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The map label for this gene is 170751755

Identifier: 170751755

GI number: 170751755

Start: 5693578

End: 5694945

Strand: Direct

Name: 170751755

Synonym: Mrad2831_5385

Alternate gene names: NA

Gene position: 5693578-5694945 (Clockwise)

Preceding gene: 170751754

Following gene: 170751756

Centisome position: 93.68

GC content: 72.44

Gene sequence:

>1368_bases
ATGGCGCGCGACAGCAACGACACCACGCCGCTCACCACGCGCGCCGAACTGATCGAATGGTTCGCGGCGGGCGAGAAGCC
GCGCGCGCGCTTCGCGATCGGCACCGAGCACGAGAAGATCCCGTTCTACCGCGAGACCCGGACGCCGGTTCCCTACGAGG
GCGACCGCGGCATCCGGGCGCTCCTGGAGGGGCTCGCCCGCGAGACCGGCTGGGAGCCGATCCTCGATCTCGGCAACATC
ATCGGCCTGTCGGCGGAGGCGGGCGGCGCGATCTCGATCGAGCCCGGCGGCCAGTTCGAGCTGTCCGGCGCGGCGCTCCC
CGACGTGCACGCCACGGCGGCGGAGCTCGCCACGCACCTCGCCGCCACGAAGCGGGTCGCCGATCCCCTCGGCATCGGCT
TCCTGACGCTGGGCATGAGCCCGCGATGGCGGCGCGACGAGACCCCGGTCATGCCCAAGAGCCGCTACCGGATCATGGCC
GGCTACATGCCCAAGGTCGGGTCCCTCGGCCTCGACATGATGCTGCGCACCGCCACCGTGCAGGTGAATCTCGATTTCGC
CTCCGAGGCCGACATGGTGCGCAAGATGCGCGCGTCCCTGGCGCTGCAGCCGGTCGCGACCGCGCTCTTCGCCAACTCGC
CCTTCACCGACGGCCGGCCGAACGGCTTCCTGTCGCGCCGGTCCGAGATCTGGCGCGACACGGATGCCCACCGCACCGGA
ATGCTGCCCTTCGCCTTCGACGCGGGCTTCGGTTACGAGGCCTATGTCGACTGGCTGCTCGACATGCCGATGTACTTCGT
CAAGCGGGGCGACACCTACCACGACGTGTCGGGCGCCTCGTTCCGCGACCTGATGGCCGGCAAGCTCCCGCAACTGCCCG
GCGAGTTCGCCACGGTCTCGGACTGGGCCAACCACGCCTCAACGGCCTTCCCGGAGGTGCGGCTCAAGCGCTTCCTCGAG
ATGCGCGGCGCCGATGTCGGCGATCCCGGCATGATCGCCGCCCAGGCGGCCTTCTGGGCGGGGCTCCTCTACGACGAGTC
GGCCCTGGACGCGGCCCTCGACCTCGTGAAGGGGTGGAGCCCCGCCAACCGCGAGGCCGCGCGGGCCACGGCGCCGCGCC
TGGGGCTCGCGACCCCGGTGGCCAACACCACGCTCGGGGCCGTCGCCGCCGAGGCCCTGGCGATCGCCCGGGCGGGCCTC
CGGGCGCGGGGCCGCCGCGACGCCGAGGGTCGGGACGAGGTGATCTACCTGCAGCCGCTCGAGGCCATCGTCGCGGCCGG
GCGCACCCGGGCCGAGGATCTCCTCGCCGACTACGAGGGACGCTGGGGCGCCTGCGTGCGTCCGGCCTTCACCGAGTGCG
TCTTCTAG

Upstream 100 bases:

>100_bases
GGATCCCGCTCGCGAGAGCGGGTTCGTACCGGGTGGATCCGCCGGAGCAGTCCGGCGCGCCCGTTCCCAGGCACAGGTCA
GACGCACGGGCAGGCAGCGC

Downstream 100 bases:

>100_bases
CGACCGCCTGAGCGGCGCTCGTGGCCCGCGCGAATCGACCTTGGAACGTGCTAATGGAACGTTCCGACAGGGCGCCGCGC
TCGGGGAGAGGACGCATCAT

Product: glutamate--cysteine ligase

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Gamma-Glutamylcysteine Synthetase; Glutamate/Cysteine Ligase Family Protein; Glutamate-Cysteine Ligase GshA; Glutamylcysteine Synthetase; Glutamate-Cysteine Ligase Family 2 Protein; Glutamate-Cysteine Ligase Protein; Glutathione Synthetase; Glutamate-Cysteine Ligase Gsha; Glutamate-Cysteine Ligase Protein; Glutamate-Cysteine Ligase Family Protein; Glutamate Cystein Ligase Protein; Glutamate-Cysteine Ligase GSHA

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MARDSNDTTPLTTRAELIEWFAAGEKPRARFAIGTEHEKIPFYRETRTPVPYEGDRGIRALLEGLARETGWEPILDLGNI
IGLSAEAGGAISIEPGGQFELSGAALPDVHATAAELATHLAATKRVADPLGIGFLTLGMSPRWRRDETPVMPKSRYRIMA
GYMPKVGSLGLDMMLRTATVQVNLDFASEADMVRKMRASLALQPVATALFANSPFTDGRPNGFLSRRSEIWRDTDAHRTG
MLPFAFDAGFGYEAYVDWLLDMPMYFVKRGDTYHDVSGASFRDLMAGKLPQLPGEFATVSDWANHASTAFPEVRLKRFLE
MRGADVGDPGMIAAQAAFWAGLLYDESALDAALDLVKGWSPANREAARATAPRLGLATPVANTTLGAVAAEALAIARAGL
RARGRRDAEGRDEVIYLQPLEAIVAAGRTRAEDLLADYEGRWGACVRPAFTECVF

Sequences:

>Translated_455_residues
MARDSNDTTPLTTRAELIEWFAAGEKPRARFAIGTEHEKIPFYRETRTPVPYEGDRGIRALLEGLARETGWEPILDLGNI
IGLSAEAGGAISIEPGGQFELSGAALPDVHATAAELATHLAATKRVADPLGIGFLTLGMSPRWRRDETPVMPKSRYRIMA
GYMPKVGSLGLDMMLRTATVQVNLDFASEADMVRKMRASLALQPVATALFANSPFTDGRPNGFLSRRSEIWRDTDAHRTG
MLPFAFDAGFGYEAYVDWLLDMPMYFVKRGDTYHDVSGASFRDLMAGKLPQLPGEFATVSDWANHASTAFPEVRLKRFLE
MRGADVGDPGMIAAQAAFWAGLLYDESALDAALDLVKGWSPANREAARATAPRLGLATPVANTTLGAVAAEALAIARAGL
RARGRRDAEGRDEVIYLQPLEAIVAAGRTRAEDLLADYEGRWGACVRPAFTECVF
>Mature_454_residues
ARDSNDTTPLTTRAELIEWFAAGEKPRARFAIGTEHEKIPFYRETRTPVPYEGDRGIRALLEGLARETGWEPILDLGNII
GLSAEAGGAISIEPGGQFELSGAALPDVHATAAELATHLAATKRVADPLGIGFLTLGMSPRWRRDETPVMPKSRYRIMAG
YMPKVGSLGLDMMLRTATVQVNLDFASEADMVRKMRASLALQPVATALFANSPFTDGRPNGFLSRRSEIWRDTDAHRTGM
LPFAFDAGFGYEAYVDWLLDMPMYFVKRGDTYHDVSGASFRDLMAGKLPQLPGEFATVSDWANHASTAFPEVRLKRFLEM
RGADVGDPGMIAAQAAFWAGLLYDESALDAALDLVKGWSPANREAARATAPRLGLATPVANTTLGAVAAEALAIARAGLR
ARGRRDAEGRDEVIYLQPLEAIVAAGRTRAEDLLADYEGRWGACVRPAFTECVF

Specific function: Unknown

COG id: COG3572

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 49498; Mature: 49367

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARDSNDTTPLTTRAELIEWFAAGEKPRARFAIGTEHEKIPFYRETRTPVPYEGDRGIRA
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEECCCCCCCCCCCHHHHH
LLEGLARETGWEPILDLGNIIGLSAEAGGAISIEPGGQFELSGAALPDVHATAAELATHL
HHHHHHHHCCCCHHHHHHHHEEECCCCCCEEEECCCCCEEECCCCCCCHHHHHHHHHHHH
AATKRVADPLGIGFLTLGMSPRWRRDETPVMPKSRYRIMAGYMPKVGSLGLDMMLRTATV
HHHHHHCCHHCCCEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHEEEEEEE
QVNLDFASEADMVRKMRASLALQPVATALFANSPFTDGRPNGFLSRRSEIWRDTDAHRTG
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCC
MLPFAFDAGFGYEAYVDWLLDMPMYFVKRGDTYHDVSGASFRDLMAGKLPQLPGEFATVS
CCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHH
DWANHASTAFPEVRLKRFLEMRGADVGDPGMIAAQAAFWAGLLYDESALDAALDLVKGWS
HHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCCC
PANREAARATAPRLGLATPVANTTLGAVAAEALAIARAGLRARGRRDAEGRDEVIYLQPL
CCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCH
EAIVAAGRTRAEDLLADYEGRWGACVRPAFTECVF
HHHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHCC
>Mature Secondary Structure 
ARDSNDTTPLTTRAELIEWFAAGEKPRARFAIGTEHEKIPFYRETRTPVPYEGDRGIRA
CCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEECCCCCCCCCCCHHHHH
LLEGLARETGWEPILDLGNIIGLSAEAGGAISIEPGGQFELSGAALPDVHATAAELATHL
HHHHHHHHCCCCHHHHHHHHEEECCCCCCEEEECCCCCEEECCCCCCCHHHHHHHHHHHH
AATKRVADPLGIGFLTLGMSPRWRRDETPVMPKSRYRIMAGYMPKVGSLGLDMMLRTATV
HHHHHHCCHHCCCEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHEEEEEEE
QVNLDFASEADMVRKMRASLALQPVATALFANSPFTDGRPNGFLSRRSEIWRDTDAHRTG
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCC
MLPFAFDAGFGYEAYVDWLLDMPMYFVKRGDTYHDVSGASFRDLMAGKLPQLPGEFATVS
CCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHH
DWANHASTAFPEVRLKRFLEMRGADVGDPGMIAAQAAFWAGLLYDESALDAALDLVKGWS
HHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCCC
PANREAARATAPRLGLATPVANTTLGAVAAEALAIARAGLRARGRRDAEGRDEVIYLQPL
CCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCH
EAIVAAGRTRAEDLLADYEGRWGACVRPAFTECVF
HHHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA