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The map label for this gene is fixL [H]

Identifier: 170751949

GI number: 170751949

Start: 5902423

End: 5902896

Strand: Direct

Name: fixL [H]

Synonym: Mrad2831_5581

Alternate gene names: 170751949

Gene position: 5902423-5902896 (Clockwise)

Preceding gene: 170751946

Following gene: 170751950

Centisome position: 97.11

GC content: 71.31

Gene sequence:

>474_bases
ATGAGGACGACCGGGCCGACCCTGGCCGCGCCCGGTCCCTCCGAACTTGACGGTCTGGTCGAGGCCATCGGCGACGCCGT
GGTGGTGTCCGATCCGGACGGCGCCATCACCGTCTGGAACCCGGCCGCCGAGCGCATCTTCGGCTTCACCGCCGCCGAGG
CGCTGGGGCAGAGCCTCGACCTCATCACGCCCGAGCGGCACCGTCGCCGCCACTGGGACGGCTACGCCAAGACCATGCGC
ACCGGCGTCACCCGGTACGGGGCAGAGACCCTGAGGGTGCCGGCGCTCCACAAGGACGGCCACCAGCTCTCCATCGCCTT
CACGGTGGGGATGGTGCGGGACGCCTCCGACCACGTGACCGGGATCGTCGCCGTGATCCGCGACGAGACCGATCGCTGGG
CCGAGGAGAAGCGCCTGCGCCAGCGCGTGGCCGAACTCGAAGCTTCCTCGGATGCGAACCGCCGGGCACCTTGA

Upstream 100 bases:

>100_bases
CGCCGTTCTCGCGCGTGACGTTCAGGAAGGGCCGCCCCATGACAAGGCGCCGGACTGCCGCGATTCACCTCTCATCCGCG
CCGCCGACCGGGTGGCGTCC

Downstream 100 bases:

>100_bases
TCCCGCCGGACGTGCCGAAGGTCCCGATCACCAAACCGCACAGGGAGAACGCCCAATGAGCAAGCCGCTGGAAGGCATCA
AGATCATCGACTTCACGCAC

Product: putative PAS/PAC sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MRTTGPTLAAPGPSELDGLVEAIGDAVVVSDPDGAITVWNPAAERIFGFTAAEALGQSLDLITPERHRRRHWDGYAKTMR
TGVTRYGAETLRVPALHKDGHQLSIAFTVGMVRDASDHVTGIVAVIRDETDRWAEEKRLRQRVAELEASSDANRRAP

Sequences:

>Translated_157_residues
MRTTGPTLAAPGPSELDGLVEAIGDAVVVSDPDGAITVWNPAAERIFGFTAAEALGQSLDLITPERHRRRHWDGYAKTMR
TGVTRYGAETLRVPALHKDGHQLSIAFTVGMVRDASDHVTGIVAVIRDETDRWAEEKRLRQRVAELEASSDANRRAP
>Mature_157_residues
MRTTGPTLAAPGPSELDGLVEAIGDAVVVSDPDGAITVWNPAAERIFGFTAAEALGQSLDLITPERHRRRHWDGYAKTMR
TGVTRYGAETLRVPALHKDGHQLSIAFTVGMVRDASDHVTGIVAVIRDETDRWAEEKRLRQRVAELEASSDANRRAP

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: COG2202

COG function: function code T; FOG: PAS/PAC domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 17138; Mature: 17138

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS50112 PAS ; PS50113 PAC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTTGPTLAAPGPSELDGLVEAIGDAVVVSDPDGAITVWNPAAERIFGFTAAEALGQSLD
CCCCCCCCCCCCHHHHHHHHHHHCCEEEEECCCCCEEEECHHHHHHHHHHHHHHHCCCCC
LITPERHRRRHWDGYAKTMRTGVTRYGAETLRVPALHKDGHQLSIAFTVGMVRDASDHVT
CCCCHHHHHHCCHHHHHHHHHHHHHHCHHHEECCCCCCCCCEEEEEEEEEEHHCCCHHHH
GIVAVIRDETDRWAEEKRLRQRVAELEASSDANRRAP
HHHHEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRTTGPTLAAPGPSELDGLVEAIGDAVVVSDPDGAITVWNPAAERIFGFTAAEALGQSLD
CCCCCCCCCCCCHHHHHHHHHHHCCEEEEECCCCCEEEECHHHHHHHHHHHHHHHCCCCC
LITPERHRRRHWDGYAKTMRTGVTRYGAETLRVPALHKDGHQLSIAFTVGMVRDASDHVT
CCCCHHHHHHCCHHHHHHHHHHHHHHCHHHEECCCCCCCCCEEEEEEEEEEHHCCCHHHH
GIVAVIRDETDRWAEEKRLRQRVAELEASSDANRRAP
HHHHEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2842062; 11481432; 8432704 [H]