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The map label for this gene is sir [H]

Identifier: 17228843

GI number: 17228843

Start: 1601399

End: 1603351

Strand: Direct

Name: sir [H]

Synonym: alr1348

Alternate gene names: 17228843

Gene position: 1601399-1603351 (Clockwise)

Preceding gene: 17228842

Following gene: 17228844

Centisome position: 24.97

GC content: 45.57

Gene sequence:

>1953_bases
ATGGTTAAATCTGCTCCTCCTCCAACCACTGGCCGTAAACCTTCTAAAGTCGAAGGGCTTAAAGAAAACAGTAATTTTTT
ACGTGAACCTGTAGCAACGCAAATCCTTGAGGATACTACTCATTTTACTGAAGATGCGGTACAAATCCTGAAGTTTCACG
GTTCTTATCAACAAGATAATCGTGACAATCGCACCAAAGGTCAAGAGAAAGATTACCAGTTTATGCTGCGGACAAAAAAT
CCGGGTGGCTTGGTTCCGCCGCAGCTTTACTTGGCTTTAGACAAGCTAGCTGATGAATATGGCAACCATACCTTACGAGC
TACCACTCGTCAAGGTTTCCAAATGCACGGGATTTTAAAGAAAAATCTCAAAGCAGCGATCGCTACAATTGTGAGAAACT
TAGGTTCAACTTTGGGTGCTTGTGGCGATATCAACCGCAACGTCATGGCTCCACCAGCCCCCTTCAAAAACCGGGCTGAA
TACCAGTATGCTTGGGAATATGCCCAAAATATCGCTGACTTACTCTCACCCCAAACAGGTGCTTATTACGAAATCTGGCT
AGATGGAGAAAAAGCCATCAGTGCGGAAGAAAGTCCAGAAGTAAAAGCAGCTAGACAAAGCAATGGTAACGGGACAATCA
TTCACGACACCGAAGAACCCCTCTACGGCACTTATTATATGCCGCGTAAGTTTAAGGTGAGTGTGACTGTTCCAGGGGAT
AATTCCATTGATTTATATTCCCAAGACCTGACTCTGGTTGTTCTGACTGATAAAAAAGGCAACCTAGAAGGATTTAATAT
CTTTGCTGGTGGTGGTTTAGGACGGACACACAACAAGGAAGAAACTTTTGCTCGATTGGCAGACCCCATTGGCTATGTAG
CCAAGGAAGATGTGTATAGTGCCGTCAAAGCTATTGTTGCGACTCAAAGAGATTATGGCGATCGCACTGACCGCCGTCAC
GCTCGGTTAAAATACTTAATCAATGATTGGGGTGTGGATAAGTTCCGCGCCAAGGTGGAAGAATATTTTGGTAAACCCAT
TGCCCCTTTCAAGCAACTACCAGAGTTTAAATATCACGATTTCCTGGGTTGGCATGAACAAGGCGATGGCAATTTATTTT
TAGGCATTTCCATCGATAACGGTCGGGTAAAAGATGAAGGCGCGTTTCAACTGAAAACCGCCTTACGGGAAATCGTCGAG
CAATTTAACCTACCCATCCGCCTGACACCCAACCAAAACCTGATTTTCTACGAAATCACCCCAGAGAACAAGCCAGCAAT
TCAAGCCATTCTCGACCGTTGCAATGTGGTTTCCGACCCCACCACCATTGAGCCTTTAGTCCGTTATGCAATGGCTTGTC
CAGCCCTACCTACCTGCGGTTTGGCAATCACAGAATCAGAAAGAGCCATCCCCGGCATTTTGGAGAGGATTCGTACTCTC
TTAGATAAATTGGGTTTACAAAATGAACATTTTGTGGTAAGAATGACAGGCTGCCCCAACGGTTGCGCCCGTCCTTACAT
GGCAGAACTAGGCTTTGTTGGTAGCGCGCCGGAAAGTTACCAAGTGTGGTTAGGGGGTTCACCCAATCAAACACGCTTGG
CACAACCTTATGTAGAGAAACTGCACGATAACGACATAGAAAGCTTCCTAGAACCGATTTTTGTGTATTTCCGCAAATAT
CGGAAGTCTGGGGAAAGTTTTGGCGATTTTTGCGATCGCGTCGGCTTTGATGCCATTCGTGAATTTGTTGCCGAGTATCA
ACCGGAAACAACACCAGTCACCATAGAACCAGAACAGCCTGAAGTTGTATCTAAGTCACGTAAAGCTCGGTATCGGGTCA
GCCTGCACGATGATTTGTATAACAAAGTGAAGGCGACAGCGACAAGTCAAGGCCGGTCGATGACTGATTTAGTCAAAGAT
GCCCTAGAGGCTTACTTCCAAAGCCTTTCTTGA

Upstream 100 bases:

>100_bases
ATTTCCGACCGGATTACCGGGAAACTTTCAGGCGAAAAGCACTGGAGGTTTGCTGCTTCGGCGATTACCGATGCCTTATT
GAGAATCCTATAGAACTCTG

Downstream 100 bases:

>100_bases
AGAAGGGGTCAAATACAGACCTAACCCCCAGCCCCTTCCCTTGTAGGGAAGGGGAGTAAGAATAAAATTTCATGAGCCAA
TTACAACGAATTACGATCGC

Product: sulfite reductase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 650; Mature: 650

Protein sequence:

>650_residues
MVKSAPPPTTGRKPSKVEGLKENSNFLREPVATQILEDTTHFTEDAVQILKFHGSYQQDNRDNRTKGQEKDYQFMLRTKN
PGGLVPPQLYLALDKLADEYGNHTLRATTRQGFQMHGILKKNLKAAIATIVRNLGSTLGACGDINRNVMAPPAPFKNRAE
YQYAWEYAQNIADLLSPQTGAYYEIWLDGEKAISAEESPEVKAARQSNGNGTIIHDTEEPLYGTYYMPRKFKVSVTVPGD
NSIDLYSQDLTLVVLTDKKGNLEGFNIFAGGGLGRTHNKEETFARLADPIGYVAKEDVYSAVKAIVATQRDYGDRTDRRH
ARLKYLINDWGVDKFRAKVEEYFGKPIAPFKQLPEFKYHDFLGWHEQGDGNLFLGISIDNGRVKDEGAFQLKTALREIVE
QFNLPIRLTPNQNLIFYEITPENKPAIQAILDRCNVVSDPTTIEPLVRYAMACPALPTCGLAITESERAIPGILERIRTL
LDKLGLQNEHFVVRMTGCPNGCARPYMAELGFVGSAPESYQVWLGGSPNQTRLAQPYVEKLHDNDIESFLEPIFVYFRKY
RKSGESFGDFCDRVGFDAIREFVAEYQPETTPVTIEPEQPEVVSKSRKARYRVSLHDDLYNKVKATATSQGRSMTDLVKD
ALEAYFQSLS

Sequences:

>Translated_650_residues
MVKSAPPPTTGRKPSKVEGLKENSNFLREPVATQILEDTTHFTEDAVQILKFHGSYQQDNRDNRTKGQEKDYQFMLRTKN
PGGLVPPQLYLALDKLADEYGNHTLRATTRQGFQMHGILKKNLKAAIATIVRNLGSTLGACGDINRNVMAPPAPFKNRAE
YQYAWEYAQNIADLLSPQTGAYYEIWLDGEKAISAEESPEVKAARQSNGNGTIIHDTEEPLYGTYYMPRKFKVSVTVPGD
NSIDLYSQDLTLVVLTDKKGNLEGFNIFAGGGLGRTHNKEETFARLADPIGYVAKEDVYSAVKAIVATQRDYGDRTDRRH
ARLKYLINDWGVDKFRAKVEEYFGKPIAPFKQLPEFKYHDFLGWHEQGDGNLFLGISIDNGRVKDEGAFQLKTALREIVE
QFNLPIRLTPNQNLIFYEITPENKPAIQAILDRCNVVSDPTTIEPLVRYAMACPALPTCGLAITESERAIPGILERIRTL
LDKLGLQNEHFVVRMTGCPNGCARPYMAELGFVGSAPESYQVWLGGSPNQTRLAQPYVEKLHDNDIESFLEPIFVYFRKY
RKSGESFGDFCDRVGFDAIREFVAEYQPETTPVTIEPEQPEVVSKSRKARYRVSLHDDLYNKVKATATSQGRSMTDLVKD
ALEAYFQSLS
>Mature_650_residues
MVKSAPPPTTGRKPSKVEGLKENSNFLREPVATQILEDTTHFTEDAVQILKFHGSYQQDNRDNRTKGQEKDYQFMLRTKN
PGGLVPPQLYLALDKLADEYGNHTLRATTRQGFQMHGILKKNLKAAIATIVRNLGSTLGACGDINRNVMAPPAPFKNRAE
YQYAWEYAQNIADLLSPQTGAYYEIWLDGEKAISAEESPEVKAARQSNGNGTIIHDTEEPLYGTYYMPRKFKVSVTVPGD
NSIDLYSQDLTLVVLTDKKGNLEGFNIFAGGGLGRTHNKEETFARLADPIGYVAKEDVYSAVKAIVATQRDYGDRTDRRH
ARLKYLINDWGVDKFRAKVEEYFGKPIAPFKQLPEFKYHDFLGWHEQGDGNLFLGISIDNGRVKDEGAFQLKTALREIVE
QFNLPIRLTPNQNLIFYEITPENKPAIQAILDRCNVVSDPTTIEPLVRYAMACPALPTCGLAITESERAIPGILERIRTL
LDKLGLQNEHFVVRMTGCPNGCARPYMAELGFVGSAPESYQVWLGGSPNQTRLAQPYVEKLHDNDIESFLEPIFVYFRKY
RKSGESFGDFCDRVGFDAIREFVAEYQPETTPVTIEPEQPEVVSKSRKARYRVSLHDDLYNKVKATATSQGRSMTDLVKD
ALEAYFQSLS

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=583, Percent_Identity=36.8782161234991, Blast_Score=375, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6322597, Length=589, Percent_Identity=40.9168081494058, Blast_Score=429, Evalue=1e-121,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR011787 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.7.1 [H]

Molecular weight: Translated: 73190; Mature: 73190

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKSAPPPTTGRKPSKVEGLKENSNFLREPVATQILEDTTHFTEDAVQILKFHGSYQQDN
CCCCCCCCCCCCCCCHHCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
RDNRTKGQEKDYQFMLRTKNPGGLVPPQLYLALDKLADEYGNHTLRATTRQGFQMHGILK
CCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEHHHCCCHHHHHHH
KNLKAAIATIVRNLGSTLGACGDINRNVMAPPAPFKNRAEYQYAWEYAQNIADLLSPQTG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
AYYEIWLDGEKAISAEESPEVKAARQSNGNGTIIHDTEEPLYGTYYMPRKFKVSVTVPGD
CEEEEEECCCHHCCCCCCCCHHHHHCCCCCCEEEECCCCCCEEEEECCCEEEEEEEECCC
NSIDLYSQDLTLVVLTDKKGNLEGFNIFAGGGLGRTHNKEETFARLADPIGYVAKEDVYS
CCCEEEECCEEEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AVKAIVATQRDYGDRTDRRHARLKYLINDWGVDKFRAKVEEYFGKPIAPFKQLPEFKYHD
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHCCCCCHHH
FLGWHEQGDGNLFLGISIDNGRVKDEGAFQLKTALREIVEQFNLPIRLTPNQNLIFYEIT
CCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEEC
PENKPAIQAILDRCNVVSDPTTIEPLVRYAMACPALPTCGLAITESERAIPGILERIRTL
CCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHH
LDKLGLQNEHFVVRMTGCPNGCARPYMAELGFVGSAPESYQVWLGGSPNQTRLAQPYVEK
HHHHCCCCCCEEEEECCCCCCCCCCHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHH
LHDNDIESFLEPIFVYFRKYRKSGESFGDFCDRVGFDAIREFVAEYQPETTPVTIEPEQP
HCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCEEECCCCC
EVVSKSRKARYRVSLHDDLYNKVKATATSQGRSMTDLVKDALEAYFQSLS
HHHHHCCCCEEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVKSAPPPTTGRKPSKVEGLKENSNFLREPVATQILEDTTHFTEDAVQILKFHGSYQQDN
CCCCCCCCCCCCCCCHHCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
RDNRTKGQEKDYQFMLRTKNPGGLVPPQLYLALDKLADEYGNHTLRATTRQGFQMHGILK
CCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEHHHCCCHHHHHHH
KNLKAAIATIVRNLGSTLGACGDINRNVMAPPAPFKNRAEYQYAWEYAQNIADLLSPQTG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
AYYEIWLDGEKAISAEESPEVKAARQSNGNGTIIHDTEEPLYGTYYMPRKFKVSVTVPGD
CEEEEEECCCHHCCCCCCCCHHHHHCCCCCCEEEECCCCCCEEEEECCCEEEEEEEECCC
NSIDLYSQDLTLVVLTDKKGNLEGFNIFAGGGLGRTHNKEETFARLADPIGYVAKEDVYS
CCCEEEECCEEEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AVKAIVATQRDYGDRTDRRHARLKYLINDWGVDKFRAKVEEYFGKPIAPFKQLPEFKYHD
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHCCCCCHHH
FLGWHEQGDGNLFLGISIDNGRVKDEGAFQLKTALREIVEQFNLPIRLTPNQNLIFYEIT
CCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEEC
PENKPAIQAILDRCNVVSDPTTIEPLVRYAMACPALPTCGLAITESERAIPGILERIRTL
CCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHH
LDKLGLQNEHFVVRMTGCPNGCARPYMAELGFVGSAPESYQVWLGGSPNQTRLAQPYVEK
HHHHCCCCCCEEEEECCCCCCCCCCHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHH
LHDNDIESFLEPIFVYFRKYRKSGESFGDFCDRVGFDAIREFVAEYQPETTPVTIEPEQP
HCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCEEECCCCC
EVVSKSRKARYRVSLHDDLYNKVKATATSQGRSMTDLVKDALEAYFQSLS
HHHHHCCCCEEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]