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The map label for this gene is yflA [H]

Identifier: 170755500

GI number: 170755500

Start: 1225874

End: 1227271

Strand: Reverse

Name: yflA [H]

Synonym: CLD_3460

Alternate gene names: 170755500

Gene position: 1227271-1225874 (Counterclockwise)

Preceding gene: 170756286

Following gene: 170755072

Centisome position: 31.01

GC content: 34.69

Gene sequence:

>1398_bases
ATGCAAACGTTTTTAGACATTATCTCAAAGATCTCTGGTTGGATCTGGGGACCACCTATGATGATTTTACTTGTAGGTGG
CGGAATCATACTAACTTTAACATTAGGTTTTGTTCAATTTAGATACTTACCCTTCATATTAAAGCAAACCTTTGGAAGAA
TATTTGATAAACCTGAAGGTGAAGGTACAATAACACCCTTCCAAGCTGTTAGCTCTGCTCTAGCTTCATCAGTTGGTGCT
GCTAACATCATAGGTGTTCCTGTTGCTATTGCTTTTGGTGGTCCAGGTGCTATATTCTGGATGTGGGTAACAGCAATTAT
AGGACAAGCTACTAAATTCTCTGAAGTAGTATTAGGAATAAAATATAGAGAAAAAAATGAAAAGGGAGAATTTGTTGGAG
GACCTGTTTATTACTTAAAAAAAGGTTTAAAATCTCCTTTTCTCGCTACAATGTGTTCTTTCGCATTTATGTTAGAAATT
ATTCCTTCAATTGCAACACAATCCCTTTCCCTATGTGAATCTGCTGAAACTATAGGAATACCAAAATTAGCAACTGGTAT
TATAGTTACAATTCTTGTAGCTCTAGTTGTATATGGTGGTATTCAAAGAATAGGACAAGTTACTGAAAAATTAGTTCCTT
TGATGGCTTTAGCGTTTATATGTTGCTCTCTTGTAATAGTATTTATTAATATCAAAGAATTACCACACGCTTTTGGATTA
ATATTCAAAGGAGCATTTACTCCTATGGCAGCTGCAGGTGGTTTTGGTGGTTCTGTTTTAGCACAAAGTGTTAGATGGGG
TATCGCAAGAGGTGCTTACTCAAACGAAGCTGGTATGGGAAGTGCTCCTATAGCTCACTCTGCTGCAGTTACAGATCACC
CTGTTCGTCAAGGTTTCTGGGGGGTATTTGAGGTTATAGTTGATACTATATTAATTTGTACTATGACAGCTCTTGTTATT
TTAACTAGTGGAATATGGGAAAAACTCCCTGCTTCTCAAGCAGCTTCTATGCCTGCATTAGCATTCCAATCAGTATTTGG
TAAGAGTGCTGGTGGTGCAATAGTTTCTTTCAGTTTATTACTATTTGTTTTATCAACTATAATAGTTATAGTTTTTTACT
GTGAAAAACAAGCAGAAGCTTTATTTAACCCAACTTTCGGTAAAATTATAAGATTTATTTGTTTAGGTGCTATAATATAT
GGATCCTTTGGGAACTTAGAAAGTTTGTTTGCAATACTTGATTTTCTTTTAGCTCTAGTTGTTATACCAAACATGATTGG
TGTACTTGCTATGAGAAAAGAAGTTAAAGAATTAAAAATAGCATTCTTCTCTGATCCAAAATATTATCCACCAGCAGGGA
AAAAAATGAACAATAAAAATAATGAAAAAGCTTGCTAA

Upstream 100 bases:

>100_bases
TTCTATTGAAAAACATTTTTTTTTATGTTATCGTTTAAATATACATAAAATTCATAATTATAACATAAAACTAACTACTT
ATATTTTAAGGAGGTTAATC

Downstream 100 bases:

>100_bases
TTTGTAATATACACTAAATAAAAAGTCAGGAAATCCTGACTTTTTATTTATATTCTTTTTTATTTATATTCTCTTATTTT
TTACTTAAATAATCTTTTTG

Product: sodium:alanine symporter family protein

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MQTFLDIISKISGWIWGPPMMILLVGGGIILTLTLGFVQFRYLPFILKQTFGRIFDKPEGEGTITPFQAVSSALASSVGA
ANIIGVPVAIAFGGPGAIFWMWVTAIIGQATKFSEVVLGIKYREKNEKGEFVGGPVYYLKKGLKSPFLATMCSFAFMLEI
IPSIATQSLSLCESAETIGIPKLATGIIVTILVALVVYGGIQRIGQVTEKLVPLMALAFICCSLVIVFINIKELPHAFGL
IFKGAFTPMAAAGGFGGSVLAQSVRWGIARGAYSNEAGMGSAPIAHSAAVTDHPVRQGFWGVFEVIVDTILICTMTALVI
LTSGIWEKLPASQAASMPALAFQSVFGKSAGGAIVSFSLLLFVLSTIIVIVFYCEKQAEALFNPTFGKIIRFICLGAIIY
GSFGNLESLFAILDFLLALVVIPNMIGVLAMRKEVKELKIAFFSDPKYYPPAGKKMNNKNNEKAC

Sequences:

>Translated_465_residues
MQTFLDIISKISGWIWGPPMMILLVGGGIILTLTLGFVQFRYLPFILKQTFGRIFDKPEGEGTITPFQAVSSALASSVGA
ANIIGVPVAIAFGGPGAIFWMWVTAIIGQATKFSEVVLGIKYREKNEKGEFVGGPVYYLKKGLKSPFLATMCSFAFMLEI
IPSIATQSLSLCESAETIGIPKLATGIIVTILVALVVYGGIQRIGQVTEKLVPLMALAFICCSLVIVFINIKELPHAFGL
IFKGAFTPMAAAGGFGGSVLAQSVRWGIARGAYSNEAGMGSAPIAHSAAVTDHPVRQGFWGVFEVIVDTILICTMTALVI
LTSGIWEKLPASQAASMPALAFQSVFGKSAGGAIVSFSLLLFVLSTIIVIVFYCEKQAEALFNPTFGKIIRFICLGAIIY
GSFGNLESLFAILDFLLALVVIPNMIGVLAMRKEVKELKIAFFSDPKYYPPAGKKMNNKNNEKAC
>Mature_465_residues
MQTFLDIISKISGWIWGPPMMILLVGGGIILTLTLGFVQFRYLPFILKQTFGRIFDKPEGEGTITPFQAVSSALASSVGA
ANIIGVPVAIAFGGPGAIFWMWVTAIIGQATKFSEVVLGIKYREKNEKGEFVGGPVYYLKKGLKSPFLATMCSFAFMLEI
IPSIATQSLSLCESAETIGIPKLATGIIVTILVALVVYGGIQRIGQVTEKLVPLMALAFICCSLVIVFINIKELPHAFGL
IFKGAFTPMAAAGGFGGSVLAQSVRWGIARGAYSNEAGMGSAPIAHSAAVTDHPVRQGFWGVFEVIVDTILICTMTALVI
LTSGIWEKLPASQAASMPALAFQSVFGKSAGGAIVSFSLLLFVLSTIIVIVFYCEKQAEALFNPTFGKIIRFICLGAIIY
GSFGNLESLFAILDFLLALVVIPNMIGVLAMRKEVKELKIAFFSDPKYYPPAGKKMNNKNNEKAC

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=470, Percent_Identity=32.9787234042553, Blast_Score=226, Evalue=2e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 49819; Mature: 49819

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: PS00873 NA_ALANINE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377 [H]