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The map label for this gene is fumB [H]

Identifier: 170755007

GI number: 170755007

Start: 3754194

End: 3754757

Strand: Reverse

Name: fumB [H]

Synonym: CLD_1064

Alternate gene names: 170755007

Gene position: 3754757-3754194 (Counterclockwise)

Preceding gene: 170756587

Following gene: 170756976

Centisome position: 94.86

GC content: 31.21

Gene sequence:

>564_bases
GTGATTTTAATGAAAATAAAAATAAATACTCCATTAACTGAAGATAAAATCAAAAGCTTAAAAGCAGGGGATATGGTTTT
AATTACAGGTACGATATATACAGCTAGAGATGCGGCACATAAAAGATTAATTGATGCTTTAGAAAAAGGAAGGAATTTAC
CATTTGAAGTGAAAAATTCAATAATATATTATGTTGGACCTACTCCGGCTAAGCCTGGCATGGAAATAGGAGCAGCAGGG
CCAACTACAAGTTATAGGATGGATACATATACACCCAAACTATTGAATTTAGGATTAAAGGGAATGATAGGTAAAGGTAA
AAGGTCTAAAGAGGTTATAGAATCTATAGTAAAAAATAAAGCAGTATATTTTGGGGCAATAGGTGGAGCAGCAGCTTTAA
TATCTAAAAGTATAAAAAAATCAGAAGTTATAGCTTATGAGGACTTAGATTCAGAATCTATCAGAAAGTTAGAAGTAGAA
GATCTGCCCGTTACTGTTATAATAGATTCTAAAGGAAATAATTTATATGAGGATGGATTGGAAGATTATTTGAAAAGTTT
ATAA

Upstream 100 bases:

>100_bases
ATAATATAAAAATTATAGTTTATATTATTTATATATTCTATGTGTAAAATAAATAAATATAACCTCCTTTAATATATTCT
AAAGGAGATATAAAGAGCAG

Downstream 100 bases:

>100_bases
TAATGTAGAAATTTAAACGATAATAATATTAAATATTTACTTTTTAAATGTAGGATAGAGGGGTATATAATGAGAAAGGA
TAATAAATTTAGAAAGGGTT

Product: tartrate/fumarate subfamily Fe-S type hydro-lyase beta subunit

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MILMKIKINTPLTEDKIKSLKAGDMVLITGTIYTARDAAHKRLIDALEKGRNLPFEVKNSIIYYVGPTPAKPGMEIGAAG
PTTSYRMDTYTPKLLNLGLKGMIGKGKRSKEVIESIVKNKAVYFGAIGGAAALISKSIKKSEVIAYEDLDSESIRKLEVE
DLPVTVIIDSKGNNLYEDGLEDYLKSL

Sequences:

>Translated_187_residues
MILMKIKINTPLTEDKIKSLKAGDMVLITGTIYTARDAAHKRLIDALEKGRNLPFEVKNSIIYYVGPTPAKPGMEIGAAG
PTTSYRMDTYTPKLLNLGLKGMIGKGKRSKEVIESIVKNKAVYFGAIGGAAALISKSIKKSEVIAYEDLDSESIRKLEVE
DLPVTVIIDSKGNNLYEDGLEDYLKSL
>Mature_187_residues
MILMKIKINTPLTEDKIKSLKAGDMVLITGTIYTARDAAHKRLIDALEKGRNLPFEVKNSIIYYVGPTPAKPGMEIGAAG
PTTSYRMDTYTPKLLNLGLKGMIGKGKRSKEVIESIVKNKAVYFGAIGGAAALISKSIKKSEVIAYEDLDSESIRKLEVE
DLPVTVIIDSKGNNLYEDGLEDYLKSL

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1838

COG function: function code C; Tartrate dehydratase beta subunit/Fumarate hydratase class I, C-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=179, Percent_Identity=37.9888268156425, Blast_Score=128, Evalue=2e-31,
Organism=Escherichia coli, GI1787897, Length=179, Percent_Identity=35.7541899441341, Blast_Score=124, Evalue=4e-30,
Organism=Escherichia coli, GI1789443, Length=177, Percent_Identity=32.2033898305085, Blast_Score=96, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 20452; Mature: 20452

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILMKIKINTPLTEDKIKSLKAGDMVLITGTIYTARDAAHKRLIDALEKGRNLPFEVKNS
CEEEEEEECCCCCHHHHHHCCCCCEEEEEECEEEHHHHHHHHHHHHHHHCCCCCEEECCC
IIYYVGPTPAKPGMEIGAAGPTTSYRMDTYTPKLLNLGLKGMIGKGKRSKEVIESIVKNK
EEEEECCCCCCCCCEECCCCCCCCEEECCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHCC
AVYFGAIGGAAALISKSIKKSEVIAYEDLDSESIRKLEVEDLPVTVIIDSKGNNLYEDGL
EEEEECCCHHHHHHHHHHHHHHEEEEECCCCHHHHEEEECCCCEEEEEECCCCCCHHHHH
EDYLKSL
HHHHHCC
>Mature Secondary Structure
MILMKIKINTPLTEDKIKSLKAGDMVLITGTIYTARDAAHKRLIDALEKGRNLPFEVKNS
CEEEEEEECCCCCHHHHHHCCCCCEEEEEECEEEHHHHHHHHHHHHHHHCCCCCEEECCC
IIYYVGPTPAKPGMEIGAAGPTTSYRMDTYTPKLLNLGLKGMIGKGKRSKEVIESIVKNK
EEEEECCCCCCCCCEECCCCCCCCEEECCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHCC
AVYFGAIGGAAALISKSIKKSEVIAYEDLDSESIRKLEVEDLPVTVIIDSKGNNLYEDGL
EEEEECCCHHHHHHHHHHHHHHEEEEECCCCHHHHEEEECCCCEEEEEECCCCCCHHHHH
EDYLKSL
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]