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The map label for this gene is ptsI

Identifier: 170755729

GI number: 170755729

Start: 3749334

End: 3750953

Strand: Reverse

Name: ptsI

Synonym: CLD_1067

Alternate gene names: 170755729

Gene position: 3750953-3749334 (Counterclockwise)

Preceding gene: 170756976

Following gene: 170755452

Centisome position: 94.76

GC content: 30.19

Gene sequence:

>1620_bases
ATGATGAAAAAAGGTATAGCTGCTTCAAAGGGATATGCTATAGGAAAGATTTTTATTAAGGAAGACATAAATATAGAAGT
TGTAGAAAAGAGTATAGATAACATAGAGGAAGAAAAAGAAAGATTTAAAAGTGCATTAAGTAATACTAGAGATCAACTTG
AAAGAATAAAGGACAAAGCACAAAAAGAAGTAGGAGCGGAAAAAGCAGCAGTTTTTGATAGTCATATTATGTTATTAGAT
GATCCAGAATTTGCTGGAGCTGTAGAAATGAATATAGAATCTAATAAAGTCAATTCAGAAAAAGCACTTCAAGAAGTAAT
TGATATGTATAGTTCTATATTTGCTTCTATGGAAGACGAATACATGAGAGAAAGAGGAGCAGACATAAAGGACGTAGGTA
AGAGAATAATGCTAAATCTTATGGGTAAATCAGTAAATAGTATGGACGATTTGGATAAAGATACTATAATAGTAGCTCAA
GATTTGACTCCATCTGATACTGCTCAATTAGATAAAGAAAAAGTAATAGCTTTTTTAACTAATATAGGTGGAAGGACTTC
TCATAGTGCTATAATGGCTAGAACATTAGAAATACCAGCTATAGTTGGTATGAAAGATATAACTGAATCTGTTAAAAATG
GTGATGTAGTAATAGTTGATGGAATTGAAGGTATAGTAATAATAAATCCAGATAAAGAAACTATAGAAAAGTATGAAGAA
AGTAAAAGAACTTTTCTAAAAGAAAAAGAAGAATTAAAGAAACTTATAAATGTTGAAACCATTACTAGAGAAGGAAAAAG
AGTAGAAGTTTGTGGAAATATAGGAAAGCCCCAAGATGTTCATCAAGTATTAGAAAATGGCGGAGAAGGTGTAGGTCTTT
TTAGAACAGAATTTTTATATATGGATAGAGATAATATGCCATCAGAAGATGAACAGTTTGAATCCTATAAATATGCAGTA
GAAAAAATGGAAGGAAGACCTGTAGTCATAAGAACTTTAGATATAGGTGGAGATAAAAAACTGCCATATTTAGAAATGCC
AGAAGAAATGAACCCTTTCCTAGGATATAGAGCTATAAGATTATGTCTTGATAGAAAAGAATTATTTAAAGTTCAATTAA
GAGCATTGCTTAGAGCATCAGCTTTTGGAAATTTAAAAATAATGTTTCCAATGATATCATCTTTATCTGAATTTAAGGCT
GCTAAAGAATTATTAAAAGAATGCATGAGCGAGTTAAAATCAGAAGGAAAAGAATTTAATGAAAATTTAGAAACAGGTAT
AATGGTAGAAATACCAGCAGCTGCCATATGTGCAGATGAACTTGCTAAACATGTAGATTTCTTTAGTATAGGTACTAATG
ATTTAATACAATATACATTAGCTGCAGATAGAATGAATGAAAAAATATCATATCTTTATGATCCAATGCACCCAGCAGTA
TTAAGATTAATAAAGATGACTATAGATGCAGCACATAAAGAAGGAAAATGGTGTGGAATGTGTGGAGAAATGGCTGGGGA
TGAAAATGCTATAGAAACTCTTGTAGAATATGGATTAGATGAGTATTCTATGAGTGCATCTTCTATTTTAACAGCAAAAA
AAATAATTATGAATTCTTAA

Upstream 100 bases:

>100_bases
AATTTATATTTTTATGGCATGGTTTTTGCTAATTAAATTATTATATGGTTATATTTTAAATAAAAAAAATATATGTATTG
GTTATTAATAAGGAGGAAAA

Downstream 100 bases:

>100_bases
TAAAAAAGCTTCATGTTAACTTTATGTTTTCATGGAGTTTTTTTTTACACAATTGATATTTTTAGATATAATTAGATATA
TATCTATATTTTTGGAGGGG

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MMKKGIAASKGYAIGKIFIKEDINIEVVEKSIDNIEEEKERFKSALSNTRDQLERIKDKAQKEVGAEKAAVFDSHIMLLD
DPEFAGAVEMNIESNKVNSEKALQEVIDMYSSIFASMEDEYMRERGADIKDVGKRIMLNLMGKSVNSMDDLDKDTIIVAQ
DLTPSDTAQLDKEKVIAFLTNIGGRTSHSAIMARTLEIPAIVGMKDITESVKNGDVVIVDGIEGIVIINPDKETIEKYEE
SKRTFLKEKEELKKLINVETITREGKRVEVCGNIGKPQDVHQVLENGGEGVGLFRTEFLYMDRDNMPSEDEQFESYKYAV
EKMEGRPVVIRTLDIGGDKKLPYLEMPEEMNPFLGYRAIRLCLDRKELFKVQLRALLRASAFGNLKIMFPMISSLSEFKA
AKELLKECMSELKSEGKEFNENLETGIMVEIPAAAICADELAKHVDFFSIGTNDLIQYTLAADRMNEKISYLYDPMHPAV
LRLIKMTIDAAHKEGKWCGMCGEMAGDENAIETLVEYGLDEYSMSASSILTAKKIIMNS

Sequences:

>Translated_539_residues
MMKKGIAASKGYAIGKIFIKEDINIEVVEKSIDNIEEEKERFKSALSNTRDQLERIKDKAQKEVGAEKAAVFDSHIMLLD
DPEFAGAVEMNIESNKVNSEKALQEVIDMYSSIFASMEDEYMRERGADIKDVGKRIMLNLMGKSVNSMDDLDKDTIIVAQ
DLTPSDTAQLDKEKVIAFLTNIGGRTSHSAIMARTLEIPAIVGMKDITESVKNGDVVIVDGIEGIVIINPDKETIEKYEE
SKRTFLKEKEELKKLINVETITREGKRVEVCGNIGKPQDVHQVLENGGEGVGLFRTEFLYMDRDNMPSEDEQFESYKYAV
EKMEGRPVVIRTLDIGGDKKLPYLEMPEEMNPFLGYRAIRLCLDRKELFKVQLRALLRASAFGNLKIMFPMISSLSEFKA
AKELLKECMSELKSEGKEFNENLETGIMVEIPAAAICADELAKHVDFFSIGTNDLIQYTLAADRMNEKISYLYDPMHPAV
LRLIKMTIDAAHKEGKWCGMCGEMAGDENAIETLVEYGLDEYSMSASSILTAKKIIMNS
>Mature_539_residues
MMKKGIAASKGYAIGKIFIKEDINIEVVEKSIDNIEEEKERFKSALSNTRDQLERIKDKAQKEVGAEKAAVFDSHIMLLD
DPEFAGAVEMNIESNKVNSEKALQEVIDMYSSIFASMEDEYMRERGADIKDVGKRIMLNLMGKSVNSMDDLDKDTIIVAQ
DLTPSDTAQLDKEKVIAFLTNIGGRTSHSAIMARTLEIPAIVGMKDITESVKNGDVVIVDGIEGIVIINPDKETIEKYEE
SKRTFLKEKEELKKLINVETITREGKRVEVCGNIGKPQDVHQVLENGGEGVGLFRTEFLYMDRDNMPSEDEQFESYKYAV
EKMEGRPVVIRTLDIGGDKKLPYLEMPEEMNPFLGYRAIRLCLDRKELFKVQLRALLRASAFGNLKIMFPMISSLSEFKA
AKELLKECMSELKSEGKEFNENLETGIMVEIPAAAICADELAKHVDFFSIGTNDLIQYTLAADRMNEKISYLYDPMHPAV
LRLIKMTIDAAHKEGKWCGMCGEMAGDENAIETLVEYGLDEYSMSASSILTAKKIIMNS

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=540, Percent_Identity=51.6666666666667, Blast_Score=549, Evalue=1e-157,
Organism=Escherichia coli, GI1788726, Length=531, Percent_Identity=34.4632768361582, Blast_Score=350, Evalue=2e-97,
Organism=Escherichia coli, GI48994992, Length=466, Percent_Identity=37.3390557939914, Blast_Score=337, Evalue=1e-93,
Organism=Escherichia coli, GI1789193, Length=538, Percent_Identity=33.6431226765799, Blast_Score=315, Evalue=3e-87,
Organism=Escherichia coli, GI1787994, Length=433, Percent_Identity=30.2540415704388, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI226510935, Length=184, Percent_Identity=30.9782608695652, Blast_Score=97, Evalue=4e-21,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 60574; Mature: 60574

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKKGIAASKGYAIGKIFIKEDINIEVVEKSIDNIEEEKERFKSALSNTRDQLERIKDKA
CCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QKEVGAEKAAVFDSHIMLLDDPEFAGAVEMNIESNKVNSEKALQEVIDMYSSIFASMEDE
HHHHCCHHHHHHCCEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
YMRERGADIKDVGKRIMLNLMGKSVNSMDDLDKDTIIVAQDLTPSDTAQLDKEKVIAFLT
HHHHHCCCHHHHHHHHHHHHHHCCCCHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
NIGGRTSHSAIMARTLEIPAIVGMKDITESVKNGDVVIVDGIEGIVIINPDKETIEKYEE
HCCCCCCHHHHHHHHHCCCHHCCHHHHHHHHCCCCEEEEECCCEEEEECCCHHHHHHHHH
SKRTFLKEKEELKKLINVETITREGKRVEVCGNIGKPQDVHQVLENGGEGVGLFRTEFLY
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHCCCCCEEEEEEEEEE
MDRDNMPSEDEQFESYKYAVEKMEGRPVVIRTLDIGGDKKLPYLEMPEEMNPFLGYRAIR
ECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCHHCCHHHHHHHHH
LCLDRKELFKVQLRALLRASAFGNLKIMFPMISSLSEFKAAKELLKECMSELKSEGKEFN
HHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ENLETGIMVEIPAAAICADELAKHVDFFSIGTNDLIQYTLAADRMNEKISYLYDPMHPAV
CCCCCCEEEECCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LRLIKMTIDAAHKEGKWCGMCGEMAGDENAIETLVEYGLDEYSMSASSILTAKKIIMNS
HHHHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MMKKGIAASKGYAIGKIFIKEDINIEVVEKSIDNIEEEKERFKSALSNTRDQLERIKDKA
CCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QKEVGAEKAAVFDSHIMLLDDPEFAGAVEMNIESNKVNSEKALQEVIDMYSSIFASMEDE
HHHHCCHHHHHHCCEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
YMRERGADIKDVGKRIMLNLMGKSVNSMDDLDKDTIIVAQDLTPSDTAQLDKEKVIAFLT
HHHHHCCCHHHHHHHHHHHHHHCCCCHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
NIGGRTSHSAIMARTLEIPAIVGMKDITESVKNGDVVIVDGIEGIVIINPDKETIEKYEE
HCCCCCCHHHHHHHHHCCCHHCCHHHHHHHHCCCCEEEEECCCEEEEECCCHHHHHHHHH
SKRTFLKEKEELKKLINVETITREGKRVEVCGNIGKPQDVHQVLENGGEGVGLFRTEFLY
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHCCCCCEEEEEEEEEE
MDRDNMPSEDEQFESYKYAVEKMEGRPVVIRTLDIGGDKKLPYLEMPEEMNPFLGYRAIR
ECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCHHCCHHHHHHHHH
LCLDRKELFKVQLRALLRASAFGNLKIMFPMISSLSEFKAAKELLKECMSELKSEGKEFN
HHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ENLETGIMVEIPAAAICADELAKHVDFFSIGTNDLIQYTLAADRMNEKISYLYDPMHPAV
CCCCCCEEEECCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LRLIKMTIDAAHKEGKWCGMCGEMAGDENAIETLVEYGLDEYSMSASSILTAKKIIMNS
HHHHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]