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The map label for this gene is eda

Identifier: 170754964

GI number: 170754964

Start: 370832

End: 371479

Strand: Direct

Name: eda

Synonym: CLD_0458

Alternate gene names: 170754964

Gene position: 370832-371479 (Clockwise)

Preceding gene: 170757637

Following gene: 170754933

Centisome position: 9.37

GC content: 31.02

Gene sequence:

>648_bases
GTGATTAAGATGAAGGTTTTAAATGCTCTAAAAAAATGTAAGATAGTAGCAGTTGTTCGTGGAGAAACAAAGGAAGTAGG
TATTAAAATATCAGAAAGCTGTATTAAAGGAAATGTTAAAGCTATAGAAGTTGCATACACAAATAAATTCGCTAATGATA
TAATAGAAGAACTTTCAGAAAAATATGCAAAGGATAATGAAGTTGTAATAGGCGCTGGAACAGTTTTGGATTCAGAAACA
GCAAGAATGGCAATATTAAGAGGAGCAAAATATATAGTTTCTCCATCATTTAGTGAAGAAACAGCAAAGCTTTGTAATAG
ATATAATATTCCATATATACCAGGAGTAATGACTATAACTGAAATAGTTACAGCCTATGAAAGTGGAGTAGACATTGTGA
AGGTATTTCCAGGAAGTGCATTTGGACAAGGTTATATAAAGTCTATTAAAGGACCATTACCATATGCAAATATAATGGTA
ACAGGTGGAGTGAACTTAGATAATATAGACTCATGGGCAAATACAGATGTAGATTTAGTTGGTATTGGTGGAGAATTAAA
TAAACTTGGAGAGTTAGGAAAATTTGATGAAATAGAAGCAATTTGTAATCAATATGTAGATAAATTTAACAAAGCAAGGG
GTATATAA

Upstream 100 bases:

>100_bases
ATTTTCAGAAAGAGCAGTTTCACCAATGAAGGGTGGATATATGGAATAATAATATTTCTGTAAGAGTTAGTACAATAATA
GATTTATGGAGGTTTTTATT

Downstream 100 bases:

>100_bases
CATGAAAGTAGTTGGTTTTGGAGAGATATTACTTAGATTATCTACTAAGAAAGGAATGCTTTTTTTAAATTCTAAGGAAT
TTGATGCTAATTATGGTGGT

Product: 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MIKMKVLNALKKCKIVAVVRGETKEVGIKISESCIKGNVKAIEVAYTNKFANDIIEELSEKYAKDNEVVIGAGTVLDSET
ARMAILRGAKYIVSPSFSEETAKLCNRYNIPYIPGVMTITEIVTAYESGVDIVKVFPGSAFGQGYIKSIKGPLPYANIMV
TGGVNLDNIDSWANTDVDLVGIGGELNKLGELGKFDEIEAICNQYVDKFNKARGI

Sequences:

>Translated_215_residues
MIKMKVLNALKKCKIVAVVRGETKEVGIKISESCIKGNVKAIEVAYTNKFANDIIEELSEKYAKDNEVVIGAGTVLDSET
ARMAILRGAKYIVSPSFSEETAKLCNRYNIPYIPGVMTITEIVTAYESGVDIVKVFPGSAFGQGYIKSIKGPLPYANIMV
TGGVNLDNIDSWANTDVDLVGIGGELNKLGELGKFDEIEAICNQYVDKFNKARGI
>Mature_215_residues
MIKMKVLNALKKCKIVAVVRGETKEVGIKISESCIKGNVKAIEVAYTNKFANDIIEELSEKYAKDNEVVIGAGTVLDSET
ARMAILRGAKYIVSPSFSEETAKLCNRYNIPYIPGVMTITEIVTAYESGVDIVKVFPGSAFGQGYIKSIKGPLPYANIMV
TGGVNLDNIDSWANTDVDLVGIGGELNKLGELGKFDEIEAICNQYVDKFNKARGI

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI48994954, Length=181, Percent_Identity=30.3867403314917, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788156, Length=188, Percent_Identity=26.5957446808511, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 23353; Mature: 23353

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKMKVLNALKKCKIVAVVRGETKEVGIKISESCIKGNVKAIEVAYTNKFANDIIEELSE
CCHHHHHHHHHHCEEEEEEECCCHHHCEEEEHHHHCCCCEEEEEEHHHHHHHHHHHHHHH
KYAKDNEVVIGAGTVLDSETARMAILRGAKYIVSPSFSEETAKLCNRYNIPYIPGVMTIT
HHCCCCCEEEEECCEECCHHHHHHHHHCCCEEECCCCCHHHHHHHHCCCCCCCCHHHHHH
EIVTAYESGVDIVKVFPGSAFGQGYIKSIKGPLPYANIMVTGGVNLDNIDSWANTDVDLV
HHHHHHHCCCCEEEECCCCCCCCHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEE
GIGGELNKLGELGKFDEIEAICNQYVDKFNKARGI
ECCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKMKVLNALKKCKIVAVVRGETKEVGIKISESCIKGNVKAIEVAYTNKFANDIIEELSE
CCHHHHHHHHHHCEEEEEEECCCHHHCEEEEHHHHCCCCEEEEEEHHHHHHHHHHHHHHH
KYAKDNEVVIGAGTVLDSETARMAILRGAKYIVSPSFSEETAKLCNRYNIPYIPGVMTIT
HHCCCCCEEEEECCEECCHHHHHHHHHCCCEEECCCCCHHHHHHHHCCCCCCCCHHHHHH
EIVTAYESGVDIVKVFPGSAFGQGYIKSIKGPLPYANIMVTGGVNLDNIDSWANTDVDLV
HHHHHHHCCCCEEEECCCCCCCCHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEE
GIGGELNKLGELGKFDEIEAICNQYVDKFNKARGI
ECCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377 [H]