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The map label for this gene is slt [H]

Identifier: 17228607

GI number: 17228607

Start: 1301997

End: 1304192

Strand: Direct

Name: slt [H]

Synonym: alr1112

Alternate gene names: 17228607

Gene position: 1301997-1304192 (Clockwise)

Preceding gene: 17228604

Following gene: 17228608

Centisome position: 20.3

GC content: 45.49

Gene sequence:

>2196_bases
ATGCTGAAAAAACTACAAAAAAAGCAAATTTCTATAATCGCTGGTGCGGCACTGTTTGCCTTTTCAGCTGGGGCGATGGT
GTCAGCACCTGAGATTGGTAAATTCTTGGGGCAATGGCTCAATGTGACTCAAAGTCAACCCCAAGATTTATCCGAAGCAA
GTAGGGCGAAATCAGATGTATTTCCACTGATATCACAATCGCCGACGGAACGAGCAGCCAAACTAGCAGAACTCTCGCAA
AACTCCCGATCGCCAGATCGAGAAAGAGCGCGTTATCTGTTGGCGAGTGATTATATTGACACCAAACAAGGCCAAAAAGC
CCTGGAATTACTCACGGGTTTAGAGAAAAGCTATCCTGTTCTGGCACCCTACATCTTACTAAAACAAGCACAAGCACAGG
ATTTATTAGGGGAAGATGGCAAAGCTTCTGACTTGAGGCGACAAGTATTAAAACAGTATCCCAAGGAAGCGGCGGCGGTA
AAAGCTATATATCTGATTGCTCAACCAAAGCTACAAGATACAGCGATCGCGCAATTTCCTTCCCATCCCCTCACCTGGGA
GATAATCAGGAAGCGCTTGTCAGAAAATCCCAATCAGCCACAGTTACGGTTAATCTTGGCTCAATATGCTTATACCCAGC
CAGGGATAGTAGGGGTGTTAGACGAGCTAGGAAAACAGACAAATCTCAAACCCCAAGATTGGGAAGTGATTGGTACAGCC
TACTGGGAGAACAATCAATTTCTCAAAGCGGCGAACGCTTACGCCAAAGCACCCAAAACAGCCCGTAACCTCTACCGGAC
TGCACGGGGATGGCAAGTAGGCGGGAAAAATAGAGAACAAGCAATCTCTACTTATAAGCAACTAGTACAGCAGTTTCCTG
ATGCACGGGAAACAGGACTTGGCTTGGTACGGTTGGCAGAAATGGCAAAAAGCAACAAAGATGCCCTACCCTATCTCAAC
CAAGTAATTGCTAAATTCCCTGAACAAGCAAGCCAGGCGTTGGTAAAAAAAGCCGAAATCCTCACAGCCCTTAAGGATGA
AAAAGCAGCCCAACAAACTTGGCAACAACTCATTACCAAATATGCCAAATCTAATGAAGCCGCCGAATATCGGTGGAAAA
TCGCTCTAGAGAAAGCCAAAGCCAGAGATTACACCAGCGCTTGGAAGTGGGCGCAACCAATTGTTGTCAACAACCCTAAC
AGTATTTTGGCTCCCAGGGCTGGTTTTTGGTTAGGTAGATGGGCAGCAACACTAGGAAAACAGCAGGAAGCTCAAACTGC
TTACGAGTACGTGATTAGCCAGTTTCCTTACTCTTATTATGCCTGGCGATCGGCAAATCTATTGGGGTTGAATGTCGGCA
ACTTTGATAACGTCCGTCAACTCACCCCAGAAGTAGTTCCTTATCAGCGCCCCATCCCTCCCACAGGTTCCCCAGCCTTC
CAAGAATTGTATTTACTGGGTCAAGATCGTGATGCGTGGTTGCAATGGGAGACAGAATATCTTAATAAACAAGAACCCAC
AGTAGCAGAACAATTCACCGAAGGCTTGATGCGGTTAGCGAGGGGTGAATATCTGTCAGGAATTAACCTGATTTCCAAAT
TAGAAGACAGGGAAACACCAGAAGAACAAGCCGAATATCAGGCCTTAAGTAAACAGATTACCTACTGGCAAGCCCGTTAC
CCGTTTCCCTACTTGAAAGAAATTGAAAAATGGTCATCTGAGCGAGAACTTAATCCCTTGCTAGTTACCGCCTTAATGCG
CCAAGAATCGCGGTTTGAAGCGAAAATCAAATCCGTTGTCGGCGCGACTGGCTTAATGCAGGTAATGCCCGATACAGCTA
AGTGGATAGCATCAAAAATTCCCTTAGATATCAAGACCATTAATTTAGAGAATCCCAATGACAACGTGATGCTAGGGACA
TGGTATTTAGATTATACCCATGAGAGGTATGGCAATAATTCCATGTTAGCGATCGCTAGTTACAATGCTGGCCCAGGTAG
CGTCGCCCGATGGTTGAAAACTCTACCCAATCAAGACCCAGATGAGTTTGTCGAAGCCATCCCCTTTAATGAAACCAGAG
ATTATGTCCGTCAGGTATTTGGTAACTATTGGAATTATCTGCGACTATATAATCCTGAAATTGCCAGCATCGTAGCTAAA
TATTCCGCCGAACAGCCGAAATTGCCGGAAAATTAG

Upstream 100 bases:

>100_bases
TTTTCCTAAAATCAGAAATTTCCAATGAAAAGGTGATTACACTCTCGGCAACTAATGCTAGAGTTTCAGGAAATTCATCG
GCACTGTTCAGCGAGTATCC

Downstream 100 bases:

>100_bases
GGAATTGGGGGTTAGGAATTTTATAGCAGTTGACTGTTGCCTGTTAATAGTTGACGGTTGACAGTTAACCGTCAACAAAC
CATATAAATGATATCCATTT

Product: hypothetical protein

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 731; Mature: 731

Protein sequence:

>731_residues
MLKKLQKKQISIIAGAALFAFSAGAMVSAPEIGKFLGQWLNVTQSQPQDLSEASRAKSDVFPLISQSPTERAAKLAELSQ
NSRSPDRERARYLLASDYIDTKQGQKALELLTGLEKSYPVLAPYILLKQAQAQDLLGEDGKASDLRRQVLKQYPKEAAAV
KAIYLIAQPKLQDTAIAQFPSHPLTWEIIRKRLSENPNQPQLRLILAQYAYTQPGIVGVLDELGKQTNLKPQDWEVIGTA
YWENNQFLKAANAYAKAPKTARNLYRTARGWQVGGKNREQAISTYKQLVQQFPDARETGLGLVRLAEMAKSNKDALPYLN
QVIAKFPEQASQALVKKAEILTALKDEKAAQQTWQQLITKYAKSNEAAEYRWKIALEKAKARDYTSAWKWAQPIVVNNPN
SILAPRAGFWLGRWAATLGKQQEAQTAYEYVISQFPYSYYAWRSANLLGLNVGNFDNVRQLTPEVVPYQRPIPPTGSPAF
QELYLLGQDRDAWLQWETEYLNKQEPTVAEQFTEGLMRLARGEYLSGINLISKLEDRETPEEQAEYQALSKQITYWQARY
PFPYLKEIEKWSSERELNPLLVTALMRQESRFEAKIKSVVGATGLMQVMPDTAKWIASKIPLDIKTINLENPNDNVMLGT
WYLDYTHERYGNNSMLAIASYNAGPGSVARWLKTLPNQDPDEFVEAIPFNETRDYVRQVFGNYWNYLRLYNPEIASIVAK
YSAEQPKLPEN

Sequences:

>Translated_731_residues
MLKKLQKKQISIIAGAALFAFSAGAMVSAPEIGKFLGQWLNVTQSQPQDLSEASRAKSDVFPLISQSPTERAAKLAELSQ
NSRSPDRERARYLLASDYIDTKQGQKALELLTGLEKSYPVLAPYILLKQAQAQDLLGEDGKASDLRRQVLKQYPKEAAAV
KAIYLIAQPKLQDTAIAQFPSHPLTWEIIRKRLSENPNQPQLRLILAQYAYTQPGIVGVLDELGKQTNLKPQDWEVIGTA
YWENNQFLKAANAYAKAPKTARNLYRTARGWQVGGKNREQAISTYKQLVQQFPDARETGLGLVRLAEMAKSNKDALPYLN
QVIAKFPEQASQALVKKAEILTALKDEKAAQQTWQQLITKYAKSNEAAEYRWKIALEKAKARDYTSAWKWAQPIVVNNPN
SILAPRAGFWLGRWAATLGKQQEAQTAYEYVISQFPYSYYAWRSANLLGLNVGNFDNVRQLTPEVVPYQRPIPPTGSPAF
QELYLLGQDRDAWLQWETEYLNKQEPTVAEQFTEGLMRLARGEYLSGINLISKLEDRETPEEQAEYQALSKQITYWQARY
PFPYLKEIEKWSSERELNPLLVTALMRQESRFEAKIKSVVGATGLMQVMPDTAKWIASKIPLDIKTINLENPNDNVMLGT
WYLDYTHERYGNNSMLAIASYNAGPGSVARWLKTLPNQDPDEFVEAIPFNETRDYVRQVFGNYWNYLRLYNPEIASIVAK
YSAEQPKLPEN
>Mature_731_residues
MLKKLQKKQISIIAGAALFAFSAGAMVSAPEIGKFLGQWLNVTQSQPQDLSEASRAKSDVFPLISQSPTERAAKLAELSQ
NSRSPDRERARYLLASDYIDTKQGQKALELLTGLEKSYPVLAPYILLKQAQAQDLLGEDGKASDLRRQVLKQYPKEAAAV
KAIYLIAQPKLQDTAIAQFPSHPLTWEIIRKRLSENPNQPQLRLILAQYAYTQPGIVGVLDELGKQTNLKPQDWEVIGTA
YWENNQFLKAANAYAKAPKTARNLYRTARGWQVGGKNREQAISTYKQLVQQFPDARETGLGLVRLAEMAKSNKDALPYLN
QVIAKFPEQASQALVKKAEILTALKDEKAAQQTWQQLITKYAKSNEAAEYRWKIALEKAKARDYTSAWKWAQPIVVNNPN
SILAPRAGFWLGRWAATLGKQQEAQTAYEYVISQFPYSYYAWRSANLLGLNVGNFDNVRQLTPEVVPYQRPIPPTGSPAF
QELYLLGQDRDAWLQWETEYLNKQEPTVAEQFTEGLMRLARGEYLSGINLISKLEDRETPEEQAEYQALSKQITYWQARY
PFPYLKEIEKWSSERELNPLLVTALMRQESRFEAKIKSVVGATGLMQVMPDTAKWIASKIPLDIKTINLENPNDNVMLGT
WYLDYTHERYGNNSMLAIASYNAGPGSVARWLKTLPNQDPDEFVEAIPFNETRDYVRQVFGNYWNYLRLYNPEIASIVAK
YSAEQPKLPEN

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=163, Percent_Identity=39.2638036809816, Blast_Score=113, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 83012; Mature: 83012

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: PS00922 TRANSGLYCOSYLASE ; PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKLQKKQISIIAGAALFAFSAGAMVSAPEIGKFLGQWLNVTQSQPQDLSEASRAKSDV
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
FPLISQSPTERAAKLAELSQNSRSPDRERARYLLASDYIDTKQGQKALELLTGLEKSYPV
CHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCH
LAPYILLKQAQAQDLLGEDGKASDLRRQVLKQYPKEAAAVKAIYLIAQPKLQDTAIAQFP
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHEEECCCCCHHHHHHCC
SHPLTWEIIRKRLSENPNQPQLRLILAQYAYTQPGIVGVLDELGKQTNLKPQDWEVIGTA
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCEEEEEE
YWENNQFLKAANAYAKAPKTARNLYRTARGWQVGGKNREQAISTYKQLVQQFPDARETGL
EECCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCH
GLVRLAEMAKSNKDALPYLNQVIAKFPEQASQALVKKAEILTALKDEKAAQQTWQQLITK
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YAKSNEAAEYRWKIALEKAKARDYTSAWKWAQPIVVNNPNSILAPRAGFWLGRWAATLGK
HHCCCCCHHEEEEEHHHHHHHCCHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHCC
QQEAQTAYEYVISQFPYSYYAWRSANLLGLNVGNFDNVRQLTPEVVPYQRPIPPTGSPAF
HHHHHHHHHHHHHHCCCHHEEECCCCEEEEECCCCCHHHHCCHHHCCCCCCCCCCCCHHH
QELYLLGQDRDAWLQWETEYLNKQEPTVAEQFTEGLMRLARGEYLSGINLISKLEDRETP
HHHHHHCCCCCHHEEEEHHHCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCC
EEQAEYQALSKQITYWQARYPFPYLKEIEKWSSERELNPLLVTALMRQESRFEAKIKSVV
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GATGLMQVMPDTAKWIASKIPLDIKTINLENPNDNVMLGTWYLDYTHERYGNNSMLAIAS
HHHHHHHHCCHHHHHHHHHCCCEEEEEEECCCCCCEEEEEEEEEEHHHHCCCCCEEEEEE
YNAGPGSVARWLKTLPNQDPDEFVEAIPFNETRDYVRQVFGNYWNYLRLYNPEIASIVAK
CCCCCHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHEECCHHHHHHHHH
YSAEQPKLPEN
HCCCCCCCCCC
>Mature Secondary Structure
MLKKLQKKQISIIAGAALFAFSAGAMVSAPEIGKFLGQWLNVTQSQPQDLSEASRAKSDV
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
FPLISQSPTERAAKLAELSQNSRSPDRERARYLLASDYIDTKQGQKALELLTGLEKSYPV
CHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCH
LAPYILLKQAQAQDLLGEDGKASDLRRQVLKQYPKEAAAVKAIYLIAQPKLQDTAIAQFP
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHEEECCCCCHHHHHHCC
SHPLTWEIIRKRLSENPNQPQLRLILAQYAYTQPGIVGVLDELGKQTNLKPQDWEVIGTA
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCEEEEEE
YWENNQFLKAANAYAKAPKTARNLYRTARGWQVGGKNREQAISTYKQLVQQFPDARETGL
EECCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCH
GLVRLAEMAKSNKDALPYLNQVIAKFPEQASQALVKKAEILTALKDEKAAQQTWQQLITK
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YAKSNEAAEYRWKIALEKAKARDYTSAWKWAQPIVVNNPNSILAPRAGFWLGRWAATLGK
HHCCCCCHHEEEEEHHHHHHHCCHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHCC
QQEAQTAYEYVISQFPYSYYAWRSANLLGLNVGNFDNVRQLTPEVVPYQRPIPPTGSPAF
HHHHHHHHHHHHHHCCCHHEEECCCCEEEEECCCCCHHHHCCHHHCCCCCCCCCCCCHHH
QELYLLGQDRDAWLQWETEYLNKQEPTVAEQFTEGLMRLARGEYLSGINLISKLEDRETP
HHHHHHCCCCCHHEEEEHHHCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCC
EEQAEYQALSKQITYWQARYPFPYLKEIEKWSSERELNPLLVTALMRQESRFEAKIKSVV
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GATGLMQVMPDTAKWIASKIPLDIKTINLENPNDNVMLGTWYLDYTHERYGNNSMLAIAS
HHHHHHHHCCHHHHHHHHHCCCEEEEEEECCCCCCEEEEEEEEEEHHHHCCCCCEEEEEE
YNAGPGSVARWLKTLPNQDPDEFVEAIPFNETRDYVRQVFGNYWNYLRLYNPEIASIVAK
CCCCCHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHEECCHHHHHHHHH
YSAEQPKLPEN
HCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]