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The map label for this gene is licH [H]

Identifier: 170754490

GI number: 170754490

Start: 3654341

End: 3655678

Strand: Reverse

Name: licH [H]

Synonym: CLD_1160

Alternate gene names: 170754490

Gene position: 3655678-3654341 (Counterclockwise)

Preceding gene: 170757365

Following gene: 170755200

Centisome position: 92.36

GC content: 31.17

Gene sequence:

>1338_bases
ATGGATAATGGCATAAAAATAGTAACTATAGGTGGGGGATCAAGCTATACACCAGAATTAGTTGAAGGATTTATAAAGAG
GTATAATGAGTTACCAGTTAGAGAGTTGTGGTTAGTAGATATAGAGGAAGGAAGAGAAAAGTTACAGATTGTAGGAAATT
TGGCTAAAAGGATGGTAAAAAAAGCAGGAATACCAATGGAAATACATTTAACAATAGATAGGAAAAAAGCATTAAAAGAT
GCTGATTTTGTAACAACACAAATAAGAGTAGGGCTTTTAGATGCAAGAATAAAAGATGAAAAAATTCCTTTAAATCATGG
TGTTATAGGGCAAGAAACTAATGGGGCAGGGGGAATGTTTAAAGCATTAAGGACAATCCCTGTAATTCTAGATATAGATA
AGGATATGAGTAAATTGTGCCCTAATGCTTGGATGATAAATTTTACTAATCCAGCAGGTATGGTTACGGAAGCATTATTA
AGATACGGAAAAAATAAAAAAGTTATAGGACTTTGTAATGTTCCTATAGGTATGGAAAAGGCAGCTGCAAAAGTTTTAGA
GGTTGAAAATAAGAGACTAAGAATAGATTTTGTAGGATTGAACCATATGGTGTTCGGAAAAGAAGTATATGTAGATGGAA
TAAAGGTTACAGATAAATTAATTAACTTATTGGCTGATGGAAATATAGGACAAATAGTTAAAAATGTATCGGACTTTACA
TGGAATAAAGATTTTATAAAAGCTTTAGGAATGATGCCATGTCCATATCACAAATATTATTTTCAAACAAAAGATATGCT
AAAAGAAGAACTAGAACAATTTTCTAAAGGTAAAACCAGGGCGGAAATTGTTAAAAAAGTAGAAGAAGAGTTGTTTAAAC
TTTATAAGAATGAAAATTTAGATATTAAACCTCCTGAATTAGAGAAAAGGGGAGGAGCTTATTATAGTGATGCAGCTTGT
AGGCTTATATGTTCAATATATAATGACAAAAAAGATGTTCAACCAGTAAATGTAAGAAATGATGGAGCTATAATAGGCAT
TCCAAATGAATCTGCTATAGAAATATCGTGTATGATAACAAGAGATGGACCTAAGGCAATAGCAGTAGGGGAATTGCCAA
TAGCAATAAATGGACTTATACAACAGATAAAAACCTTTGAGAGATTAGTAGTAGATTCAGCAGTTTTTGGTGATTATTAT
AAGGCGATATTAGCATTGACTGTAAATCCTTTAGTTCAATCGGATAAACTTGCAAAGACATTGGTAGATGAACTTATGAT
AGCTCATAAAGATTATTTACCACAGTTTAAAGAACTTATAGAAGAAAATTTTAAATAA

Upstream 100 bases:

>100_bases
TTATACAACACATGAACAAGTGGATTTTCCAATAAAAAGGTATTGGCATGATGTTTGCTATATATAATAGTGTACATATA
AAACACTGGGGGTGTGTTCT

Downstream 100 bases:

>100_bases
CGATCATTTTGAAATAGATTACTATTATTAATTATTTATAAATATATAAAAAATATGTATACAAAAAGGGGAGGGTTAGA
AATGAAAAAGTTCTTTGACT

Product: glycosy hydrolase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MDNGIKIVTIGGGSSYTPELVEGFIKRYNELPVRELWLVDIEEGREKLQIVGNLAKRMVKKAGIPMEIHLTIDRKKALKD
ADFVTTQIRVGLLDARIKDEKIPLNHGVIGQETNGAGGMFKALRTIPVILDIDKDMSKLCPNAWMINFTNPAGMVTEALL
RYGKNKKVIGLCNVPIGMEKAAAKVLEVENKRLRIDFVGLNHMVFGKEVYVDGIKVTDKLINLLADGNIGQIVKNVSDFT
WNKDFIKALGMMPCPYHKYYFQTKDMLKEELEQFSKGKTRAEIVKKVEEELFKLYKNENLDIKPPELEKRGGAYYSDAAC
RLICSIYNDKKDVQPVNVRNDGAIIGIPNESAIEISCMITRDGPKAIAVGELPIAINGLIQQIKTFERLVVDSAVFGDYY
KAILALTVNPLVQSDKLAKTLVDELMIAHKDYLPQFKELIEENFK

Sequences:

>Translated_445_residues
MDNGIKIVTIGGGSSYTPELVEGFIKRYNELPVRELWLVDIEEGREKLQIVGNLAKRMVKKAGIPMEIHLTIDRKKALKD
ADFVTTQIRVGLLDARIKDEKIPLNHGVIGQETNGAGGMFKALRTIPVILDIDKDMSKLCPNAWMINFTNPAGMVTEALL
RYGKNKKVIGLCNVPIGMEKAAAKVLEVENKRLRIDFVGLNHMVFGKEVYVDGIKVTDKLINLLADGNIGQIVKNVSDFT
WNKDFIKALGMMPCPYHKYYFQTKDMLKEELEQFSKGKTRAEIVKKVEEELFKLYKNENLDIKPPELEKRGGAYYSDAAC
RLICSIYNDKKDVQPVNVRNDGAIIGIPNESAIEISCMITRDGPKAIAVGELPIAINGLIQQIKTFERLVVDSAVFGDYY
KAILALTVNPLVQSDKLAKTLVDELMIAHKDYLPQFKELIEENFK
>Mature_445_residues
MDNGIKIVTIGGGSSYTPELVEGFIKRYNELPVRELWLVDIEEGREKLQIVGNLAKRMVKKAGIPMEIHLTIDRKKALKD
ADFVTTQIRVGLLDARIKDEKIPLNHGVIGQETNGAGGMFKALRTIPVILDIDKDMSKLCPNAWMINFTNPAGMVTEALL
RYGKNKKVIGLCNVPIGMEKAAAKVLEVENKRLRIDFVGLNHMVFGKEVYVDGIKVTDKLINLLADGNIGQIVKNVSDFT
WNKDFIKALGMMPCPYHKYYFQTKDMLKEELEQFSKGKTRAEIVKKVEEELFKLYKNENLDIKPPELEKRGGAYYSDAAC
RLICSIYNDKKDVQPVNVRNDGAIIGIPNESAIEISCMITRDGPKAIAVGELPIAINGLIQQIKTFERLVVDSAVFGDYY
KAILALTVNPLVQSDKLAKTLVDELMIAHKDYLPQFKELIEENFK

Specific function: Hydrolyzes phospho-beta-glucosides (Probable) [H]

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1788029, Length=445, Percent_Identity=52.1348314606742, Blast_Score=472, Evalue=1e-134,
Organism=Escherichia coli, GI1790560, Length=463, Percent_Identity=27.645788336933, Blast_Score=146, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 49993; Mature: 49993

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: PS01324 GLYCOSYL_HYDROL_F4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNGIKIVTIGGGSSYTPELVEGFIKRYNELPVRELWLVDIEEGREKLQIVGNLAKRMVK
CCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHEEEEEHHHHHHHHHHHHHHHHHHHH
KAGIPMEIHLTIDRKKALKDADFVTTQIRVGLLDARIKDEKIPLNHGVIGQETNGAGGMF
HCCCCEEEEEEECHHHHHCCCHHEEHHHEEEHECCEECCCCCCCCCCCCCCCCCCCCHHH
KALRTIPVILDIDKDMSKLCPNAWMINFTNPAGMVTEALLRYGKNKKVIGLCNVPIGMEK
HHHHHCCEEEECCCCHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCHHH
AAAKVLEVENKRLRIDFVGLNHMVFGKEVYVDGIKVTDKLINLLADGNIGQIVKNVSDFT
HHHHHHHHCCCEEEEEEEECCEEEECCEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHCC
WNKDFIKALGMMPCPYHKYYFQTKDMLKEELEQFSKGKTRAEIVKKVEEELFKLYKNENL
CCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCC
DIKPPELEKRGGAYYSDAACRLICSIYNDKKDVQPVNVRNDGAIIGIPNESAIEISCMIT
CCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCEECCCCEEEECCCCCEEEEEEEEE
RDGPKAIAVGELPIAINGLIQQIKTFERLVVDSAVFGDYYKAILALTVNPLVQSDKLAKT
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVDELMIAHKDYLPQFKELIEENFK
HHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDNGIKIVTIGGGSSYTPELVEGFIKRYNELPVRELWLVDIEEGREKLQIVGNLAKRMVK
CCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHEEEEEHHHHHHHHHHHHHHHHHHHH
KAGIPMEIHLTIDRKKALKDADFVTTQIRVGLLDARIKDEKIPLNHGVIGQETNGAGGMF
HCCCCEEEEEEECHHHHHCCCHHEEHHHEEEHECCEECCCCCCCCCCCCCCCCCCCCHHH
KALRTIPVILDIDKDMSKLCPNAWMINFTNPAGMVTEALLRYGKNKKVIGLCNVPIGMEK
HHHHHCCEEEECCCCHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCHHH
AAAKVLEVENKRLRIDFVGLNHMVFGKEVYVDGIKVTDKLINLLADGNIGQIVKNVSDFT
HHHHHHHHCCCEEEEEEEECCEEEECCEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHCC
WNKDFIKALGMMPCPYHKYYFQTKDMLKEELEQFSKGKTRAEIVKKVEEELFKLYKNENL
CCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCC
DIKPPELEKRGGAYYSDAACRLICSIYNDKKDVQPVNVRNDGAIIGIPNESAIEISCMIT
CCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCEECCCCEEEECCCCCEEEEEEEEE
RDGPKAIAVGELPIAINGLIQQIKTFERLVVDSAVFGDYYKAILALTVNPLVQSDKLAKT
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVDELMIAHKDYLPQFKELIEENFK
HHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8990303; 9384377; 8969509 [H]