| Definition | Clostridium botulinum B1 str. Okra, complete genome. |
|---|---|
| Accession | NC_010516 |
| Length | 3,958,233 |
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The map label for this gene is ytbE [H]
Identifier: 170755161
GI number: 170755161
Start: 3664406
End: 3665263
Strand: Reverse
Name: ytbE [H]
Synonym: CLD_1150
Alternate gene names: 170755161
Gene position: 3665263-3664406 (Counterclockwise)
Preceding gene: 170754354
Following gene: 170756398
Centisome position: 92.6
GC content: 38.81
Gene sequence:
>858_bases ATGAAAGAAAATTTTTACTCATTAAGCAATAATTACAAGATTCCGAATATAGGATTCGGTACATTCCGTACCCCTAGTGG AGAGGAAACAGAGCAGTCTGTATTAAATGCAATCAAGGCAGGATATAGGCATATTGACTGCGCTGCTGCTTATGGAAATG AAAAAAGTGTTGGTGAAGCCATACGTAAATCCATACGTAAATCCGGTGTAGCACGCGAAGAATTATTTGTAACAAGTAAG CTATGGAATGATGACAAAGGATATGAAAAGACGTTAGCTGCCTTTAATCGAACCTTAGAGGATTTACAGCTTGATTATTT GGATTTATACCTTATTCACTGGCCTATTGCAAAAGCATCTAAGAATAACTGGCAGGAAGCAAACAGCGAGAGCTGGAGAG CATTCGAGGAACTTTATAAACAGGGTAAAGTTAAAGCAATCGGAGTTAGTAATTTTCTTCCGCACCATTTTGAGCCATTG TTGGCAATCACTAAAATCCAACCGATGGTGAATCAAATTGAATTCCATCCTGGCATGTTGCAGGAGGAAACCGTAGCGTT TTGTAAGCAACGCAATATTTTGGTGGAGGCATGGGCTCCATTTTCAAACGGACAGATTTTCGAGCATCCGGTATTAAAGG AAATTGCGGATCAATACAAAAAATCCGTAGCTCAGCTTAGTCTGCGCTGGATTATTCAAAAAGGAATTATTCCATTGCCA AAGTCTGTTACACCGGAGAGAATTAAGAATAATCTTAAGGTATTTGACTTTGAAATTAGTGCACAAGATGTTGAGAAGAT TGACAGACTAACCGATTGTGGAAGTTCAGGGCTTCATCCAGATAAAGTAGATTTCTAA
Upstream 100 bases:
>100_bases AGACAATTGACGCTGGAAAAGAGAAAATACCTGTTCCTGTAGAAGAAGATCATGTTATGACTAATTTTATACGATAATAA ATCAGAAAGGAAAATAATCT
Downstream 100 bases:
>100_bases GATTATCCAATATCTATCACTATTAAGAAAGTTATTCCTTAAACCGTTGAATAAGTGAATATTAAGAACATGAATAGAGT GCATTTCTTGAACAAGGGAT
Product: aldo/keto reductase family oxidoreductase
Products: 2-keto-L-gulonate; NADP [C]
Alternate protein names: NA
Number of amino acids: Translated: 285; Mature: 285
Protein sequence:
>285_residues MKENFYSLSNNYKIPNIGFGTFRTPSGEETEQSVLNAIKAGYRHIDCAAAYGNEKSVGEAIRKSIRKSGVAREELFVTSK LWNDDKGYEKTLAAFNRTLEDLQLDYLDLYLIHWPIAKASKNNWQEANSESWRAFEELYKQGKVKAIGVSNFLPHHFEPL LAITKIQPMVNQIEFHPGMLQEETVAFCKQRNILVEAWAPFSNGQIFEHPVLKEIADQYKKSVAQLSLRWIIQKGIIPLP KSVTPERIKNNLKVFDFEISAQDVEKIDRLTDCGSSGLHPDKVDF
Sequences:
>Translated_285_residues MKENFYSLSNNYKIPNIGFGTFRTPSGEETEQSVLNAIKAGYRHIDCAAAYGNEKSVGEAIRKSIRKSGVAREELFVTSK LWNDDKGYEKTLAAFNRTLEDLQLDYLDLYLIHWPIAKASKNNWQEANSESWRAFEELYKQGKVKAIGVSNFLPHHFEPL LAITKIQPMVNQIEFHPGMLQEETVAFCKQRNILVEAWAPFSNGQIFEHPVLKEIADQYKKSVAQLSLRWIIQKGIIPLP KSVTPERIKNNLKVFDFEISAQDVEKIDRLTDCGSSGLHPDKVDF >Mature_285_residues MKENFYSLSNNYKIPNIGFGTFRTPSGEETEQSVLNAIKAGYRHIDCAAAYGNEKSVGEAIRKSIRKSGVAREELFVTSK LWNDDKGYEKTLAAFNRTLEDLQLDYLDLYLIHWPIAKASKNNWQEANSESWRAFEELYKQGKVKAIGVSNFLPHHFEPL LAITKIQPMVNQIEFHPGMLQEETVAFCKQRNILVEAWAPFSNGQIFEHPVLKEIADQYKKSVAQLSLRWIIQKGIIPLP KSVTPERIKNNLKVFDFEISAQDVEKIDRLTDCGSSGLHPDKVDF
Specific function: Reduction [C]
COG id: COG0656
COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the aldo/keto reductase family [H]
Homologues:
Organism=Homo sapiens, GI24497577, Length=293, Percent_Identity=39.2491467576792, Blast_Score=221, Evalue=5e-58, Organism=Homo sapiens, GI5174391, Length=293, Percent_Identity=39.2491467576792, Blast_Score=221, Evalue=5e-58, Organism=Homo sapiens, GI223468663, Length=290, Percent_Identity=41.7241379310345, Blast_Score=215, Evalue=4e-56, Organism=Homo sapiens, GI93277124, Length=291, Percent_Identity=37.1134020618557, Blast_Score=192, Evalue=2e-49, Organism=Homo sapiens, GI4502049, Length=286, Percent_Identity=43.3566433566434, Blast_Score=189, Evalue=4e-48, Organism=Homo sapiens, GI291291012, Length=265, Percent_Identity=39.622641509434, Blast_Score=185, Evalue=4e-47, Organism=Homo sapiens, GI310109922, Length=279, Percent_Identity=36.5591397849462, Blast_Score=179, Evalue=3e-45, Organism=Homo sapiens, GI310109920, Length=291, Percent_Identity=35.3951890034364, Blast_Score=178, Evalue=6e-45, Organism=Homo sapiens, GI5453543, Length=291, Percent_Identity=35.3951890034364, Blast_Score=178, Evalue=6e-45, Organism=Homo sapiens, GI5174695, Length=292, Percent_Identity=36.986301369863, Blast_Score=176, Evalue=2e-44, Organism=Homo sapiens, GI45446745, Length=291, Percent_Identity=35.0515463917526, Blast_Score=175, Evalue=4e-44, Organism=Homo sapiens, GI4503285, Length=291, Percent_Identity=35.0515463917526, Blast_Score=175, Evalue=4e-44, Organism=Homo sapiens, GI24497583, Length=297, Percent_Identity=34.006734006734, Blast_Score=172, Evalue=4e-43, Organism=Homo sapiens, GI24497585, Length=291, Percent_Identity=34.3642611683849, Blast_Score=170, Evalue=1e-42, Organism=Homo sapiens, GI300116273, Length=276, Percent_Identity=37.6811594202899, Blast_Score=164, Evalue=8e-41, Organism=Homo sapiens, GI300116271, Length=279, Percent_Identity=33.6917562724014, Blast_Score=146, Evalue=2e-35, Organism=Homo sapiens, GI310109926, Length=113, Percent_Identity=46.0176991150442, Blast_Score=101, Evalue=6e-22, Organism=Homo sapiens, GI207028673, Length=113, Percent_Identity=45.1327433628319, Blast_Score=99, Evalue=4e-21, Organism=Homo sapiens, GI310109924, Length=150, Percent_Identity=32, Blast_Score=88, Evalue=1e-17, Organism=Escherichia coli, GI87082198, Length=277, Percent_Identity=38.9891696750903, Blast_Score=211, Evalue=3e-56, Organism=Escherichia coli, GI1786400, Length=269, Percent_Identity=33.457249070632, Blast_Score=148, Evalue=4e-37, Organism=Escherichia coli, GI1788081, Length=286, Percent_Identity=30.7692307692308, Blast_Score=119, Evalue=2e-28, Organism=Escherichia coli, GI48994888, Length=244, Percent_Identity=25.8196721311475, Blast_Score=68, Evalue=7e-13, Organism=Escherichia coli, GI1788070, Length=310, Percent_Identity=20.9677419354839, Blast_Score=67, Evalue=1e-12, Organism=Caenorhabditis elegans, GI17537077, Length=303, Percent_Identity=37.2937293729373, Blast_Score=197, Evalue=4e-51, Organism=Caenorhabditis elegans, GI71998625, Length=265, Percent_Identity=40.377358490566, Blast_Score=195, Evalue=2e-50, Organism=Caenorhabditis elegans, GI17537075, Length=284, Percent_Identity=37.3239436619718, Blast_Score=193, Evalue=1e-49, Organism=Caenorhabditis elegans, GI17561298, Length=269, Percent_Identity=34.5724907063197, Blast_Score=176, Evalue=1e-44, Organism=Caenorhabditis elegans, GI17552492, Length=264, Percent_Identity=34.0909090909091, Blast_Score=172, Evalue=2e-43, Organism=Caenorhabditis elegans, GI17566692, Length=287, Percent_Identity=34.4947735191638, Blast_Score=171, Evalue=3e-43, Organism=Caenorhabditis elegans, GI17564128, Length=297, Percent_Identity=33.3333333333333, Blast_Score=171, Evalue=5e-43, Organism=Caenorhabditis elegans, GI17538386, Length=273, Percent_Identity=32.967032967033, Blast_Score=164, Evalue=6e-41, Organism=Caenorhabditis elegans, GI17550248, Length=282, Percent_Identity=35.4609929078014, Blast_Score=164, Evalue=7e-41, Organism=Caenorhabditis elegans, GI17537079, Length=292, Percent_Identity=31.1643835616438, Blast_Score=158, Evalue=4e-39, Organism=Caenorhabditis elegans, GI17562292, Length=255, Percent_Identity=29.8039215686275, Blast_Score=141, Evalue=4e-34, Organism=Caenorhabditis elegans, GI17561300, Length=271, Percent_Identity=30.6273062730627, Blast_Score=131, Evalue=4e-31, Organism=Saccharomyces cerevisiae, GI6322556, Length=286, Percent_Identity=37.4125874125874, Blast_Score=199, Evalue=4e-52, Organism=Saccharomyces cerevisiae, GI6324694, Length=291, Percent_Identity=38.1443298969072, Blast_Score=193, Evalue=3e-50, Organism=Saccharomyces cerevisiae, GI6320576, Length=274, Percent_Identity=40.5109489051095, Blast_Score=191, Evalue=8e-50, Organism=Saccharomyces cerevisiae, GI6321896, Length=305, Percent_Identity=35.7377049180328, Blast_Score=174, Evalue=1e-44, Organism=Saccharomyces cerevisiae, GI6319625, Length=299, Percent_Identity=33.1103678929766, Blast_Score=155, Evalue=5e-39, Organism=Saccharomyces cerevisiae, GI6320079, Length=293, Percent_Identity=34.1296928327645, Blast_Score=155, Evalue=9e-39, Organism=Drosophila melanogaster, GI24657054, Length=287, Percent_Identity=40.7665505226481, Blast_Score=216, Evalue=2e-56, Organism=Drosophila melanogaster, GI24662789, Length=293, Percent_Identity=36.8600682593857, Blast_Score=201, Evalue=5e-52, Organism=Drosophila melanogaster, GI24644950, Length=296, Percent_Identity=38.8513513513513, Blast_Score=199, Evalue=1e-51, Organism=Drosophila melanogaster, GI21356425, Length=282, Percent_Identity=38.2978723404255, Blast_Score=198, Evalue=4e-51, Organism=Drosophila melanogaster, GI221378297, Length=316, Percent_Identity=36.0759493670886, Blast_Score=191, Evalue=6e-49, Organism=Drosophila melanogaster, GI24663317, Length=282, Percent_Identity=34.7517730496454, Blast_Score=182, Evalue=2e-46, Organism=Drosophila melanogaster, GI20129731, Length=278, Percent_Identity=34.5323741007194, Blast_Score=176, Evalue=2e-44, Organism=Drosophila melanogaster, GI24662785, Length=270, Percent_Identity=41.4814814814815, Blast_Score=172, Evalue=2e-43, Organism=Drosophila melanogaster, GI24662781, Length=275, Percent_Identity=40.3636363636364, Blast_Score=170, Evalue=8e-43, Organism=Drosophila melanogaster, GI45553081, Length=277, Percent_Identity=31.0469314079422, Blast_Score=152, Evalue=4e-37, Organism=Drosophila melanogaster, GI281366140, Length=244, Percent_Identity=31.1475409836066, Blast_Score=133, Evalue=2e-31,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001395 - InterPro: IPR018170 - InterPro: IPR020471 - InterPro: IPR023210 [H]
Pfam domain/function: PF00248 Aldo_ket_red [H]
EC number: NA
Molecular weight: Translated: 32566; Mature: 32566
Theoretical pI: Translated: 7.20; Mature: 7.20
Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.1 %Cys (Translated Protein) 1.1 %Met (Translated Protein) 2.1 %Cys+Met (Translated Protein) 1.1 %Cys (Mature Protein) 1.1 %Met (Mature Protein) 2.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKENFYSLSNNYKIPNIGFGTFRTPSGEETEQSVLNAIKAGYRHIDCAAAYGNEKSVGEA CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHEEHHHHCCCCHHHHHH IRKSIRKSGVAREELFVTSKLWNDDKGYEKTLAAFNRTLEDLQLDYLDLYLIHWPIAKAS HHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCC KNNWQEANSESWRAFEELYKQGKVKAIGVSNFLPHHFEPLLAITKIQPMVNQIEFHPGML CCCCHHCCCHHHHHHHHHHHCCCEEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCC QEETVAFCKQRNILVEAWAPFSNGQIFEHPVLKEIADQYKKSVAQLSLRWIIQKGIIPLP HHHHHHHHHHCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC KSVTPERIKNNLKVFDFEISAQDVEKIDRLTDCGSSGLHPDKVDF CCCCHHHHHCCCEEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCC >Mature Secondary Structure MKENFYSLSNNYKIPNIGFGTFRTPSGEETEQSVLNAIKAGYRHIDCAAAYGNEKSVGEA CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHEEHHHHCCCCHHHHHH IRKSIRKSGVAREELFVTSKLWNDDKGYEKTLAAFNRTLEDLQLDYLDLYLIHWPIAKAS HHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCC KNNWQEANSESWRAFEELYKQGKVKAIGVSNFLPHHFEPLLAITKIQPMVNQIEFHPGML CCCCHHCCCHHHHHHHHHHHCCCEEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCC QEETVAFCKQRNILVEAWAPFSNGQIFEHPVLKEIADQYKKSVAQLSLRWIIQKGIIPLP HHHHHHHHHHCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC KSVTPERIKNNLKVFDFEISAQDVEKIDRLTDCGSSGLHPDKVDF CCCCHHHHHCCCEEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: 2,5-diketo-D-gluconate; NADPH [C]
Specific reaction: 2,5-diketo-D-gluconate + NADPH = 2-keto-L-gulonate + NADP [C]
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 9387221; 9384377 [H]