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The map label for this gene is glnII [H]

Identifier: 170751508

GI number: 170751508

Start: 5450327

End: 5451361

Strand: Direct

Name: glnII [H]

Synonym: Mrad2831_5128

Alternate gene names: 170751508

Gene position: 5450327-5451361 (Clockwise)

Preceding gene: 170751505

Following gene: 170751511

Centisome position: 89.68

GC content: 65.31

Gene sequence:

>1035_bases
ATGACCAAATATAAGCTCGAGTACATATGGCTCGACGGGTACACGCCGACCCCGAATCTCCGCGGTAAGACGCAGATCAA
GGAATTCGACAGCTTCCCGACCCTCGAGCAGCTCCCGATGTGGGGCTTCGACGGCTCGTCCACCAAGCAGGCCGAGGGCG
GCAGCTCCGACTGCATGCTGAAGCCCGTGCGCCACTTCCCCGACCCGGCCCGCAAGAACGGCGTGCTGGTGATGTGCGAA
GTGATGATGCCGGACGGCGTGACCCCGCACGAGTCCAACAAGCGCGCCACCATCCTGGACGATGCCGGCGCCTGGTTCGG
CTTCGAGCAGGAGTACTTCCTGTACAAGGACGGCCGCCCGCTCGGCTTCCCGGCCTCCGGCTACCCGGCGCCGCAGGGCC
CGTACTACACCGGCGTCGGCTACTCGAACGTCGGCGACGTCGCCCGCAAGATCGTCGAGGAGCACCTCGACCTCTGCCTC
GATGCCGGCATCAACCACGAGGGCATCAACGCCGAGGTGGCCAAGGGCCAGTGGGAATTCCAGATCTTCGGCAAGGGCTC
CAAGAAGGCCGCCGACGAGATGTGGATGGCCCGCTACCTCCTGCAGCGCCTCTGCGAGAAGTACGGCATCGACGTCGAGT
ACCACTGCAAGCCGCTCGGCGACACCGACTGGAACGGGTCGGGCATGCACTGCAACTTCTCGACCGCGTTCATGCGCGAG
CACGGCGGCAAGGCCTATTTCGAGAAGCTCATGGAGGCATTCAAGAACGCCCGCGAGGAGCACATCGCCGTCTACGGTCC
GGACAACCACATGCGCCTGACCGGCAAGCACGAGACCGCCTCGATCCACGAGTTCTCGTACGGCGTGGCCGACCGCGGCG
CCTCGATCCGCGTGCCGCACTCCTTCGTCAACAACGGCTACAAGGGCTACCTGGAAGACCGCCGTCCGAACTCGCAGGGC
GACCCCTACCAGATCGCCTCGCAGGTGCTGAAGACGATCGCTTCCGTGCCGACCGAGGCCGCCGCAGCCGCGTAA

Upstream 100 bases:

>100_bases
GATCCATGCAAAGGCGGCTGCGGCCGTCTGAGAGGCGGCAAGCCGTTCGCGTTGCCCAGGGTTACCCCGGTCGCGCATCG
ATACGGACGAGAGAACCGGA

Downstream 100 bases:

>100_bases
GACGCGGGGGGAGGCGCCCAGTCTCCCGACAGAGCTAGCGAAGCCGGGCCCTCGGGTCCGGCTTCGCCGTTTCCGGGGCG
ATGGACTCCCGGGCAAGCGC

Product: glutamate--ammonia ligase

Products: NA

Alternate protein names: Glutamate--ammonia ligase II; Glutamine synthetase II; GSII [H]

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MTKYKLEYIWLDGYTPTPNLRGKTQIKEFDSFPTLEQLPMWGFDGSSTKQAEGGSSDCMLKPVRHFPDPARKNGVLVMCE
VMMPDGVTPHESNKRATILDDAGAWFGFEQEYFLYKDGRPLGFPASGYPAPQGPYYTGVGYSNVGDVARKIVEEHLDLCL
DAGINHEGINAEVAKGQWEFQIFGKGSKKAADEMWMARYLLQRLCEKYGIDVEYHCKPLGDTDWNGSGMHCNFSTAFMRE
HGGKAYFEKLMEAFKNAREEHIAVYGPDNHMRLTGKHETASIHEFSYGVADRGASIRVPHSFVNNGYKGYLEDRRPNSQG
DPYQIASQVLKTIASVPTEAAAAA

Sequences:

>Translated_344_residues
MTKYKLEYIWLDGYTPTPNLRGKTQIKEFDSFPTLEQLPMWGFDGSSTKQAEGGSSDCMLKPVRHFPDPARKNGVLVMCE
VMMPDGVTPHESNKRATILDDAGAWFGFEQEYFLYKDGRPLGFPASGYPAPQGPYYTGVGYSNVGDVARKIVEEHLDLCL
DAGINHEGINAEVAKGQWEFQIFGKGSKKAADEMWMARYLLQRLCEKYGIDVEYHCKPLGDTDWNGSGMHCNFSTAFMRE
HGGKAYFEKLMEAFKNAREEHIAVYGPDNHMRLTGKHETASIHEFSYGVADRGASIRVPHSFVNNGYKGYLEDRRPNSQG
DPYQIASQVLKTIASVPTEAAAAA
>Mature_343_residues
TKYKLEYIWLDGYTPTPNLRGKTQIKEFDSFPTLEQLPMWGFDGSSTKQAEGGSSDCMLKPVRHFPDPARKNGVLVMCEV
MMPDGVTPHESNKRATILDDAGAWFGFEQEYFLYKDGRPLGFPASGYPAPQGPYYTGVGYSNVGDVARKIVEEHLDLCLD
AGINHEGINAEVAKGQWEFQIFGKGSKKAADEMWMARYLLQRLCEKYGIDVEYHCKPLGDTDWNGSGMHCNFSTAFMREH
GGKAYFEKLMEAFKNAREEHIAVYGPDNHMRLTGKHETASIHEFSYGVADRGASIRVPHSFVNNGYKGYLEDRRPNSQGD
PYQIASQVLKTIASVPTEAAAAA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI74271826, Length=343, Percent_Identity=44.6064139941691, Blast_Score=266, Evalue=3e-71,
Organism=Homo sapiens, GI74271837, Length=343, Percent_Identity=44.6064139941691, Blast_Score=266, Evalue=3e-71,
Organism=Homo sapiens, GI19923206, Length=343, Percent_Identity=44.6064139941691, Blast_Score=266, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI115532978, Length=344, Percent_Identity=47.6744186046512, Blast_Score=287, Evalue=6e-78,
Organism=Caenorhabditis elegans, GI115532980, Length=344, Percent_Identity=47.6744186046512, Blast_Score=286, Evalue=8e-78,
Organism=Caenorhabditis elegans, GI17554028, Length=345, Percent_Identity=45.2173913043478, Blast_Score=270, Evalue=7e-73,
Organism=Caenorhabditis elegans, GI17540008, Length=344, Percent_Identity=44.4767441860465, Blast_Score=265, Evalue=4e-71,
Organism=Caenorhabditis elegans, GI17538856, Length=346, Percent_Identity=42.7745664739884, Blast_Score=261, Evalue=3e-70,
Organism=Caenorhabditis elegans, GI17551038, Length=317, Percent_Identity=39.1167192429022, Blast_Score=207, Evalue=6e-54,
Organism=Saccharomyces cerevisiae, GI6325292, Length=335, Percent_Identity=46.865671641791, Blast_Score=290, Evalue=3e-79,
Organism=Drosophila melanogaster, GI281360453, Length=344, Percent_Identity=46.5116279069767, Blast_Score=289, Evalue=2e-78,
Organism=Drosophila melanogaster, GI17136208, Length=344, Percent_Identity=46.5116279069767, Blast_Score=289, Evalue=2e-78,
Organism=Drosophila melanogaster, GI24580558, Length=344, Percent_Identity=46.5116279069767, Blast_Score=289, Evalue=2e-78,
Organism=Drosophila melanogaster, GI24641292, Length=340, Percent_Identity=48.2352941176471, Blast_Score=287, Evalue=7e-78,
Organism=Drosophila melanogaster, GI24641290, Length=340, Percent_Identity=48.2352941176471, Blast_Score=287, Evalue=8e-78,
Organism=Drosophila melanogaster, GI45554830, Length=268, Percent_Identity=49.6268656716418, Blast_Score=238, Evalue=3e-63,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 38469; Mature: 38337

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKYKLEYIWLDGYTPTPNLRGKTQIKEFDSFPTLEQLPMWGFDGSSTKQAEGGSSDCML
CCCEEEEEEEECCCCCCCCCCCCCHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCCCEE
KPVRHFPDPARKNGVLVMCEVMMPDGVTPHESNKRATILDDAGAWFGFEQEYFLYKDGRP
HHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCC
LGFPASGYPAPQGPYYTGVGYSNVGDVARKIVEEHLDLCLDAGINHEGINAEVAKGQWEF
CCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCEEEE
QIFGKGSKKAADEMWMARYLLQRLCEKYGIDVEYHCKPLGDTDWNGSGMHCNFSTAFMRE
EEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEEECCHHHHHHH
HGGKAYFEKLMEAFKNAREEHIAVYGPDNHMRLTGKHETASIHEFSYGVADRGASIRVPH
CCCHHHHHHHHHHHHCCCCCEEEEECCCCCEEEECCCCCCCHHHHHCCCCCCCCEEECCH
SFVNNGYKGYLEDRRPNSQGDPYQIASQVLKTIASVPTEAAAAA
HHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCC
>Mature Secondary Structure 
TKYKLEYIWLDGYTPTPNLRGKTQIKEFDSFPTLEQLPMWGFDGSSTKQAEGGSSDCML
CCEEEEEEEECCCCCCCCCCCCCHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCCCEE
KPVRHFPDPARKNGVLVMCEVMMPDGVTPHESNKRATILDDAGAWFGFEQEYFLYKDGRP
HHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCC
LGFPASGYPAPQGPYYTGVGYSNVGDVARKIVEEHLDLCLDAGINHEGINAEVAKGQWEF
CCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCEEEE
QIFGKGSKKAADEMWMARYLLQRLCEKYGIDVEYHCKPLGDTDWNGSGMHCNFSTAFMRE
EEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEEECCHHHHHHH
HGGKAYFEKLMEAFKNAREEHIAVYGPDNHMRLTGKHETASIHEFSYGVADRGASIRVPH
CCCHHHHHHHHHHHHCCCCCEEEEECCCCCEEEECCCCCCCHHHHHCCCCCCCCEEECCH
SFVNNGYKGYLEDRRPNSQGDPYQIASQVLKTIASVPTEAAAAA
HHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]