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The map label for this gene is gldA [C]

Identifier: 17230256

GI number: 17230256

Start: 3360065

End: 3361255

Strand: Direct

Name: gldA [C]

Synonym: alr2764

Alternate gene names: 17230256

Gene position: 3360065-3361255 (Clockwise)

Preceding gene: 17230255

Following gene: 17230257

Centisome position: 52.39

GC content: 45.93

Gene sequence:

>1191_bases
ATGCCTAATCAATTATCTAATCAAACCTTGTCTAATCAAACTGCTAGTTCATTAATCACCCTCGCGGTTGCCCCGGCAAA
AGTTATCCGTGGCTGTAATATATTGCAGGTAGCCGCCACAGAAATTGCCAAATTGGGCAGTCGTCCTTTAATTGTGGCAG
GTAATAGCACCGTTGCTATCAGCCAAGAGAAACTACAGCCAATTTTAGAAGCCCAGAATCTGCACGTTGCCCAAGCCTCT
TATGGTGCAGATTGTGCGGAAGTTAGCTTAAAAGCCTTGAGGAAAGCAGCCAAAGAACATAAAGCCGATACGATTATCGG
GATTGGTGGGGGCAAAGCTTTAGATACAGCAAAGTTAGTTGCCCATCAGTTAGATATACCTATAGTCACAATTCCCACAT
CGGCAGCTACCTGTGCCGCTTGGACGGCGCTATCAAATGTATATTCTGAGGACGGGGCGTTTCTTTACGATGTGGCTTTA
TCTCGCTGTCCCGACTTGCTGATACTTGACTACGACTTGATCCAAACTGCACCACAACATACACTAGTGGCAGGGATTGG
GGATGCGATCGCTAAATGGTATGAAGCTTCTGTTAGTAGTGGGCATTTAGAACAAACTTTAATTATCTCCGCAGTCCAAC
AAGCGCGAGTTTTACGCGATATTTTATTCCAAAAGTCGGCAATTGCCTTACAACAACCAGGTAGTGAAGCTTGGCGGGAA
GTTGTTGATGCCACAGTTTTACTAGCTGGTGTCATCGGTGGACTCGGTGGGGCGCAGTGCCGTACAGTAGCCGCCCATGC
AGTCCATAATGGTTTAACCCACATTGCCGGACACAGTAGTATCCACGGTGAAAAAGTTGCCTACGGTATTTTGGTACAGC
TGCGTCTGGAAGAAATGGTACAAGGTAATCAATTAGCAGCCGCCTCACGACAACAACTATTAAAGTTTTATGCAGAGATT
GGACTGCCACAAAAATTAGGAGACTTGGGGTTGGGTAATATTACCTTAGGCGAGTTACAAACAGCCGCCGAAATCGCTTT
AACACCTCAATCTGACATTCATCGTCTACCATTCAAAGTTGTGTTGGAACAGTTAATGGCAGCGATGGTTTCTACCACTG
CACCAACAGATAGTAGAGACTCAGTTCATCGTGTCTCCACCAGGGGAATAAGTGACGAGGTTGAGGAATGA

Upstream 100 bases:

>100_bases
ATGTTCTGACAAGCGTTTCCCTTCTCTGGGGAAACTGATATAAGTTCTAATTAGTCTAATTTTATTAAGATATATCAAGG
TTTTTGGCAAACTAGAGTAT

Downstream 100 bases:

>100_bases
GTTTCGATTGGATTACGCCAGCAGATAGGATACAGCAACTACCACCTTATGTATTTGCCCGTCTTGACGAGCTGAAAGCT
AAGGCAAGGGAGCAAGGAAT

Product: glycerol dehydrogenase

Products: glycerone; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MPNQLSNQTLSNQTASSLITLAVAPAKVIRGCNILQVAATEIAKLGSRPLIVAGNSTVAISQEKLQPILEAQNLHVAQAS
YGADCAEVSLKALRKAAKEHKADTIIGIGGGKALDTAKLVAHQLDIPIVTIPTSAATCAAWTALSNVYSEDGAFLYDVAL
SRCPDLLILDYDLIQTAPQHTLVAGIGDAIAKWYEASVSSGHLEQTLIISAVQQARVLRDILFQKSAIALQQPGSEAWRE
VVDATVLLAGVIGGLGGAQCRTVAAHAVHNGLTHIAGHSSIHGEKVAYGILVQLRLEEMVQGNQLAAASRQQLLKFYAEI
GLPQKLGDLGLGNITLGELQTAAEIALTPQSDIHRLPFKVVLEQLMAAMVSTTAPTDSRDSVHRVSTRGISDEVEE

Sequences:

>Translated_396_residues
MPNQLSNQTLSNQTASSLITLAVAPAKVIRGCNILQVAATEIAKLGSRPLIVAGNSTVAISQEKLQPILEAQNLHVAQAS
YGADCAEVSLKALRKAAKEHKADTIIGIGGGKALDTAKLVAHQLDIPIVTIPTSAATCAAWTALSNVYSEDGAFLYDVAL
SRCPDLLILDYDLIQTAPQHTLVAGIGDAIAKWYEASVSSGHLEQTLIISAVQQARVLRDILFQKSAIALQQPGSEAWRE
VVDATVLLAGVIGGLGGAQCRTVAAHAVHNGLTHIAGHSSIHGEKVAYGILVQLRLEEMVQGNQLAAASRQQLLKFYAEI
GLPQKLGDLGLGNITLGELQTAAEIALTPQSDIHRLPFKVVLEQLMAAMVSTTAPTDSRDSVHRVSTRGISDEVEE
>Mature_395_residues
PNQLSNQTLSNQTASSLITLAVAPAKVIRGCNILQVAATEIAKLGSRPLIVAGNSTVAISQEKLQPILEAQNLHVAQASY
GADCAEVSLKALRKAAKEHKADTIIGIGGGKALDTAKLVAHQLDIPIVTIPTSAATCAAWTALSNVYSEDGAFLYDVALS
RCPDLLILDYDLIQTAPQHTLVAGIGDAIAKWYEASVSSGHLEQTLIISAVQQARVLRDILFQKSAIALQQPGSEAWREV
VDATVLLAGVIGGLGGAQCRTVAAHAVHNGLTHIAGHSSIHGEKVAYGILVQLRLEEMVQGNQLAAASRQQLLKFYAEIG
LPQKLGDLGLGNITLGELQTAAEIALTPQSDIHRLPFKVVLEQLMAAMVSTTAPTDSRDSVHRVSTRGISDEVEE

Specific function: Glycerol utilization. [C]

COG id: COG0371

COG function: function code C; Glycerol dehydrogenase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI87082352, Length=358, Percent_Identity=32.4022346368715, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1786815, Length=331, Percent_Identity=36.2537764350453, Blast_Score=155, Evalue=4e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR016205 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.1.6

Molecular weight: Translated: 41805; Mature: 41674

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00913 ADH_IRON_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNQLSNQTLSNQTASSLITLAVAPAKVIRGCNILQVAATEIAKLGSRPLIVAGNSTVAI
CCCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCCEEEE
SQEKLQPILEAQNLHVAQASYGADCAEVSLKALRKAAKEHKADTIIGIGGGKALDTAKLV
EHHHHHHHHHHCCCEEEHHHCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHH
AHQLDIPIVTIPTSAATCAAWTALSNVYSEDGAFLYDVALSRCPDLLILDYDLIQTAPQH
HHHCCCCEEEECCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHCCCEEEECHHHHHCCCCH
TLVAGIGDAIAKWYEASVSSGHLEQTLIISAVQQARVLRDILFQKSAIALQQPGSEAWRE
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
VVDATVLLAGVIGGLGGAQCRTVAAHAVHNGLTHIAGHSSIHGEKVAYGILVQLRLEEMV
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
QGNQLAAASRQQLLKFYAEIGLPQKLGDLGLGNITLGELQTAAEIALTPQSDIHRLPFKV
CCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCEEHHHHHHHHHEEECCHHHHHHCCHHH
VLEQLMAAMVSTTAPTDSRDSVHRVSTRGISDEVEE
HHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHCCC
>Mature Secondary Structure 
PNQLSNQTLSNQTASSLITLAVAPAKVIRGCNILQVAATEIAKLGSRPLIVAGNSTVAI
CCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCCEEEE
SQEKLQPILEAQNLHVAQASYGADCAEVSLKALRKAAKEHKADTIIGIGGGKALDTAKLV
EHHHHHHHHHHCCCEEEHHHCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHH
AHQLDIPIVTIPTSAATCAAWTALSNVYSEDGAFLYDVALSRCPDLLILDYDLIQTAPQH
HHHCCCCEEEECCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHCCCEEEECHHHHHCCCCH
TLVAGIGDAIAKWYEASVSSGHLEQTLIISAVQQARVLRDILFQKSAIALQQPGSEAWRE
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
VVDATVLLAGVIGGLGGAQCRTVAAHAVHNGLTHIAGHSSIHGEKVAYGILVQLRLEEMV
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
QGNQLAAASRQQLLKFYAEIGLPQKLGDLGLGNITLGELQTAAEIALTPQSDIHRLPFKV
CCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCEEHHHHHHHHHEEECCHHHHHHCCHHH
VLEQLMAAMVSTTAPTDSRDSVHRVSTRGISDEVEE
HHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+; NADH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.13 {NAD+}} 0.12 {NADH}} 0.06 {dihydroxyacetone}} 0.5 {glycerol}} [C]

Substrates: glycerol; NAD+

Specific reaction: glycerol + NAD+ = glycerone + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: Chelatingagents; Li+; Na+; Thiolcompounds [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]