Click here to switch to the map view.

The map label for this gene is polA [H]

Identifier: 170751505

GI number: 170751505

Start: 5446939

End: 5447562

Strand: Direct

Name: polA [H]

Synonym: Mrad2831_5125

Alternate gene names: 170751505

Gene position: 5446939-5447562 (Clockwise)

Preceding gene: 170751503

Following gene: 170751508

Centisome position: 89.62

GC content: 70.99

Gene sequence:

>624_bases
ATGCCGAGCCCGACCGTCCGTCTTCACCGCGGCGACCTGCCCGCCAGCTACGACGCGGGCCGGGCGGTGGCCATCGACAC
CGAGACGCTCGGGCTCAACCCGCATCGGGACCGCCTCTGCGTGGTGCAGCTCTCCACCGGCGACGGCACCGCCGACGTGG
TCCAGATCCCGCAAGCCGGGCCGGAGCCGGTCGTGCTCAAACGGGTCCTGGCCGATCCCGGCATCCTGAAGATCTTCCAC
TTCGCGCGGTTCGACGTGGCGGTCCTGTACAGGGCCCTCGGCGTGATGCCGAGCCCGGTCTACTGCACCAAGATCGCCTC
GAAGCTCGCGCGCACCTACACGGACCGCCACGGGCTGAAGGACGTGGTCCGCGAGCTGGTCGGCGTCGACCTGTCGAAGC
AGCAGCAATCCTCGGACTGGGGCGCCGAGACCCTGTCGCAGGCCCAGCTCGACTACGCGGCCTCGGATGTCCTCCACCTC
CACGCGGCCCGGGAGCGGCTCGACGCCATGCTGGCCCGGGAAGGCCGCACCGATCTCGCGGCCGCCTGTTTCGGCTTCCT
GCCGACGCGGGCGCGCCTCGACCTCGACGGCTGGCCCGAGACTGACATCTTCGCGCACACCTGA

Upstream 100 bases:

>100_bases
TGTCCAGCTCCCTGTCGGTCCCGGCCGCGCGAATCGTCCGCGGCTTGCCGGCACTTGGCACCGATGCTACCGGCCGAAAA
CCCCGCAAGGTCCGAGAACG

Downstream 100 bases:

>100_bases
GAGTCGCGAGGATCCCGCACACTTCCTGTGCGGGGTGAGACTTTCCTGTCACCGCACCGCGATGTGTCGCTGGTAGGTTG
TGGCTGCCTGGGCTTGCGAG

Product: 3'-5' exonuclease

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MPSPTVRLHRGDLPASYDAGRAVAIDTETLGLNPHRDRLCVVQLSTGDGTADVVQIPQAGPEPVVLKRVLADPGILKIFH
FARFDVAVLYRALGVMPSPVYCTKIASKLARTYTDRHGLKDVVRELVGVDLSKQQQSSDWGAETLSQAQLDYAASDVLHL
HAARERLDAMLAREGRTDLAAACFGFLPTRARLDLDGWPETDIFAHT

Sequences:

>Translated_207_residues
MPSPTVRLHRGDLPASYDAGRAVAIDTETLGLNPHRDRLCVVQLSTGDGTADVVQIPQAGPEPVVLKRVLADPGILKIFH
FARFDVAVLYRALGVMPSPVYCTKIASKLARTYTDRHGLKDVVRELVGVDLSKQQQSSDWGAETLSQAQLDYAASDVLHL
HAARERLDAMLAREGRTDLAAACFGFLPTRARLDLDGWPETDIFAHT
>Mature_206_residues
PSPTVRLHRGDLPASYDAGRAVAIDTETLGLNPHRDRLCVVQLSTGDGTADVVQIPQAGPEPVVLKRVLADPGILKIFHF
ARFDVAVLYRALGVMPSPVYCTKIASKLARTYTDRHGLKDVVRELVGVDLSKQQQSSDWGAETLSQAQLDYAASDVLHLH
AARERLDAMLAREGRTDLAAACFGFLPTRARLDLDGWPETDIFAHT

Specific function: Cleaves Multimeric tRNA Precursor At The Spacer Region. [C]

COG id: COG0349

COG function: function code J; Ribonuclease D

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 3'-5' exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1788105, Length=142, Percent_Identity=33.8028169014084, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR002298
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01612 3_5_exonuc; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 22625; Mature: 22494

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSPTVRLHRGDLPASYDAGRAVAIDTETLGLNPHRDRLCVVQLSTGDGTADVVQIPQAG
CCCCCEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCEEEEECCCCC
PEPVVLKRVLADPGILKIFHFARFDVAVLYRALGVMPSPVYCTKIASKLARTYTDRHGLK
CCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHH
DVVRELVGVDLSKQQQSSDWGAETLSQAQLDYAASDVLHLHAARERLDAMLAREGRTDLA
HHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
AACFGFLPTRARLDLDGWPETDIFAHT
HHHHHHCCCCEEECCCCCCCCCCEECC
>Mature Secondary Structure 
PSPTVRLHRGDLPASYDAGRAVAIDTETLGLNPHRDRLCVVQLSTGDGTADVVQIPQAG
CCCCEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCEEEEECCCCC
PEPVVLKRVLADPGILKIFHFARFDVAVLYRALGVMPSPVYCTKIASKLARTYTDRHGLK
CCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHH
DVVRELVGVDLSKQQQSSDWGAETLSQAQLDYAASDVLHLHAARERLDAMLAREGRTDLA
HHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
AACFGFLPTRARLDLDGWPETDIFAHT
HHHHHHCCCCEEECCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]