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The map label for this gene is gsiB [H]

Identifier: 170751464

GI number: 170751464

Start: 5399922

End: 5401409

Strand: Reverse

Name: gsiB [H]

Synonym: Mrad2831_5083

Alternate gene names: 170751464

Gene position: 5401409-5399922 (Counterclockwise)

Preceding gene: 170751472

Following gene: 170751463

Centisome position: 88.87

GC content: 67.94

Gene sequence:

>1488_bases
ATGTTGCGTCTCGCCGCCTGTCTCCTCGCCGGGTTCGTCCTCGCCGGCCCCGCCGCCGCGCAGACCCTGCGCTACGGCAT
GATGGAGGATCCGGACGCCCTCGACCCGACGCTCGCCCGGACCTTCGCGAGCCGGATGGTGTTCGCGGCGCTCTGCGACA
AGCTCGTGGATATCGGGCCCGATCTGAAGCCGGTGCCGCAGCTCGCCACCTCCTGGGAGCTGTCGCCGGACGAGAAGGCG
CTGACCATGCACCTGCGCCCGGGTGTCCTGTTCCACGACGGCGAGAAGCTCGACGCCGCGGCGGCCAAGTTCTCGATCGA
GCGGCACATGAAGATGCCGGGCTCGCAGCGCCGCTCGGAGATCGCGCCGATCGACTCGGTGGACGTCGTCGACGACCTGA
CCTTCCGGATCAACCTGAAGCAGCCCTTCGCGCCGCTGCTGGCGCAGTTCACCGACCGGGCCGGCATGATGGTCTCACCC
AAGGCCGCCGAGCGGCTCGGCGACAAGTTCGCCACCGCGCCGGTCTGCGCCGGTCCGTACAAGTTCGTGGAGCGGGTGCC
GCAGGACCGGATCGTGGTCGAGCGCTTCCCCGACTACTGGAACAAGGACGCGATCCACATCCAGCGGATCGAGTTCCGCC
CGATCCCCGACGCCACCGTGCGGCTCACGAACCTGCGCGCCGGGCAGCTCGACCTGCTGGAGCGGCTCGCCCCGACGGAC
GTGGCGCAGGTGAAGCGCGACGCCAAGCTGAAGCTCGATTCGACCACCGAGCTCGGCTTCCAGGAGATCCTGTTCAACCC
CAACAAGGCCGGCTCGCCGGTTTCCGACCCGAAGGTGCGCGCCGCCTTCGAGGCCGCCATCGACCGCAACGCCATCAATC
AGGTGGTCTATAACGGCGAGTTCCTGGCCGGGAACCAGTGGGTCAACCCGAAGAACCCGAACTACGTCAGCGAGTACCCG
ATCCCCAAGCGAGACGTCGCCAAGGCGAAGGCCCTGCTCGCCGAAGCCGGCAAGCCGAACCCGCAGATCACCCTGACCGT
CTACGCCAACAGCGAGTCGCCGCAGGTGGGCCAGGTGATCCAGGCCATGACCAAGGAGGCGGGCTTCGACGTGAAGATCC
AGGCCGTGGACTTCACCACCGCGCTCGATGCCGCCGACAAGGGCAATTACGAGGCGCAGCTCTATTCCTGGAGCGGCCGC
TCGGACCCGGACGGCAACACGTTCAGCTTCCTGGCCTGCAAGGCGGCGCTGAACTATCCCAAGTACTGCAGCCCCGAGGC
CGACGCGGCGCTCCAGGCCGAGCGCGGCACCGTCGATCCGGCCAAGCGCAAGGCCGCCTGGAAGGCGCTCGCCGACCAAG
TCCTGAAGGACCGGCCGGGCATCTACATCCTGCACCGCAAGCTGCTCTGGGCCTACAGCCAGAAGATCACGGGCTTCGTG
CCCTACCCGGACGGGCTGGTGCGCTTCACCGGGCTGAAGCTGAACTGA

Upstream 100 bases:

>100_bases
GGATTGTGCATGCCACACGGCCGCGCAACACTGCCGGTCGCCCCTGGAACGGACGCGGCCGATCCGCACCGCTCCTCCCG
CCTCCATCCGGACCGGTCTC

Downstream 100 bases:

>100_bases
TGCTGCGGTTCCTGGCGCGTCGCCTCGCCCTGGCGGTCCCGACCCTGATCCTCGCCTCGATGATCATCTTCGCCCTGCAG
CAATTGCTGCCGGGCGACGT

Product: 4-phytase

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MLRLAACLLAGFVLAGPAAAQTLRYGMMEDPDALDPTLARTFASRMVFAALCDKLVDIGPDLKPVPQLATSWELSPDEKA
LTMHLRPGVLFHDGEKLDAAAAKFSIERHMKMPGSQRRSEIAPIDSVDVVDDLTFRINLKQPFAPLLAQFTDRAGMMVSP
KAAERLGDKFATAPVCAGPYKFVERVPQDRIVVERFPDYWNKDAIHIQRIEFRPIPDATVRLTNLRAGQLDLLERLAPTD
VAQVKRDAKLKLDSTTELGFQEILFNPNKAGSPVSDPKVRAAFEAAIDRNAINQVVYNGEFLAGNQWVNPKNPNYVSEYP
IPKRDVAKAKALLAEAGKPNPQITLTVYANSESPQVGQVIQAMTKEAGFDVKIQAVDFTTALDAADKGNYEAQLYSWSGR
SDPDGNTFSFLACKAALNYPKYCSPEADAALQAERGTVDPAKRKAAWKALADQVLKDRPGIYILHRKLLWAYSQKITGFV
PYPDGLVRFTGLKLN

Sequences:

>Translated_495_residues
MLRLAACLLAGFVLAGPAAAQTLRYGMMEDPDALDPTLARTFASRMVFAALCDKLVDIGPDLKPVPQLATSWELSPDEKA
LTMHLRPGVLFHDGEKLDAAAAKFSIERHMKMPGSQRRSEIAPIDSVDVVDDLTFRINLKQPFAPLLAQFTDRAGMMVSP
KAAERLGDKFATAPVCAGPYKFVERVPQDRIVVERFPDYWNKDAIHIQRIEFRPIPDATVRLTNLRAGQLDLLERLAPTD
VAQVKRDAKLKLDSTTELGFQEILFNPNKAGSPVSDPKVRAAFEAAIDRNAINQVVYNGEFLAGNQWVNPKNPNYVSEYP
IPKRDVAKAKALLAEAGKPNPQITLTVYANSESPQVGQVIQAMTKEAGFDVKIQAVDFTTALDAADKGNYEAQLYSWSGR
SDPDGNTFSFLACKAALNYPKYCSPEADAALQAERGTVDPAKRKAAWKALADQVLKDRPGIYILHRKLLWAYSQKITGFV
PYPDGLVRFTGLKLN
>Mature_495_residues
MLRLAACLLAGFVLAGPAAAQTLRYGMMEDPDALDPTLARTFASRMVFAALCDKLVDIGPDLKPVPQLATSWELSPDEKA
LTMHLRPGVLFHDGEKLDAAAAKFSIERHMKMPGSQRRSEIAPIDSVDVVDDLTFRINLKQPFAPLLAQFTDRAGMMVSP
KAAERLGDKFATAPVCAGPYKFVERVPQDRIVVERFPDYWNKDAIHIQRIEFRPIPDATVRLTNLRAGQLDLLERLAPTD
VAQVKRDAKLKLDSTTELGFQEILFNPNKAGSPVSDPKVRAAFEAAIDRNAINQVVYNGEFLAGNQWVNPKNPNYVSEYP
IPKRDVAKAKALLAEAGKPNPQITLTVYANSESPQVGQVIQAMTKEAGFDVKIQAVDFTTALDAADKGNYEAQLYSWSGR
SDPDGNTFSFLACKAALNYPKYCSPEADAALQAERGTVDPAKRKAAWKALADQVLKDRPGIYILHRKLLWAYSQKITGFV
PYPDGLVRFTGLKLN

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=535, Percent_Identity=28.0373831775701, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1787052, Length=506, Percent_Identity=26.8774703557312, Blast_Score=152, Evalue=6e-38,
Organism=Escherichia coli, GI1787495, Length=528, Percent_Identity=25.5681818181818, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI87081878, Length=494, Percent_Identity=26.1133603238866, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1787762, Length=504, Percent_Identity=24.6031746031746, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1787551, Length=477, Percent_Identity=24.7379454926625, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1789887, Length=497, Percent_Identity=25.5533199195171, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1789397, Length=494, Percent_Identity=22.4696356275304, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 54587; Mature: 54587

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRLAACLLAGFVLAGPAAAQTLRYGMMEDPDALDPTLARTFASRMVFAALCDKLVDIGP
CHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
DLKPVPQLATSWELSPDEKALTMHLRPGVLFHDGEKLDAAAAKFSIERHMKMPGSQRRSE
CCCCHHHHHHCCCCCCCCCEEEEEECCCEEEECCCHHHHHHHHHHHHHHHCCCCCHHHCC
IAPIDSVDVVDDLTFRINLKQPFAPLLAQFTDRAGMMVSPKAAERLGDKFATAPVCAGPY
CCCCCCCCHHCCEEEEEECCCCHHHHHHHHHHCCCCEECCHHHHHHHHHHCCCCCCCCHH
KFVERVPQDRIVVERFPDYWNKDAIHIQRIEFRPIPDATVRLTNLRAGQLDLLERLAPTD
HHHHHCCCCCCHHHHCCCCCCCCCEEEEEEEEECCCCCEEEEECCCCCHHHHHHHHCCCC
VAQVKRDAKLKLDSTTELGFQEILFNPNKAGSPVSDPKVRAAFEAAIDRNAINQVVYNGE
HHHHHCCCEEEECCCHHCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCE
FLAGNQWVNPKNPNYVSEYPIPKRDVAKAKALLAEAGKPNPQITLTVYANSESPQVGQVI
EEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHH
QAMTKEAGFDVKIQAVDFTTALDAADKGNYEAQLYSWSGRSDPDGNTFSFLACKAALNYP
HHHHHHCCCCEEEEEEEEHHHHCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEHHHCCCC
KYCSPEADAALQAERGTVDPAKRKAAWKALADQVLKDRPGIYILHRKLLWAYSQKITGFV
CCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCEEC
PYPDGLVRFTGLKLN
CCCCCEEEEECEECC
>Mature Secondary Structure
MLRLAACLLAGFVLAGPAAAQTLRYGMMEDPDALDPTLARTFASRMVFAALCDKLVDIGP
CHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
DLKPVPQLATSWELSPDEKALTMHLRPGVLFHDGEKLDAAAAKFSIERHMKMPGSQRRSE
CCCCHHHHHHCCCCCCCCCEEEEEECCCEEEECCCHHHHHHHHHHHHHHHCCCCCHHHCC
IAPIDSVDVVDDLTFRINLKQPFAPLLAQFTDRAGMMVSPKAAERLGDKFATAPVCAGPY
CCCCCCCCHHCCEEEEEECCCCHHHHHHHHHHCCCCEECCHHHHHHHHHHCCCCCCCCHH
KFVERVPQDRIVVERFPDYWNKDAIHIQRIEFRPIPDATVRLTNLRAGQLDLLERLAPTD
HHHHHCCCCCCHHHHCCCCCCCCCEEEEEEEEECCCCCEEEEECCCCCHHHHHHHHCCCC
VAQVKRDAKLKLDSTTELGFQEILFNPNKAGSPVSDPKVRAAFEAAIDRNAINQVVYNGE
HHHHHCCCEEEECCCHHCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCE
FLAGNQWVNPKNPNYVSEYPIPKRDVAKAKALLAEAGKPNPQITLTVYANSESPQVGQVI
EEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHH
QAMTKEAGFDVKIQAVDFTTALDAADKGNYEAQLYSWSGRSDPDGNTFSFLACKAALNYP
HHHHHHCCCCEEEEEEEEHHHHCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEHHHCCCC
KYCSPEADAALQAERGTVDPAKRKAAWKALADQVLKDRPGIYILHRKLLWAYSQKITGFV
CCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCEEC
PYPDGLVRFTGLKLN
CCCCCEEEEECEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA