGGTCGTCCTAGTACCTACGCCAG
CATTATCGGCACAATTATCGATAAGGGTTATTCCCAATTGGTGAATAACGCCCTCATTCCCACCTTTACCGCTTTCGCCG
TCACCGACTTGTTGGAAAAAAATTTCCCTGATATCGTTGACCCCAGCTTTACCTCCAAAATGGAGCAAACCTTGGATGAT
ATTGCTGACGGTGAAGTTGAGTGGCTACCCTACTTAAAGAAATTTTATCTAGGGGATAAAGGTTTGGAAACTCTGGTCAG
AGAACGAGAAAGCTTAATTGATGCCAGTAAAGCCAGAACTGTTGAACTAGAAAACTTAGACGCTAAAGTCCGTATTGGTA
AATATGGGCCTTACATTGAAGTGGAAAACGGTGACGGTGTTGTCACAGCCTCAATTCCTAAAGACTTAACACCAGCTGAC
CTCGACCCCAAACAGGTGGAAATAATCCTGCGGCAAAAAACCGTCGGGCCAGACCAACTAGGCCGCCATCCTGAAACCGG
CGAACCAATTTACGTGAAAATTGGTGCTTACGGGCCTTATGTCCAGTTGGGTGATAAGTCTGATGAAAATCCCAAACCCA
AACAAGCTTCTCTACCCAAGGGAGTTACCCCAGAAAACATCACCTTAGAGAAGGCTATTGGTTTGTTATCACTACCCCGA
ACCTTAGGAACCCATCCTGGTACTGGCGGGAAGATCCAAGCCAGTCTAGGACGTTTTGGCCCTTACGTCGTCCATGACCA
AGGTAAAGAAGGTAAAGACTACCGTTCTCTCAAAGCGAGTGATGATGTTTTAACAATTTCTTTAGAAAGAGCGCTGGAGT
TGATATCCGAACCGAAGAAGACCCGTGGTTCTACTAGTAGTAAATCAAAGGCTGCTTTACGGGAATTAGGTACACACCCA
GAGGATGATGCACCCATAAATATCTACGACGGCCCCTATGGCCCTTATATTAAACACGGTAAGACCAATGTAGGTCTTCC
TGAGGGGACTTCAATGGAAGATGTAACATTAGCTACGGCATTAGAGTTACTATCTGCCAAAACGGCAACGGCTAAAACCA
CACGCAAAACAAGTAAAACCAGTACAACTAAATCCAAATCAACGGCAAAATCATCTAACGCTGCTACGAAAAAGAAGGGG
GCGTAA

Upstream 100 bases:

>100_bases
GCTCAATCTAAAGCAATCGTGACGTTTCATCTTGACATTTCATCGAAGTTAACTCAATTTGACTGTTTAGCTTCACAGCT
ATTCTTTTTTTTGAAATTCC

Downstream 100 bases:

>100_bases
GGTTAATGGTCAACAGTCAACAGTCCAAGATTTTCGACTATGGACTAATGACCAATAACCAATGACCGCCCTTAGATGCA
GTCCGATAGGTTGCTAATGT

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 881; Mature: 880

Protein sequence:

>881_residues
MSTLVIVESPTKARTIRNYLPKDYRVEASMGHVRDLPQSASEIPASVKGEAWAQLGVNVDADFEPVYVVPKDKKKVVTQL
KEALKDADELILATDEDREGESISWHLFQLLKPKVPTKRMVFHEITQEAIKKALKDCRNIDEQLVRAQETRRILDRLVGY
TLSPLLWKKIAWGLSAGRVQSVAVRLLVTKERQRRAFHEGTYWDLKAYLEQSKSPFTAQLTTLAGTKVATGSDFDASTGR
ITAGRNVVLLSETEAVALQERLTGKPWNVSEVEERPVTRKPAPPFTTSTLQQESNRKLRLSARDTMRIAQNLYEQGYITY
MRTDSVHLSEQAIAAARNCVEQLYGKQYLSPQKRQYTTKSKGAQEAHEAIRPAGSTFRTPQETGLGGRELALYDLIWKRT
VACQMADSRQTQITVQLQVEDAGFRASGKRIDFPGYLRAYVEGSDDPEAALEDQEVILPSLKVGDHPDCQNIEAVGHETQ
PPARYTEATLVKTLESEGIGRPSTYASIIGTIIDKGYSQLVNNALIPTFTAFAVTDLLEKNFPDIVDPSFTSKMEQTLDD
IADGEVEWLPYLKKFYLGDKGLETLVRERESLIDASKARTVELENLDAKVRIGKYGPYIEVENGDGVVTASIPKDLTPAD
LDPKQVEIILRQKTVGPDQLGRHPETGEPIYVKIGAYGPYVQLGDKSDENPKPKQASLPKGVTPENITLEKAIGLLSLPR
TLGTHPGTGGKIQASLGRFGPYVVHDQGKEGKDYRSLKASDDVLTISLERALELISEPKKTRGSTSSKSKAALRELGTHP
EDDAPINIYDGPYGPYIKHGKTNVGLPEGTSMEDVTLATALELLSAKTATAKTTRKTSKTSTTKSKSTAKSSNAATKKKG
A

Sequences:

>Translated_881_residues
MSTLVIVESPTKARTIRNYLPKDYRVEASMGHVRDLPQSASEIPASVKGEAWAQLGVNVDADFEPVYVVPKDKKKVVTQL
KEALKDADELILATDEDREGESISWHLFQLLKPKVPTKRMVFHEITQEAIKKALKDCRNIDEQLVRAQETRRILDRLVGY
TLSPLLWKKIAWGLSAGRVQSVAVRLLVTKERQRRAFHEGTYWDLKAYLEQSKSPFTAQLTTLAGTKVATGSDFDASTGR
ITAGRNVVLLSETEAVALQERLTGKPWNVSEVEERPVTRKPAPPFTTSTLQQESNRKLRLSARDTMRIAQNLYEQGYITY
MRTDSVHLSEQAIAAARNCVEQLYGKQYLSPQKRQYTTKSKGAQEAHEAIRPAGSTFRTPQETGLGGRELALYDLIWKRT
VACQMADSRQTQITVQLQVEDAGFRASGKRIDFPGYLRAYVEGSDDPEAALEDQEVILPSLKVGDHPDCQNIEAVGHETQ
PPARYTEATLVKTLESEGIGRPSTYASIIGTIIDKGYSQLVNNALIPTFTAFAVTDLLEKNFPDIVDPSFTSKMEQTLDD
IADGEVEWLPYLKKFYLGDKGLETLVRERESLIDASKARTVELENLDAKVRIGKYGPYIEVENGDGVVTASIPKDLTPAD
LDPKQVEIILRQKTVGPDQLGRHPETGEPIYVKIGAYGPYVQLGDKSDENPKPKQASLPKGVTPENITLEKAIGLLSLPR
TLGTHPGTGGKIQASLGRFGPYVVHDQGKEGKDYRSLKASDDVLTISLERALELISEPKKTRGSTSSKSKAALRELGTHP
EDDAPINIYDGPYGPYIKHGKTNVGLPEGTSMEDVTLATALELLSAKTATAKTTRKTSKTSTTKSKSTAKSSNAATKKKG
A
>Mature_880_residues
STLVIVESPTKARTIRNYLPKDYRVEASMGHVRDLPQSASEIPASVKGEAWAQLGVNVDADFEPVYVVPKDKKKVVTQLK
EALKDADELILATDEDREGESISWHLFQLLKPKVPTKRMVFHEITQEAIKKALKDCRNIDEQLVRAQETRRILDRLVGYT
LSPLLWKKIAWGLSAGRVQSVAVRLLVTKERQRRAFHEGTYWDLKAYLEQSKSPFTAQLTTLAGTKVATGSDFDASTGRI
TAGRNVVLLSETEAVALQERLTGKPWNVSEVEERPVTRKPAPPFTTSTLQQESNRKLRLSARDTMRIAQNLYEQGYITYM
RTDSVHLSEQAIAAARNCVEQLYGKQYLSPQKRQYTTKSKGAQEAHEAIRPAGSTFRTPQETGLGGRELALYDLIWKRTV
ACQMADSRQTQITVQLQVEDAGFRASGKRIDFPGYLRAYVEGSDDPEAALEDQEVILPSLKVGDHPDCQNIEAVGHETQP
PARYTEATLVKTLESEGIGRPSTYASIIGTIIDKGYSQLVNNALIPTFTAFAVTDLLEKNFPDIVDPSFTSKMEQTLDDI
ADGEVEWLPYLKKFYLGDKGLETLVRERESLIDASKARTVELENLDAKVRIGKYGPYIEVENGDGVVTASIPKDLTPADL
DPKQVEIILRQKTVGPDQLGRHPETGEPIYVKIGAYGPYVQLGDKSDENPKPKQASLPKGVTPENITLEKAIGLLSLPRT
LGTHPGTGGKIQASLGRFGPYVVHDQGKEGKDYRSLKASDDVLTISLERALELISEPKKTRGSTSSKSKAALRELGTHPE
DDAPINIYDGPYGPYIKHGKTNVGLPEGTSMEDVTLATALELLSAKTATAKTTRKTSKTSTTKSKSTAKSSNAATKKKGA

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=509, Percent_Identity=25.3438113948919, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI4507635, Length=518, Percent_Identity=22.972972972973, Blast_Score=96, Evalue=1e-19,
Organism=Escherichia coli, GI1787529, Length=589, Percent_Identity=40.4074702886248, Blast_Score=402, Evalue=1e-113,
Organism=Escherichia coli, GI1788061, Length=677, Percent_Identity=22.4519940915805, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI32563869, Length=593, Percent_Identity=25.1264755480607, Blast_Score=123, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17555378, Length=524, Percent_Identity=24.4274809160305, Blast_Score=118, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6323263, Length=586, Percent_Identity=23.3788395904437, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24585251, Length=537, Percent_Identity=26.4432029795158, Blast_Score=120, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 97190; Mature: 97059

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLVIVESPTKARTIRNYLPKDYRVEASMGHVRDLPQSASEIPASVKGEAWAQLGVNVD
CCEEEEEECCHHHHHHHHHCCCCEEEECCHHHHHHCCCHHHHCCCCCCCCHHHHCCCCCC
ADFEPVYVVPKDKKKVVTQLKEALKDADELILATDEDREGESISWHLFQLLKPKVPTKRM
CCCCCEEEECCCHHHHHHHHHHHHHCHHHEEEECCCCCCCCCHHHHHHHHHCCCCCHHHH
VFHEITQEAIKKALKDCRNIDEQLVRAQETRRILDRLVGYTLSPLLWKKIAWGLSAGRVQ
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
SVAVRLLVTKERQRRAFHEGTYWDLKAYLEQSKSPFTAQLTTLAGTKVATGSDFDASTGR
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCEEECCCCCCCCCCE
ITAGRNVVLLSETEAVALQERLTGKPWNVSEVEERPVTRKPAPPFTTSTLQQESNRKLRL
EECCCEEEEEECCHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHCCCCEEEE
SARDTMRIAQNLYEQGYITYMRTDSVHLSEQAIAAARNCVEQLYGKQYLSPQKRQYTTKS
EHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCHHC
KGAQEAHEAIRPAGSTFRTPQETGLGGRELALYDLIWKRTVACQMADSRQTQITVQLQVE
CCHHHHHHHHCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEE
DAGFRASGKRIDFPGYLRAYVEGSDDPEAALEDQEVILPSLKVGDHPDCQNIEAVGHETQ
CCCCCCCCCCCCCCHHHHHEECCCCCHHHHCCCCCEEECCCCCCCCCCCCCHHHCCCCCC
PPARYTEATLVKTLESEGIGRPSTYASIIGTIIDKGYSQLVNNALIPTFTAFAVTDLLEK
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
NFPDIVDPSFTSKMEQTLDDIADGEVEWLPYLKKFYLGDKGLETLVRERESLIDASKART
CCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCE
VELENLDAKVRIGKYGPYIEVENGDGVVTASIPKDLTPADLDPKQVEIILRQKTVGPDQL
EEEECCCCEEEECCCCCEEEEECCCEEEEECCCCCCCCCCCCHHHEEEEEHHCCCCHHHH
GRHPETGEPIYVKIGAYGPYVQLGDKSDENPKPKQASLPKGVTPENITLEKAIGLLSLPR
CCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHCCH
TLGTHPGTGGKIQASLGRFGPYVVHDQGKEGKDYRSLKASDDVLTISLERALELISEPKK
HHCCCCCCCCEEEECCCCCCCEEEECCCCCCCCHHHCCCCCCEEEEEHHHHHHHHHCCHH
TRGSTSSKSKAALRELGTHPEDDAPINIYDGPYGPYIKHGKTNVGLPEGTSMEDVTLATA
HCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
LELLSAKTATAKTTRKTSKTSTTKSKSTAKSSNAATKKKGA
HHHHHCCHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
STLVIVESPTKARTIRNYLPKDYRVEASMGHVRDLPQSASEIPASVKGEAWAQLGVNVD
CEEEEEECCHHHHHHHHHCCCCEEEECCHHHHHHCCCHHHHCCCCCCCCHHHHCCCCCC
ADFEPVYVVPKDKKKVVTQLKEALKDADELILATDEDREGESISWHLFQLLKPKVPTKRM
CCCCCEEEECCCHHHHHHHHHHHHHCHHHEEEECCCCCCCCCHHHHHHHHHCCCCCHHHH
VFHEITQEAIKKALKDCRNIDEQLVRAQETRRILDRLVGYTLSPLLWKKIAWGLSAGRVQ
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
SVAVRLLVTKERQRRAFHEGTYWDLKAYLEQSKSPFTAQLTTLAGTKVATGSDFDASTGR
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCEEECCCCCCCCCCE
ITAGRNVVLLSETEAVALQERLTGKPWNVSEVEERPVTRKPAPPFTTSTLQQESNRKLRL
EECCCEEEEEECCHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHCCCCEEEE
SARDTMRIAQNLYEQGYITYMRTDSVHLSEQAIAAARNCVEQLYGKQYLSPQKRQYTTKS
EHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCHHC
KGAQEAHEAIRPAGSTFRTPQETGLGGRELALYDLIWKRTVACQMADSRQTQITVQLQVE
CCHHHHHHHHCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEE
DAGFRASGKRIDFPGYLRAYVEGSDDPEAALEDQEVILPSLKVGDHPDCQNIEAVGHETQ
CCCCCCCCCCCCCCHHHHHEECCCCCHHHHCCCCCEEECCCCCCCCCCCCCHHHCCCCCC
PPARYTEATLVKTLESEGIGRPSTYASIIGTIIDKGYSQLVNNALIPTFTAFAVTDLLEK
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
NFPDIVDPSFTSKMEQTLDDIADGEVEWLPYLKKFYLGDKGLETLVRERESLIDASKART
CCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCE
VELENLDAKVRIGKYGPYIEVENGDGVVTASIPKDLTPADLDPKQVEIILRQKTVGPDQL
EEEECCCCEEEECCCCCEEEEECCCEEEEECCCCCCCCCCCCHHHEEEEEHHCCCCHHHH
GRHPETGEPIYVKIGAYGPYVQLGDKSDENPKPKQASLPKGVTPENITLEKAIGLLSLPR
CCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHCCH
TLGTHPGTGGKIQASLGRFGPYVVHDQGKEGKDYRSLKASDDVLTISLERALELISEPKK
HHCCCCCCCCEEEECCCCCCCEEEECCCCCCCCHHHCCCCCCEEEEEHHHHHHHHHCCHH
TRGSTSSKSKAALRELGTHPEDDAPINIYDGPYGPYIKHGKTNVGLPEGTSMEDVTLATA
HCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
LELLSAKTATAKTTRKTSKTSTTKSKSTAKSSNAATKKKGA
HHHHHCCHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]