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The map label for this gene is thiE [H]

Identifier: 17228838

GI number: 17228838

Start: 1595159

End: 1596298

Strand: Direct

Name: thiE [H]

Synonym: alr1343

Alternate gene names: 17228838

Gene position: 1595159-1596298 (Clockwise)

Preceding gene: 17228836

Following gene: 17228839

Centisome position: 24.87

GC content: 46.84

Gene sequence:

>1140_bases
ATGCAAATCCATGATGCGAGAAACATTGCAAATATGAAAGAGGCTGGCTATTACGATGAAAGCATCACTAATGGGGTAGT
TGTAATGGTGGAACCATACAGCCAACAAAAGCAAATCCAGCAGGTTGTTTACCGTATATTAGATGCTAATTTAGACCGCG
CTCGTGAAGGGTTGCGAATTATTGAAGAATGGTGTCGCTTTGGGTTGAATAGCGGCCAGTTGGCAGGGGAATGTAAGTAC
TTGCGCCAAGAGGTGGCTGTTTGGCACACAGAAGAATTGCGCGCAGCCAGAGATACAGCAGGTGATCCCGGCACTGATTT
GAGCCATCCACAGGAGGAACAACGCTCTAGTATTAAGGCGTTGTTACAAGCTAACTTTTGCCGTGTGGAGGAAGCTTTGC
GGGTGTTGGAGGAATACGGTAAGCTTTATCACCCGAAAATGGGACAGGCTTGTAAGCAGATGCGATATCGAGTTTACAGC
CTGGAAACTAATTTGATGGGTCATCAGCGCCATCAACTGCTACGGCGATCGCGTTTATATCTTGTCACTTCCCCATCGGA
AAGTTTGTTACCAACGGTGGAAGCTGCTCTCAAAGGTGGCTTGACATTGTTACAATATCGTGACAAAGACACTGATGATT
TTGTGCGTTTGGAACTGGCGACCAAACTCCGCCAACTCTGTCACAGCTACGGTGCTTTATTTATTATCAATGACAGGGTG
GATTTGGCTCTGGCTGTGGATGCTGATGGTGTGCATCTAGGTCAACAAGATATGCCTATCGCTACTGCTAGACAATTACT
AGGCCCCCAACGTCTCATCGGTCGTTCTACTACCAACGCTGATGAAATGCAAAAGGCGATCGCTGAAGGTGCAGACTATA
TAGGTGTGGGGCCAGTATACGAAACTCCCACCAAAATAGGTAAGGCGGCAGCTGGTTTAGGATATGTGAGTTATGCGGCT
CAACATAGTTCAGTTCCCTGGTTTGCTATTGGGGGCATTGATGCCAATAATATCAATGATGTGATTGATGCGGGAGCAGA
AAGAGTGGCGGTAGTGCGATCGCTCATGCAGGCGGAACAACCTACCCTAGTCACACAATATTTGATTTCTCAACTCAATC
GCATCAAGCCAGAAAGTTAA

Upstream 100 bases:

>100_bases
GGGGACTAGGGACAAATTACATTTTCTTTCATTACCTAATCCCCGATACCTAATCCCCGATACCCAATCCCCGATACCCA
ATACCCAATCCCCAATACCT

Downstream 100 bases:

>100_bases
TCAACTGACAATTTAAAGTTTATCAAGACATCTGGCAGCCAATTGGTAAAAGATGTCTAATTTTTGTGTATCTTAATTAC
GACTTACGAAATTATTCTCA

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MQIHDARNIANMKEAGYYDESITNGVVVMVEPYSQQKQIQQVVYRILDANLDRAREGLRIIEEWCRFGLNSGQLAGECKY
LRQEVAVWHTEELRAARDTAGDPGTDLSHPQEEQRSSIKALLQANFCRVEEALRVLEEYGKLYHPKMGQACKQMRYRVYS
LETNLMGHQRHQLLRRSRLYLVTSPSESLLPTVEAALKGGLTLLQYRDKDTDDFVRLELATKLRQLCHSYGALFIINDRV
DLALAVDADGVHLGQQDMPIATARQLLGPQRLIGRSTTNADEMQKAIAEGADYIGVGPVYETPTKIGKAAAGLGYVSYAA
QHSSVPWFAIGGIDANNINDVIDAGAERVAVVRSLMQAEQPTLVTQYLISQLNRIKPES

Sequences:

>Translated_379_residues
MQIHDARNIANMKEAGYYDESITNGVVVMVEPYSQQKQIQQVVYRILDANLDRAREGLRIIEEWCRFGLNSGQLAGECKY
LRQEVAVWHTEELRAARDTAGDPGTDLSHPQEEQRSSIKALLQANFCRVEEALRVLEEYGKLYHPKMGQACKQMRYRVYS
LETNLMGHQRHQLLRRSRLYLVTSPSESLLPTVEAALKGGLTLLQYRDKDTDDFVRLELATKLRQLCHSYGALFIINDRV
DLALAVDADGVHLGQQDMPIATARQLLGPQRLIGRSTTNADEMQKAIAEGADYIGVGPVYETPTKIGKAAAGLGYVSYAA
QHSSVPWFAIGGIDANNINDVIDAGAERVAVVRSLMQAEQPTLVTQYLISQLNRIKPES
>Mature_379_residues
MQIHDARNIANMKEAGYYDESITNGVVVMVEPYSQQKQIQQVVYRILDANLDRAREGLRIIEEWCRFGLNSGQLAGECKY
LRQEVAVWHTEELRAARDTAGDPGTDLSHPQEEQRSSIKALLQANFCRVEEALRVLEEYGKLYHPKMGQACKQMRYRVYS
LETNLMGHQRHQLLRRSRLYLVTSPSESLLPTVEAALKGGLTLLQYRDKDTDDFVRLELATKLRQLCHSYGALFIINDRV
DLALAVDADGVHLGQQDMPIATARQLLGPQRLIGRSTTNADEMQKAIAEGADYIGVGPVYETPTKIGKAAAGLGYVSYAA
QHSSVPWFAIGGIDANNINDVIDAGAERVAVVRSLMQAEQPTLVTQYLISQLNRIKPES

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=197, Percent_Identity=32.48730964467, Blast_Score=65, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6325042, Length=180, Percent_Identity=38.3333333333333, Blast_Score=119, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR016229
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 42373; Mature: 42373

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIHDARNIANMKEAGYYDESITNGVVVMVEPYSQQKQIQQVVYRILDANLDRAREGLRI
CCCCCHHHHHHHHHCCCCCHHCCCCEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHH
IEEWCRFGLNSGQLAGECKYLRQEVAVWHTEELRAARDTAGDPGTDLSHPQEEQRSSIKA
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
LLQANFCRVEEALRVLEEYGKLYHPKMGQACKQMRYRVYSLETNLMGHQRHQLLRRSRLY
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
LVTSPSESLLPTVEAALKGGLTLLQYRDKDTDDFVRLELATKLRQLCHSYGALFIINDRV
EEECCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCC
DLALAVDADGVHLGQQDMPIATARQLLGPQRLIGRSTTNADEMQKAIAEGADYIGVGPVY
EEEEEECCCCCCCCCCCCCHHHHHHHHCHHHHHCCCCCCHHHHHHHHHCCCCEECCCCCC
ETPTKIGKAAAGLGYVSYAAQHSSVPWFAIGGIDANNINDVIDAGAERVAVVRSLMQAEQ
CCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHCCHHHHHHHHHHHHCCC
PTLVTQYLISQLNRIKPES
CHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQIHDARNIANMKEAGYYDESITNGVVVMVEPYSQQKQIQQVVYRILDANLDRAREGLRI
CCCCCHHHHHHHHHCCCCCHHCCCCEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHH
IEEWCRFGLNSGQLAGECKYLRQEVAVWHTEELRAARDTAGDPGTDLSHPQEEQRSSIKA
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
LLQANFCRVEEALRVLEEYGKLYHPKMGQACKQMRYRVYSLETNLMGHQRHQLLRRSRLY
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
LVTSPSESLLPTVEAALKGGLTLLQYRDKDTDDFVRLELATKLRQLCHSYGALFIINDRV
EEECCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCC
DLALAVDADGVHLGQQDMPIATARQLLGPQRLIGRSTTNADEMQKAIAEGADYIGVGPVY
EEEEEECCCCCCCCCCCCCHHHHHHHHCHHHHHCCCCCCHHHHHHHHHCCCCEECCCCCC
ETPTKIGKAAAGLGYVSYAAQHSSVPWFAIGGIDANNINDVIDAGAERVAVVRSLMQAEQ
CCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHCCHHHHHHHHHHHHCCC
PTLVTQYLISQLNRIKPES
CHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]