Click here to switch to the map view.

The map label for this gene is appA [H]

Identifier: 170755096

GI number: 170755096

Start: 1361919

End: 1363517

Strand: Reverse

Name: appA [H]

Synonym: CLD_3340

Alternate gene names: 170755096

Gene position: 1363517-1361919 (Counterclockwise)

Preceding gene: 170755745

Following gene: 170754630

Centisome position: 34.45

GC content: 29.52

Gene sequence:

>1599_bases
ATGAAAGTGAAGAAACTATTAGCCATGATATTAGTAGCCTCAACTACCTTAGTAGCTACTGCTTGTGGAAATAGCAATAG
TTCTAGCGGATCTACAGCCAAAGAAACTTCCGCCAAAGAAAATATTAAGGATGGAGGAAATTTAGTATTTTCTATCCGTG
GCGAACCAGAAATACTTAATCCGATTTATGCTTATGATAGGGATACTATGACTATGGATAATGCTTTATTTGCACCTTTA
TTTTATATTAATGGAGATAAAATTGATTACACTCTAGCTGAAGAAGTAAAACATTCAGATGATTTTTTAACTTACACAGT
AAAACTAAAAAAAGATTTAAAATGGCATGATGGTAAACCTCTAACTGCTGATGATTTAGTATTTACTATGAAACAAATAA
TGGATGAAAAACAAGATTCACCTTTTAGAAGTGCCTTTGTTATTAATGATAAGCCTGTAGAGGTGAAAAAGATAGATGAT
TTAACTATAGAGTTTAAACTACCAACAGTTCAAATGCCTTTTATGAATTCTTTAGCACAAGTATCTCCTATTCCTAAACA
TGTATTTGAAGGAGAAAAGGACATTAAAAAAAGTGTTAAAAATGAAAAACCTATAGGTTCTGGTGCCTTTAAATTTAAAG
AATCTAGAAAAGGTGAAAGCATAACTTTAGAAAAATTTGATAACTATGTTGGTGGAAAACCTCATCTTGACACAATAACC
TATAGAATAATTGCTGATCCAAATTCATCTAAGGTAGCTCTTGAAAATGGTGAAGTTTCTGCTAATTATATAGATATTAG
TGGTATAAGTAAATTTGAAAAAAATGAAAAATTAAAAGTAGTTGCCTATGATGAAGGTATGGTGGATAATTTAGTCTTAA
ATTGTAAAGCAAAAGGTTTAGATAAAAAAGAAGTTAGACAAGCTATAGCCTATGCTTTAAATAAAGATGATTTAATAAAG
GCTGCTTATGAAAGTGAAAAATATGCCCCTAAGGCCTATTCTCCACTACCTAAGAATGCCTTATATTATACAGAGGATGT
TACTAAATATGACTTAAATAAAGATAAAGCAAAGGAATTACTAAAAAAATCCGGTGCTGAAAATTTAAAACTTAAGCTAG
TTTATAGAAATGATAAAAAGACTTTAGAGAATCAAGCTTTAGTAGTTAAAGAAAATTTAAAAGATATAGGAATAGATGTT
GAATTAAAGGGATTAGAAGCAAATGCCTTTTTTAAGCAAATCGATGATCCTTCTAAAGCTGATTTTGACTTGATATTTAA
TGCCTATTTAATGGGTAACGAGCCTGATGCTTATAAAGAAGTATTTATGGCAAACGGAGCTTTTAACGCTTCTAGATATA
ATAATAAAAAACTAGATGATCTTTGGAATAAAGCTGCCGTAGAAACTAATAAAACAAAGAGAGAAGAAATTTATAAAACT
ATTCAAAAGGAACTTATAGAAGATATGCCTGTATACCCAATTTGCTATTCTAATGCCACAATAGCAGTAAATAAAAATGT
AGGAGGTATAAAAGAAGCAAAAACAGCTCCTATATATATGTTCCAAGACTTATCAAAACTTTATATGATAGAAAAATAA

Upstream 100 bases:

>100_bases
TTATGATTTACTTTTATTATTTAACAATTTTTTATTAAAATGGTATCATTTATTAGTAGAATCAGTAGATTAATTTTTAC
TATTAGAAAGGAGTTTTTTT

Downstream 100 bases:

>100_bases
AAATTTAATAGTTAAAAAGACGGTATTAATTGGATTCCCCTTTTGATACCGTTTATTTTTAAATTAAACAAGTACTTTAA
ACTTTTATAATAGTTATTTA

Product: putative oligopeptide ABC transporter, oligopeptide-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MKVKKLLAMILVASTTLVATACGNSNSSSGSTAKETSAKENIKDGGNLVFSIRGEPEILNPIYAYDRDTMTMDNALFAPL
FYINGDKIDYTLAEEVKHSDDFLTYTVKLKKDLKWHDGKPLTADDLVFTMKQIMDEKQDSPFRSAFVINDKPVEVKKIDD
LTIEFKLPTVQMPFMNSLAQVSPIPKHVFEGEKDIKKSVKNEKPIGSGAFKFKESRKGESITLEKFDNYVGGKPHLDTIT
YRIIADPNSSKVALENGEVSANYIDISGISKFEKNEKLKVVAYDEGMVDNLVLNCKAKGLDKKEVRQAIAYALNKDDLIK
AAYESEKYAPKAYSPLPKNALYYTEDVTKYDLNKDKAKELLKKSGAENLKLKLVYRNDKKTLENQALVVKENLKDIGIDV
ELKGLEANAFFKQIDDPSKADFDLIFNAYLMGNEPDAYKEVFMANGAFNASRYNNKKLDDLWNKAAVETNKTKREEIYKT
IQKELIEDMPVYPICYSNATIAVNKNVGGIKEAKTAPIYMFQDLSKLYMIEK

Sequences:

>Translated_532_residues
MKVKKLLAMILVASTTLVATACGNSNSSSGSTAKETSAKENIKDGGNLVFSIRGEPEILNPIYAYDRDTMTMDNALFAPL
FYINGDKIDYTLAEEVKHSDDFLTYTVKLKKDLKWHDGKPLTADDLVFTMKQIMDEKQDSPFRSAFVINDKPVEVKKIDD
LTIEFKLPTVQMPFMNSLAQVSPIPKHVFEGEKDIKKSVKNEKPIGSGAFKFKESRKGESITLEKFDNYVGGKPHLDTIT
YRIIADPNSSKVALENGEVSANYIDISGISKFEKNEKLKVVAYDEGMVDNLVLNCKAKGLDKKEVRQAIAYALNKDDLIK
AAYESEKYAPKAYSPLPKNALYYTEDVTKYDLNKDKAKELLKKSGAENLKLKLVYRNDKKTLENQALVVKENLKDIGIDV
ELKGLEANAFFKQIDDPSKADFDLIFNAYLMGNEPDAYKEVFMANGAFNASRYNNKKLDDLWNKAAVETNKTKREEIYKT
IQKELIEDMPVYPICYSNATIAVNKNVGGIKEAKTAPIYMFQDLSKLYMIEK
>Mature_532_residues
MKVKKLLAMILVASTTLVATACGNSNSSSGSTAKETSAKENIKDGGNLVFSIRGEPEILNPIYAYDRDTMTMDNALFAPL
FYINGDKIDYTLAEEVKHSDDFLTYTVKLKKDLKWHDGKPLTADDLVFTMKQIMDEKQDSPFRSAFVINDKPVEVKKIDD
LTIEFKLPTVQMPFMNSLAQVSPIPKHVFEGEKDIKKSVKNEKPIGSGAFKFKESRKGESITLEKFDNYVGGKPHLDTIT
YRIIADPNSSKVALENGEVSANYIDISGISKFEKNEKLKVVAYDEGMVDNLVLNCKAKGLDKKEVRQAIAYALNKDDLIK
AAYESEKYAPKAYSPLPKNALYYTEDVTKYDLNKDKAKELLKKSGAENLKLKLVYRNDKKTLENQALVVKENLKDIGIDV
ELKGLEANAFFKQIDDPSKADFDLIFNAYLMGNEPDAYKEVFMANGAFNASRYNNKKLDDLWNKAAVETNKTKREEIYKT
IQKELIEDMPVYPICYSNATIAVNKNVGGIKEAKTAPIYMFQDLSKLYMIEK

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=501, Percent_Identity=27.5449101796407, Blast_Score=156, Evalue=4e-39,
Organism=Escherichia coli, GI1787762, Length=482, Percent_Identity=25.5186721991701, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1789887, Length=486, Percent_Identity=23.4567901234568, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI1787052, Length=479, Percent_Identity=23.7995824634656, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1787551, Length=518, Percent_Identity=23.5521235521236, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1787495, Length=543, Percent_Identity=24.4935543278085, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87081878, Length=526, Percent_Identity=24.7148288973384, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1789397, Length=463, Percent_Identity=23.7580993520518, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87082063, Length=521, Percent_Identity=22.0729366602687, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59947; Mature: 59947

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVKKLLAMILVASTTLVATACGNSNSSSGSTAKETSAKENIKDGGNLVFSIRGEPEILN
CCHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHCHHHCCCCCCCEEEEECCCCCCCC
PIYAYDRDTMTMDNALFAPLFYINGDKIDYTLAEEVKHSDDFLTYTVKLKKDLKWHDGKP
CHHHCCCCCEEECCCCEEEEEEECCCEEEEEHHHHHCCCCCEEEEEEEEEECCCCCCCCC
LTADDLVFTMKQIMDEKQDSPFRSAFVINDKPVEVKKIDDLTIEFKLPTVQMPFMNSLAQ
CCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEECCEEEEEECCEEECHHHHHHHH
VSPIPKHVFEGEKDIKKSVKNEKPIGSGAFKFKESRKGESITLEKFDNYVGGKPHLDTIT
CCCCCHHHHCCHHHHHHHHCCCCCCCCCCEEEHHCCCCCEEEHHHHHCCCCCCCCCCEEE
YRIIADPNSSKVALENGEVSANYIDISGISKFEKNEKLKVVAYDEGMVDNLVLNCKAKGL
EEEEECCCCCEEEEECCCEEEEEEEECCCCHHCCCCCEEEEEECCCCCCCEEEEEECCCC
DKKEVRQAIAYALNKDDLIKAAYESEKYAPKAYSPLPKNALYYTEDVTKYDLNKDKAKEL
CHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCEEECCCHHHHHHH
LKKSGAENLKLKLVYRNDKKTLENQALVVKENLKDIGIDVELKGLEANAFFKQIDDPSKA
HHHCCCCCEEEEEEEECCCHHHHCCEEEEECCHHHCCCEEEEECCCHHHHHHHCCCCCCC
DFDLIFNAYLMGNEPDAYKEVFMANGAFNASRYNNKKLDDLWNKAAVETNKTKREEIYKT
CCEEEEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHH
IQKELIEDMPVYPICYSNATIAVNKNVGGIKEAKTAPIYMFQDLSKLYMIEK
HHHHHHHHCCCCEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MKVKKLLAMILVASTTLVATACGNSNSSSGSTAKETSAKENIKDGGNLVFSIRGEPEILN
CCHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHCHHHCCCCCCCEEEEECCCCCCCC
PIYAYDRDTMTMDNALFAPLFYINGDKIDYTLAEEVKHSDDFLTYTVKLKKDLKWHDGKP
CHHHCCCCCEEECCCCEEEEEEECCCEEEEEHHHHHCCCCCEEEEEEEEEECCCCCCCCC
LTADDLVFTMKQIMDEKQDSPFRSAFVINDKPVEVKKIDDLTIEFKLPTVQMPFMNSLAQ
CCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEECCEEEEEECCEEECHHHHHHHH
VSPIPKHVFEGEKDIKKSVKNEKPIGSGAFKFKESRKGESITLEKFDNYVGGKPHLDTIT
CCCCCHHHHCCHHHHHHHHCCCCCCCCCCEEEHHCCCCCEEEHHHHHCCCCCCCCCCEEE
YRIIADPNSSKVALENGEVSANYIDISGISKFEKNEKLKVVAYDEGMVDNLVLNCKAKGL
EEEEECCCCCEEEEECCCEEEEEEEECCCCHHCCCCCEEEEEECCCCCCCEEEEEECCCC
DKKEVRQAIAYALNKDDLIKAAYESEKYAPKAYSPLPKNALYYTEDVTKYDLNKDKAKEL
CHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCEEECCCHHHHHHH
LKKSGAENLKLKLVYRNDKKTLENQALVVKENLKDIGIDVELKGLEANAFFKQIDDPSKA
HHHCCCCCEEEEEEEECCCHHHHCCEEEEECCHHHCCCEEEEECCCHHHHHHHCCCCCCC
DFDLIFNAYLMGNEPDAYKEVFMANGAFNASRYNNKKLDDLWNKAAVETNKTKREEIYKT
CCEEEEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHH
IQKELIEDMPVYPICYSNATIAVNKNVGGIKEAKTAPIYMFQDLSKLYMIEK
HHHHHHHHCCCCEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]