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The map label for this gene is gltX

Identifier: 170756625

GI number: 170756625

Start: 1218299

End: 1219756

Strand: Reverse

Name: gltX

Synonym: CLD_3466

Alternate gene names: 170756625

Gene position: 1219756-1218299 (Counterclockwise)

Preceding gene: 170755072

Following gene: 170756219

Centisome position: 30.82

GC content: 30.45

Gene sequence:

>1458_bases
ATGACAAACAAAGTCAGAACTAGATTCGCACCAAGTCCAACAGGTTATATGCATGTTGGTAACTTAAGAACTGCTTTATA
TGCTTATTTAATAGCAAAGCATGATAATGGTGATTTTATATTAAGAATTGAGGATACAGATCAAGAAAGATTAGTAGAAG
GTGCTTTAGATGTTATATATAATACTCTAAAAATTACTGGTCTTAGTCATGATGAAGGTCCAGATATTGGCGGACCTGTT
GGTCCATATGTTCAAAGTGAAAGAAGAAATATATACATAGAATATGCTGAAAAGCTAATAGAAAAAGGAGAAGCATACTA
CTGTTTCTGTTCAAAAGAAAGATTAGATATGCTAAGAGCTAATTCAGAGGCTTTAAAAAGACCTTTCAGATATGACAAAC
ACTGCATAGATCTTTCTAAAGAAGAAATCGATAAAAAAATAGCTGAAGGTGTTCCATATGTTATAAGACAAAAAAATCCA
ACTACTGGATCAACTTCTTTCCATGATGAAATATATGGAGATATTTCCGTAGATAATTCAGAATTAGATGATATGATTTT
AATAAAATCCGATGGACTTCCAACATATAATTTTGCTAATGTAGTAGATGACCACCTTATGGGAATAACTCACGTAGTAC
GTGGAAGTGAATATTTGTCATCTTCTCCTAAATATAATAGACTATATGAAGCTTTTGGCTGGGATGTTCCGATATACGTT
CATTGCCCACCAATAATGAAAGATGAGCATCACAAATTAAGTAAGAGAAATGGAGATGCTTCTTTCGAAGATTTAATGGC
TAAAGGTTACTTAAAAGAAGCTATCCTAAACTATATAGCTTTACTGGGATGGAACCCAGGTGGAGAAAAAGAAGTATTTT
CTATGGAAGAATTAATAGAAGCCTTTAATTATAGAAATATAAATAAAGCTCCAGCAGTTTTTGATACAAAAAAATTAAAA
TGGATGAACGGAGAATATATAAGAGCTTTATCTTTAGATAAATTCCATGAAATGGCTCTTCCTTATTATGAAGAAGCTTT
GACTAGAGATTTAGATACTAAAAAAATAAGTGAACTTTTACACACTAGAGTAGAGGTTTTAAATGAAATTCCTGAACAAT
TAGATTTCTTTAATAATCTTCTTGAATACTCTCCGAAAATGTATATTCATAAAAAAATGAAAACAACTTATGAAAATTCA
TTAAAAAGCTTAGAAGAAGTTCTTCCTAAACTAGAAGCTCTAGAAAATTGGACTTTTGAAAATATTAAAGAAGTATGTAT
GAATTTAGTTAAAGAACTTGAAGTTAAAAACGGTGTAGTTTTATGGCCTATAAGAACAGCAGTTTCTGGAAAACAATTTA
CTCCTGGTGGAGCTTTTGAAATAGCTGATATATTAGGTAAGGAAGAAACTCTAGAAAGAATAAAAATAGGAATAGACAAA
CTTAAAGCTTTACAATAA

Upstream 100 bases:

>100_bases
AGGCCTTGTTATAAAATTTACTTATAAAATGAATTTTTACCTTTCGTCCAAGTGATGAAAGGTTTTATTTATAAATATTT
TTTAAATGGGAGTGAAAACA

Downstream 100 bases:

>100_bases
AATTATATTTTACACTTAAAAATTGTAATAAATATACACTAAGAAAAATCTCAGCTAATTATAGCTGAGATTTTATTTTT
TTATTTTTTTATTTTTTTTA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MTNKVRTRFAPSPTGYMHVGNLRTALYAYLIAKHDNGDFILRIEDTDQERLVEGALDVIYNTLKITGLSHDEGPDIGGPV
GPYVQSERRNIYIEYAEKLIEKGEAYYCFCSKERLDMLRANSEALKRPFRYDKHCIDLSKEEIDKKIAEGVPYVIRQKNP
TTGSTSFHDEIYGDISVDNSELDDMILIKSDGLPTYNFANVVDDHLMGITHVVRGSEYLSSSPKYNRLYEAFGWDVPIYV
HCPPIMKDEHHKLSKRNGDASFEDLMAKGYLKEAILNYIALLGWNPGGEKEVFSMEELIEAFNYRNINKAPAVFDTKKLK
WMNGEYIRALSLDKFHEMALPYYEEALTRDLDTKKISELLHTRVEVLNEIPEQLDFFNNLLEYSPKMYIHKKMKTTYENS
LKSLEEVLPKLEALENWTFENIKEVCMNLVKELEVKNGVVLWPIRTAVSGKQFTPGGAFEIADILGKEETLERIKIGIDK
LKALQ

Sequences:

>Translated_485_residues
MTNKVRTRFAPSPTGYMHVGNLRTALYAYLIAKHDNGDFILRIEDTDQERLVEGALDVIYNTLKITGLSHDEGPDIGGPV
GPYVQSERRNIYIEYAEKLIEKGEAYYCFCSKERLDMLRANSEALKRPFRYDKHCIDLSKEEIDKKIAEGVPYVIRQKNP
TTGSTSFHDEIYGDISVDNSELDDMILIKSDGLPTYNFANVVDDHLMGITHVVRGSEYLSSSPKYNRLYEAFGWDVPIYV
HCPPIMKDEHHKLSKRNGDASFEDLMAKGYLKEAILNYIALLGWNPGGEKEVFSMEELIEAFNYRNINKAPAVFDTKKLK
WMNGEYIRALSLDKFHEMALPYYEEALTRDLDTKKISELLHTRVEVLNEIPEQLDFFNNLLEYSPKMYIHKKMKTTYENS
LKSLEEVLPKLEALENWTFENIKEVCMNLVKELEVKNGVVLWPIRTAVSGKQFTPGGAFEIADILGKEETLERIKIGIDK
LKALQ
>Mature_484_residues
TNKVRTRFAPSPTGYMHVGNLRTALYAYLIAKHDNGDFILRIEDTDQERLVEGALDVIYNTLKITGLSHDEGPDIGGPVG
PYVQSERRNIYIEYAEKLIEKGEAYYCFCSKERLDMLRANSEALKRPFRYDKHCIDLSKEEIDKKIAEGVPYVIRQKNPT
TGSTSFHDEIYGDISVDNSELDDMILIKSDGLPTYNFANVVDDHLMGITHVVRGSEYLSSSPKYNRLYEAFGWDVPIYVH
CPPIMKDEHHKLSKRNGDASFEDLMAKGYLKEAILNYIALLGWNPGGEKEVFSMEELIEAFNYRNINKAPAVFDTKKLKW
MNGEYIRALSLDKFHEMALPYYEEALTRDLDTKKISELLHTRVEVLNEIPEQLDFFNNLLEYSPKMYIHKKMKTTYENSL
KSLEEVLPKLEALENWTFENIKEVCMNLVKELEVKNGVVLWPIRTAVSGKQFTPGGAFEIADILGKEETLERIKIGIDKL
KALQ

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=487, Percent_Identity=34.7022587268994, Blast_Score=268, Evalue=1e-71,
Organism=Escherichia coli, GI1788743, Length=483, Percent_Identity=37.888198757764, Blast_Score=309, Evalue=2e-85,
Organism=Escherichia coli, GI226510925, Length=295, Percent_Identity=33.8983050847458, Blast_Score=157, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI71993394, Length=489, Percent_Identity=36.8098159509202, Blast_Score=265, Evalue=6e-71,
Organism=Saccharomyces cerevisiae, GI6324540, Length=503, Percent_Identity=40.5566600397614, Blast_Score=324, Evalue=2e-89,
Organism=Saccharomyces cerevisiae, GI6321192, Length=240, Percent_Identity=25, Blast_Score=70, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24665321, Length=493, Percent_Identity=36.105476673428, Blast_Score=304, Evalue=9e-83,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 55742; Mature: 55611

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNKVRTRFAPSPTGYMHVGNLRTALYAYLIAKHDNGDFILRIEDTDQERLVEGALDVIY
CCCCCCCCCCCCCCCEEEECHHHHHHHHHHHEEECCCCEEEEEECCCHHHHHHHHHHHHH
NTLKITGLSHDEGPDIGGPVGPYVQSERRNIYIEYAEKLIEKGEAYYCFCSKERLDMLRA
HHHHEEECCCCCCCCCCCCCCCCHHCCCCEEEHHHHHHHHHCCCEEEEEECHHHHHHHHC
NSEALKRPFRYDKHCIDLSKEEIDKKIAEGVPYVIRQKNPTTGSTSFHDEIYGDISVDNS
CHHHHHCCHHCCHHHHCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHCCCEECCCC
ELDDMILIKSDGLPTYNFANVVDDHLMGITHVVRGSEYLSSSPKYNRLYEAFGWDVPIYV
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHCCCCEEEE
HCPPIMKDEHHKLSKRNGDASFEDLMAKGYLKEAILNYIALLGWNPGGEKEVFSMEELIE
ECCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
AFNYRNINKAPAVFDTKKLKWMNGEYIRALSLDKFHEMALPYYEEALTRDLDTKKISELL
HHCCCCCCCCCCCCCCCCEEECCCCEEEEECHHHHHHHHCHHHHHHHHCCCCHHHHHHHH
HTRVEVLNEIPEQLDFFNNLLEYSPKMYIHKKMKTTYENSLKSLEEVLPKLEALENWTFE
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
NIKEVCMNLVKELEVKNGVVLWPIRTAVSGKQFTPGGAFEIADILGKEETLERIKIGIDK
HHHHHHHHHHHHHHHCCCEEEEEEHHHCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHH
LKALQ
HHCCC
>Mature Secondary Structure 
TNKVRTRFAPSPTGYMHVGNLRTALYAYLIAKHDNGDFILRIEDTDQERLVEGALDVIY
CCCCCCCCCCCCCCEEEECHHHHHHHHHHHEEECCCCEEEEEECCCHHHHHHHHHHHHH
NTLKITGLSHDEGPDIGGPVGPYVQSERRNIYIEYAEKLIEKGEAYYCFCSKERLDMLRA
HHHHEEECCCCCCCCCCCCCCCCHHCCCCEEEHHHHHHHHHCCCEEEEEECHHHHHHHHC
NSEALKRPFRYDKHCIDLSKEEIDKKIAEGVPYVIRQKNPTTGSTSFHDEIYGDISVDNS
CHHHHHCCHHCCHHHHCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHCCCEECCCC
ELDDMILIKSDGLPTYNFANVVDDHLMGITHVVRGSEYLSSSPKYNRLYEAFGWDVPIYV
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHCCCCEEEE
HCPPIMKDEHHKLSKRNGDASFEDLMAKGYLKEAILNYIALLGWNPGGEKEVFSMEELIE
ECCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
AFNYRNINKAPAVFDTKKLKWMNGEYIRALSLDKFHEMALPYYEEALTRDLDTKKISELL
HHCCCCCCCCCCCCCCCCEEECCCCEEEEECHHHHHHHHCHHHHHHHHCCCCHHHHHHHH
HTRVEVLNEIPEQLDFFNNLLEYSPKMYIHKKMKTTYENSLKSLEEVLPKLEALENWTFE
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
NIKEVCMNLVKELEVKNGVVLWPIRTAVSGKQFTPGGAFEIADILGKEETLERIKIGIDK
HHHHHHHHHHHHHHHCCCEEEEEEHHHCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHH
LKALQ
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA