| Definition | Clostridium botulinum B1 str. Okra, complete genome. |
|---|---|
| Accession | NC_010516 |
| Length | 3,958,233 |
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The map label for this gene is malX [H]
Identifier: 170754412
GI number: 170754412
Start: 365258
End: 366841
Strand: Direct
Name: malX [H]
Synonym: CLD_0462
Alternate gene names: 170754412
Gene position: 365258-366841 (Clockwise)
Preceding gene: 170757240
Following gene: 170754641
Centisome position: 9.23
GC content: 30.3
Gene sequence:
>1584_bases ATGATGAAAGAAAAGATATTAAATCAATTACAAAACTTTTCTAAAGGAATGTTTGTTCCTGTATTGATATTACCAATAGC CGGTATAATAATTGCAATAGGGAACATTTTAACAAATGCAAAACTAGCAGAATACCTTCCATTTTTAAAGAATGGAATGG TATTTGGATTAGGAAAAATGTTATCTGGTTCATTAGTTTCAATTTTAACTAATTTAGGAATTATATTCTGTGTTGGTCTT GCAGTAGGACTTTCTAAGAAAAAGAAAGCAGAAGCAGGATTTACATCACTTTTAGTATTTTTAGTATTTATAAATGCAAT GAACATTTTTTTAGAATTAAATAATAGATTAGCACCACAAGATGCCTTAAGAGGATCAGGCCAAACTATGGTTTTAGGTG TTCAAGTTCTTGATATGGGAGTTTTCTTAGGAATTATTTTAGGTATTGTTGTTGCTTATATACATAATAGATTTTGTGAA AAAGAATTTGATGGAGCTTTTCAAATTTATGGTGGTTCAAGATTAGTATTTATAATATTAATACCTATTACTGTTTTTTT AGCAATAATATTGAGTTATGTTTGGCCTTCAGTACAATATTTAATTTCAAGTCTTGGTAATTTTATAACAGGGGCGGGAA ATTTTGGTATATTTACTTATGGTACTTTAGAGAGATTACTTATTCCTACTGGATTGCATCACTTAGTTTATACTCCATTT TTATATAGTCCTTTAGGTGGAGTGGCTGAAATAGGAGGAAAAGTATTTGAAGGAGCAAGAAATATATACTTTGCAGAAAT GGCTGATCCTAATATAGCAAAATTATCAGCATCAGTAATTTGGGATGCTAGAGGATTGTCAAAAATGTTTGGTTTAATTG GTGCATGTTTGGCTATGTACCATACAGCATTACCAGACAATAAGTCTAAGATAAAGGCAATTTTAATTCCAGCTGCGGTT ACATCAATATTAGCAGGAGTAACAGAGCCAATAGAATTTTCATTTATGTTCGTAGCACCAATATTATTTGTTATACATGC CGTTTTAAGTGGATTGGGAATGGTGGTATTAAATATTCTTGGAGTTACAGCTATAGGTCCAAATGGGTTAATAGACTTCT TATTATACAACATACCTCTTGGAGTTGCTAAAACAGGATGGCCAATGTTTATTTTGATTGGTTTAGGACAATTCGTTGTA TATTATGTTATATTCAGATTTTTAATAGTTAAGTTTAAACTTAAAACTCCAGGAAGAGAAGAAGATGGAAATACAAAGTT ATATACTAAAAAAGATTATAAAGAAAAGTCTAATGCAAATACAAAGTCAGGTGATTCAAGTCTTGCACCAGTTATAGTAG AAGGGCTTGGTGGCAAGGATAATATATTAAAAGTTGATAATTGTTACACTAGATTAAGATTAGTTGTAAAAGATTCTTCA CTAGTTAATGAACAATTACTAAAAAATGAAACAGCAGCAAATGGAGTAATAATTAAAGGAGAAAATGTACAGGTTGTCTA TGGCTTAAAGGTTACAAGTGTAAGAAAGAGCGTAGACGAGTATCTTGGAATAGAATCAAATTAG
Upstream 100 bases:
>100_bases AACACGTTTTTTACATAAGAAAATCTATATACATATAGATATCTAATTATTATAATGTGAAATGTAAATGGTTTCTCAAA AATAAATAATGGGGGTTACA
Downstream 100 bases:
>100_bases AGTAAAAAATTTAGTTAGGAGTGATTATTAATTTAGTCACTCTTATAAAAATATAGTAAATTGTAACTTGAATAAAAATT CCATTAATTATGAAACAGGT
Product: PTS system, glucose/maltose-specfic, IIBC component
Products: NA
Alternate protein names: Maltose and glucose permease IIC component; PTS system maltose- and glucose-specific EIIC component; Maltose- and glucose-specific phosphotransferase enzyme IIB component; PTS system maltose- and glucose-specific EIIB component [H]
Number of amino acids: Translated: 527; Mature: 527
Protein sequence:
>527_residues MMKEKILNQLQNFSKGMFVPVLILPIAGIIIAIGNILTNAKLAEYLPFLKNGMVFGLGKMLSGSLVSILTNLGIIFCVGL AVGLSKKKKAEAGFTSLLVFLVFINAMNIFLELNNRLAPQDALRGSGQTMVLGVQVLDMGVFLGIILGIVVAYIHNRFCE KEFDGAFQIYGGSRLVFIILIPITVFLAIILSYVWPSVQYLISSLGNFITGAGNFGIFTYGTLERLLIPTGLHHLVYTPF LYSPLGGVAEIGGKVFEGARNIYFAEMADPNIAKLSASVIWDARGLSKMFGLIGACLAMYHTALPDNKSKIKAILIPAAV TSILAGVTEPIEFSFMFVAPILFVIHAVLSGLGMVVLNILGVTAIGPNGLIDFLLYNIPLGVAKTGWPMFILIGLGQFVV YYVIFRFLIVKFKLKTPGREEDGNTKLYTKKDYKEKSNANTKSGDSSLAPVIVEGLGGKDNILKVDNCYTRLRLVVKDSS LVNEQLLKNETAANGVIIKGENVQVVYGLKVTSVRKSVDEYLGIESN
Sequences:
>Translated_527_residues MMKEKILNQLQNFSKGMFVPVLILPIAGIIIAIGNILTNAKLAEYLPFLKNGMVFGLGKMLSGSLVSILTNLGIIFCVGL AVGLSKKKKAEAGFTSLLVFLVFINAMNIFLELNNRLAPQDALRGSGQTMVLGVQVLDMGVFLGIILGIVVAYIHNRFCE KEFDGAFQIYGGSRLVFIILIPITVFLAIILSYVWPSVQYLISSLGNFITGAGNFGIFTYGTLERLLIPTGLHHLVYTPF LYSPLGGVAEIGGKVFEGARNIYFAEMADPNIAKLSASVIWDARGLSKMFGLIGACLAMYHTALPDNKSKIKAILIPAAV TSILAGVTEPIEFSFMFVAPILFVIHAVLSGLGMVVLNILGVTAIGPNGLIDFLLYNIPLGVAKTGWPMFILIGLGQFVV YYVIFRFLIVKFKLKTPGREEDGNTKLYTKKDYKEKSNANTKSGDSSLAPVIVEGLGGKDNILKVDNCYTRLRLVVKDSS LVNEQLLKNETAANGVIIKGENVQVVYGLKVTSVRKSVDEYLGIESN >Mature_527_residues MMKEKILNQLQNFSKGMFVPVLILPIAGIIIAIGNILTNAKLAEYLPFLKNGMVFGLGKMLSGSLVSILTNLGIIFCVGL AVGLSKKKKAEAGFTSLLVFLVFINAMNIFLELNNRLAPQDALRGSGQTMVLGVQVLDMGVFLGIILGIVVAYIHNRFCE KEFDGAFQIYGGSRLVFIILIPITVFLAIILSYVWPSVQYLISSLGNFITGAGNFGIFTYGTLERLLIPTGLHHLVYTPF LYSPLGGVAEIGGKVFEGARNIYFAEMADPNIAKLSASVIWDARGLSKMFGLIGACLAMYHTALPDNKSKIKAILIPAAV TSILAGVTEPIEFSFMFVAPILFVIHAVLSGLGMVVLNILGVTAIGPNGLIDFLLYNIPLGVAKTGWPMFILIGLGQFVV YYVIFRFLIVKFKLKTPGREEDGNTKLYTKKDYKEKSNANTKSGDSSLAPVIVEGLGGKDNILKVDNCYTRLRLVVKDSS LVNEQLLKNETAANGVIIKGENVQVVYGLKVTSVRKSVDEYLGIESN
Specific function: MalX encodes a phosphotransferase system enzyme II that can recognize glucose and maltose as substrates even though these sugars may not represent the natural substrates of the system [H]
COG id: COG1263
COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific
Gene ontology:
Cell location: Cell inner membrane; Multi-pass membrane protein [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 PTS EIIC type-1 domain [H]
Homologues:
Organism=Escherichia coli, GI1787908, Length=523, Percent_Identity=38.2409177820268, Blast_Score=326, Evalue=2e-90, Organism=Escherichia coli, GI1787343, Length=528, Percent_Identity=35.0378787878788, Blast_Score=276, Evalue=3e-75, Organism=Escherichia coli, GI1786894, Length=521, Percent_Identity=32.4376199616123, Blast_Score=219, Evalue=4e-58,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR018113 - InterPro: IPR004719 - InterPro: IPR001996 - InterPro: IPR003352 - InterPro: IPR013013 - InterPro: IPR011535 - InterPro: IPR011301 [H]
Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]
EC number: =2.7.1.69 [H]
Molecular weight: Translated: 57216; Mature: 57216
Theoretical pI: Translated: 9.60; Mature: 9.60
Prosite motif: PS00079 MULTICOPPER_OXIDASE1 ; PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.8 %Cys (Translated Protein) 2.7 %Met (Translated Protein) 3.4 %Cys+Met (Translated Protein) 0.8 %Cys (Mature Protein) 2.7 %Met (Mature Protein) 3.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MMKEKILNQLQNFSKGMFVPVLILPIAGIIIAIGNILTNAKLAEYLPFLKNGMVFGLGKM CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEHHHH LSGSLVSILTNLGIIFCVGLAVGLSKKKKAEAGFTSLLVFLVFINAMNIFLELNNRLAPQ HCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH DALRGSGQTMVLGVQVLDMGVFLGIILGIVVAYIHNRFCEKEFDGAFQIYGGSRLVFIIL HHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEHHHH IPITVFLAIILSYVWPSVQYLISSLGNFITGAGNFGIFTYGTLERLLIPTGLHHLVYTPF HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCHHHHHHHHH LYSPLGGVAEIGGKVFEGARNIYFAEMADPNIAKLSASVIWDARGLSKMFGLIGACLAMY HHCCCCCHHHHCHHHHHCCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH HTALPDNKSKIKAILIPAAVTSILAGVTEPIEFSFMFVAPILFVIHAVLSGLGMVVLNIL HHCCCCCCHHEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH GVTAIGPNGLIDFLLYNIPLGVAKTGWPMFILIGLGQFVVYYVIFRFLIVKFKLKTPGRE HHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCC EDGNTKLYTKKDYKEKSNANTKSGDSSLAPVIVEGLGGKDNILKVDNCYTRLRLVVKDSS CCCCCEEEECCCHHHHCCCCCCCCCCCCCHHHEECCCCCCCEEEHHHHHHHHEEEECCHH LVNEQLLKNETAANGVIIKGENVQVVYGLKVTSVRKSVDEYLGIESN HHHHHHHHCCCCCCCEEEECCCEEEEEECCHHHHHHHHHHHCCCCCC >Mature Secondary Structure MMKEKILNQLQNFSKGMFVPVLILPIAGIIIAIGNILTNAKLAEYLPFLKNGMVFGLGKM CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEHHHH LSGSLVSILTNLGIIFCVGLAVGLSKKKKAEAGFTSLLVFLVFINAMNIFLELNNRLAPQ HCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH DALRGSGQTMVLGVQVLDMGVFLGIILGIVVAYIHNRFCEKEFDGAFQIYGGSRLVFIIL HHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEHHHH IPITVFLAIILSYVWPSVQYLISSLGNFITGAGNFGIFTYGTLERLLIPTGLHHLVYTPF HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCHHHHHHHHH LYSPLGGVAEIGGKVFEGARNIYFAEMADPNIAKLSASVIWDARGLSKMFGLIGACLAMY HHCCCCCHHHHCHHHHHCCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH HTALPDNKSKIKAILIPAAVTSILAGVTEPIEFSFMFVAPILFVIHAVLSGLGMVVLNIL HHCCCCCCHHEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH GVTAIGPNGLIDFLLYNIPLGVAKTGWPMFILIGLGQFVVYYVIFRFLIVKFKLKTPGRE HHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCC EDGNTKLYTKKDYKEKSNANTKSGDSSLAPVIVEGLGGKDNILKVDNCYTRLRLVVKDSS CCCCCEEEECCCHHHHCCCCCCCCCCCCCHHHEECCCCCCCEEEHHHHHHHHEEEECCHH LVNEQLLKNETAANGVIIKGENVQVVYGLKVTSVRKSVDEYLGIESN HHHHHHHHCCCCCCCEEEECCCEEEEEECCHHHHHHHHHHHCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 1856179; 9097039; 9278503; 2670898 [H]