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The map label for this gene is vanS [H]

Identifier: 170756558

GI number: 170756558

Start: 340191

End: 341111

Strand: Direct

Name: vanS [H]

Synonym: CLD_0487

Alternate gene names: 170756558

Gene position: 340191-341111 (Clockwise)

Preceding gene: 170757618

Following gene: 170756992

Centisome position: 8.59

GC content: 27.04

Gene sequence:

>921_bases
ATGTTAGAATTTATTATAGCAATTTTTGTGGTTTTTATAGTGTTGATGTGTTTTATATTTATAAAAAGGATGAATAAAAC
TACTGATTATATACAAGAAATATCACAAAATGTAAATATAGTGGCTAATGGAAATATGGATATAGATATACCTATCAGAA
CAGGGGACGAGCTAGGAGCATTAGCGTCAGATGTTAATAAAATGGCATATAATATAGAAGAGTTTATGAGAAAAGAAAGA
CAGTGGGAAAAGCAGAAGAATAATTTGATTACAAATTTATCTCATGATTTAAGAACACCCCTTACATCAGTTGTTGGATT
TTTAGAGTTAATCCAAAAGAAGAAATATAAAAATGATAATGAGTTAAACCATTATTGTGAAGTTTCATTAAATAAAGCAA
AGGAATTAAAAAATTCAGTAGACCAGCTTTTTGAGTTTACAAAGTTAAGTAATGGGGATGTAAAATTAAATATATGTAGT
ATATCTCTACACCAATTAATAGAACAAGCTGCTATTGGATTTATCCCATCTTTTGAAAATAGCGGTATGGAATATAGAGT
ATTTTCAAAGGACCCAGGGTTAATAATAAACGGAGATGCTGCTTTACTTGCTAGGGTATTTGAGAATATAATATCTAATG
CTATTAAATATGGAAGTGAAGGTAAATATTTAGATATACATATAGAAAAAGAGAATAATATGGCTGTAGTTAGTTTTGTA
AATTATGGAGATATAATAGAAGAAAAAGATGTGAAAAATTTATTTCAAAGATTATATAGAATAGAAAAATCTTGTGATAA
AAAAGAAGGGACTGGGCTTGGTCTTGCAATTGCAAAAACAATAGTAGATTTACATAATGGGAATATAGATATTATGAGTT
CTAAAGAAAAAACAGAATTTAAAATAAGATTACCATATTAA

Upstream 100 bases:

>100_bases
AACATTAATAATAGTTTTCTCAGTAATTATGGAGTTGCATATTTTTCATCCAAGACTTTATATGAAGTTTATTAATGCAT
ATAATAAAAATAGTTCATTC

Downstream 100 bases:

>100_bases
GTTCTATGAAATAAAAAAATAGTATTAAGTTAGTTTTTTATTTTAAGGAACTTAAGTAAATAAGGTATTCTATAATAAAT
AAATTAAAAATAAAGGAAAA

Product: sensory transduction histidine kinase

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MLEFIIAIFVVFIVLMCFIFIKRMNKTTDYIQEISQNVNIVANGNMDIDIPIRTGDELGALASDVNKMAYNIEEFMRKER
QWEKQKNNLITNLSHDLRTPLTSVVGFLELIQKKKYKNDNELNHYCEVSLNKAKELKNSVDQLFEFTKLSNGDVKLNICS
ISLHQLIEQAAIGFIPSFENSGMEYRVFSKDPGLIINGDAALLARVFENIISNAIKYGSEGKYLDIHIEKENNMAVVSFV
NYGDIIEEKDVKNLFQRLYRIEKSCDKKEGTGLGLAIAKTIVDLHNGNIDIMSSKEKTEFKIRLPY

Sequences:

>Translated_306_residues
MLEFIIAIFVVFIVLMCFIFIKRMNKTTDYIQEISQNVNIVANGNMDIDIPIRTGDELGALASDVNKMAYNIEEFMRKER
QWEKQKNNLITNLSHDLRTPLTSVVGFLELIQKKKYKNDNELNHYCEVSLNKAKELKNSVDQLFEFTKLSNGDVKLNICS
ISLHQLIEQAAIGFIPSFENSGMEYRVFSKDPGLIINGDAALLARVFENIISNAIKYGSEGKYLDIHIEKENNMAVVSFV
NYGDIIEEKDVKNLFQRLYRIEKSCDKKEGTGLGLAIAKTIVDLHNGNIDIMSSKEKTEFKIRLPY
>Mature_306_residues
MLEFIIAIFVVFIVLMCFIFIKRMNKTTDYIQEISQNVNIVANGNMDIDIPIRTGDELGALASDVNKMAYNIEEFMRKER
QWEKQKNNLITNLSHDLRTPLTSVVGFLELIQKKKYKNDNELNHYCEVSLNKAKELKNSVDQLFEFTKLSNGDVKLNICS
ISLHQLIEQAAIGFIPSFENSGMEYRVFSKDPGLIINGDAALLARVFENIISNAIKYGSEGKYLDIHIEKENNMAVVSFV
NYGDIIEEKDVKNLFQRLYRIEKSCDKKEGTGLGLAIAKTIVDLHNGNIDIMSSKEKTEFKIRLPY

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=262, Percent_Identity=27.4809160305344, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1786783, Length=307, Percent_Identity=26.0586319218241, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI145693157, Length=242, Percent_Identity=24.3801652892562, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1786600, Length=296, Percent_Identity=25.3378378378378, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1789149, Length=335, Percent_Identity=25.9701492537313, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1786912, Length=243, Percent_Identity=27.1604938271605, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788713, Length=226, Percent_Identity=21.6814159292035, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788549, Length=251, Percent_Identity=25.4980079681275, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790551, Length=299, Percent_Identity=23.0769230769231, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1790300, Length=230, Percent_Identity=26.5217391304348, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790436, Length=221, Percent_Identity=24.4343891402715, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1790346, Length=258, Percent_Identity=23.2558139534884, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 34996; Mature: 34996

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEFIIAIFVVFIVLMCFIFIKRMNKTTDYIQEISQNVNIVANGNMDIDIPIRTGDELGA
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEECCCEEEEEEECCCCHHHH
LASDVNKMAYNIEEFMRKERQWEKQKNNLITNLSHDLRTPLTSVVGFLELIQKKKYKNDN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCC
ELNHYCEVSLNKAKELKNSVDQLFEFTKLSNGDVKLNICSISLHQLIEQAAIGFIPSFEN
CCCHHHEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCC
SGMEYRVFSKDPGLIINGDAALLARVFENIISNAIKYGSEGKYLDIHIEKENNMAVVSFV
CCCEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEEE
NYGDIIEEKDVKNLFQRLYRIEKSCDKKEGTGLGLAIAKTIVDLHNGNIDIMSSKEKTEF
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCEE
KIRLPY
EEECCC
>Mature Secondary Structure
MLEFIIAIFVVFIVLMCFIFIKRMNKTTDYIQEISQNVNIVANGNMDIDIPIRTGDELGA
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEECCCEEEEEEECCCCHHHH
LASDVNKMAYNIEEFMRKERQWEKQKNNLITNLSHDLRTPLTSVVGFLELIQKKKYKNDN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCC
ELNHYCEVSLNKAKELKNSVDQLFEFTKLSNGDVKLNICSISLHQLIEQAAIGFIPSFEN
CCCHHHEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCC
SGMEYRVFSKDPGLIINGDAALLARVFENIISNAIKYGSEGKYLDIHIEKENNMAVVSFV
CCCEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEEE
NYGDIIEEKDVKNLFQRLYRIEKSCDKKEGTGLGLAIAKTIVDLHNGNIDIMSSKEKTEF
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCEE
KIRLPY
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]