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The map label for this gene is mltF

Identifier: 172087672

GI number: 172087672

Start: 711318

End: 712805

Strand: Reverse

Name: mltF

Synonym: VF_0651

Alternate gene names: 172087672

Gene position: 712805-711318 (Counterclockwise)

Preceding gene: 59711262

Following gene: 59711255

Centisome position: 24.6

GC content: 39.38

Gene sequence:

>1488_bases
TTGAGCCGCATTCGACATCACCGATTCATACAATCTTGTTTGGTTATTTCTACCCTTTTAATTACCTTAACCGGTTGTCA
GGTAGAATCTGAACCCAAAACCAAACTAGAACAAATTAGAGAACGTGGCATCTTACGAGTCAGCACATTAAATAACCAAC
TTTCTTATTACATTGGTTCAAATGGTCCTACTGGATTAGATTATGATCTTGCTAAAGCATTTGCTGAAAAGTTAGAAGTA
AAGCTAGAGATCACCCCTGCGTATACTTATTCAGGCTTATTTCCTGCATTAGAGCGTAATGAAGTCGATATCATTGCAGC
CAACATGACAATCACCCCTGAACGCTTACAGAAATTTCGTCCAGGTCCTGTCTACTATTATGTCAGTCAACAAGTCGTTT
ATAAGAAAGGGAGTTGGCGACCACGCAACATAAAAAACCTAACTCAACTTGATGAAAACCTTACTATTGTTAAAGATTCA
AGTTTTGAAGGTACGCTTATAAAATTAAAAGAGAAATACCCAAATCTTGATTGGAATACCGCAGCAGATACTGATGTAAG
CGAGCTTTTGAAAAAAGTAGCAACAGGTGAAATACATTACACTTTAGCTGATTCGGTAGAGCTATCATTAACTCAACGTA
TTCATCCCGATCTGGCTGTTGCTTTCGAAGTTACAGAAGATCAGCCTGTCGCATGGTTCTTACAACAAACCGAAGATGAC
AGTTTACAAGCGTTATTAATTGAATTTTTTGGTGAACTAAAAGAAAGCGGCAAACTTGCTCTTCTTGAAGAAAAATACTT
TGGGCATGTAGAATCCTTTGATTATGTAGATACCCGTGCCTTTATCCGTGCATTAGAAAGCAAGCTGCCAAAATGGGAAC
CGCTATTTAAAAAATACGCGGGGGACTTTGATTGGCGATTCCTTGCTGCTCTTTCTTATCAAGAGTCTCATTGGAACCCA
CTTGCAAAATCACCAACAGGCGTTCGCGGTATGATGATGCTAACTCTGCCAACAGCCCAATCAGTGGGAGTGAAGAATCG
TTTAAACCCCGAGCAAAGTATTCGTGGTGGTGCAGAATATTTACGTCGAATAGTAAAACGAGTTCCAGATTCAATCACCG
AACATGAAAAAATTTGGTTCGCACTGGCTTCATATAACATCGGTTTTGGTCATATGATGGATGCAAGGCGTCTAACACAA
CGTTTAGGTGGCGATCCTGATTCATGGACTGATGTAAAAGACAATTTACCGCTGCTTCGTCAACAGCGTTATTACCGATA
CTTACGTTATGGTTTTGCTCGTGGTGATGAAGCGCAAAATTACGTTGAAAATATTAGACGTTATTATCAAAGTATTATTG
GTTACGAACAAGAACAAGCGAACAAGTTAAAACAAGAAGAACTGACAGTTGAAGATCTACAAGTTATAGATGTTCCTCTG
TCATCAGCGGAAGCCAGTGTTAGCCAAGCGATTGAGACCAAAAAGTAA

Upstream 100 bases:

>100_bases
CTCAATATCTAATTATTGTTGTAATTTGTTACCGCACTTTTGCCTCTTTATTTACCATTTGATATAACTACGCCTTAAAT
TTTAAAAAGAGCATCCTGAC

Downstream 100 bases:

>100_bases
TTTATAATGATGAATCTTATCTCCGATTCATCGTCATAATACGGTAACAATAGAGGCTTAATCTTCATTCCACATCATCT
ATTTTTATGCGCGTTTTACA

Product: putative transglycosylase

Products: NA

Alternate protein names: Murein lyase F

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MSRIRHHRFIQSCLVISTLLITLTGCQVESEPKTKLEQIRERGILRVSTLNNQLSYYIGSNGPTGLDYDLAKAFAEKLEV
KLEITPAYTYSGLFPALERNEVDIIAANMTITPERLQKFRPGPVYYYVSQQVVYKKGSWRPRNIKNLTQLDENLTIVKDS
SFEGTLIKLKEKYPNLDWNTAADTDVSELLKKVATGEIHYTLADSVELSLTQRIHPDLAVAFEVTEDQPVAWFLQQTEDD
SLQALLIEFFGELKESGKLALLEEKYFGHVESFDYVDTRAFIRALESKLPKWEPLFKKYAGDFDWRFLAALSYQESHWNP
LAKSPTGVRGMMMLTLPTAQSVGVKNRLNPEQSIRGGAEYLRRIVKRVPDSITEHEKIWFALASYNIGFGHMMDARRLTQ
RLGGDPDSWTDVKDNLPLLRQQRYYRYLRYGFARGDEAQNYVENIRRYYQSIIGYEQEQANKLKQEELTVEDLQVIDVPL
SSAEASVSQAIETKK

Sequences:

>Translated_495_residues
MSRIRHHRFIQSCLVISTLLITLTGCQVESEPKTKLEQIRERGILRVSTLNNQLSYYIGSNGPTGLDYDLAKAFAEKLEV
KLEITPAYTYSGLFPALERNEVDIIAANMTITPERLQKFRPGPVYYYVSQQVVYKKGSWRPRNIKNLTQLDENLTIVKDS
SFEGTLIKLKEKYPNLDWNTAADTDVSELLKKVATGEIHYTLADSVELSLTQRIHPDLAVAFEVTEDQPVAWFLQQTEDD
SLQALLIEFFGELKESGKLALLEEKYFGHVESFDYVDTRAFIRALESKLPKWEPLFKKYAGDFDWRFLAALSYQESHWNP
LAKSPTGVRGMMMLTLPTAQSVGVKNRLNPEQSIRGGAEYLRRIVKRVPDSITEHEKIWFALASYNIGFGHMMDARRLTQ
RLGGDPDSWTDVKDNLPLLRQQRYYRYLRYGFARGDEAQNYVENIRRYYQSIIGYEQEQANKLKQEELTVEDLQVIDVPL
SSAEASVSQAIETKK
>Mature_494_residues
SRIRHHRFIQSCLVISTLLITLTGCQVESEPKTKLEQIRERGILRVSTLNNQLSYYIGSNGPTGLDYDLAKAFAEKLEVK
LEITPAYTYSGLFPALERNEVDIIAANMTITPERLQKFRPGPVYYYVSQQVVYKKGSWRPRNIKNLTQLDENLTIVKDSS
FEGTLIKLKEKYPNLDWNTAADTDVSELLKKVATGEIHYTLADSVELSLTQRIHPDLAVAFEVTEDQPVAWFLQQTEDDS
LQALLIEFFGELKESGKLALLEEKYFGHVESFDYVDTRAFIRALESKLPKWEPLFKKYAGDFDWRFLAALSYQESHWNPL
AKSPTGVRGMMMLTLPTAQSVGVKNRLNPEQSIRGGAEYLRRIVKRVPDSITEHEKIWFALASYNIGFGHMMDARRLTQR
LGGDPDSWTDVKDNLPLLRQQRYYRYLRYGFARGDEAQNYVENIRRYYQSIIGYEQEQANKLKQEELTVEDLQVIDVPLS
SAEASVSQAIETKK

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG4623

COG function: function code M; Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family

Homologues:

Organism=Escherichia coli, GI171474010, Length=427, Percent_Identity=51.5222482435597, Blast_Score=452, Evalue=1e-128,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MLTF_VIBF1 (Q5E750)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_204034.2
- ProteinModelPortal:   Q5E750
- SMR:   Q5E750
- GeneID:   3277359
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_0651
- NMPDR:   fig|312309.3.peg.1152
- eggNOG:   COG4623
- HOGENOM:   HBG644469
- OMA:   KQRISER
- ProtClustDB:   PRK10859
- BioCyc:   VFIS312309:VF0651-MONOMER
- HAMAP:   MF_02016
- InterPro:   IPR008258
- InterPro:   IPR001638
- InterPro:   IPR000189
- SMART:   SM00062

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT

EC number: NA

Molecular weight: Translated: 56912; Mature: 56781

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00922 TRANSGLYCOSYLASE; PS00013 PROKAR_LIPOPROTEIN

Important sites: ACT_SITE 315-315

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIRHHRFIQSCLVISTLLITLTGCQVESEPKTKLEQIRERGILRVSTLNNQLSYYIGS
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEECCCEEEEECC
NGPTGLDYDLAKAFAEKLEVKLEITPAYTYSGLFPALERNEVDIIAANMTITPERLQKFR
CCCCCCCHHHHHHHHHHCEEEEEEECCEECCCCCCCCCCCCEEEEEECCEECHHHHHHCC
PGPVYYYVSQQVVYKKGSWRPRNIKNLTQLDENLTIVKDSSFEGTLIKLKEKYPNLDWNT
CCCEEEEECCEEEEECCCCCCCCHHHHHHHCCCEEEEECCCCCCEEEEEHHHCCCCCCCC
AADTDVSELLKKVATGEIHYTLADSVELSLTQRIHPDLAVAFEVTEDQPVAWFLQQTEDD
CCCCCHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCEEEEEEECCCCCEEEEEECCCCH
SLQALLIEFFGELKESGKLALLEEKYFGHVESFDYVDTRAFIRALESKLPKWEPLFKKYA
HHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHC
GDFDWRFLAALSYQESHWNPLAKSPTGVRGMMMLTLPTAQSVGVKNRLNPEQSIRGGAEY
CCCCHHHHHHHHHCHHCCCCCCCCCCCCCEEEEEEECCCHHCCCCCCCCHHHHHHHHHHH
LRRIVKRVPDSITEHEKIWFALASYNIGFGHMMDARRLTQRLGGDPDSWTDVKDNLPLLR
HHHHHHHCCHHHHCHHHEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
QQRYYRYLRYGFARGDEAQNYVENIRRYYQSIIGYEQEQANKLKQEELTVEDLQVIDVPL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHEEEECCC
SSAEASVSQAIETKK
CHHHHHHHHHHHCCC
>Mature Secondary Structure 
SRIRHHRFIQSCLVISTLLITLTGCQVESEPKTKLEQIRERGILRVSTLNNQLSYYIGS
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEECCCEEEEECC
NGPTGLDYDLAKAFAEKLEVKLEITPAYTYSGLFPALERNEVDIIAANMTITPERLQKFR
CCCCCCCHHHHHHHHHHCEEEEEEECCEECCCCCCCCCCCCEEEEEECCEECHHHHHHCC
PGPVYYYVSQQVVYKKGSWRPRNIKNLTQLDENLTIVKDSSFEGTLIKLKEKYPNLDWNT
CCCEEEEECCEEEEECCCCCCCCHHHHHHHCCCEEEEECCCCCCEEEEEHHHCCCCCCCC
AADTDVSELLKKVATGEIHYTLADSVELSLTQRIHPDLAVAFEVTEDQPVAWFLQQTEDD
CCCCCHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCEEEEEEECCCCCEEEEEECCCCH
SLQALLIEFFGELKESGKLALLEEKYFGHVESFDYVDTRAFIRALESKLPKWEPLFKKYA
HHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHC
GDFDWRFLAALSYQESHWNPLAKSPTGVRGMMMLTLPTAQSVGVKNRLNPEQSIRGGAEY
CCCCHHHHHHHHHCHHCCCCCCCCCCCCCEEEEEEECCCHHCCCCCCCCHHHHHHHHHHH
LRRIVKRVPDSITEHEKIWFALASYNIGFGHMMDARRLTQRLGGDPDSWTDVKDNLPLLR
HHHHHHHCCHHHHCHHHEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
QQRYYRYLRYGFARGDEAQNYVENIRRYYQSIIGYEQEQANKLKQEELTVEDLQVIDVPL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHEEEECCC
SSAEASVSQAIETKK
CHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA