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The map label for this gene is prfC [H]

Identifier: 170751908

GI number: 170751908

Start: 5858996

End: 5860609

Strand: Direct

Name: prfC [H]

Synonym: Mrad2831_5540

Alternate gene names: 170751908

Gene position: 5858996-5860609 (Clockwise)

Preceding gene: 170751905

Following gene: 170751911

Centisome position: 96.4

GC content: 68.65

Gene sequence:

>1614_bases
ATGCTGATGCAGACCAAGACCGAGGCGAGGCCGGCCGATCCGGTGTCGCGGCGGCGCACCTTCGCGATCATCTCCCACCC
CGACGCGGGCAAGACCACGCTCACCGAGAAGCTGCTGCTGTTCGGGGGCGCGATCCAGCTCGCCGGCGAGGTGAAGGCCA
AGCGCAACAGGGTCTCGACCCGCTCCGATTGGATGGGCATCGAGAAGGAGCGCGGCATCTCGGTGGTCACCTCGGTGATG
ACCTTCGAGTACGGCGACTGCGTCTTCAACCTGCTCGACACGCCCGGCCACGAGGACTTCTCGGAGGATACCTACCGGAC
GCTCACGGCGGTCGATTCGGCCGTGATGGTGATCGACGCCGCGAAGGGCATCGAGGCGCGCACGCGGAAACTGTTCGAGG
TCTGCCGCCTGCGCGACATCCCGATCGTCACCTTCATCAACAAGCTCGACCGCGAGGCCCGCGACCCGTTCGAGCTGCTC
GACGAGATCGAGAAGACGCTGGCGCTCGACGTCGCCCCGGTCACGTGGCCGATCGGCACGGGCCGCAACTTCTCCGGGAC
CTACGAGCTCGGCGGCAACCGCGTGCGCCGGCTCGACGCCGCCGAGGATGCCGGGATGGTGGCGGTGTCCGGCCCGGACG
ACCCGCTGTTCGACGAGCTGCTGACCGAGAACGGCGCGGCCGACGCGTGGCGCGAGGAGGTCGAGCTTGCTGAGGGCGGG
CTGAAGCCGTTCGACCTCGACGCGTTCCGCGAGGGCCACCTGACGCCGGTCTTCTTCGGCTCGGCGCTGCGCAATTTCGG
CGTGCGCGACCTGATCGACGGTCTCGCCAAGGTTGCGCCGCCGCCCCGCGGCCAGGACGCGGACAAGCGGCTCGTCGAGC
CGACCGAGCCGCGCATGACCGGCTTCGTGTTCAAGATCCAGGCGAACATGGACCCGAACCACCGGGACCGCATCGCCTTC
ATGCGGGTCTGCTCGGGCAAGCTCAACCGGGGCATGAAGGCCCGGCTGGTGCGCACCGGCAAGCCGATCTCGCTGTCGGC
GCCGCAGTTCTTCTTCGCGCAGGACAGGGCCATCGCCGACGAGGCCTATGCCGGCGACGTGGTCGGCATCCCGAACCACG
GTACCTTGCGCATCGGCGACACGCTCACCGAGGGCGAGGACATCGTGTTCCGCGGCGTGCCGAGCTTCGCGCCGGAGATC
CTGCGCCGGATCAAGCTCACCGACGCCATGAAGGCGAAGAAGCTGCGCGAGGCGCTCCAGCAGATGGGCGAGGAGGGCGT
CGTCCAGGTCTTCGTGCCGCAGGACGGCAGCCAGGCGATCGTCGGCGTGGTCGGCGCTCTGCAGCTCGACGTGCTGAAGG
AGCGCCTCCAGGCCGAGTACGGCCTGCCGGTCGATTACGAGACCTCGCGCTTCTCGGTCTGCCGCTGGGTGTCGTCCGAT
TCGGAGGCCGAGCTCGACAAGTTCATCGACTCGCACGGCTCGGCCATGGCCAAGGACCTCGACGGCGCCCCGGTCTTCAT
GGCGACCACGGCCTTCTCGCTCCGCTACGAGGAGGAGCGCTACCCGGCCGTGCGCTTCACCGACGTGAAGGATTACCAGA
AGCGGGCGGGGTGA

Upstream 100 bases:

>100_bases
CGTCTTTGCCAATATCGGGGCTTGCCGCTACACGATCCCGAGACCGCGCGAGTGCCGCGCCGCGCACCACCACGTTCCGC
CGACCCAGGGCGTTCGCTCC

Downstream 100 bases:

>100_bases
CGCGTTGCGGCGCCGGCTCGTCTTCGCGGGCGCCGCGTAGCGATCCGGGGGATCCCGACCTTCGGTGAGGTCCCGCCGCC
CTGGGTCTCTTCGCGGCCCT

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 537; Mature: 537

Protein sequence:

>537_residues
MLMQTKTEARPADPVSRRRTFAIISHPDAGKTTLTEKLLLFGGAIQLAGEVKAKRNRVSTRSDWMGIEKERGISVVTSVM
TFEYGDCVFNLLDTPGHEDFSEDTYRTLTAVDSAVMVIDAAKGIEARTRKLFEVCRLRDIPIVTFINKLDREARDPFELL
DEIEKTLALDVAPVTWPIGTGRNFSGTYELGGNRVRRLDAAEDAGMVAVSGPDDPLFDELLTENGAADAWREEVELAEGG
LKPFDLDAFREGHLTPVFFGSALRNFGVRDLIDGLAKVAPPPRGQDADKRLVEPTEPRMTGFVFKIQANMDPNHRDRIAF
MRVCSGKLNRGMKARLVRTGKPISLSAPQFFFAQDRAIADEAYAGDVVGIPNHGTLRIGDTLTEGEDIVFRGVPSFAPEI
LRRIKLTDAMKAKKLREALQQMGEEGVVQVFVPQDGSQAIVGVVGALQLDVLKERLQAEYGLPVDYETSRFSVCRWVSSD
SEAELDKFIDSHGSAMAKDLDGAPVFMATTAFSLRYEEERYPAVRFTDVKDYQKRAG

Sequences:

>Translated_537_residues
MLMQTKTEARPADPVSRRRTFAIISHPDAGKTTLTEKLLLFGGAIQLAGEVKAKRNRVSTRSDWMGIEKERGISVVTSVM
TFEYGDCVFNLLDTPGHEDFSEDTYRTLTAVDSAVMVIDAAKGIEARTRKLFEVCRLRDIPIVTFINKLDREARDPFELL
DEIEKTLALDVAPVTWPIGTGRNFSGTYELGGNRVRRLDAAEDAGMVAVSGPDDPLFDELLTENGAADAWREEVELAEGG
LKPFDLDAFREGHLTPVFFGSALRNFGVRDLIDGLAKVAPPPRGQDADKRLVEPTEPRMTGFVFKIQANMDPNHRDRIAF
MRVCSGKLNRGMKARLVRTGKPISLSAPQFFFAQDRAIADEAYAGDVVGIPNHGTLRIGDTLTEGEDIVFRGVPSFAPEI
LRRIKLTDAMKAKKLREALQQMGEEGVVQVFVPQDGSQAIVGVVGALQLDVLKERLQAEYGLPVDYETSRFSVCRWVSSD
SEAELDKFIDSHGSAMAKDLDGAPVFMATTAFSLRYEEERYPAVRFTDVKDYQKRAG
>Mature_537_residues
MLMQTKTEARPADPVSRRRTFAIISHPDAGKTTLTEKLLLFGGAIQLAGEVKAKRNRVSTRSDWMGIEKERGISVVTSVM
TFEYGDCVFNLLDTPGHEDFSEDTYRTLTAVDSAVMVIDAAKGIEARTRKLFEVCRLRDIPIVTFINKLDREARDPFELL
DEIEKTLALDVAPVTWPIGTGRNFSGTYELGGNRVRRLDAAEDAGMVAVSGPDDPLFDELLTENGAADAWREEVELAEGG
LKPFDLDAFREGHLTPVFFGSALRNFGVRDLIDGLAKVAPPPRGQDADKRLVEPTEPRMTGFVFKIQANMDPNHRDRIAF
MRVCSGKLNRGMKARLVRTGKPISLSAPQFFFAQDRAIADEAYAGDVVGIPNHGTLRIGDTLTEGEDIVFRGVPSFAPEI
LRRIKLTDAMKAKKLREALQQMGEEGVVQVFVPQDGSQAIVGVVGALQLDVLKERLQAEYGLPVDYETSRFSVCRWVSSD
SEAELDKFIDSHGSAMAKDLDGAPVFMATTAFSLRYEEERYPAVRFTDVKDYQKRAG

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=483, Percent_Identity=27.3291925465839, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI19923640, Length=503, Percent_Identity=25.844930417495, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI25306283, Length=397, Percent_Identity=27.455919395466, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI25306287, Length=475, Percent_Identity=25.4736842105263, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI157426893, Length=159, Percent_Identity=35.8490566037736, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI94966754, Length=139, Percent_Identity=32.3741007194245, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1790835, Length=521, Percent_Identity=52.3992322456814, Blast_Score=549, Evalue=1e-157,
Organism=Escherichia coli, GI1789738, Length=491, Percent_Identity=27.4949083503055, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1788922, Length=157, Percent_Identity=34.3949044585987, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI48994988, Length=149, Percent_Identity=34.2281879194631, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17533571, Length=491, Percent_Identity=26.8839103869654, Blast_Score=162, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI17556745, Length=170, Percent_Identity=34.7058823529412, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17557151, Length=132, Percent_Identity=34.0909090909091, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71988811, Length=144, Percent_Identity=28.4722222222222, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71988819, Length=144, Percent_Identity=28.4722222222222, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323098, Length=486, Percent_Identity=28.8065843621399, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6322359, Length=545, Percent_Identity=24.2201834862385, Blast_Score=127, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6323320, Length=162, Percent_Identity=29.6296296296296, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324707, Length=156, Percent_Identity=32.6923076923077, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320593, Length=156, Percent_Identity=32.6923076923077, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24582462, Length=482, Percent_Identity=27.3858921161826, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI221458488, Length=505, Percent_Identity=24.950495049505, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI78706572, Length=175, Percent_Identity=34.2857142857143, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=30.718954248366, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=30.718954248366, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=30.718954248366, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI28574573, Length=144, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59491; Mature: 59491

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMQTKTEARPADPVSRRRTFAIISHPDAGKTTLTEKLLLFGGAIQLAGEVKAKRNRVST
CCCCCCCCCCCCCCHHHCEEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
RSDWMGIEKERGISVVTSVMTFEYGDCVFNLLDTPGHEDFSEDTYRTLTAVDSAVMVIDA
HHHCCCCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCEEEEEEC
AKGIEARTRKLFEVCRLRDIPIVTFINKLDREARDPFELLDEIEKTLALDVAPVTWPIGT
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHEECCCEEEECCC
GRNFSGTYELGGNRVRRLDAAEDAGMVAVSGPDDPLFDELLTENGAADAWREEVELAEGG
CCCCCCEEECCCCEEEEECCCCCCCEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHCC
LKPFDLDAFREGHLTPVFFGSALRNFGVRDLIDGLAKVAPPPRGQDADKRLVEPTEPRMT
CCCCCCHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCE
GFVFKIQANMDPNHRDRIAFMRVCSGKLNRGMKARLVRTGKPISLSAPQFFFAQDRAIAD
EEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEECCCCEEECCCHHHHH
EAYAGDVVGIPNHGTLRIGDTLTEGEDIVFRGVPSFAPEILRRIKLTDAMKAKKLREALQ
HHCCCCEEECCCCCEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QMGEEGVVQVFVPQDGSQAIVGVVGALQLDVLKERLQAEYGLPVDYETSRFSVCRWVSSD
HCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHEEECCCC
SEAELDKFIDSHGSAMAKDLDGAPVFMATTAFSLRYEEERYPAVRFTDVKDYQKRAG
CHHHHHHHHHHCCCHHHHCCCCCCEEEEEEEEEEEECCCCCCEEEEECHHHHHHHCC
>Mature Secondary Structure
MLMQTKTEARPADPVSRRRTFAIISHPDAGKTTLTEKLLLFGGAIQLAGEVKAKRNRVST
CCCCCCCCCCCCCCHHHCEEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
RSDWMGIEKERGISVVTSVMTFEYGDCVFNLLDTPGHEDFSEDTYRTLTAVDSAVMVIDA
HHHCCCCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCEEEEEEC
AKGIEARTRKLFEVCRLRDIPIVTFINKLDREARDPFELLDEIEKTLALDVAPVTWPIGT
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHEECCCEEEECCC
GRNFSGTYELGGNRVRRLDAAEDAGMVAVSGPDDPLFDELLTENGAADAWREEVELAEGG
CCCCCCEEECCCCEEEEECCCCCCCEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHCC
LKPFDLDAFREGHLTPVFFGSALRNFGVRDLIDGLAKVAPPPRGQDADKRLVEPTEPRMT
CCCCCCHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCE
GFVFKIQANMDPNHRDRIAFMRVCSGKLNRGMKARLVRTGKPISLSAPQFFFAQDRAIAD
EEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEECCCCEEECCCHHHHH
EAYAGDVVGIPNHGTLRIGDTLTEGEDIVFRGVPSFAPEILRRIKLTDAMKAKKLREALQ
HHCCCCEEECCCCCEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QMGEEGVVQVFVPQDGSQAIVGVVGALQLDVLKERLQAEYGLPVDYETSRFSVCRWVSSD
HCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHEEECCCC
SEAELDKFIDSHGSAMAKDLDGAPVFMATTAFSLRYEEERYPAVRFTDVKDYQKRAG
CHHHHHHHHHHCCCHHHHCCCCCCEEEEEEEEEEEECCCCCCEEEEECHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA