Click here to switch to the map view.

The map label for this gene is rsmE [H]

Identifier: 170751754

GI number: 170751754

Start: 5692532

End: 5693284

Strand: Direct

Name: rsmE [H]

Synonym: Mrad2831_5384

Alternate gene names: 170751754

Gene position: 5692532-5693284 (Clockwise)

Preceding gene: 170751751

Following gene: 170751755

Centisome position: 93.66

GC content: 75.3

Gene sequence:

>753_bases
ATGCCGGCCTACGACTTCACCGCTCCGCGCCTCCACGTCGCGGCCGATCTCGCCGCGGGGGTGATTCTGCCCCTCGACCG
AGCCCAGGCCAACTACCTGCTCAACGTGCTGCGCCGCGGGCCGGACGATCCGGTCCTGGTCTTCAACGGCCGCCACGGCG
AGTGGCGCGCGCACCTGGTCCAGACCGGGCGCAAGGGCGCCGACCTCCACGTCGCCGCGCAGACGCGCCCGCAGACCGAG
CGCGCCGACCTGCACTACCTGTTCGCCCCGCTGAAGAGCGCGCGGCTCGACTACCTCGCGCAGAAGGCGGTGGAGATGGG
GGCCGGGACGATCCGTCCGGTTATCACCCGGTTCACGCAGGGCGACCGCGTCAACATCGACCGGCTGCGAGCCAACGCCG
TCGAGGCCGCCGAGCAGTGCGGGATCCTGGCGATTCCCGAATGTCCCGAGCCCGAGCGCCTCCCGGCCGCGCTCGCGGGC
CTCGCGCCCGGGCGGCTCCTCGTGTTCTGCGACGAGGACGCGCCCGTGCGCGACCCCGTCGCGGCCCTGCGGGCGGCCGG
CGACCCGCTGGCGCCGCCCCCGCTCGCCGTGCTGGTCGGCCCCGAGGGCGGCTTCTCGGAGGAGGAGCGGGCGCTGATCC
TGGCTCGGCCGAACACCGTGGCGCTCTCCCTCGGGCCGCGCATCCTGCGGGCCGACACCGCCGCCGTCGCCGTGCTGACC
CTGGTGCAGGCGGTGCTCGGCGACGCGCGCTGA

Upstream 100 bases:

>100_bases
TGGACTGGCTGCGGAAAACCGGGGCTCGTAACCATTCGGCAACACTTTCGGGAACGCATCCCGCGTTGACAGGCCCGCCG
ACCCGCGCGACCACGCCGCC

Downstream 100 bases:

>100_bases
CGGCGGGCCGATCCTTCCATGTGTGCCCGCGCACATGGTAGGATCGCGCCGGGCGATTGTTTCGCTGGATCGGACGTGAA
GCGGCGCGCCGGTGGAGACC

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MPAYDFTAPRLHVAADLAAGVILPLDRAQANYLLNVLRRGPDDPVLVFNGRHGEWRAHLVQTGRKGADLHVAAQTRPQTE
RADLHYLFAPLKSARLDYLAQKAVEMGAGTIRPVITRFTQGDRVNIDRLRANAVEAAEQCGILAIPECPEPERLPAALAG
LAPGRLLVFCDEDAPVRDPVAALRAAGDPLAPPPLAVLVGPEGGFSEEERALILARPNTVALSLGPRILRADTAAVAVLT
LVQAVLGDAR

Sequences:

>Translated_250_residues
MPAYDFTAPRLHVAADLAAGVILPLDRAQANYLLNVLRRGPDDPVLVFNGRHGEWRAHLVQTGRKGADLHVAAQTRPQTE
RADLHYLFAPLKSARLDYLAQKAVEMGAGTIRPVITRFTQGDRVNIDRLRANAVEAAEQCGILAIPECPEPERLPAALAG
LAPGRLLVFCDEDAPVRDPVAALRAAGDPLAPPPLAVLVGPEGGFSEEERALILARPNTVALSLGPRILRADTAAVAVLT
LVQAVLGDAR
>Mature_249_residues
PAYDFTAPRLHVAADLAAGVILPLDRAQANYLLNVLRRGPDDPVLVFNGRHGEWRAHLVQTGRKGADLHVAAQTRPQTER
ADLHYLFAPLKSARLDYLAQKAVEMGAGTIRPVITRFTQGDRVNIDRLRANAVEAAEQCGILAIPECPEPERLPAALAGL
APGRLLVFCDEDAPVRDPVAALRAAGDPLAPPPLAVLVGPEGGFSEEERALILARPNTVALSLGPRILRADTAAVAVLTL
VQAVLGDAR

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=250, Percent_Identity=27.6, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 26687; Mature: 26556

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAYDFTAPRLHVAADLAAGVILPLDRAQANYLLNVLRRGPDDPVLVFNGRHGEWRAHLV
CCCCCCCCCCHHHHHHHHCCEEEECCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHH
QTGRKGADLHVAAQTRPQTERADLHYLFAPLKSARLDYLAQKAVEMGAGTIRPVITRFTQ
HCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
GDRVNIDRLRANAVEAAEQCGILAIPECPEPERLPAALAGLAPGRLLVFCDEDAPVRDPV
CCCCCHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCHH
AALRAAGDPLAPPPLAVLVGPEGGFSEEERALILARPNTVALSLGPRILRADTAAVAVLT
HHHHHCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCEEEEECCCEEEHHHHHHHHHHH
LVQAVLGDAR
HHHHHHCCCC
>Mature Secondary Structure 
PAYDFTAPRLHVAADLAAGVILPLDRAQANYLLNVLRRGPDDPVLVFNGRHGEWRAHLV
CCCCCCCCCHHHHHHHHCCEEEECCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHH
QTGRKGADLHVAAQTRPQTERADLHYLFAPLKSARLDYLAQKAVEMGAGTIRPVITRFTQ
HCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
GDRVNIDRLRANAVEAAEQCGILAIPECPEPERLPAALAGLAPGRLLVFCDEDAPVRDPV
CCCCCHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCHH
AALRAAGDPLAPPPLAVLVGPEGGFSEEERALILARPNTVALSLGPRILRADTAAVAVLT
HHHHHCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCEEEEECCCEEEHHHHHHHHHHH
LVQAVLGDAR
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]