Click here to switch to the map view.

The map label for this gene is purH [H]

Identifier: 170751961

GI number: 170751961

Start: 5918338

End: 5919936

Strand: Direct

Name: purH [H]

Synonym: Mrad2831_5593

Alternate gene names: 170751961

Gene position: 5918338-5919936 (Clockwise)

Preceding gene: 170751960

Following gene: 170751962

Centisome position: 97.38

GC content: 72.86

Gene sequence:

>1599_bases
ATGGCGCACGATCAGGTGCGGGTCTCCCGCGCGCTCCTCTCGGTCTCGGACAAGACCGGACTTGTCGACTTCGCCCGGGC
GCTCCACGCCCGCGGCATCGTGCTGGTCTCGACCGGGGGCACCCACCGCGCCCTGGTGGAGGCCGGCCTGCCGGTCACCG
AGGTCGCCGACCTGACGGGCTTCCCGGAGATGATGGACGGTCGGGTCAAGACGCTGCACCCGGCGGTCCACGGCGGCCTG
CTCGCCATCCGCGACAACCCGGAGCATCAGGCCGCGCTCGCCGCCCACGGGATCGGCGCCATCGATCTTCTCGTGGTCAA
CCTCTACCCGTTCGAGGCCACCATCGCGGCCGACCGGTCGGACGCCGACTGCATCGAGAACATCGATATCGGCGGCCCGG
CGATGATCCGCGCGGCGGCCAAGAACCACGCGGACGTTGCCGTCGTCACCGACGTGTCGGACTACGCCGGCGTGCTCGCC
GCCCTCGAGGCGCAGGACGGGTCGCTCGGCGCGGACCTGCGCCGGAGCCTGGCGCAGAAGGCGTTCGCCCGCACCGCGTC
CTACGATGCCGCCATCGCCAACTGGATGGCGGGCCGCTTCGGCGCCGACGCGCCGGCCCGGTTCCGCGCCTTCGGGGGTG
CCCTGGGCCAGGAGCTGCGCTACGGCGAGAACCCCCACCAGTCGGCGGCCTTCTACCGCGCGCCCGGCAAGGTCCGTGCG
GGCGTGGCCACCGCCCGCCAGCTCCAGGGCAAGGAGCTGTCCTACAACAACTTCAACGACACGGACGCCGCCTACGAGTG
CGTGGCCGAGTTCGACCCGGCCCGGACCGCCGCTGTGGCGATCATCAAGCACGCCAACCCCTGCGGCGTCGCCGAGGGGG
CCAGCCTCCTCGAGGCCTATGAGCGGGCTCTGAGCTGCGACCCGACCTCGGCCTTCGGCGGCATCGTCGCCCTGAACCGG
ACGCTCGACGCCGAGGCGGCCCGCCGGATCGTCGAGATCTTCACCGAGGTGATCATCGCCCCCGACGCCACGCCCGAGGC
GGCGGCGATCGTGGCCGGCAAGAAGAACCTGCGCCTGCTGCTGGCCGGTGGCGTGCCCGATCCGCGGGCCGGCGGCGAGA
CCGTGCGGACCCTCGCGGGCGGCCTGCTGGTCCAGACCCGGGACGCGGCGGTCGTCGACGACATGCCGCTCACGGTCGTG
ACCAAGCGCGCGCCGAGCGAGGCCGAGCTGGCCGATCTCCGGTTCGCCTACCGGGTGGCCAAGCACGTGAAGTCGAACGC
CATCGTGTACGCGAAGGCCGGCGCCACGGTCGGTATCGGCGCGGGTCAGATGTCGCGGGTCGATTCCTCGCGCATCGCCG
CCTGGAAGGCCGCCGAGGGGGCGAAGCGTCTCGGGGTCGCGGAGAGCCTCGCGCTCGGTTCGGTCGTGGCATCGGATGCG
TTCTTCCCGTTCGCGGACGGGCTGATGGCCGCCGCCGAGGCGGGCGCCACCGCGGTGATCCAGCCCGGCGGCTCGATGCG
CGATGCGGACGTCATCGCGGCGGCCGATGAGGCCGGGCTCGCCATGGTCCTGACGGGGCACCGCCACTTCCGGCACTGA

Upstream 100 bases:

>100_bases
GCGGCGTGGCGCCCTGACAGGTTCGCGACGGGGGCGGCTGGCCGGGGTCACCGTTTCCATGCTACCGCGCCGGAAAATCT
CGAACTTTCGGCACGAAGCT

Downstream 100 bases:

>100_bases
GCCGGCAGGCCGTTCCCCGGTTCTCCGAGAAGGCGCGCGACCTCCGCGGTGTCCGGAACTGTCCGGCTACCGCGGAGGTC
TCCCTGTTGCGCGCCGTTAA

Product: phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 532; Mature: 531

Protein sequence:

>532_residues
MAHDQVRVSRALLSVSDKTGLVDFARALHARGIVLVSTGGTHRALVEAGLPVTEVADLTGFPEMMDGRVKTLHPAVHGGL
LAIRDNPEHQAALAAHGIGAIDLLVVNLYPFEATIAADRSDADCIENIDIGGPAMIRAAAKNHADVAVVTDVSDYAGVLA
ALEAQDGSLGADLRRSLAQKAFARTASYDAAIANWMAGRFGADAPARFRAFGGALGQELRYGENPHQSAAFYRAPGKVRA
GVATARQLQGKELSYNNFNDTDAAYECVAEFDPARTAAVAIIKHANPCGVAEGASLLEAYERALSCDPTSAFGGIVALNR
TLDAEAARRIVEIFTEVIIAPDATPEAAAIVAGKKNLRLLLAGGVPDPRAGGETVRTLAGGLLVQTRDAAVVDDMPLTVV
TKRAPSEAELADLRFAYRVAKHVKSNAIVYAKAGATVGIGAGQMSRVDSSRIAAWKAAEGAKRLGVAESLALGSVVASDA
FFPFADGLMAAAEAGATAVIQPGGSMRDADVIAAADEAGLAMVLTGHRHFRH

Sequences:

>Translated_532_residues
MAHDQVRVSRALLSVSDKTGLVDFARALHARGIVLVSTGGTHRALVEAGLPVTEVADLTGFPEMMDGRVKTLHPAVHGGL
LAIRDNPEHQAALAAHGIGAIDLLVVNLYPFEATIAADRSDADCIENIDIGGPAMIRAAAKNHADVAVVTDVSDYAGVLA
ALEAQDGSLGADLRRSLAQKAFARTASYDAAIANWMAGRFGADAPARFRAFGGALGQELRYGENPHQSAAFYRAPGKVRA
GVATARQLQGKELSYNNFNDTDAAYECVAEFDPARTAAVAIIKHANPCGVAEGASLLEAYERALSCDPTSAFGGIVALNR
TLDAEAARRIVEIFTEVIIAPDATPEAAAIVAGKKNLRLLLAGGVPDPRAGGETVRTLAGGLLVQTRDAAVVDDMPLTVV
TKRAPSEAELADLRFAYRVAKHVKSNAIVYAKAGATVGIGAGQMSRVDSSRIAAWKAAEGAKRLGVAESLALGSVVASDA
FFPFADGLMAAAEAGATAVIQPGGSMRDADVIAAADEAGLAMVLTGHRHFRH
>Mature_531_residues
AHDQVRVSRALLSVSDKTGLVDFARALHARGIVLVSTGGTHRALVEAGLPVTEVADLTGFPEMMDGRVKTLHPAVHGGLL
AIRDNPEHQAALAAHGIGAIDLLVVNLYPFEATIAADRSDADCIENIDIGGPAMIRAAAKNHADVAVVTDVSDYAGVLAA
LEAQDGSLGADLRRSLAQKAFARTASYDAAIANWMAGRFGADAPARFRAFGGALGQELRYGENPHQSAAFYRAPGKVRAG
VATARQLQGKELSYNNFNDTDAAYECVAEFDPARTAAVAIIKHANPCGVAEGASLLEAYERALSCDPTSAFGGIVALNRT
LDAEAARRIVEIFTEVIIAPDATPEAAAIVAGKKNLRLLLAGGVPDPRAGGETVRTLAGGLLVQTRDAAVVDDMPLTVVT
KRAPSEAELADLRFAYRVAKHVKSNAIVYAKAGATVGIGAGQMSRVDSSRIAAWKAAEGAKRLGVAESLALGSVVASDAF
FPFADGLMAAAEAGATAVIQPGGSMRDADVIAAADEAGLAMVLTGHRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=605, Percent_Identity=36.6942148760331, Blast_Score=290, Evalue=3e-78,
Organism=Escherichia coli, GI1790439, Length=542, Percent_Identity=56.4575645756458, Blast_Score=535, Evalue=1e-153,
Organism=Caenorhabditis elegans, GI71985564, Length=613, Percent_Identity=33.768352365416, Blast_Score=281, Evalue=5e-76,
Organism=Caenorhabditis elegans, GI71985574, Length=318, Percent_Identity=29.2452830188679, Blast_Score=97, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6323056, Length=604, Percent_Identity=32.9470198675497, Blast_Score=274, Evalue=2e-74,
Organism=Saccharomyces cerevisiae, GI6323768, Length=607, Percent_Identity=33.4431630971993, Blast_Score=272, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24649832, Length=602, Percent_Identity=36.2126245847176, Blast_Score=289, Evalue=4e-78,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 55219; Mature: 55088

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHDQVRVSRALLSVSDKTGLVDFARALHARGIVLVSTGGTHRALVEAGLPVTEVADLTG
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCCCHHHHHCCCCHHHHHHHCC
FPEMMDGRVKTLHPAVHGGLLAIRDNPEHQAALAAHGIGAIDLLVVNLYPFEATIAADRS
CHHHHCCCHHEECHHHHCCEEEEECCCCHHHHHHHCCCCEEEEEEEEEECCEEEEECCCC
DADCIENIDIGGPAMIRAAAKNHADVAVVTDVSDYAGVLAALEAQDGSLGADLRRSLAQK
CCHHHHCCCCCCHHHHHHHHCCCCCEEEEECCHHHHHHHHHEECCCCCCCHHHHHHHHHH
AFARTASYDAAIANWMAGRFGADAPARFRAFGGALGQELRYGENPHQSAAFYRAPGKVRA
HHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHH
GVATARQLQGKELSYNNFNDTDAAYECVAEFDPARTAAVAIIKHANPCGVAEGASLLEAY
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCHHHHEEEEEECCCCCCCHHHHHHHHHH
ERALSCDPTSAFGGIVALNRTLDAEAARRIVEIFTEVIIAPDATPEAAAIVAGKKNLRLL
HHHHCCCCCHHCCCEEEEECCCCHHHHHHHHHHHHHHEECCCCCCCCEEEEECCCCEEEE
LAGGVPDPRAGGETVRTLAGGLLVQTRDAAVVDDMPLTVVTKRAPSEAELADLRFAYRVA
EECCCCCCCCCHHHHHHHHCCEEEEECCCEEECCCCEEEEECCCCCCHHHHHHHHHHHHH
KHVKSNAIVYAKAGATVGIGAGQMSRVDSSRIAAWKAAEGAKRLGVAESLALGSVVASDA
HHHCCCEEEEEECCCEEEECCCHHHHCCCHHHHHHHHHCCHHHCCHHHHHHHHHHHHHCC
FFPFADGLMAAAEAGATAVIQPGGSMRDADVIAAADEAGLAMVLTGHRHFRH
CCHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEECCCCCEEEEEECCHHCCC
>Mature Secondary Structure 
AHDQVRVSRALLSVSDKTGLVDFARALHARGIVLVSTGGTHRALVEAGLPVTEVADLTG
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCCCHHHHHCCCCHHHHHHHCC
FPEMMDGRVKTLHPAVHGGLLAIRDNPEHQAALAAHGIGAIDLLVVNLYPFEATIAADRS
CHHHHCCCHHEECHHHHCCEEEEECCCCHHHHHHHCCCCEEEEEEEEEECCEEEEECCCC
DADCIENIDIGGPAMIRAAAKNHADVAVVTDVSDYAGVLAALEAQDGSLGADLRRSLAQK
CCHHHHCCCCCCHHHHHHHHCCCCCEEEEECCHHHHHHHHHEECCCCCCCHHHHHHHHHH
AFARTASYDAAIANWMAGRFGADAPARFRAFGGALGQELRYGENPHQSAAFYRAPGKVRA
HHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHH
GVATARQLQGKELSYNNFNDTDAAYECVAEFDPARTAAVAIIKHANPCGVAEGASLLEAY
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCHHHHEEEEEECCCCCCCHHHHHHHHHH
ERALSCDPTSAFGGIVALNRTLDAEAARRIVEIFTEVIIAPDATPEAAAIVAGKKNLRLL
HHHHCCCCCHHCCCEEEEECCCCHHHHHHHHHHHHHHEECCCCCCCCEEEEECCCCEEEE
LAGGVPDPRAGGETVRTLAGGLLVQTRDAAVVDDMPLTVVTKRAPSEAELADLRFAYRVA
EECCCCCCCCCHHHHHHHHCCEEEEECCCEEECCCCEEEEECCCCCCHHHHHHHHHHHHH
KHVKSNAIVYAKAGATVGIGAGQMSRVDSSRIAAWKAAEGAKRLGVAESLALGSVVASDA
HHHCCCEEEEEECCCEEEECCCHHHHCCCHHHHHHHHHCCHHHCCHHHHHHHHHHHHHCC
FFPFADGLMAAAEAGATAVIQPGGSMRDADVIAAADEAGLAMVLTGHRHFRH
CCHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEECCCCCEEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]